• Title/Summary/Keyword: bolus

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Protective Effect of Nicotine on Gastrin-induced Gastric Mucosal Damage in Rats (Gastrin 유발 위점막 손상에 대한 Nicotine의 보호 효과)

  • Piao, Shi-Hao;Kim, Dong-Goo;Jin, De-Nan;Wu, Zhen-Jiu;Hong, Chun-Lan;Kim, Kyung-Hwan
    • The Korean Journal of Pharmacology
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    • v.31 no.3
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    • pp.313-321
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    • 1995
  • Conflicting data have been reported on the effect of nicotine on gastric mucosal damage. To elucidate the effect of chronic intermittent nicotine on gastric mucosal damage, intragastric nicotine (5 mg/kg, 10 mg/kg) was administered twice per day for 9 days. Gastric mucosal damage was created by s.c. injection of a large dose (1.2 mg/kg) of pentagastrin followed by pylorus ligation for 6 hours. Nicotine treated rats showed reduced gastric mucosal damage about 50% of the control. To examine the mechanism of the protective effect of nicotine, gastric perfusion experiments were done. Basal acid secretion was not affected by intragastric or intravenous nicotine. However, pentagastrin-stimulated acid secretion markedly inhibited by a bolus injection of nicotine, and this response was dose-related. These data indicates that chronic intermittent administration of nicotine protects gastric mucosa against gastrin-induced gastric mucosal damage, and nicotine-induced inhibition of gastrin-stimulated acid secretion has an important role for the protective effect of nicotine. Considering reports concerning nicotine's aggravating effect on the gastric mucosal damage, it is suggested that the methods of administration of nicotine may be an important decisive factor of the divergent action of nicotine on the gastric mucosa.

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Near-Infrared Spectroscopy for Monitoring Cerebral Hemodynamics in Hyperbilirubinemia-induced Newborn Piglets (고빌리루빈혈증이 유도된 신생자돈에서 근적외석 발광기를 이용한 뇌 혈역학적 변화에 대한 연구)

  • Hwang, Jong Hee;Choi, Chang Won;Chang, Yun Sil;Park, Won Soon
    • Clinical and Experimental Pediatrics
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    • v.48 no.6
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    • pp.649-654
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    • 2005
  • Purpose : The present study examined how changes in cerebral hemodynamics in newborn piglets with bilirubin infusion can be evaluated by near infrared sepctroscopy(NIRS). Methods : Seventeen newborn piglets were randomly divided into the following three experimental groups : six in the control group(CG); seven in the bilirubin infusion group(BG), and four in the bilirubin infusion with 7-nitroindazole group(NG). To achieve the concentration of bilirubin above 20 mg/dL, we injected a bolus of 40 mg/kg of bilirubin intravenously, followed by 30 mg/kg/hr of bilirubin continuous intravenous infusion. All groups were monitored with cerebral hemodynamics using near infrared spectroscopy(NIRS) and their brain cortexes were harvested and the activities of $Na^+$, $K^+$-ATPase, level of conjugated dienes, ATP and phosphocreatine(PCr) were determined biochemically. Results : No changes took place in CG. In BG and NG, base excess, pH, and MABP decreased, and lactate level in blood increased. Cerebral $Na^+$, $K^+$-ATPase activity and ATP, PCr level in BG significantly decreased and conjugated dienes increased compared to CG. These abnormalities observed in the BG were significantly improved in the NG. In continuous NIRS monitoring, [$HbO_2$], [HbT], and [HbD] in BG were significantlly decreased compared to CG. However these abnormalities between NG and CG were not significantly different. There were no significant differences in $ScO_2$ between the study groups. Conclusion : Our study suggests cerebral hemodynamic changes could be monitored by non-invasive NIRS in newborn piglets with bilirubin infusion.

