• Journal of Chest Surgery
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• v.25 no.8
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• pp.806-811
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• 1992

The primary neuroendocrine carcinomas[NEC] of the lung are relatively rare. There are classified histologically into the following groups. 1] bronchial carcinoid, 2] well differentiated neuroendocrine carcinoma[WDNC], 3] intermediate cell neuroendocrine carci-noma[ICNC], 4] small cell neuroendocrie carcinoma[SCNC]. The NE cells can be found throughout the mucosa of the bronchial tree by the electron microscopy. By the immunocytochemistry, Neuron-specific enolase[NSE], serotonin, born-csin, and calcitonin are found in normal solitary NE cells and neuroepithelial bodies. The immunohistochemistry has the advantage that it can be applied on routine pathology specimens. The NSE was localized in all of the NE granule bearing tumors but also present in 57 per cent of the non NE carcinomas. Together the combination of chromogranin A, bombesin and NSE should provide an exellent signature for tumor cells expressing NE features, such as carcinoids and other NE carcinomas. We have experienced two cases of primary pulmonary NEC in 59 and 29 years old men. The former was admitted due to exertional dyspnea and left chest pain, the latter, newly developed mass on chest X-ray. They were treated by Lt pneumonectomy with LN dissection and RLL resection with LN dissection The postoperative diagnoses were could be confirmed by NSE study as NEC which were SCNEC and INEC in subgroups. The postoperative courses were uneventful.

• Korean Journal of Veterinary Research
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• v.39 no.4
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• pp.689-697
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• 1999

The regional distribution and relative frequency of endocrine cells in the alimentary tract of the snake, Rhabdophis tigrinus tigrinus, were investigated by immunohistochemical method using 7 antisera. Chromogranin (Cg)-, glucagon-, somatostatin-, gastrin/cholecystokinin (Gas/CCK)-, serotonin-, bovine pancreatic polypeptide (BPP)-immunoreactive cells were identified in this study. Cg-immunoreactive cells were detected throughout the alimentary tract including the esophagus, with predominant frequency in the pylorus. Numerous immunoreactive cells were observed from the esophagus to the pylorus but a few cells were detected in the large intestine. Glucagon-immunoreactive cells were observed from the proximal portions to the distal portions of the small intestine. They were increased to the middle portions but thereafter decreased, and no cells were found in the terminal portions. Somatostatin-immunoreactive cells were restricted to the small intestine and these cells were decreased toward to distal portions of the small intestine. Gas/CCK-immunoreactive cells were detected in the pylorus and small intestine. They were most predominant in the pylorus and the proximal portions of the small intestine but thereafter decreased toward to the distal regions. Serotonin-immunoreactive cells were observed throughout the alimentary tract. They were most predominant in the pylorus and proximal portions of the small intestine but a few cells were observed in the large intestine. BPP-immunoreactive cells were restricted to the distal portions of the small intestine with rare frequency. No bombesin-immunoreactive cells were found in this study.

• Applied Microscopy
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• v.25 no.1
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• pp.48-64
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• 1995

Ultrastructure of nerves and their associated cells in the bronchiolar epithelium of the human fetal lung were studied with ultrastructural and immunohistochemical methods. The neuroendocrine cells were scattered along the basal part of non-ciliated respiratory epithelium and appeared as single cell (solitary neuroendocrine cell) or groups (neuroepithelial bodies). The solitary neuroendocrine cells were devoid of any detectable innervation, while the neuroepithelial bodies were associated with nerve ending containing morphologically afferent (sensory) and efferent (motor) intraepithelial terminals. The afferent nerve endings contained abundant mitochondria with long cristae. The efferent nerve endings were characterized by the presence of synaptic vesicles. Both types of nerve endings formed synaptic junction between nerve endings and neuroepithelial bodies cells. Serial sections of the intraepithelial nerves revealed that both morphologically afferent and efferent types of nerve endings may be formed by the same nerve fiber. By immunohistochemistry, bombesin and serotonin were localized in solitary neuroendocrine cells and neuroepithelial bodies of human fetal lung from various prenatal age groups. These results suggest that the neuroepithelial bodies cells of the human fetal lung have neuroreceptor function.

