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Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice (벼물바구미 (Lissorhoptrus oryzophilus) 내충성 GM 벼에서 T-DNA와 게놈의 인접부위 분석)

  • Lee, Jin-Hyoung;Shin, Kong-Sik;Suh, Seok-Cheol;Rhim, Seong-Lyul;Lim, Myung-Ho;Woo, Hee-Jong;Qin, Yang;Kweon, Soon-Jong;Park, Soon-Ki
    • Journal of Plant Biotechnology
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    • v.41 no.3
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    • pp.127-133
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    • 2014
  • Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insertion number of foreign genes, and analyzed the flanking sequences of T-DNAs in rice genome. As a result, T-DNA of BT12R1 line was inserted in exon region of rice chromosome 10. Two copies of T-DNAs were inserted in line BT12R2. BT12R3 line was analyzed at only left border flanking sequence. BT12R4 line was confirmed one copy of foreign gene insertion at the position 24,516,607 ~ 24,516,636 of rice chromosome 5, accompanied by a deletion of 30 bp known genomic sequences. This intergenic position was confirmed none of expressed gene and any deletion/addition of T-DNA sequence. In conclusion, these molecular data of rice water weevil resistant Bt rice would be used to conduct the biosafety and environment risk assessment for GM crop commercialization.

Use of ultrasonography for improving reproductive efficiency in cows II. Comparative evaluation of ovarian structures using ultrasonography and plasma progesterone analysis in subestrous dairy cows (초음파 진단장치를 이용한 축우의 번식효율증진에 관한 연구 II. 무발정 젖소에서 초음파검사 및 progesterone 농도측정에 의한 난소 구조물의 비교평가)

  • Son, Chang-ho;Kang, Byong-kyu;Choi, Han-sun;Kang, Hyun-gu;Paik, In-seok;Suh, Guk-hyun
    • Korean Journal of Veterinary Research
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    • v.38 no.3
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    • pp.642-651
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    • 1998
  • The accuracy of ultrasonography for determining the presence of a functional corpus luteum in subestrous dairy cows was investigated, using a radioimmunoassay for progesterone in plasma. Luteal status (high or low progesterone concentrations) was diagnosed in 534 cows, using B-mode transrectal ultrasonography. Accuracy of ultrasonography was 96.3% and 88.8% in the cows with and without functional corpus luteum, respectively. In 362 cows diagnosed with functional corpus luteum by ultrasonographic examination, 20 cows were diagnosed with the non-functional corpus luteum by analysis of plasma progesterone concentrations (false positive). In 172 cows with non-functional corpus luteum by ultrasonographic examination, 13 cows were diagnosed with the functional corpus luteum based on plasma progesterone assay (false negative). Most of the corpus luteum with well-defined border and homogeneous echotexture were diagnosed with functional corpus luteum. All cows that were not detected a corpus luteum by ultrasonographic examination were diagnosed as non-functional corpus luteum. The corpus luteum of cows that were diagnosed with false positive appeared homogeneous echotexture and above 15 mm in diameter, but the corpus luteum was the non-functional corpus luteum within Day 5 (Day 0 is ovulation day) or after Day 19. The corpus luteum of cows that were diagnosed with false negative appeared heterogeneous echogenicity and below 15 mm in diameter, but the corpus luteum was the functional corpus luteum after Day 5 or around Day 17. It was concluded that accuracy of ultrasonography was excellent for determining the presence of a functional corpus luteum in subestrous dairy cows. The corpus luteum that was diagnosed with false positive or false negative was the developing or regressing states. Thus, ultrasonography was required a serial examination of two or three times accurately diagnosing these corpus luteum.

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The Estimation of Temporal Change Patterns associated with Economic Growth and Urban Areas in a Border Region using DMSP-OLS Nighttime Imagery Data: The Case Study of Jilin Province, China (DMSP-OLS 야간영상자료를 이용한 접경지역의 경제성장과 시가지 면적의 시계열 변화 패턴 추정: 중국 지린성을 사례로)

  • Kim, Minho;Joh, Young-Kug
    • Journal of the Economic Geographical Society of Korea
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    • v.22 no.4
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    • pp.458-471
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    • 2019
  • DMSP-OLS nighttime satellite imagery could be used to derive the sum of lights (SOL) and built-up area, and the two indices have been widely employed to make the estimation of socio-economic variables and the dynamics of urban developments. Considering it, this research investigated the spatiotemporal patterns of economic growth and urbanized area in Jilin Province, China, using DMSP-OLS data for a time span between 1992 and 2012. This study found the SOLs of both the province and most cities to tend to grow during the period. While SOL-weighted centroids' means moved towards northwestern direction, urban-area centroids' means followed the trend of south-eastern migration. These directional patterns could be associated with the Northeast Revitalization Plan of Chinese governments. Nonetheless, a future study will need to consider SNPP VIIRS DNB imagery in order to overcome temporal limitation of DMSP-OLS data. In addition, it is also necessary to estimate socio-economic indices, e.g., growth regional domestic product, using a regression model developed with correlation relationship between economic statistics ad SOL.