Deprivation of Esophageal Boluses and Dry Forage Intake in Large-type Goats

  • Van Thang, Tran;Sunagawa, Katsunori;Nagamine, Itsuki;Kato, Seiyu
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.9
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    • pp.1174-1183
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    • 2010
  • In goats fed on dry forage twice a day, an esophageal fistula was used to investigate the physiological factors present in the marked suppression of dry forage intake that occurs after 40 min of feeding. The animals used in this study were five large-type male esophageal- and ruminal-fistulated goats. Roughly crushed alfalfa hay cubes with any large remaining chunks removed were used as feed for this research. The study was conducted under both normal feeding conditions (NFC) and sham feeding conditions (SFC). In the NFC control, the esophageal fistulae were closed by plugs and the animals ate dry forage in the normal manner. In the SFC treatment, before starting the experiment the plugs for closing the esophageal fistula were removed and the cannulae for collecting boluses were fitted into the fistulae. Therefore, the esophageal boluses were removed via an esophageal fistula before they entered the rumen. In the NFC control, eating rates sharply decreased in the first 40 min of feeding and were subsequently maintained at low levels. However, eating rates in the SFC treatment remained high after 40 min of the feeding period had elapsed and the goats ate continuously during the 2 h feeding period. In comparison with the NFC control ($1,794{\pm}203.80\;g$/2 h), cumulative dry forage intake in the SFC treatment ($3,182{\pm}381.69\;g$/2 h) was 77.4% greater (p<0.05) upon conclusion of the 2 h feeding period. In the SFC treatment, cumulative bolus output ($6,804{\pm}469.92\;g$/2 h) was about twofold the cumulative dry forage intake due to cumulative salivary secretion volume ($3,622{\pm}104.13\;g$/2 h) upon conclusion of the 2 h feeding period. The result indicates that large amounts of secreted saliva during dry forage feeding act in conjunction with consumed feed to form the ruminal load responsible for ruminal distension. The increased plasma total protein concentrations were higher in the SFC treatment than in the NFC control. However, plasma and ruminal fluid osmolalities increased in the NFC control during and after feeding but were mostly unchanged in the SFC treatment. In comparison with the NFC control ($3,440{\pm}548.04\;g$/30 min), thirst level in the SFC treatment ($1,360{\pm}467.02\;g$/30 min) was 60.5% significantly less (p<0.05) upon conclusion of the 30 min drinking period. The results of the present study indicate that In the second hour of the 2 h feeding period, dry forage intake is regulated by factors produced when boluses enter the rumen.

Pharmacokinetics of Arsenic Hexaoxide, a Anticancer Compound, in Rats (흰쥐에서 항암성화합물인 육산화비소의 체내동태)

  • Lee, Pung-Sok;Shin, Dae-Hwan;Lee, So-Young;Lee, Jung-Yeol;Lee, Kyoung-Mi;Kwon, Koo-Hyun;Chung, Youn-Bok
    • Journal of Pharmaceutical Investigation
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    • v.36 no.6
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    • pp.377-383
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    • 2006
  • The purpose of the present study was to examine the pharmacokinetic characteristics of arsenic hexaoxide($As_4O_6$), a novel anticancer compound, after i.v. bolus and oral administration in rats. We developed an ICP-Mass based method to analyze arsenic hexaoxide levels in plasma, bile, urine, feces, and tissue and validated the method. Arsenic hexaoxide rapidly disappeared from the plasma by 10 min($\alpha$ phase) after i.v. administration, which was followed by the late disappearance in the $\beta$ phase. The mean plasma half-lives($t_{1/2}$) of arsenic hexaoxide at the a and $\beta$ phase when administered at a dose of 5 mg/kg were 1.57 and 29.8 min, respectively. The maximum plasma concentration($C_{max}$) was 230 ng/mL, after oral administration of arsenic hexaoxide at a dose of 50 mg/kg. The bioavailability, which was calculated from the dose-adjusted ratio, of the oral administered arsenic hexaoxide was 1.61%. Of the various tissues tested, arsenic hexaoxide was mainly distributed in the spleen, lung, liver and kidney after oral administration. Arsenic hexaoxide levels in the spleen or lung at 24 hr after oral administration were higher than those of maximum plasma concentration($C_{max}$). The cumulative amounts of arsenic hexaoxide found in the urine by 48 hr after the administration of 50 mg/kg were 5-fold higher than those in the bile. However, the cumulative amounts in the feces were 10-fold higher compared with those of urine, suggesting that arsenic hexaoxide is mostly excreted in the feces. In conclusion, our observations indicated that arsenic hexaoxide was poorly absorbed from the gastro-intestinal tract to the blood circulation and transferred to tissues such as the spleen and lung at 24 hr after oral administration. Moreover, the majority of arsenic hexaoxide appears to be excreted in the feces by 48 hr after oral administration.

