• 한국가축번식학회지
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• 제24권2호
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• pp.189-197
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• 2000

본 연구는 골다공증유발 rat에 녹용추출물의 투여가 예방 및 치료효과에 미치는 영향을 구명하고자, 양측 난소를 적출한 후 골다공증이 유발된 rat에 암, 수녹용 추출물 525, 1,250 mg/kg을 투여했을 때 체중, 대퇴골의 중량, 장기중량, 골회분량의 측정 및 조직학적, 전자현미경적 검사 등을 실시하였다. 1. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 체중은 정상대조군에 비하여 유의한 증가를 나타냈다 (p＜0.05). 2. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 우측, 좌측의 femur 중량은 정상대조군에 비하여 감소하였다. 3. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 골회분량은 정상대조군에 비하여 약간 감소된 치를 나타냈으나 유의성은 인정되지 않았다. 4. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 간과 비장 및 신장의 각각 정상대조군에 비하여 감소된 치를 나타냈다. 5. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 조직학적 및 전자현미경적 소견은 정상대조군에서는 소주골간의 연결이 비교적 잘 유지되어 있고 골소실을 발견할 수 없었으나 난소적출군은 정상군에 비하여 소주골이 가늘어졌거나 소주골간 연결부분의 대다수가 끊어져 골양이 많이 소실되었음이 관찰되었으며, 녹용처리군에서는 소주골의 형태가 굵고 연결부분이 잘 유지되어 정상군과 유사한 소견이 관찰되었다. 위의 결과들에서 암, 수녹용 추출액은 난소제거후의 골소설을 억제하므로 골다공증의 예방과 치료에 효과가 있는 것으로 판단된다.

• 한국수산과학회지
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• 제36권2호
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• pp.80-87
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• 2003

수산가공부산물인 갑오징어갑과 수산가공 폐수 유래 수용성 단백질의 효율적 이용을 위한 일련의 연구로 유기산 처리 갑오징어갑 칼슘제 (이하 칼슘제)를 이용한 surimi의 가공폐수로부터 고분자 수용성 단백질의 분리를 시도하였고, 아울러 분리된 고분자 수용성단백질의 특성에 대하여 살펴보았다. 총질소, pH, COD, 탁도 및 수율의 결과로 미루어 보아 surimi의 가공폐수로부터 수용성 단백질의 회수를 위한 칼슘제의 최적 처리농도는 $1.0\%$로 판단되었다. SDS-PACE의 결과 칼슘제 처리에 의해 대부분의 고분자화합물 만이 회수되었고, 저분자 화합물의 잔존으로 COD는 높아 칼슘제 처리에 의한 폐수처리 효과는 미미하여 열처리 등의 병행처리가 있어야 하리라 판단되었다. 회수 단백질의 일반성분은 수분이 $78.4\%,$ 단백질이 $14.1\%,$ 지방이 $1.0\%,$ 회분이 $5.7\%$를 나타내었고, 시판 surimi와 비교하였을 때 회분이 높았으나, 기타 성분의 경우 거의 차이가 없었다, 또한 회수 단백질의 백석도는 시판 surimi보다 높았으며, 구성아미노산의 조성도 유사하였다. 시판 surimi에 대하여 회수 단백질을 $5\%$ 대체하여 어묵을 제조한 결과 관능적으로 백색도 및 조직감에서 차이가 인지되지 않았다. 이상의 결과로 미루어 보아 칼슘제 처리에 의해 수산가 공폐수로부터 회수한 단백질의 경우 어묵의 증량제로서 사용이 가능하리라 판단되었다.