The Distribution and Characteristics of Geographical Names on 1:50,000 Topographic Map of the Korean Peninsula in the Early 20th Century ("오만분일지형도(五萬分一地形圖)"에 나타난 20세기 초 한반도의 지명 분포와 특성)

  • Kim, Sun-Hee
    • Journal of the Korean Geographical Society
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    • v.43 no.1
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    • pp.87-103
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    • 2008
  • The geographical name is an expression of human knowledge about living conditions and a basic tool for understanding about regional cultures and history as a result of spatiotemporal changes. This study aims to understand the historical and regional characteristics by analyzing the frequency and distribution of geographical names of 1:50,000 topographic map in the early 20th century and materializing on electronic cultural atlas. The result of this analysis is as follows. First, the aspect of distributed geographical names on the Korean Peninsula reflects a general trait of the country's natural and human environment included geographical features, population, arable land, the number of counties and villages, and functions of administration and military, etc. Second, through the frequency and weight of their names, the analysis shows not only the change of native names, but also the country's phase of the time by Japanese colonial policies such as exploitation of resources, the construction of railroads, and a desire to control of the border area with Manchuria. In addition, the study identified regional characteristics and differences in environmental perception and preferences, and naming basis and forms by the comparative analysis of each type of geographical names associated with village and ridge such as 'Chi' 'Ryung' 'Duk' 'Hang' 'Pyeong' 'Gok'. In particular, those characteristics were largely affected from environmental difference of each area.

Periodontal Wound Healing of the Experimental Subepithelial Connective Tissue Graft in Dogs (성견의 실험적 상피하 결합조직 이식시의 치주조직의 치유)

  • Jung, Hyun-Chul;Choi, Seong-Ho;Cho, Kyoo-Sung;Chai, Jung-Kyi;Kim, Chong-Kwan
    • Journal of Periodontal and Implant Science
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    • v.27 no.2
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    • pp.379-394
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    • 1997
  • Several methods have been used for regeneration of tissue lost by periodontal disease. Subepithelial connective tissue graft technique, one of the technniques of mucogingival surgery, is used for the regeneration in esthetic problems such as recession, and denuded root coverage. This study is performed to evaluate the healing process and the regeneration and reattachment of periodontal tissue, including the reconstruction of junctional epithelium, and connective tissue. Alveolar defects in five adult dogs were treated with periodontal surgery and were attained by removing the marginal alveolar bone by $4{\time}3mm$ from CEJ in the labial side of incisors, and root surfaces were planed. The experimental sites were divided into two groups as follows. 1. root planing alone(control group) 2. with connective tissue graft (Experimental Group) In the two groups flaps were positioned and sutured tightly, the healing processes were observed and were histologically compared with each other after 2days, 4days, 1week, 2weeks, 4weeks. The results were obtained as follows : 1. In the two groups blood clots were observed as early as 2 and 4 days, and were resorbed at 1 week. 2. In the two groups moderate inflammation was observed as early as 2 and 4 days, decreased at 1 and 2 weeks, and disappeared at 4 weeks. 3. Junctional Epithelium migration was more significant in the control group, and was restrained by graft materials in the experimental group. 4. Features of connective tissue fiber attachment partially showed the parallel pattern in the two groups from 2 weeks, and entirely from 4weeks. 5. Anastomosis, between graft and connective tissue, appeared from 4 days in the experimental group and the border between them was not discriminated at 4weeks.

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Successful Conservative Surgical Treatment of Ameloblastic Fibroma in the Posterior Maxilla : A Case Report (상악 구치부에 발생한 법랑모세포섬유종의 성공적인 보존적 수술 : 증례 보고)

  • Lee, Youngeun;Ahn, Hyojung;Lee, Sooeon;Kim, Euncheol;Choi, Sungchul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.40 no.4
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    • pp.321-327
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    • 2013
  • Ameloblastic fibroma (AF) is a rare odontogenic ectomesenchymal tumor that is frequently seen in the first two decades of life, and occurs in the mandible. The most proper management of AF has been a recent topic of debate because of its recurrence and malignant transformation. This report describes AF in a 4-year-old male, which was a unilocular radiolucency on the maxillary right primary molar area with a scalloped border and corticated margin. The tumor was treated conservatively with enucleation and curettage, and the decision was made to preserve the right primary second molar. A biopsy confirmed it as AF. During the 43 months of follow-up, the patient had no evidence of recurrence or malignant transformation. Moreover, the radiographic examination revealed the generation of tooth germ to be a permanent second premolar. This report shows a case of AF in the posterior maxilla of a 4-year-old boy and discusses the conservative therapeutic approach to this tumor. Therefore, the age of the patients should be an important consideration when choosing conservative or radical surgery in a young AF patient.

