• 대한치과보철학회지
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• 제22권1호
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• pp.33-50
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• 1984

The purpose of this study was to investigate whether the ashed tooth powder is utilized as an alternative material of the implant to recovery the bony defect. For this purpose its biocompatibility was evaluated comparing to the synthetic calcium phosphate compounds, such as Syntograft and Calcitite, as well as the vacuum firing porcelain (Ceramco Inc.) which is anticipated to use as a matrix to aid sintering. Bony defects to exposure the bone marrow, $3{\times}5$ mm in size, were created in the right and left tibias of fifteen rabbits, and then the ashed tooth powder at $950^{\circ}C$, the porcelain powder, Syhtograft and Calcitite were inserted in the defects of twelve rabbits of the experimental group and the blood clot only was filled in the defects of three rabbits of the control group. The experimental and control rabbits were sacrificed at 1st, 2nd 3rd week after implantation and the histologic examination was performed. The ashed tooth powder in order to make the needed form of the implant was molded using the cylindrical mold 1 cm high, 1 cm in diameter under the pressure of $1000kg/cm^2$ and the ashed tooth powder was sintered at $1100^{\circ}C$ for 1 hour and the mixture of the porcelain powder and the ashed tooth powder at the weight ratio of 7:3, 6:4, 5:5, 4:6 were molded in the same manner and were sintered at $925^{\circ}C$. From this sintered material, square shaped implants were prepared in the dimension of $2{\times}4{\times}6mm$. The prepared implants were surgically placed in the subperiosteum of lateral surfaces of the right and left mandibular bodies. The dogs were sacrificed at 4 weeks, and then the specimens were examined using the light and scanning electron microscopes. The results of this study were obtained as follows: 1. Any inflammatory response was not noted after implanting of the ashed tooth powder, Syntograft, Calcitite and the porcelain powder during the whole experimental period after implantation. 2. Induction of the new bone formation was significantly shown in the ashed tooth powder, Syntograft and Calcitite. 3. The more the porcelain powder was contained in the implants, the more the porosity was and the bigger the pore size was under the scanning electron microscope. And there was ingrowing of the fibrous connective and the osteoid tissue. 4. The osteoid tissues were found to be directly fused to the implant of the ashed tooth powder, and the mixture implant of the porcelain powder and the ashed tooth powder at the weight ratio of 4:6 under the light and scanning electron microscopes.

• Applied Biological Chemistry
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• 제46권2호
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• pp.101-106
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• 2003

수산가공 부산물인 갑오징어갑의 효율적 이용을 목적으로 아세트산 처리 갑오징어갑 분말(이하 갑오징어갑 북말)을 이용한 일반 두부의 제조를 시도하였고, 아울러 이의 특성에 대하여 살펴보았다. 두유의 응고능 실험에서 대두 단백질의 응고는 염화칼슘 0.7% 이상, 염화마그네슘 1.2% 이상 및 갑오징어갑 분말 0.9% 이상 첨가하는 경우 진행되었다. 따라서, 갑오징어갑 분말은 우수한 두부 응고제로 판단되었다. 수율, 백색도, 조직감 및 관능검사의 결과로 미루어 보아 갑오징어갑 분말로 두부를 제조하고자 하는 경우 최적 첨가비율은 2.0%로 판단되었다. 갑오징어갑 분말을 응고제로 하여 최적조건에서 제조한 두부의 일반성분으로는 수분이 75.9%, 조단백질이 12.6%, 조회분이 1.9% 및 조지방이 5.3%이었으며 pH는 6.60, 생균수는 $1.0{\times}10^4\;CFU/g$으로 식품위생법규에서 규제하고 있는 이미 및 이취(없어야 함), 고형물량(건물당 12% 이상), 회분(건물당 7% 이하)및 조단백질(건물당 40% 이상)함량에 대한 것을 모두 충족하였다. 또한 이 두부는 염화칼슘으로 제조한 두부에 비하여 색조 및 칼슘과 같은 기능성 무기성분의 함량은 유사하였고, 조직감은 부드러워 소비자의 기호를 충족시킬 수 있는 제품으로 판단되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1348-1352
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• 2005