• 한국수산과학회지
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• 제35권6호
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• pp.601-607
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• 2002

체내 칼슘 흡수 및 혈액 조성에 미치는 영향을 알아보기 위해 3주령 암컷 횐쥐 (Sprague-Dawley female raD)에게 다시마 첨가량을 각각 $0\%$, $0.5\%$, $1.0\%$, $1.5\%$로한 실험 사료를 8주 동안 사육하면서, 4주 간격으로 골격성장, 칼슘흡수, 혈액조성 및 분변에 관한 영향을 알아보았다. 사육기간 동안 사료 섭취량이 대조군과 다시마 첨가군 사이에 유의적인 차이가 없는 것으로 나타나 횐쥐가 다시마에 대한 거부반웅을 보이지 않았으며, 그로 인해 혈청 분석 결과 영양상태가 양호하였고, GPT, G07 활성 결과 독성에 의한 부작용도 없었다. 대조군에 비해 다시마 첨가군에서 평균 체중 증가량이 낮고 변 중량이 높아, 다시마 식이 섬유에 의한 배변 증량 효과를 보였다. 대퇴골의 분석에서 중량과 길이는 사육기간 동안 약간의 증가를 보였으며, 대퇴골의 수분함량이 감소한 반면 회분 및 칼슘함량은 증가하여 성장 동안 골밀도의 상승으로 인한 대퇴골의 강도가 높아짐을 보였다. 대조군에 비해 다시마 첨가군에서 칼슘함량과 대퇴골의 강도가 높음을 보석 다시마가 칼슘을 뼈로 홉착을 도와준고 있음을 설명해 주었다. 다시마 첨가수준에 따라서는 $0.5\%$ 첨가군에서 대퇴골의 수분함량이 낮고, 회분 및 칼슘함량이 높아 대퇴골의 강도가 가장 단단하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.396-403
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• 2016

The aim was to determine the relative bioavailability of phosphorus (P) in peas for 21-day old broiler chickens using slope-ratio assay. One hundred and sixty eight male Ross 308 broiler chicks were divided into 42 groups 4 balanced for body weight and fed 7 diets in a completely randomized design (6 groups/diet) from day 1 to 21 of age. The diets were a corn-soybean meal basal diet, and the corn-soybean meal basal diet to which monosodium phosphate, brown- or yellow-seeded pea was added at the expense of cornstarch to supply 0.5% or 1% total phosphorus. Monosodium phosphate was included as a reference, and hence the estimated bioavailability of P in pea cultivars was relative to that in the monosodium phosphate. Birds and feed were weighed weekly and on d 21 they were killed to obtain tibia. The brown-seeded pea contained 23.4% crude protein, 0.47% P, whereas the yellow-seeded pea contained 24.3% crude protein and 0.38% P. Increasing dietary P supply improved (p<0.05) chick body weight gain and tibia ash and bone density. The estimated relative bioavailability of p values for brown- and yellow-seeded peas obtained using final body weight, average daily gain, tibia ash, and bone mineral density were 31.5% and 36.2%, 35.6% and 37.3%, 23.0% and 5.60%, and 40.3% and 30.3%, respectively. The estimated relative bioavailability of p values for brown- and yellow-seeded peas did not differ within each of the response criteria measured in this study. In conclusion, the relative bioavailability of P in pea did not differ depending on the cultivar (brown- vs yellow-seed). However, the relative bioavailability of P in pea may vary depending on the response criterion used to measure the bioavailability.

• Journal of Acupuncture Research
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• 제26권2호
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• pp.1-14
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• 2009

Objectives & Methods : The purpose of this study is to observe the effects of herbal-acupuncture with Epimedii Herba extract (EH-HA) at $KI_{10}$ (Eumgok) on osteoporosis in ovariectomized (OVX) ddY mice. We carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results: 1. The tibial length and ash bone weight were insignificant, while BMD was significantly increased by in EH-HA in comparison with control group. 2. Serum ALP and serum osteocalcin were decreased and serum Ca was increased by in EH-HA in comparison with control group, But all were insignificant. 3. Ca and P level in tibia were significantly increased in EH-HA in comparison with control group, while Ca/P ratio was insignificant. 4. Osteoclast like cell in tibia was significantly decreased in EH-HA in comparison with control group. 5. In the histological study in tibia, TBV was significantly increased, TBT was insignificantly increased and GPL was significantly decreased in EH-HA in comparison with control group. Conclusions: These results suggest that EH-HA at $KI_{10}$ has a therapeutic effect on osteoporosis in OVX mice.

