• Title/Summary/Keyword: biomaterials

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Analysis of Immunomodulating Activities in Methanol Extracts from Several Kinds of Grapes (포도 품종별 메탄올 추출물로부터 면역활성 분석)

  • Heo, Jin-Chul;Woo, Sang-Uk;Kweon, Mi-Ae;Kim, Bo-Bae;Lee, Sook-Hee;Lee, Jin-Man;Choi, Jong-Uck;Chung, Shin-Kyo;Lee, Sang-Han
    • Food Science and Preservation
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    • v.14 no.4
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    • pp.419-424
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    • 2007
  • Fruits and vegetable extracts are well-known as healthy foods. Such foods have been used as herbal medicines or traditional therapies for centuries. To assess biological activities in grapes, we examined the immunomodulating activities of water extracts from four kinds of grapes (cultivars Kyoho, Delaware, Campbell, and Niagara). We explored possible antioxidant and anticancer activities using antioxidant assays such as the 1,1-diphenyl-2-picrylhydrazyl (DPPH) reduction assay, the ferric iron reducing ability of plasma (FRAP) assay, a cell proliferation assay, an NO inhibition assay, a wound healing assay, and an IL-4/IL-13 elicitation assay. Methanol extracts of grapes were tested. The results showed that each grape extract had potent antioxidant activities. The grape extracts increased cell proliferation and NO production activities in tumor cell lines. IL-4 and IL-13 cytokine levels were decreased in mouse primary spleen cells by treatment with any extract. These results suggest that grape extracts can be used as biomaterials with immunomodulating activities.

In vitro Antibacterial Effect of Orthodontic Adhesives Mixed with Silver Nanoparticles

  • Choi, Kwon-Yong;Lee, Jong-Chan;Hwang, Yun-Chan;Chang, Seok-Woo;Kum, Kee-Yeon;Bae, Kwang-Shik;Lim, Bum-Soon;Paeng, Jun-Young;Kim, Eun-Cheol;Kwon, Ho-Beom;Kim, Young-Ho
    • Journal of Korean Dental Science
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    • v.5 no.1
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    • pp.7-12
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    • 2012
  • Purpose: To examine the antibacterial effectiveness of silver nanoparticles (SNP) mixed with commercial orthodontic adhesives. Materials and Methods: SNP was prepared by dissolving silver perchlorate in an organic solvent and reducing it with ultraviolet radiation. SNP was then mixed with four commercial orthodontic adhesives (Light Bond, Blugloo, Transbond XT, and Fuji Ortho LC) (0.05 wt %), which were then formed into disc-shape specimens ($8.0mm{\times}3.0mm$). Commercial orthodontic adhesives containing no SNP were used as the control groups. Specimens of the four experimental and four control groups were incubated with streptococcus mutans and the medium turbidity was assessed at 3, 6, 9, 12, and 24 hours after incubation. The agar diffusion test was also performed to examine the growth inhibition zone of these groups. The data were statistically analyzed using a Wilcoxon rank sum test and t-test with a Bonferroni's correction (P<0.05). Result: The SNP containing groups had a superior antibacterial effect compared to the control groups. In the agar diffusion test, the control groups without SNP did not produce an inhibition zone, whereas the SNP containing groups showed inhibition zone of 10~13 mm. Conclusion: The incorporation of SNP into orthodontic adhesives can inhibit cariogenic bacterial growth.

A study on the safety and efficacy of bovine bone-derived bone graft material(OCS-B) (생체 유래 골 이식재(OCS-B)의 안전성 및 유효성에 관한 연구)

  • Park, Ho-Nam;Han, Sang-Hyuk;Kim, Kyoung-Hwa;Lee, Sang-Chul;Park, Yoon-Jeong;Lee, Sang-Hoon;Kim, Tae-Il;Seol, Yang-Jo;Ku, Young;Rhyu, In-Chul;Han, Soo-Boo;Chung, Chong-Pyoung
    • Journal of Periodontal and Implant Science
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    • v.35 no.2
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    • pp.335-343
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    • 2005
  • Inorganic bovine bone mineral has been widely researched as bone substitution materials in orthopedic and oral and maxillofacial application. OCS-B(NIBEC, Korea) is newly-developed inorganic bovine bone mineral. The aim of this study is to evaluate the safety and efficacy of bovine bone-derived bone graft material(OCS-B). Micro-structure of newly-developed inorganic bovine bone mineral(OCS-B) was analyzed by scanning electron microscope(SEM). Round cranial defects with eight mm diameter were made and filled with OCS-B in rabbits. OCS-B was inserted into femoral quadrant muscle in mouse. In scanning electron microscope, OCS-B was equal to natural hydroxyapatite. Rabbits were sacrificed at 2 weeks and 4 weeks after surgery and mice were sacrificed at 1 week and 2 weeks after surgery. Decalcified specimens were prepared and observed by microscope. In calvarial defects, osteoid and new bone were formed in the neighborhood of OCS-B at 2 weeks after surgery. And at 4 weeks after surgery osteoid and new bone bridge formed flourishingly. No inflammatory cells were seen on the surface of OCS-B at 1 week and 2 weeks in mouse experimental group. It is concluded that newly-developed inorganic bovine bone mineral(OCS-B) is a flourishing bone-forming material and biocompatible material.

