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Association of Insulin-related Genes Expression with Carcass Weight in Loin Muscle of Korean Cattle (Hanwoo) (한우 등심조직 내 인슐린 조절 유전자의 발현이 도체중에 미치는 영향에 관한 연구)

  • Lim, Dajeong;Cho, Yong-Min;Chai, Han-Ha;Lee, Seung-Hwan;Choi, Bong-Hwan;Kim, Nam-Kuk
    • Journal of Life Science
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    • v.25 no.1
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    • pp.8-15
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    • 2015
  • The peroxisome proliferator-activated receptor (PPAR) signaling pathway is well known as a candidate pathway related to meat quality in mammals. In particular, there are many studies on the relationship between the PPAR signaling pathway and intramuscular fat. However, recent studies have demonstrated that genes in the PPAR signaling pathway are associated with carcass weight in cattle. Among 48 genes in the PPAR signaling pathway, 16 genes are related to the insulin that regulates the adipocyte glucose metabolism and thus affects body weight. Therefore, we conducted an investigation to try to identify candidate genes associated with the carcass weight and relationships between the expressions of these 16 genes in the loin muscle of Hanwoo (Korean cattle). From regression analysis, the three genes (ACSL6, FADS2, and ILK) showed significant effects with regard to carcass weight (p<0.05). Finally, we analyzed the common regulators of the significant genes from pathway analysis. The significant genes are regulated by insulin as well as D-glucose. These findings show that the differentially expressed genes are possible candidate genes associated with carcass weight in the longissimus muscle of Korean cattle.

Studies on Feed Value of Waste Rice Straws for Broiler Chicken (육계병아리 사육에 있어서 느타리버섯 재배 폐볏짚의 사료가치에 관한 연구)

  • 오세정;김법회;이택원;조성구
    • Korean Journal of Poultry Science
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    • v.11 no.2
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    • pp.115-125
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    • 1984
  • The Waste Rice Straws (W.R.S.) after cultivation with the Pleurotas Ostreatus SACCARDO was substituted for wheat bran in the broiler ration. The results of the experiment 1 and 2 obtained were summerized as follows; 1. The content of C. protein in the W.R.S. showed 1.5% higher value than that in the rice straw. No change was detected in the content of C. fat. However the content of C. fiber and N.F.E in W.R.S. decreased in comparision with those of raw rice straw. 2. The treated groups were inclned to rapid gain of body weight, especially the 4% W.R.S. group had been showed significatly higher value of gain than the control and 6% treated groups (p<0.05 ). 3. No significant differences were observed in the amount of feet intake among experimental groups, even if the treated groups showed slightly higher intake than control group. 4. The treated groups in W.R.S. were inferior to the control group in feed intake but there were no significant differences. 5. The methionine in W.R.S. was the only amino acid which contained similar quantity of wheat brans. 6. To extend the amount of W.R.S. in broiler ration the 6% of W.R.S. was substituted for wheat bran and methionine mixture was added. No significance differences had been showed in weight gain, feed intake and feed efficiency between control and treated groups. Even though the treated groups showed more feed intake and lower weight gain in comparision with control group. 7. The methionine added group showed adverse effect in economics of production (p<0.01) because of high cost of the methionine mixture, Therefore less than. 6% of the WRS could be substituted for wheat bran. According to the results of the experiment 1 and 2 it could be concluded that maximum use of the W.R.R. as substitute for wheat bran was less than 4% of the ration.

