• Safety and Health at Work
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• 제15권1호
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• pp.118-122
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• 2024

To understand biosafety's current situation in laboratory animal research and risk factors affecting occupational health. Compliance surveys were conducted by questionnaire via Questionnaire Star (an application app on the Internet) in Chinese. Thirty-nine anonymous questionnaires were collected. The surveyed institution has established 24 types of ABSL (Animal Biosafety Laboratory) and biosafety management organizations and systems equipped with safety equipment. Our study also suggests that the principal of the laboratory establishment fails to perform supervision and inspection responsibilities, the inappropriate design of the animal biosafety laboratory, non-standardized personnel training and health management, non-strict waste management, and insufficient emergency management. The administrative department and work units should address certain safety and occupational health risks in laboratory animal research. The author proposes control strategies based on organizational guarantee, personnel management, emergency management, etc., to help prevent risks and ensure occupational health. Due to regional limitations and small sample size, the results may not be generalisable to all parts of the world. However, some of the key common issuesmay also be present in other regions, sowe believe that this research still has some relevance.

• Journal of Plant Biotechnology
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• 제35권2호
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• pp.101-108
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• 2008

아그로박테리움을 이용한 형질전환은 대다수의 쌍자엽과 몇몇 단자엽 식물의 게놈내로 외래유전자를 도입하는 성공적인 방법이다. 해충저항성 CryIAc 유전자가 도입된 배추형 질전환체를 아그로박테리움 형질전환법을 통해 얻은 후, 도입유전자의 수를 Southern 분석을 통하여 확인하였다. 배추형질전환체 46개 중에서 29개는 1 copy의 CryIAc 유전자가 도입된 것으로 확인되었다. 식물체의 게놈내로 T-DNA 결합에 관한 정보를 얻기 위해서 LB 인접서열을 genome walking PCR 방법을 통하여 분석하였다. 46개의 배추형질전환체중에서 37개는 운반체의 backbone 염기서열을 지니는 것으로 확인되었다. 이러한 결과는 도입 운반체의 LB 지점에서 제대로 종결이 이루어지지 않아서 운반체의 backbone 염기서열이 운반된 것으로 보여진다. 운반체의 backbone 염기서열이 도입되지 않은 9개체의 배추형질전환체를 LB 인접서열을 분석한 결과, 모든 LB 부위는 절단부위가 보존되지 않았고, 절단부위에서 36bp까지도 결실이 확인되었다.

• Journal of Plant Biotechnology
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• 제5권1호
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• pp.13-17
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• 2003

Biotechnology is certainly one of the major landmarks in the 20th century history of science. It may produce enormous utility to human beings, but at the same time, it carries huge potential risks to the environment and public health. Thus, with a view to securing safety for the environment and public health in relation to the development and use of living modified organisms (LMOs), the Cartagena Protocol on Biosafety was adopted, in which a regulation Procedure as to the transboundary movements, transportation, handling, and use of LMOs were drawn up. In order to prepare for the entry into force of the Protocol, the Republic of Korea legislated the "Act on the Transboundary Movements of living modified organisms(hereinafter referred as the 'Act')" in Mar 2001, and has pre-announced the enactments of the enforcement ordinance and the enforcement regulation to the Act. Pursuant to the Act, the Ministry of Commerce, Industry and Energy, as a Competent National Authority, is making efforts to implement domestic biosafety schemes in cooperation with other bio-related government ministries. In order for these efforts to reap fruits, industry, academia, and research institutions should cooperate with one another, and civic groups and NGOs should narrow the differences in opinions and timely respond to the fast-changing situations. Focusing on the precautionary principle, the Protocol puts a great emphasis on the importance of information sharing amongst countries, and the Act also follows this principle. In order to gurantee biosafety, countries around the world, including the ROK, agreed to establish National Biosafety Clearing, designed to provide the information on the export/import of LMOs, R'||'&'||'Ds, risk assessment, safety control, etc. and register it on the CBD Central Biosafety Clearing House.e.

• 대한수의학회지
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• 제33권2호
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• pp.289-293
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• 1993

Following the death of more than 250 goats in one herd of 800 goats, imported from Australia, an epidemiological investigation was undertaken to determine the probable aetiology of this apprently mysterious disease. The syndrome was characterized by severe anemia(Hematocrit <20% ; normal range 24 to 48). All the affected animals were imported from Australia and all the motalities occurred during the period from September to November, 1992 Giemsa stain, acridine orange and indirect immunoflourescence tests were utilized in a survey involving 239 goats reared in Chonbuk Province. The positivity rates by acridine orange for anaplasmosis or piroplasmosis were 60.8% and 66.2% for imported and indigenous breeds respectively. It is tenatively concluded that the probable cause of death was anaplasmosis.

• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.439-446
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• 2022

콩은 양질의 단백질과 지방산이 풍부하며, 세계적으로 가장 많이 사용되는 형질전환작물(GM) 중 하나이다. 국내에서 GM콩은 주로 광안을 모본으로 하여 개발되고 있는 상황이다. 본 연구에서는 비표적 LC-MS 기반 대사체 분석기술을 이용하여 2020년도에 군위와 전주에서 재배한 광안과 세 일반콩의 대사체 프로파일을 비교분석 하였다. Partial least square-discriminant analysis (PLS-DA) 분석을 통하여 대사체 프로파일들은 품종별로 잘 분리되었으며, 페닐알라닌과 이소플라본, 지방산을 포함하여 18종 물질이 관여하는 것으로 확인하였다. PLS-DA 스코어 플롯에서 콩 4품종은 지역별로 클러스터를 형성하였으며, 이는 재배환경이 대사물질의 변화에 영향을 준 것으로 판단된다. 광안은 다른 품종들에 비하여 이소플라본 함량이 가장 낮았으며, 리놀렌산 함량은 가장 높았다. 광안을 이용하여 개발된 생명공학콩의 실질적동등성 평가의 경우 광안의 대사체 프로파일 특성을 고려한 비교품종 선정 등에 관하여 고찰하였다.

• Journal of Ginseng Research
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• 제44권2호
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• pp.308-311
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• 2020

Extracts of ginseng species show antihyperglycemic activity. We evaluated the inhibitory effects of diabetic complications for Korean Red Ginseng (KRG), which is enriched in ginsenosides using Otsuka Long-Evans Tokushima Fatty (OLETF) rats. The animals were divided into one of four groups (n = 6~9): Long-Evans-Tokushima-Otsuka rats (control rats), OLETF rats, rats given 200 mg/kg KRG, and rats given 400 mg/kg KRG. We examined the protective potential of KRG against type 2 diabetic illnesses. The results exhibited that KRG showed significant antihyperglycemic and antioxidative effects in KRG-treated OLETF rats. And, our results proposed the amelioration of cardiac function through normalized ejection fraction, fractional shortening, and vascular reactivity. Furthermore, histopathological abnormalities in the OLETF rats were prevented by KRG treatment.

• 대한임상검사과학회지
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• 제53권1호
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• pp.68-80
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• 2021

An uncertified facility is a facility not certified, as defined in the Biological Agents and Toxins Act (BATA) in Singapore, but has met the requirements of the Ministry of Health to possess First and Fifth Schedule biological agents and toxins. This type of facility is also known as a Biosafety Level 2 Plus (BSL 2+) facility. Registration as an uncertified facility or a BSL 2+ facility requires a certain process and procedure to be sought with the Biosafety Branch of the ministry. This review, shares first-hand knowledge on the journey to achieving registration of the authors' facility. The procedure involved considerable preparation, setting up facility requirements, biosafety precautions, procedures and practices, and training and competence of laboratory users. The ministry conducted a thorough onsite facility audit to ensure that the facility requirements and biosafety procedures and practices were in place. It then issued an approval letter of possession for the first-time use of biological agents and registered the laboratory as an uncertified facility. The expectation is that the comprehensive information shared may be of great benefit to other facilities with similar interests.

• 한국어병학회지
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• 제33권2호
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• pp.139-143
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• 2020

This study investigated the disinfection effect of monopersulfate (MPS) compound against white spot syndrome virus (WSSV) by bioassay using kuruma shrimp (Marsupenaeus japonicas). A WSSV stock was prepared with muscle homogenate from WSSV-infected whiteleg shrimp (Penaeus vannamei) and its lethal dose 50% endpoint (LD50) and infectious dose 50% endpoint (SID50) were respectively determined as 10-5.63 and 10-6.79 in bioassay using kuruma shrimp, followed by PCR assays. The disinfective effect of MPS compound (1.2 ppm, 2.4 ppm, 4.8 ppm) was performed by bioassay using about 10-fold higher dilution (10-4) of WSSV homogenate. The compound resulted in WSSV inactivation by a concentration-dependent manner. In addition, 4.8 ppm of MPS completely prohibited WSSV infection. To our knowledge, this study is the first report about the usefulness of MPS as a disinfectant to WSSV.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.85.1-86
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• 2005

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2008년도 하계학술발표대회 논문집
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• pp.84-89
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• 2008

The biosafety laboratory HVAC control technology may be applied in order to protect contamination of the researcher, supervisor and to prevent diffusion of biological pollution. In this study, a biosafety level, general configuration of control system, differential pressure control, distributed control system and network structure were discussed. These systems able to increase laboratory safety and efficiency of HVAC system.