Effect of metal conditioner on bonding of porcelain to cobalt-chromium alloy

  • Minesaki, Yoshito;Murahara, Sadaaki;Kajihara, Yutaro;Takenouchi, Yoshihisa;Tanaka, Takuo;Suzuki, Shiro;Minami, Hiroyuki
    • The Journal of Advanced Prosthodontics
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    • v.8 no.1
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    • pp.1-8
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    • 2016
  • PURPOSE. The purpose of this study was to evaluate the efficacy of two different metal conditioners for non-precious metal alloys for the bonding of porcelain to a cobalt-chromium (Co-Cr) alloy. MATERIALS AND METHODS. Disk-shaped specimens ($2.5{\times}10.0mm$) were cast with Co-Cr alloy and used as adherend materials. The bonding surfaces were polished with a 600-grid silicon carbide paper and airborne-particle abraded using $110{\mu}m$ alumina particles. Bonding specimens were fabricated by applying and firing either of the metal conditioners on the airborne-particle abraded surface, followed by firing porcelain into 5 mm in diameter and 3 mm in height. Specimens without metal conditioner were also fabricated. Shear bond strength for each group (n=8) were measured and compared (${\alpha}=.05$). Sectional view of bonding interface was observed by SEM. EDS analysis was performed to determine the chemical elements of metal conditioners and to determine the failure modes after shear test. RESULTS. There were significant differences among three groups, and two metal conditioner-applied groups showed significantly higher values compared to the non-metal conditioner group. The SEM observation of the sectional view at bonding interface revealed loose contact at porcelain-alloy surface for non-metal conditioner group, however, close contact at both alloy-metal conditioner and metal conditioner-porcelain interfaces for both metal conditioner-applied groups. All the specimens showed mixed failures. EDS analysis showed that one metal conditioner was Si-based material, and another was Ti-based material. Si-based metal conditioner showed higher bond strengths compared to the Ti-based metal conditioner, but exhibited more porous failure surface failure. CONCLUSION. Based on the results of this study, it can be stated that the application of metal conditioner is recommended for the bonding of porcelain to cobalt-chromium alloys.

CD34 Monoclonal Antibody-Immobilization on Polyurethane Surface by Poly(PEGA-co-BMA) Coating (PEGA/BMA 공중합체의 코팅을 통해 CD34 단일클론항체가 고정화된 폴리우레탄 표면)

  • Joung, Yoon-Ki;Hwang, In-Kyu;Park, Ki-Dong
    • Polymer(Korea)
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    • v.33 no.6
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    • pp.602-607
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    • 2009
  • A polyurethane (PU) surface enabling in vivo endothelialization via endothelial progenitor cell (EPC) capture was prepared for cardiovascular applications. To introduce CD34 monoclonal antibody (mAb) inducing EPC adhesion onto a surface, poly (poly (ethylene glycol) acrylate-co-butyl methacrylate) and poly (PEGA-co-BMA) were synthesized and then coated on a surface of PU, followed by immobilizing CD34 mAb. $^1H$-NMR analysis demonstrated that poly(PEGA-co-BMA) copolymers with a desired composition were synthesized. Poly(PEGA-co-BMA)-coated PU was much more effective for the immobilization of CD34 mAb, comparing with PEG-grafted PU prepared in our previous study, as demonstrated by that surface density and activity of CD34 mAb increased over 32 times. Physico-chemical properties of modified PU surfaces were characterized by X-ray photoelectron spectroscopy (XPS), water contact angle, and atomic force microscopy (AFM). The results demonstrated that the poly(PEGA-co-BMA) coating was effective for CD34 mAb immobilization and feasible for applying to cardiovascular biomaterials.