두경부암의 6MV 광자선 치료 시 표면선량 증가를 위한 Spoiler의 유용성 평가

  • 이강혁;김원택;이화중;김대영
    • The Journal of Korean Society for Radiation Therapy
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    • v.14 no.1
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    • pp.41-47
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    • 2002
  • 1.목적 두경부암(head and neck Ca)과 쇄골상부(Supraclavicular)에 6MV 광자선으로 치료 시 치료부위(Target volume)가 피부에서 대략 $1^{\sim}2mm$정도 깊이에 위치할 경우, 6MV 광자선의 선량분포는 표면선량이 낮아서 치료에 적합하지 않기 때문에 Bolus와 같이 사용하지만 Skin Sparing(피부보호)효과의 손실로 피부의 손상이 발생할 수 있다. 이러한 이유로 피부의 보호와 치료 시 표면선량의 증가를 위해 Spoiler(산란판)를 제작하여 측정 후 그 특성을 이해하고 선량의 분포를 통하여 Bolus와 비교한 후에 Spoiler의 유용성에 대해 평가하고자 하였다. 2.방법 Siemens사 선형가속기(PRIMUS)의 6MV 광자선을 사용하여 Spoiler의 사용여부 및 Spoiler의 사용 시에는 조사면의 크기를 $5{\times}5,\;7{\times}7,\;10{\times}10,\;15{\times}15,\;20{\times}20cm^2$로 하고 Spoiler와 표면과의 거리는 6, 10, 15cm로 바꾸어 가면서 물팬톰(PTW. 독일)을 이용해 깊이와 측방에 따른 선량분포를 Markus 전리함(PTW. 독일)으로 측정하였으며 전리함의 방수를 위해 씌어진 방수 캡 때문에 표면선량을 별도의 고형 팬톰으로 측정하였다. 표면의 측정선량은 전리함의 측면 벽 등에 의한 선량 측정치의 증가 현상으로 과 반응을 보였으며 이를 교정하였다. 그리고 측정된 데이터를 치료계획 시스템(Pinnacle 6.0m)으로 비교, 분석하였다. 3.결과 Spoiler의 사용 시 3cm깊이 측정선량 백분율과 Spoiler를 사용하지 않은 해당 치료 조사면의 3cm깊이 선량의 백분율에 일치하도록 하여 가상의 치료 깊이인 2mm에서 측정값을 비교하여 본 결과 조사면 $5{\times}5,\;10{\times}10,\;20{\times}20cm^2$에서 OPEN시 62, 64, $70\%$, Bolus는 97, 97, $99\%$로 Spoiler의 사용 시 표면과의 거리가 6cm에서 82, 98, $103\%$, 10cm에는 72, 89, $101\%$, 15m에 65, 79, $96\%$로 나타났으며 표면에서의 측정값을 비교하여 본 결과 OPEN시 11, 17, $27\%$, Bolus는 84, 84, $86\%$, Spoiler의 사용 시 6cm에서 40, 71, $93\%$, 10cm에는 25, 50, $81\%$, 15cm에 18, 36, $67\%$를 나타내었다. 또한 3m깊이에서의 측방 선량분포에서 Spoiler의 거리변화(6, 10cm)는 심부선량의 변화에 영향을 주지 않는 것으로 확인할 수 있었다. 그리고 위의 실험측정치를 치료계획 시스템에 입력하여 선량분포를 확인한 결과 Spoiler를 사용하는 경우 OPEN에 비해 선량분포 영역을 표면으로 끌어 올릴 수 있으며 Bolus 보다 피부 보호효과는 어느 정도 유지가 되는 것을 보여주었다. 4.결론 이와 같이 Spoiler는 Bolus와 비교하여 6MV 광자선의 build up 영역을 표면으로 증가시키는 동시에 Skin Sparing(피부보호)효과를 유지할 수 있으며 두경부암의 치료에서 Spoiler의 사용이 가능한 조건으로는 조사면이 $5{\times}5cm^2$에서 Spoiler와 표면과의 거리가 6cm일 때, $7{\times}7cm^2$에서 6cm, 10cm였고 $10{\times}10cm^2$는 10cm, 15cm로, $15{\times}15cm^2$는 15cm의 간격으로 평가되었다. 또한 $20{\times}20cm^2$의 조사면, Spoiler가 6cm 간격 인 경우 Bolus를 사용한 것 보다 더욱 높은 표면선량을 나타내었다. 그러나 Spoiler와 표면간의 거리를 다르게 함으로써 깊이에 따라 선량분포를 다양하게 나타낼 수 있기 때문에 표면선량의 증가와 피부의 보호를 위해 환자의 피부 두께, 실제 치료 부위의 깊이 등을 고려한다면 Spoiler를 사용하는 것이 bolus를 사용하는 것보다 더 유용하게 적용할 수 있을 것으로 사료된다.