• Korean Journal of Veterinary Research
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• v.32 no.3
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• pp.321-331
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• 1992

The regional distribution and relative frequency of gastrointestinal endocrine cells were studied immunohistochemically in the gastrointestinal mucosa and pancreas of the fresh water turtle. Ten kinds of endocrine cells were identified in the gastrointestinal tract. Cholecystokinin-8-, bovine pancreatic polypetide-and glucagon-immunoreactive cells were seen throughout the gastrointestinal tract, also among them cholecystokinin-8-immunoreactive cells were most predominant in segment III. Although gastrin- and gastrin/cholecystokinin-immunoreactive cells were found from segment III to VI and X, respectively, they were numerous in segment III. Somatostatin-immunoreactive cells were observed from segment I to VII. 5-hydroxytryptamine- immunoreactive cells were detected in segment I, III, VIII, IX and X. Human pancreatic polypeptide-immunoreactive cells were demonstrated in segment V, VI, VIII, IX and X. Insulin-immunoreactive cells were found from segment III to X except for segment VIII, but rare in segment VII. Neurotensin-immunoreactive cells were found to be restricted to segment VIII, IX and X. No porcine chromogranin-, substance P- and bombesin-immunoreactive cells were detected throughout the gastrointestinal tract of the fresh water turtle. Although typical mammalian pancreatic islets encapsulated by connective tissue were not present in this species, five kinds of endocrine cells-glucagon, insulin, somatostatin, bovine pancreatic polypeptide and 5-hydroxytryptamine-were found in forming small or large groups and scattered in the exocrine gland region. However porcine chromogranin- and motilin-immunoreactive cells could not be demonstrated in the pancreas.

• The Korean Journal of Zoology
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• v.33 no.1
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• pp.22-27
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• 1990

The gastro-endocrine cells were examined in the fundic region of stomach of the frog, Rana rugosa, by transmission electron microscope. In the present paper, at least three kinds of cell type distributed in this region were idendfted according to their morphological charactedstics based on the size, shape and electron density of the secretory granules. Type I cells were characterized by the presence of round or oval granules (300-5OOnm in diameter) with high electron density. The granules showed a wide lucent between the contents and the limiting membrane. This cell was reminiscent of the ECL cell in the human alimentary mucosa. Type II cells were characterized by the presence of spherical or oval granules (11O-230nm in diameter) with low or high electron density. The granules showed a clear halo between the homogenous contents and the limiting membrane. This cell was reminiscent of the G cell in the human alimentary mucosa. Type Ill cells were characterized by the presence of elongated oval or pheomorphic granules (50-2OOnm in diameter) with low or moderate electron density and abundant microfilament bundles in the cytoplasm. The granules contained a very thin halo.

• The Korean Journal of Physiology
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• v.23 no.1
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• pp.89-98
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• 1989

In order to produce antibody for use in radioimmunoassay of gastrin in physiological concentration, four rabbits of New Zealand white were immunized with synthetic human gastrin-17-I conjugated to hemocyanin with EDC. Among them, only one rabbit produced antibody that could bind 50% of $^{125}I-gastrin$ at a final dilution of 1:25,000. $^{125}I-gastrin$ was prepared with synthetic human gastrin-17-I and $NaI^{125}$ by lactoperoxidase technique. The product was then purified on a column of Sephadex Gl5/G5O (7:3, w/w) followed by a column of DEAE sephadex A-25. The specific radioactivity of the purified $^{125}I-gastrin$ was in the range of 347-1429 ${\mu}Ci/nmole$ when determined by the self-displacement method. The effective affinity constant $(K_{eff})$, total binding sites (N), heterogeneity index $({\alpha})$ and average affinity constant $(K_{0})$ of the anti-gastrin serum calculated from Scatchard plot as well as Sips plot were $1.77{\times}10^{11}/M$, 255 nM, 0.84 and $0.79{\times}10^{11}/M$, respectively. When radioimmunoassay was performed with the anti-gastrin serum, it was confirmed that the mean concentration of gastrin immunoreactivity in plasma was increased by feeding in humans and rats, and also increased by bombesin administration in rats. The results indicate that the anti-gastrin serum produced in the present investigation is suitable for radioimmunological determination of gastrin in physiological concentration.