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Nutrient Digestibilities and Fecal Characteristics of Diets Including Brown Rice for Miniature Schnauzer (Miniature Schnauzer에 있어서 현미 급여와 영양소 소화율 및 배설 분 특성)

  • Kim, Kyoung-Hoon;Chang, Ju-Song;Oh, Young-Kyoon
    • Journal of Animal Science and Technology
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    • v.53 no.5
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    • pp.429-434
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    • 2011
  • The objectives of this research were to determine the effects of brown rice (BR) on food intake, digestion, energy value, and fecal characteristics. Three replacement levels which BR replaced 0, 15 and 30% of wheat flour were tested. Six female Miniature Schnauzer (8~9 month age, initial mean body weight 5.0 ${\pm}$ 0.3 kg) were assigned to treatments in replicated $3{\times}3$ Latin square design. Total tract digestibilities of DM, OM, acid hydrolyzed fat and gross energy except CP increased linearly (P<0.01), and observed digestible energy and metabolizable energy values also increased linearly (P=0.001 and P=0.006, respectively) with increasing BR replacement level. Wet and dry fecal output decreased linearly (P<0.001, P=0.004) with increasing BR inclusion to the diets and BR 30% treatment reduced wet fecal output up to 21% of that of control. Quadratic (P<0.01) effects was observed in fecal score for dog fed BR and fecal ammonia concentration tended to increase linearly (P=0.07) in response to increasing BR replacement level. It seems that the increase in fecal ammonia concentration may be partially related to the decrease in shortchain fatty acid concentration (P=0.001). This study clearly demonstrates that BR improves nutrients digestibility and fecal characteristics of dog.

Nutrient Digestibility, Palatability and Stool Quality of Canine Food Including Brown Rice (현미 함유 반려견 사료의 영양소 소화율, 분 특성 및 기호성)

  • Kim, Kyoung-Hoon;Chang, Ju-Song;Oh, Young-Kyoon;Ji, Sang-Yun;Moon, Sang-Ho;Kim, Myeong-Hwa
    • Journal of Animal Science and Technology
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    • v.53 no.5
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    • pp.435-440
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    • 2011
  • In this experiment, two inclusion levels (15 and 30% of diets, as-fed basis) of brown rice (BR) were tested against a control diet with 0% BR (51% wheat flour of diet). Six female Maltese (8~9 months age, initial mean body weight of $2.8{\pm}0.3$ kg) were assigned to treatments in replicated $3{\times}3$ Latin square design. Total tract digestibilities of DM, OM, acid hydrolyzed fat, CP and gross energy by dogs fed experimental diets responded quadratically (P<0.01 or <0.05) to BR inclusion levels. Similarly, quadratic responses (P<0.001, P=0.015) were observed for digestible energy and metabolizable energy values, respectively. Wet and dry fecal output also exhibited quadratic responses (P=0.006, P=0.014, respectively) but no differences were observed between control and BR 30% diets. Linear (P=0.008) effect was observed for fecal score in dogs fed BR, and fecal ammonia concentration increased linearly (P=0.001) in response to increasing BR inclusion levels. It seems that the increase in fecal ammonia concentration may be partially related to the decrease tendency (P=0.07) in short-chain fatty acid concentration. In this experiment, inclusion of BR in dog diet did not result in pronounced changes in the digestibility but in fecal score. Although inclusion of BR in diets showed profound positive effect on fecal score, it is confounded by the high inclusion level of poultry offal meal.

Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats

  • Adeyemi, Kazeem D.;Shittu, Rafiat M.;Sabow, Azad B.;Abubakar, Ahmed A.;Karim, Roselina;Karsani, Saiful A.;Sazili, Awis Q.
    • Journal of Animal Science and Technology
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    • v.58 no.6
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    • pp.23.1-23.17
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    • 2016
  • Background: The functionality of myofibrillar proteins is a major factor influencing the quality attributes of muscle foods. Nonetheless, the relationships between muscle type and oxidative changes in chevon during ageing are meagrely elucidated. Postmortem changes in antioxidant status and physicochemical properties of glycolytic gluteus medius (GM) and oxidative infraspinatus (IS) muscles in goats were compared. Methods: Twenty Boer bucks (9-10 months old, body weight of $36.9{\pm}0.725kg$) were slaughtered and the carcasses were subjected to chill storage ($4{\pm}0.5^{\circ}C$). Analyses were conducted on GM and IS muscles sampled on 0, 1, 4 and 7 d postmortem. Results: Chill storage did not affect the antioxidant enzyme activities in both muscles. The IS had greater (P < 0.05) superoxide dismutase and catalase activities than GM. Carotenoid and tocopherol contents did not differ between muscles but decreased (P < 0.05) over storage. The IS had higher (P < 0.05) glycogen and ultimate pH and lower (P < 0.05) shear force and cooking loss than GM. The carbonyl content, % metmyoglobin, drip loss and TBARS increased (P <0.05) while free thiol, metmyoglobin reducing activity (MRA), shear force and myoglobin decreased (P < 0.05) over storage. Muscle type had no effect (P > 0.05) on free thiol, MRA and TBARS. The GM had lower (P < 0.05) redness on d 0 and 1 than IS while the IS had greater carbonyl, % metmyoglobin and drip loss than GM on d 7. The reflective density of slow myosin heavy chain (MHC) was higher (P < 0.05) while the density of fast MHC and actin was lower (P < 0.05) in IS than GM. Regardless of muscle type, the density of MHC decreased (P < 0.05) while that of actin was stable over storage. Nonetheless, the degradation of fast and slow MHC was greater (P < 0.05) in IS than GM. Muscle type had no effect (P > 0.05) on consumer preference for flavour, juiciness and overall acceptability. However, IS had higher (P < 0.05) tenderness score than GM on d 1 and 4 postmortem. Intramuscular fat was higher (P< 0.05) in IS compared with GM. Fatty acid composition did not differ between the muscles. However, GM had lower (P < 0.05) n-6/n-3 ratio than IS. The n-3 and n-6 PUFA declined (P < 0.05) while the SFA increased (P < 0.05) over storage. Conclusion: The changes in myofibrillar proteins and physicochemical properties of goat meat during postmortem chill storage are muscle-dependent.

Effects of dietary energy and lysine levels on physiological responses, reproductive performance, blood profiles, and milk composition in primiparous sows

  • Hong, Jinsu;Fang, Lin Hu;Kim, Yoo Yong
    • Journal of Animal Science and Technology
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    • v.62 no.3
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    • pp.334-347
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    • 2020
  • The adequate intake of energy and lysine for primiparous sows are necessary for maternal growth of sows and growth of their progeny. This study was conducted to evaluate the effects of dietary energy and lysine levels on primiparous sows and their progeny. A total of 48 gilts (Yorkshire × Landrace), with an initial body weight (BW) of 168.1 ± 9.71 kg and at day 35 of gestation, were allotted to eight treatment groups with a 2 × 4 factorial arrangement. The first factor was metabolizable energy levels in diet (3,265 or 3,365 kcal of ME/kg), and the second factor was lysine levels in diet (gestation 0.55%, 0.65%, 0.75%, 0.85%, lactation 0.70%, 0.85%, 1.00%, 1.15%). The BW gain (p = 0.07) and backfat thickness (p = 0.09) in the gestation period showed a tendency to be increased in sows fed the high-energy diets. In the lactation period, sows fed the high-energy diets tended to be greater BW (p = 0.09) and less BW loss (p = 0.05) than those of sows fed the low-energy diets. Sows fed high-energy diets had a tendency of greater piglet weight at day 21 of lactation and greater piglet weight gain (p = 0.08 and p = 0.08, respectively). Although the blood urea nitrogen (BUN) was increased linearly as dietary lysine level increased at day 110 of gestation (Linear, p = 0.03), the BUN was decreased linearly as dietary lysine level increase at day 21 of lactation (Linear, p < 0.01). In the composition of colostrum, sows fed high-energy diets had greater casein, protein, total solid, solid not fat, and free fatty acid concentrations than those of sows fed low-energy diets (p < 0.05). Supplementation of total lysine 0.75% for gestation and 1.00% for lactation with 3,365 kcal of ME/kg energy level could be applied to the primiparous sows' diet to improve performance of sows and growth of their progeny.