한국원자력연구소 방사선방어기술 개발 및 연구 현황

  • Ha, Jeong-U
    • Journal of Radiation Protection and Research
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    • v.15 no.1
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    • pp.9-13
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    • 1990
  • 1959년 한국원자력연구소가 창립됨과 동시에 &Health Physics&, 즉 보건물리라고 하는 명칭과 조직이 탄생되어, 방사선안전관리의 실무와 보건물리의 연구가 시작되었다. 최초 10년간은 선진제국의 보건물리분야의 연구와 기술을 추적하여 우리나라의 방사선안전관리 기술의 기초를 다지는 시기로서 개인방사선모니터링기술, 환경방사선(능) 모니터링기술 및 방사선방어용계측기기의 교정기술 개발에 중점을 두고 연구개발이 추진되었으며, TRIGA Mark-II 연구용원자로의 가동에 따라 원자로 생체차폐체의 건전성 검증에 관한 유익한 방사선량 측정자료도 얻게 되었다. 즉 이 기간은 방사선안전관리의 체제정비 및 기초기술 확립에 노력한 기간이었다. 1970년대는 원자력 연구개발에 대한 기본방향과 정책의 변경등으로 보건물리 연구조직은 방사선안전관리, 환경연구 그리고 방사화학분야로 분산되었으며, 그로인하여 연구개발활동은 거의 정체되어 겨우 방사선안전관리 실무만이 그 명맥을 유지하였다. 그 결과 우리나라 방사선안전관리 및 그와 관련된 연구개발의 기반이 흔들리게 되었으나, 그러한 환경하에서도 방사선량측정평가기술, 방사선차폐설계기술 및 원자로사고시 피폭선량평가기술의 선진화에 필요한 지식을 얻었으며, 방사선 안전관리에 유익한 실무경험도 축적하게 되었다. 1980년대는 통합된 원자력 연구개발체제의 구축으로 방사선작업종사자 및 일반공중의 피폭저감화 기술개발에 필요한 각종 최신기술을 도입하였고, 관리업무에 있어서도 측정의 정확도와 신뢰성향상 및 새로운 관리기술의 개발에 많은 노력을 한 결과, 유익한성과를 얻게된 기간이다. 특히, 이 기간은 방사선안전관리기술의 선진화를 위한 지식이 축적되어 90년대의 방사선안전관리기술자립화를 위한 전환기로서, 이와같이 축적된 기술은 원자력의 평화적 이용에 크게 기여할 것으로 기대된다.서 dithiothreitol를 투여한 군에서는 우라늄단독투여군에 비해 cretinine의 배설이 상당히 증가하였다(P<0.05). 6. 우라늄오염에 의한 신장의 소견에 있어 우라늄단독투여군은 근위곡세뇨관상피의 공포화 및 종창, microvilli와 brush border의 손실, 세뇨관 상피의 괴사가 관찰되었으며, 간장의 충혈, 중심성 괴사 및 모세관 확장증도 관찰되었다. 그리고 sodium bicarbonate와 생리적 식염수를 병행투여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군에서는 우라늄 단독투여군에 비해 높은 방호효과가 관찰되었으나 다른 실험군에서는 큰 효과가 없는 것으로 나타났다. 결론적으로 우라늄의 체내오염시에는 sodium bicarbonate와 생리적 식염수를 가능한 빨리 병행투여하거나 dithiothreitol을 체내오염후 30분이 지나서 투여하는 방법이 우라늄오염에 대한 제염에 매우 유효할 것으로 생각되며, 특히 우라늄에 의한 인체장해를 유의하게 경감시켜줄 것으로 사료되었다.내의 어떤 부위와도 관계가 되는 것으로 간주되는데 이것이 $(^3H)$ QNB가 $(^3H)$ NMS보다 높은 최대 결합능력 $(B_{max})$을 나타낼 이유이다. (b) 두 종류의 다른 제제에서 우리는 같은 양상의 결과를 관찰하었기에 결점이 많은 homogenates 제제보다는 intact cell aggregates 제제를 수용체 연구에 대한 새로운 실험모형(experiment model)으로 사용할 수 있는 가능성을 제시하고자 한다.$가 38.8%로 가장 많고, 그 다음이 ${\ulcorner}$l9세(歲)이후${\lrcorner}$가 25.2%로서 전체