• 한국수산과학회지
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• 제50권5호
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• pp.473-480
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• 2017

We investigated the nutritional characteristics of the uncooked Skipjack tuna Katsuwonus pelamis frame (U-STF) as a basic resource for preparing flavor-enriching concentrates. The bone rate based on the fish frame (FF) was 53.2% in U-STF, thus lower than those of cooked skipjack tuna frame (C-STF), the uncooked salmon frame (U-SF), and beef leg bone (BLB). The composition of FF had 48.6-58.0% moisture, 18.4-21.3% crude protein, 7.0-15.0% crude lipid, and 12.8-22.1% ash. Compared to the proximate composition of BLB, that of FF was higher moisture and crude protein but lower solids, crude lipids, and ash. The total amino acid contents of FF was 17.6-20.8 g/100 g, which was higher than that of BLB. The levels of calcium and phosphorus of FF were 4.7-8.5 g/100 g and 2.4-4.2 g/100 g, respectively, thus lower than those of BLB. The brix concentration of FF extract was $5.0-8.2^{\circ}$, being highest in U-STF extracts, followed by extracts of U-SF and then extracts of C-STF. Our results indicate that U-STF is an optimal resource for preparing flavor-enriching concentrates.

• 한국가금학회지
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• 제27권3호
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• pp.243-254
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• 2000

The effect of supplemental microbial phytase and non - phytate phosphorus(NPP) levels on layer productivity and nutrient digestibility were conducted in 640 21 weeks - old HyLine brown layer for 12 weeks. Supplemented phytase levels were 0, 300, 500 and 1,000 DPU/kg diet. NPP levels were adjusted with tricalcium phosphate(TCP), which were 0(0.11% NPP), 0.5(0.20), 1.0(0.29) and 1.5%(0.38). ME, CP and Ca levels were maintained at 2,800㎉/kg diet, 16% and 3.5%, respectively. Egg production was increased with phytase compared to without phytase(P〈0.05). Increasement of egg production was higher latter of experimental period. Egg production was not different to phytase levels. Egg production in TCP levels were increased in above 0.5% compared to 0% TCP. Difference of egg production by TCP was higher after 6 week. Especially, egg production to supplemental phytase was higher in 0% TCP. Egg weight was not different to phytase and TCP levels. Egg mass was increased with phytase compared to without phytase, but not difference significantly. There was similar to phytase levels. Egg mass in TCP group was increased in TCP supplementation(P〈0.05). Feed intake was not different in phytase levels, and greater with increasing TCP levels(P〈0.05). Feed conversion was improved with phytase(P〈0.05), and not difference in TCP levels. All of nutrients digestibility tended to improve with phytase, P(P〈0.05), especially. There were not different among phytase levels. The effect of adding phytase was higher in low phosphorus diets compared normal levels. Eggshell breaking strength and eggshell thickness also improved in added phytase(P〈0.05). Tibial ash and P content were slightly increased with phytase, and Ca content also was higher(P〈0.05) compared without phytase. We concluded that supplemental phytase in low phosphorus diet was showed to increase laying performance, feed efficiency, nutrients digestibility, egg quality, and bone development. Phytase supplementation was able to compensate for low NPP diet. We also thought optimum phytase level is 300 DPU, and can decrease NPP supplementation adding phytase in later diet.

• 한국가금학회지
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• 제17권4호
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• pp.296-302
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• 1990