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Thiopental Prevents A Beta-Endorphin Response to Cardiopulmonary Bypass (체외순환전 투여된 Thiopental이 Beta-endorphin치 변화에 미치는 영향)

  • Song, Sun-Ok;Carr, Daniel B.;Park, Dae-Pal;Jee, Dae-Lim;Kim, Sae-Yeon
    • Journal of Yeungnam Medical Science
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    • v.14 no.2
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    • pp.350-358
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    • 1997
  • We studied the effects of adding a single bolus(500 mg) of sodium thiopental to a continuous infusion of low-dose fentanyl on plasma beta-endorphin immunoreactivity(iBE) responses to cardiopulmonary bypass(CPB) in 28 patients undergoing elective coronary artery bypass grafting or valve procedures. Thiopental was injected just prior to the initiation of CPB. The iBE levels and the hemodynamic indices such, as mean arterial pressure, cardiac output and systemic vascular resistance were measured before CPB, at 30 min and again at 60 min after the initiation of the bypass. The results were as follows. After the initiation of CPB, iBE levels increased at 30 min and 60 min(P=0.006, P=0.004 respectively) in the control group, but not in the thiopental group. There were significant differences in the changes of iBE levels between the groups(F=8.7, G-G=0.002, P=0.001). The hemodynamic indices were similar in both groups. In conclusion, pretreatment with thiopental just before the initiation of CPB prevents the stress-induced beta-endorphin response to CPB.

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Comparative Analysis of the Efficacy and Safety of Oxaliplatin Plus 5-Fluorouracil/Leucovorin (Modified FOLFOX6) with Advanced Gastric Cancer Patients having a Good or Poor Performance Status

  • Hacibekiroglu, Ilhan;Kodaz, Hilmi;Erdogan, Bulent;Turkmen, Esma;Esenkaya, Asim;Uzunoglu, Sernaz;Cicin, Irfan
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.6
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    • pp.2355-2359
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    • 2015
  • Background: Combination chemotherapy of 5 fluorouracil (5-FU) and leucovorin (LV) with oxaliplatin, mainly FOLFOX regimens, has shown considerable antitumor activity and a tolerable toxicity profile in gastric cancer. The goal of this study was to retrospectively compare the efficacy and toxicity of modified FOLFOX-6 (mFOLFOX6) regimen in advanced gastric cancer (AGC) patients with good and poor performance status (PS). Materials and Methods: AGC patients receiving the mFOLFOX6 regimen including oxaliplatin $85mg/m^2$, bolus of 5-FU $400mg/m^2$ and LV $400mg/m^2$ on the first day, followed by $2400mg/m^2$ of 5- FU as a continious infusion over 46 hour for first-line treatment were eligible for the study. Results: A total 58 patients with a median age of 59.5 (32-81) were included. The median follow up of the study was 9.2 months. Thirty patients (51.7%) with an ECOG PS 0-1 were assigned to the good PS arm, while 28 patients (48.3%) with ECOG PS 2 were in the poor PS arm. Overall response rates were 36.6 and 28.8%, respectively (p=0.91). Median PFS was 6.7 and 6.3 months in good PS and poor PS arms (p=0.50) and median OS was 9.6 and 10.4 months (p=0.55). As compared with good PS arm, poor PS arm was associated with more grade 3-4 neutropenia and anemia. Dose reduction and dose delays were also significantly higher. Conclusions: In this study, mFOLFOX6 was similarly effective in both arms. Although hematologic toxicity was significantly higher in patients with poor PS, it remained manageable. Our results suggest that this regimen may be an effective treatment option for AGC patients with poor PS.