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Evaluation of Source Identification Method Based on Energy-Weighting Level with Portal Monitoring System Using Plastic Scintillator

  • Lee, Hyun Cheol;Koo, Bon Tack;Choi, Chang Il;Park, Chang Su;Kwon, Jeongwan;Kim, Hong-Suk;Chung, Heejun;Min, Chul Hee
    • Journal of Radiation Protection and Research
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    • v.45 no.3
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    • pp.117-129
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    • 2020
  • Background: Radiation portal monitors (RPMs) involving plastic scintillators installed at the border inspection sites can detect illicit trafficking of radioactive sources in cargo containers within seconds. However, RPMs may generate false alarms because of the naturally occurring radioactive materials. To manage these false alarms, we previously suggested an energy-weighted algorithm that emphasizes the Compton-edge area as an outstanding peak. This study intends to evaluate the identification of radioactive sources using an improved energy-weighted algorithm. Materials and Methods: The algorithm was modified by increasing the energy weighting factor, and different peak combinations of the energy-weighted spectra were tested for source identification. A commercialized RPM system was used to measure the energy-weighted spectra. The RPM comprised two large plastic scintillators with dimensions of 174 × 29 × 7 ㎤ facing each other at a distance of 4.6 m. In addition, the in-house-fabricated signal processing boards were connected to collect the signal converted into a spectrum. Further, the spectra from eight radioactive sources, including special nuclear materials (SNMs), which were set in motion using a linear motion system (LMS) and a cargo truck, were estimated to identify the source identification rate. Results and Discussion: Each energy-weighted spectrum exhibited a specific peak location, although high statistical fluctuation errors could be observed in the spectrum with the increasing source speed. In particular, 137Cs and 60Co in motion were identified completely (100%) at speeds of 5 and 10 km/hr. Further, SNMs, which trigger the RPM alarm, were identified approximately 80% of the time at both the aforementioned speeds. Conclusion: Using the modified energy-weighted algorithm, several characteristics of the energy weighted spectra could be observed when the used sources were in motion and when the geometric efficiency was low. In particular, the discrimination between 60Co and 40K, which triggers false alarms at the primary inspection sites, can be improved using the proposed algorithm.

The Impact of M&A of Small and Medium-Sized Companies on Merger Disclosure Effect and Long-Term Operating Performance (중소기업의 M&A가 합병 공시효과와 장기 영업성과에 미치는 영향)

  • Kim, Byoung_jin;Jung, Jin-young
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.12 no.6
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    • pp.49-63
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    • 2017
  • The purpose of this study is to analyze the effects of the collective characteristics of SMEs on M&A activity on the disclosure effect of mergers and long-term business performance after mergers. From 2000 to 2012, we examine 717 cases of small and medium-sized enterprises (SMEs) using multiple regression analysis and difference analysis. The results of this study are as follows. First, it is confirmed that the effect on the merger announcement effect is the same as the previous study on the Korean capital market listed companies except for the effect of diversification, listing effect, and cross-border effect. In addition, we have found that firms with higher performance in the past have higher excess returns in the disclosure effect of mergers and acquisitions. Second, unlike the previous studies that non-related mergers have a positive effect on long-term operating performance, for the characteristics of SMEs with lower market competitiveness than that of average listed companies, SMEs merging with same industry group companies have a positive effect on long-term operating performance. This study provides a new perspective on the merger and acquisition of SMEs by examining the effects of M&A announcement and long-term performance.

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Arthroscopic Medial Plication using Pull-out Suture for the Treatment of Acute or Recurrent Patellar Dislocation - Technical Note - (급성 또는 재발성 슬개골 탈구의 치료에 있어서 견인 봉합술을 이용한 관절경적 내측 관절막 중첩술 - 수술 술기 -)

  • Ahn, Jin-Hwan;Kim, Jae-Hoon;Ha, Hae-Chan
    • Journal of the Korean Arthroscopy Society
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    • v.10 no.2
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    • pp.214-218
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    • 2006
  • Purpose: We describe a new technique of arthroscopic medial plication using pull-out suture with consideration of anatomical location of medial patellofemoral ligament for the treatment of acute or recurrent patellar dislocation. Operative technique: Under arthroscopic examination, sutures are passed through the medial capsule, at which medial patellofemoral ligament is located, from outside to inside of knee joint. Three guide wires are inserted from anterior surface of the patella to upper half of its medial border. Intraarticular portions of sutures are pulled out toward anterior surface of the patella through bony tunnels. Under appropriate tension, the sutures are tied after performing lateral retinacular release. Conclusion: As suturing medial patellofemoral ligament, this technique can maximize the effect of medial plication and can correct subluxation and tilt of the patella. It seems to be a minimally invasive, easy and effective method for the treatment of acute or recurrent patellar dislocation.

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