Most breeds of sheep in Iran are adapted to their agro ecological niches where it is likely that they were also artificially selected by their owners. In general, most of sheep breeds are multipurpose producing lambs, wool and milk. To compare the physical and chemical composition of the carcasses of ten Iranian native fat-tailed sheep breeds, 243 male lambs (6-7 months of age) of ten fattailed, Iranian breeds of sheep, Sanjabi (S), Ghezel (G), Afshari (A), Mehrabani (M), Lori (L), Lori Bakhtiari (LB), Kordi Khorasan (K), Sangesari (Sa), Baluchi (B) and Chal (C) were studied. Lamb breed group had a significant (p<0.05) effect on all the carcass traits measured. The LB and C lambs showed the same live weight which was significantly (p<0.05) higher than the other breeds. Carcass dressing- out percentage in S lambs was lowest (p<0.05), but not different from G lambs. K and Sa breeds showed the highest (p<0.05) carcass dressing-out percentage. The S lambs had the highest (p<0.05) lean meat percent. Lean meat percentage was not significantly (p>0.05) different in the G, A, M and C breeds. The Sa and K breeds showed the lowest lean meat percent. The S lambs showed the lowest (p<0.05) fat percent in their carcass, while K and Sa showed the highest (p<0.05). Subcutaneous fat in K, Sa and B was higher (p<0.05) than the other breeds. Lambs of S, G, A and M breeds had the lowest subcutaneous fat in their carcasses (p>0.05). Intramascular fat was significantly (p<0.05) lower in M, S and C despite the fact that this values were highest in B and K lambs. The K and Sa breeds had highest fat-tail percentage (p<0.05) in their carcass, whereas S and G showed lowest. Lambs of G, S and A breeds had higher bone percent than other breeds (p<0.05). Lowest bone percent (p<0.05) was seen in K and Sa lambs. The carcass moisture percent was not significantly (p>0.05) different in S, G, A, M, L and C breeds. M lambs showed the lowest crude protein percentage and S breed the highest (p<0.05). There were no significant (p>0.05) differences among other lamb breeds for this trait. Chemical fat percentage was the same in S, G, A, C and M breeds, but significantly (p<0.05) lower from LB, K, Sa and B. Ash percent in S, G and A had no significant (p>0.05) difference. According to higher lean meat and lower fat percentages in the carcass, the ranking of breeds would be S, G, A, M and C.

• Preventive Nutrition and Food Science
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• 제9권1호
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• pp.45-52
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• 2004

Effects of dietary calcium lactate (CaL-A) and Chungkukjang (Korean native fermented soybean) on bone mass, calcium status, body weight, serum glucose and cholesterol levels in young male rats were investigated. Chungkukjang was fermented by mixing 4 types of Bacillus sp., and then dried at 45$^{\circ}C$. Calcium lactate was prepared from the ash of black snail. The rats were fed a commercial rat diet for 1 week and then the experimental diets for 4 weeks. Animals were divided into four dietary groups: one calcium-deficient diet (Ca-De) and one of three with calcium supplemented diets (5 g calcium/kg diet) with either calcium phosphate (Ca-P), CaL-A, or CaL-A + Chuntkukjang (CaL-AC). Calcium supplemented diets contained 39 g Ca-P/kg diet and 28 g/kg of calcium lactate in the CaL-A and CaL-AC diets. Body weight gains during the 4 weeks in the Ca-P, CaL-A, CaL-AC and Ca-De groups were 130.45 g,112.50 g, 143.40 g and 10.20 g, respectively. Feed consumption of the groups from high to low was CaL-AC > Ca-P > CaL-A > Ca-De. The Ca-De group had low femur weights and low serum calcium concentrations, while they were comparatively high in CaL-AC, Ca-P and CaL-A groups. The Ca-De groups excreted less calcium in urine than did the other rats, probably due to increased absorption of the mineral in Ca-P, CaL-A and CaL-AC groups. Microscopic observations revealed that there were many regularly spaced holes in the femur of Ca-De group, while there were much smaller regularly spaced holes in Ca-P group. However, no holes in femur were observed in the CaL-A and CaL-AC groups. Bone surfaces were especially smooth and clean in the CaL-AC group. Serum concentrations of glucose and total cholesterol were remarkably lower in the CaL-AC group than in the other supplemented groups. These results suggest that calcium from CaL-A has higher bioavailability than from Ca-P, and dietary Chungkukjang may have a beneficial effect on calcium metabolism.