Pharmacokinetic Modeling of Reversible Interconversion between Prednisolone and Prednisone (가역적상호대사과정 모델을 이용한 Prednisolone과 Prednisone의 약동학적 분석)

  • Shin, Jae-Gook;Yoon, Young-Ran;Cha, In-June;Jang, In-Jin;Lee, Kyung-Hoon;Shin, Sang-Goo
    • The Korean Journal of Pharmacology
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    • v.32 no.2
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    • pp.269-281
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    • 1996
  • Pharmacokinetics of prednisolone and prednisone undergoing reversible interconversion were analyzed from the model including this metabolic process. Blood samples were drawn serially upto 12 hours after I,V. bolus injection of 1 mg/kg prednisolone sodium phosphate and prednisone into 8 dogs as a crossover manner. Plasma concentrations of those two steroids were simultaneously measured with the method of HPLC. After injection, plasma concentrations of administered prednisolone and prednisone were declined with a biexponential pattern and their metabolic partner was rapidly formed. Plasma concentrations of those metaboite were decayed in parallel with their parent steroids throught the elimination phase. Apparent clearances of prednisolone and prednisone were $11.1{\pm}2.0\;ml/min/kg$ and $45.9{\pm}6.4\;ml/min/kg$, and they were underestimated by 29.4% and 33.6% compared to their real clearances$(15.7{\pm}4.4\;and\;69.2{\pm}17.7\;ml/min/kg)$ estimated using reversible interconversion model. Apparent volume of distribution of prednisolone$(1.32{\pm}0.43\;L/kg)$ and prednisone$(4.81{\pm}2.75\;L/kg)$ were overestimated by 53.5 and 52.7% and were compared to the real volumes $(0.86{\pm}0.30\;and\;3.15{\pm}2.13\;L/kg)$. Mean residence time of prednisolone$(2.0{\pm}0.61\;h)$ and prednisone$(1.74{\pm}0.74\;h)$ were much longer than the real sojourn time$(0.93{\pm}0.26\;and\;0.88{\pm}0.54\;h)$. Essential clearances In the reversible interconversion were greater as following orders: $Cl_{21}$(44.3 ml/min/kg) > $Cl_{20}$(24.2 ml/min/kg) > $Cl_{12}$ (7.9 ml/min/kg) > $Cl_{10}$(7.8 ml/min/kg). Estimated mean values of RF, EE, $%X^1_{ss}$ and $RHO^2_1$ were $0.31{\pm}0.10$, $1.49{\pm}0.23$, $69.3{\pm}16.7%$ and $0.65{\pm}0.10$, respectively. These results suggested that true pharmacokinetic parameters estimated from the model including reversible interconversion were significantly different from the apparent parameters estimated from the conventional mamillary model, and disposition of these two steroids seemed to be well explained by the model including reversible interconversion.