Sarcopenia and Sarcopenic Obesity and Their Association with Cardiovascular Disease Risk in Postmenopausal Women : Results for the 2008-2011 Korea National Health and Nutrition Examination Survey (폐경 여성의 근감소증 및 근감소성비만과 심혈관질환 위험도와의 관련성 연구: 국민건강영양조사(2008-2011) 자료를 활용하여)

  • Kim, Misung;Sohn, Cheongmin
    • Korean Journal of Community Nutrition
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    • v.21 no.4
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    • pp.378-385
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    • 2016
  • Objectives: This study was conducted to investigate the association between sarcopenia and sarcopenic obesity and cardiovascular disease risk in Korean postmenopausal women. Methods: We analyzed data of 2,019 postmenopausal women aged 50-64 years who participated in the Korea National Health and Nutrition Examination Survey in 2008-2011 and were free of cardiovascular disease history. Blood pressure, height, and weight were measured. We analyzed the serum concentrations of glucose, total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and triglyceride levels. Waist circumference was used to measure obesity. Appendicular skeletal muscle mass was measured by dual-energy X-ray absorptiometry. Sarcopenia was defined as the appendicular skeletal muscle mass/body weight<1 standard deviation below the gender-specific means for healthy young adults. The estimated 10-year risk of cardiovascular disease risk was calculated by Pooled Cohort Equation. Subjects were classified as non-sarcopenia, sarcopenia, or sarcopenic obesity based on status of waist circumference and appendicular skeletal muscle mass. Results: The prevalence of sarcopenia and sarcopenic obesity was 16.3% (n=317) and 18.3% (n=369), respectively. The 10-year risk of cardiovascular disease risk in the sarcopenic obesity group was higher ($3.82{\pm}0.22%$) than the normal group ($2.73{\pm}0.09%$) and sarcopenia group ($3.17{\pm}0.22%$) (p < 0.000). The odd ratios (ORs) for the ${\geq}7.5%$ 10-year risk of cardiovascular disease risk were significantly higher in the sarcopenic obesity group (OR 3.609, 95% CI: 2.030-6.417) compared to the sarcopenia group (OR 2.799, 95% CI: 1.463-5.352) (p for trend < 0.000) after adjusting for independent variables (i.e., exercise, period of menopausal, alcohol use disorders identification test (AUDIT) score, income, education level, calorie intake, %fat intake and hormonal replacement therapy). Conclusions: Sarcopenia and sarcopenic obesity appear to be associated with higher risk factors predicting the 10-year risks of cardiovascular disease risk in postmenopausal women. These findings imply that maintaining normal weight and muscle mass may be important for cardiovascular disease risk prevention in postmenopausal women.

Effects of Worksite Nutrition Counseling for Health Promotion; Twelve-Weeks of Nutrition Counseling Has Positive Effect on Metabolic Syndrome Risk Factors in Male Workers (직장인 남성을 대상으로 한 12주간의 영양교육이 대사증후군 관련 인자에 미치는 영향)

  • Lee, Mi-Sun;Kang, Hae-Jin;Oh, Hye-Sun;Paek, Yun-Mi;Choue, Ryo-Won;Park, Yoo-Kyoung;Choi, Tae-In
    • Korean Journal of Community Nutrition
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    • v.13 no.1
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    • pp.46-61
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    • 2008
  • The purpose of this study was to investigate the effect of nutrition counseling on improving metabolic syndrome (MS) risk factors. Sixty-eight subjects were grouped according to their numbers of MS risk factors. Subjects who have three or more risk factors of MS were defined as "High risk", subjects who have two risk factors of MS were defined as "low risk", and subjects who have below two risk factors of MS were defined as "no risk" group. All groups finished nutrition counseling every three weeks for 12 weeks. Anthropometric, dietary assessments (24 hr-recall) and blood samples were measured at 0 and 12 weeks nutrition counseling. After 12 weeks of intervention, anthropometric data (weight, BMl, body fat (%), and waist/hip ratio) were significantly decreased (p < 0.05) in all groups. Daily consumption of calorie was decreased significantly (p < 0.05) in the group of low risk and high risk. Blood level of fasting plasma glucose was significantly decreased (p < 0.001) in all groups after 12 weeks of intervention. Significantly, the fasting plasma glucose level was returned to normal range in the high risk group. The number of people who have three or more risk factors of MS (high risk group) was decreased from 25 to 12. Sum of MS Criteria decreased from 85 to 52 in the group of MS and decreased from 143 to 99 in all groups. These results indicate that nutrition counseling for male workers at the worksite proved to be helpful by reducing the risk factors of MS and thereby reducing the risk of cardiovascular disease. Continuing and systematic nutritional management programs should be developed and implemented for male workers at the worksites.