• Journal of Animal Science and Technology
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• 제65권2호
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• pp.336-350
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• 2023

Two experiments were conducted in this research. Experiment 1 investigated the spatial expression characteristics of calcium (Ca) and phosphorus (P) transporters in the duodenum, jejunum, and ileum of 21-day-old broilers provided with adequate nutrient feed. Experiment 2 evaluated the effects of dietary vitamin D₃ (VD₃) concentration (0, 125, 250, 500, 1,000, and 2,000 IU/kg) on growth performance, bone development, and gene expression levels of intestinal Ca and P transporters in 1-21-day-old broilers provided with the negative control diet without supplemental VD₃. Results in experiment 1 showed that the mRNA levels of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), plasma membrane calcium ATPase 1b (PMCA1b), and IIb sodium-phosphate cotransporter (NaPi-IIb) were the highest in the broiler duodenum. By contrast, the mRNA levels of inorganic phosphate transporter 1 (PiT-1) and 2 (PiT-2) were the highest in the ileum. Results in experiment 2 showed that adding 125 IU/kg VD₃ increased body weight gain (BWG), feed intake (FI), bone weight, and percentage and weight of Ca and P in the tibia and femur of 1-21-day-old broilers compared with the negative control diet (p < 0.05). The rise in dietary VD₃ levels from 125 to 1,000 IU/kg further increased the BWG, FI, and weights of the bone, ash, Ca, and P (p < 0.05). No difference in growth rate and leg bone quality was noted in the broilers provided with 1,000 and 2,000 IU/kg VD₃ (p > 0.05). Supplementation with 125-2,000 IU/kg VD₃ increased the mRNA abundances of intestinal Ca and P transporters to varying degrees. The mRNA level of CaBP-D28k increased by 536, 1,161, and 28 folds in the duodenum, jejunum, and ileum, respectively, after adding 1,000 IU/kg VD₃. The mRNA levels of other Ca and P transporters (PMCA1b, NCX1, NaPi-IIb, PiT-1, and PiT-2) increased by 0.57-1.74 folds by adding 1,000-2,000 IU/kg VD₃. These data suggest that intestinal Ca and P transporters are mainly expressed in the duodenum of broilers. Moreover, the addition of VD₃ stimulates the two mineral transporter transcription in broiler intestines.

• Asian-Australasian Journal of Animal Sciences
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• 제13권6호
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• pp.824-829
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• 2000

A five wk feeding experiment was conducted with day-old one thousand broiler chicks (Arbor Acres) to determine the effects of microbial phytase (Natuphos$^{(R)}$) supplemented to low nonphytate P (NPP) corn-soy diets. Five pens of 50 mixed sex birds each were randomly assigned to each of the four dietary treatments: T1, control diet containing normal NPP level; T2, T1-0.1% NPP+600 U of phytase/kg diet; T3, T1 - 0.2% NPP+600 U of phytase/kg diet; and T4, T1 - 0.3% NPP+600 U of phytase/kg diet. T1, T2, and T3 showed similar growth rate, feed intake, and feed efficiency, indicating that NPP level in broiler diets could be reduced by approximately 0.2% by the microbial phytase supplementation. But T4 showed significantly (p<0.05) lower weight gain than others. The phytase supplementation improved P availability resulting in low P excretion. Weight and girth of metatarsal bone were increased by phytase supplementation at low NPP diet treatments but ash contents were not significantly different. It can be concluded that NPP level of corn-soy broiler diets can be safely lowered by approximately 0.2 % by supplementing 600 U of microbial phytase/kg diet. With the adjusted level of NPP and phytase supplementation, P excretion could be reduced by 50%.

• 동아시아식생활학회지
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• 제13권3호
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• pp.176-184
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• 2003

This experiment was planned to develop a functional supplements by food resources to prevent and lessen the bad effects caused by the environmental pollution such as bad food, air, water and heavy metals exposed to people these days. As for Its primary stage, the nutrient profile and sensory characteristics of the duck-extract were evaluated in this study. The duck-extract was formulated by the mixture of bone and internal organs of ducks and 6 medicinal herbs which were able to be used as foods and known to help the excretion and the repression of the poison inside the body for a long time. As we compared the six medicinal herbs mentioned above to the vegetable herbs, the nutrient profile of the medicinal herbs were superior to the vegetable herbs. The duck-extract was composed of protein 49.92%, carbohydrate 37.02%, dietary fiber 20.99%, lipid 7.60% and ash 5.46%. The ratio of Ca to P was 1 : 1.4, which was a suitable ratio for absorption, the contents of Na and K were low and those of micro-elements such as Fe, Mn, Zn and Cu high compared to other meat extracts. The essential amino acids accounted for 30.91% of total amino acids. The result of sensory evaluation was better in overall preferences than the ones which are already in the market. From these results, the nutrient profile of the duck-extract was estimated to be able to supply enough nutrients to the people whose nutritional balance was destroyed these days. This study also showed the effective way of using duck-extract and its application to the oriental medicine.