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Vasa Vasorum Densities in Human Carotid Atherosclerosis Is Associated with Plaque Development and Vulnerability

  • Joo, Sung-Pil;Lee, Seung-Won;Cho, Yong-Hwan;Kim, You-Sub;Seo, Bo-Ra;Kim, Hyung-Seok;Kim, Tae-Sun
    • Journal of Korean Neurosurgical Society
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    • v.63 no.2
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    • pp.178-187
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    • 2020
  • Objective : The extensive vasa vasorum network functions as a conduit for the entry of inflammatory cells or factors that promote the progression of angiogenesis and plaque formation. Therefore, we investigated the correlation between the carotid vasa vasorum activities and carotid plaque vulnerability using indocyanine green video angiography (ICG-VA) during carotid endarterectomy (CEA). Methods : Sixty-nine patients who underwent CEA were enrolled prospectively from September 2015 to December 2017. During CEA, a bolus of ICG was injected intravenously before and after resecting the atheroma. Additionally, we performed immunohistochemistry using CD68 (a surface marker of macrophages), CD117 (a surface marker of mast cells), and CD4 and CD8 (surface markers of T-cells) antibodies to analyze the resected plaque specimens. Results : The density of active vasa vasorum was observed in all patients using ICG-VA. The vasa vasorum externa (VVE) and interna (VVI) were seen in 11 (16%) and 57 patients (82.6%), respectively. Macroscopically, the VVE-type patterns were strongly associated with preoperative angiographic instability (81.8%, p=0.005) and carotid plaque vulnerability (90.9%, p=0.017). In contrast, the VVI-type patterns were weakly associated with angiographic instability (31.6%) and plaque vulnerability (49.1%). CD68-stained macrophages and CD117-stained mast cells were observed more frequently in unstable plaques than in stable plaques (p<0.0001, p=0.002, respectively). Conclusion : The early appearance of VVE, along with the presence of many microvessel channels that provided nutrients to the developing and expanding atheroma during ICG-VA, was strongly associated with unstable carotid plaques. The degree of infiltration of macrophages and mast cells is possibly related to the formation of unstable plaques.

SPECT Imaging of Dopamine Transporter with [I-123] IPT in Normal Controls and Parkinson's Patients (정상인과 파킨슨병 환자에서 [I-123]IPT SPECT를 이용한 도파민 재섭취부위의 영상화)

  • Sohn, Hyung Sun;Kim, Euy Neyng;Lee, Kyung Jin;Rha, Hyung Keun;Son, Byung Chul;Choi, Chang Rhack
    • Journal of Korean Neurosurgical Society
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    • v.30 no.3
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    • pp.342-348
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    • 2001
  • Objective : Dopamine transporter concentrations have been known to decrease in Parkinson's disease(PD). The aim of the present study was to evaluate the correlation between SPECT measurements of [I-123]N-(3-iodopropene-2-yl)-$2{\beta}$-carbomethoxy-$3{\beta}$-(4-chlorophenyl) tropane(IPT) as an imaging agent for measuring changes in transporter concentrations with PD. Patients and Methods : IPT labelled with $4.87{\pm}1.29mCi$($180.19{\pm}47.73MBq$) of [I-123] was intravenously injected into 23 patients(age : $58{\pm}12$) with PD and three normal controls(NC)(age : $37{\pm}7$) as bolus. Brain SPECT were then performed at 1 hour and 2 hours after injection on a double headed camera. The statistical parameters were the contrast ratio of left basal ganglia(BG) and right basal ganglia to occipital cortex(OCC) per milli curies of injected radiotracer at 1 hour and 2 hours. The correlations were evaluated between these parameters and Hoehn-Yahr classification of the patients. Results : The(BG - OCC)/OCC/mCi ratios at 1 hour and 2 hours for PD and NC were $0.14{\pm}0.07$ and $0.27{\pm}0.07$(1 hour) and $0.12{\pm}0.07$ and $0.34{\pm}0.04$(2 hour), respectively. The(BG - OCC)/OCC/mCi ratios of Parkinson's disease were decreased with higher grade of Hoehn-Yahr classification of the patients. The ratio between BG and OCC for PD were clearly separated from NC and may be useful outcome measures for clinical diagnosis. Conclusion : The findings suggest that IPT may be a very useful tracer for early diagnosis and treatment of PD and study of dopamine re-uptake site.

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