Aflatoxin $B_1$($AFB_1$ 0 또는 1ppm)과 Vitamin $D_3$($VD_3$0, 500, 1,000 또는 1,500IU/kg)의 교호작용을 조사코자, Hubbard계통 broiler 병아리 336수를 이용, 2$\times$4 요인시험이 실시되었으며, 전체8처리가 3반복으로 설계되어 탈지건조 중족골의 회분 및 무기물함량과 중량 등을 측정하였다. 얻어진 결과를 요약하면 다음과 같다. L 중족골의 신선중량에 있어서 $AFB_1$ 급여에 의한 차이는 나타내지 않았으며 $VD_3$의 수준이 증가됨에 따라 감소되는 경향을 보였다(P＜.01). 2. 탈지건조 중족골의 회분함양을 보면 $AFB_1$의 급여로 인하여 감소되었으며(P＜.01), $VD_3$수준이 증가될 때 반대로 증가되었다(P＜.01). 교호효과는 인정되지 않았으나 $AFB_1$ 1ppm 급여로 인한 회분감소를 극복하기 위하여 $VD_3$ 1,500IU/kg의 급여가 필요하다는 것이 증명되었다. 3. Ca의 함양은 AFB$_1$급여의 영향을 받지 않았으나 $VD_3$에 의하여 증가되는 경향을 보였다(P＜0.05). 4. P의 함양은 $AFB_1$급여로 감소되었으나(P＜.01) $VD_3$의 영향은 없었다. 5. Cu의 함양은 AFB$_1$$AFB_1$에 의하여 감소되었다(P＜.05). 6. Na Mg, K, Zn, Fe 및 Mn의 함량은 $AFB_1$이나 $VD_3$에 의하여 영향을 받지 않았다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권2호
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• pp.248-255
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• 2012

Three levels of boron (0, 30, 60 ppm) were supplemented in practical corn-soybean based starter and grower diets, containing either adequate or inadequate Ca or P. A total of 1,800, 1-day-old sexed broiler chicks were assigned to six dietary treatments and fed with the experimental diets for 42 days. Boron improved the overall feed conversion ratio, but increased body weight only at 21 days of age (p<0.01). Boron decreased feed intake in the case of feeding on a diet deficient in Ca and P, and tended to increase feed intake when birds received a diet adequate in Ca and P, signifying significant boron by Ca-P interaction (p<0.01). Mortality was not influenced by boron (p>0.05). Dietary Ca and P deprivation reduced body weight and feed consumption significantly, but did not influence the feed conversion ratio and mortality (p>0.05). Serum Ca level, ALP and ALT activities were not influenced either by dietary Ca and P deficiency or boron supplementation. Serum P content increased with respect to boron at 30 ppm. Bone breakage strength was not affected by dietary variables. Tibia ash, Ca and P were increased in response to the supplementation diet with 30 ppm boron, whereas 60 ppm showed no effect in most cases. Accordingly, the dietary boron supplementation of 30 ppm significantly decreased fecal Ca and P excretion, while there was a numerical decline in the 60 ppm boron as compared to the 0 ppm boron group. Data presented herein indicated that boron, either at the 30 ppm or 60 ppm supplementation level, was effective in conversion of feed to body weight, whereas only boron at 30 ppm contributed to the mineralization of bone thereby augmenting more Ca and P while excreting less through faeces.

• 동아시아식생활학회지
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• 제27권5호
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• pp.500-511
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• 2017

This study was conducted to investigate the anthropometric data, bone density, serum profiles, nutrient intakes and diet quality of premenopausal and postmenopausal women. For the study, we obtained data for analysis from the combined 2008~2011 Korean National Health and Nutrition Examination Survey (KNHANES). The height and weight were 157.8 cm and 58.7 kg in premenopausal women and 155.5 cm and 58.3 kg in postmenopausal women, respectively. The obesity rate was 27.49% in premenopausal women and 34.98% in postmenopausal women (p<0.001). Total cholesterol, LDL-cholesterol, triglyceride, GOT, GPT and alkaline phosphatase in postmenopausal women were significantly higher than those in premenopausal women (p<0.001). The prevalence of osteoporosis was 0.0~0.89% in premenopausal women and 0.48~13.22% in postmenopausal women (p<0.001). In postmenopausal women, rates of hypertension, stroke, myocardial infarction, depression, and diabetes were significantly higher than those in premenopausal women. Water, fat. ash, sodium, retinol, thiamin, riboflavin and niacin intakes in premenopausal women were significantly higher than those in postmenopausal women. Water, fiber, Ca, and, K intakes were below KDRIs (Dietary Reference Intakes for Koreans) in both groups. The mean adequacy ratio (MAR) of premenopausal women was higher than that of postmenopausal women (p<0.001). The index of nutritional quality (INQ) in premenopausal women was also higher than that of postmenopausal women except iron and vitamin C. Therefore dietary guidelines and an education program should be developed for desirable improvement of health, bone density, nutrient status and dietary quality of postmenopausal women.