• Journal of Nutrition and Health
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• 제26권2호
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• pp.145-155
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• 1993

A factorial experiment was conducted to determine the influence of phytate(0 or 10g/kg diet) and calcium (Ca)(3 or 10g/kg diet) intakes on Ca, P and Zn metabolism by growing female rats. Food intake and weight were similar for the all groups, however, phytate ingestion for six weeks depressed femur growth. The low Ca plus phytate group showed the lowest Ca content of total femur and this was related to a significant decrease of Ca retention. Phytate intake depressed zinc(Zn) absorption in the first metabolic collection. This inhibitory effect of phytate on Zn absorption was improved in the low Ca plus phytate group after several weeks. Impared Zn absorption however remained in the high Ca plus phytate group which was reflected in the lowest Zn content of femur, phytate intake with high Ca also depressed phosphorous(P) absorption and serum and urinary P. These adverse effects of phytate on Zn and P absorption when the dietary Ca was high could explain reduced femur weight despite the highest concentration of femur Ca(mg/g ash) in this group. Results suggest that phytate can adversely affect not only Ca metabolism but Zn and P utilization. Thus, for the normal bone growth when phytate intake is high, the ingesion of Ca, P, Zn and other minerals should be enhanced.

• 한국조리학회지
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• 제16권5호
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• pp.287-298
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• 2010

본 연구에서는 소스나 스프 등의 모체가 되는 닭 육수의 제조에 닭뼈를 이용하여 각종 영양 성분이 적절하게 이용되기 위한 닭 육수를 만들고자 했다. 닭 육수의 맛과 영양을 향상시키고 국물을 이용하는 요리의 품질 개선과 최상의 육수개발의 기초 자료로 이용하기 위하여 토마토를 이용한 닭 육수를 제조하였다. 따라서 토마토의 첨가량에 따른 수분, 회분, 색도, 당도, pH, 유리 아미노산, 관능적 특성 및 기호도 분석을 통하여 육수 제조 시 첨가되는 토마토에 함유된 유기산을 이용하여 닭뼈에서 용출되는 유리 아미노산과 각종 영양성분에 따른 관능적 특성을 알아 보고자 했다. 총 유리 아미노산의 함유량과 종합적인 기호도는 7.4%의 토마토를 첨가하였을 때 가장 높았다. 이상의 연구 결과를 통해 적절한 토마토의 첨가는 전체적인 기호도에 긍정적인 영향을 미치며, 닭 육수의 제조에 있어서 토마토 7.4% 첨가가 가장 적절한 것으로 평가되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제2권1호
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• pp.7-16
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• 1989

To determine the effect of sodium plus potassium to chloride ratio and lysine level on blood pH, blood acid-base parameters, lysine-arginine antagonism and growth performance, four hundred and thirty two chicks of 3 days age were used in a completely randomized $3{\times}3$ factorial experiment. Variables contained three levels of lysine (0.8, 1.2 and 1.6%) and dietary electrolyte (100, 200 and 300 mEq/kg). Birds fed 200 mEq/kg and electrolyte had the best growth rate and feed efficiency, followed by those fed 300 mEq/kg and 100 mEq/kg electrolyte. It is proposed that high levels of dietary electrolyte may improve the growth of chicks fed diets containing excess lysine by increasing lysine catabolism. High or low levels of lysine and dietary electrolyte resulted in higher mortality than those of optimum level (1.2%) of lysine and 200 mEq/kg of electrolyte balance. When the electrolyte level was increased, the pH, $pCO_2$, base excess, $HCO_3{^-}$ and total $CO_2$ of blood plasma were increased. The utilization of nutrients was changed when the electrolyte and lysine were manipulated. Plasma chloride tended to be greater in chicks receiving high chloride diet and was the highest in chicks fed the high lysine diet. Plasma sodium and potassium were unaffected by dietary lysine. Diet containing high lysine decreased the level of arginine and excess dietary electrolyte increased arginine level in plasma. It may be concluded that cation supplementation tended to alleviate the lysine-arginine antagonism but chloride exacerbated. Tibia bone length and ash contents were significantly affected by electrolyte balance and lysine level.