Hypoglycemic Effect of Mushroom Fermented Milk in Streptozotocin-Induced Diabetic Rats (당뇨성 흰쥐에서 버섯 추출물 함유 발효유 첨가 식이의 혈당강하작용)

  • Cha, Jae-Young;Jeon, Beong-Sam;Park, Jeong-Won;Shin, Gab-Gyun;Kim, Beom-Kyu;Kim, Hee-Kyu;Cho, Young-Su
    • Journal of Life Science
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    • v.14 no.4
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    • pp.676-682
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    • 2004
  • Nutritional concentrations by chemical analyses of mushroom fermented milk were protein 2.87%, fat 0.09%, carbohydrates 6.0%, dietary fiber 0.3%, lactose 2.01%, sucrose 1.23%, calcium 95.9 mg/100 g and iron 0.08 mg/100 g. The present study was undertaken to investigate the hypoglycemic effects of the equal volume of either water (streptozotocin (STZ)rontrol rats), mushrooms water-extract (STZ-extrart fed rats), mushroom fermented milk product (STZ-mushroom yogurt fed rats) or mushroom fermented milk supernatant (STZ-supernatant fed rats) (10%, v/w), in STZ-induced diabetic rats for 3 week period. The mushroom fermented milk given to the STZ-diabetic rats decreased the blood glucose significantly and increased the blood insulin, compared with the STZ-control rats. The supernatant and mushroom water extract also slightly retarded the development of hyperglycemia in the STZ-diabetic rats. Taken together the results, the mushroom yogurt may have a potential for the hypoglycemic effect in the STZ-diabetic rats.

Effects of Feeding Browses on Growth and Meat Quality of Korean Native Goats (지엽류 급여가 흑염소의 발육 및 육질에 미치는 영향)

  • Choi, S.H.;Park, B.Y.;Cho, Y.M.;Choi, C.Y.;Kwon, E.G.;Kim, Y.K.;Her, S.N.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.819-824
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    • 2003
  • Effects of feeding browses on growth and meat quality of Korean native goats were investigated. Twenty eight heads of male goats were divided into four treatment groups fed diets containing oak browse, pine browse, fermented pine browse, and rice straw. Growth rate, feed intake, carcass and meat quality and blood and serum criteria were investigated. The highest average daily gain of goats was 45.3g from oak browse, and goats on pine browse gained similar to those on fermented pine browse. Daily forage intake was 197g DM for oak browse and 74g DM for fermented pine browse. Feed efficiency(g feed/g gain) of oak browse was 11.0g and that of pine browse was 15.1g. From the results of serological inspection, metabolism in body was normal with all the browses tested. Dressing percentage was 45.1~46.2% and meat percentage was 55.2~55.9% without significant differences between diets. Fat percentage of goat carcasses fed rice straw was higher than those fed pine browse or fermented pine browse which was, however, not significantly different from those on oak browse. Shear force and coking loss of goat meat on pine browse or fermented pine browse was less than those on rice straw(p〈0.05) without significant differences between other browses. Juiciness, tenderness, and flavor of the goat meat were the better(p<0.05) from the pine browse diet than from rice straw.