Changes of Ginsenoside Content by Mushroom Mycelial Fermentation in Red Ginseng Extract

  • Bae, Song-Hwan;Lee, Hyun-Sun;Kim, Mi-Ryung;Kim, Sun-Young;Kim, Jin-Man;Suh, Hyung-Joo
    • Journal of Ginseng Research
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    • v.35 no.2
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    • pp.235-242
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    • 2011
  • To obtain microorganisms for the microbial conversion of ginsenosides in red ginseng extract (RGE), mushroom mycelia were used for the fermentation of RGE. After fermentation, total sugar contents and polyohenol contents of the RGEs fermented with various mushrooms were not a significant increase between RGE and the ferments. But uronic acid content was relatively higher in the fermented RGEs cultured with Lentus edodes (2155.6 ${\mu}g/mL$), Phelllinus linteus (1690.9 ${\mu}g/mL$) and Inonotus obliquus 26137 and 26147 (1549.5 and 1670.7 ${\mu}g/mL$) compared to the RGE (1307.1 ${\mu}g/mL$). The RGEs fermented by Ph. linteus, Cordyceps militaris, and Grifola frondosa showed particularly high levels of total ginsenosides (20018.1, 17501.6, and 16267.0 ${\mu}g/mL$, respectively). The ferments with C. militaris (6974.2 ${\mu}g/mL$), Ph. linteus (9109.2 ${\mu}g/mL$), and G. frondosa (7023.0 ${\mu}g/mL$) also showed high levels of metabolites (sum of compound K, $Rh_1$, $Rg_5$, $Rk_1$, $Rg_3$, and $Rg_2$) compared to RGE (3615.9 ${\mu}g/mL$). Among four different RGE concentrations examined, a 20 brix concentration of RGE was favorable for the fermentation of Ph. linteus. Maximum biotransformation of ginsneoside metabolites (9395.5 ${\mu}g/mL$) was obtained after 5 days fermentation with Ph. linteus. Maximum mycelial growth of 2.6 mg/mL was achieved at 9 days, in which growth was not significantly different during 5 to 9 days fermentation. During fermentation of RGE by Ph. linteus in a 7 L fermenter, $Rg_3$, $Rg_5$, and $Rk_1$ contents showed maximum concentrations after 5 days similar to flask fermentation. These results confirm that fermentation with Ph. linteus is very useful for preparing minor ginsenoside metabolites while being safe for foods.

Cholesterol-Lowering Effect of Pine Nut in Plasma of Rats (잣 성분의 혈중 콜레스테롤 저하효과)

  • Park, Young-Seo;Chung, Myong-Soo
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.702-708
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    • 2005
  • Cholesterol-lowering effect of pine nut on mice was investigated by examining changes in body weight, feed intake, and triglyceride, total cholesterol, high density liporotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and very low density lipoprotein cholesterol (VLDL-C) contents in mice plasma. Changes in body weight and feed intake between pre-designed experimental animal groups fed various contents of cholesterol and pine nut and control group fed basal diet were not significantly different. Food efficiency ratio of experimental groups fed higher than 10% pine nut was significantly higher than that of control group. Apparent differences in atherogenic index representing cholesterol-lowering effect in plasma were not found among all groups fed 0-20% pine nut. Inhibition activities of water and hexane extracts of pine nut on HMG-CoA reductase and cholesterol esterase were examined in vitro. Hexane extract showed 66% inhibition effect on HMG-CoA reductase, whereas none was observed with water extract.

Evaluation of in-vitro Anti-thrombosis and Anti-oxidation Activity of Lees of Takju (Wookukseng) (우국생 탁주 주박의 항혈전 및 항산화 활성)

  • Kim, Mi-Sun;Lee, Ye-Seul;Kim, Jong Sik;Shin, Woo-Chang;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.25 no.4
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    • pp.425-432
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    • 2015
  • This study was performed to develop high-value-added biomaterials for health and beauty products. Extracts of ethanol and hot water and their subsequent organic solvent fractions were prepared from Lees of Wookukseng (LW), a commercialized Korean traditional rice wine. We investigated their activities on blood coagulation, platelet aggregation, hemolysis against human red blood cells (hRBCs), and anti-oxidation. The water content, pH and brix of the LW were 80.3%, 3.94 and 13.0°, respectively. The yield of ethanol extraction (6.62%) was 3.15 times higher than that of hot-water extraction (2.1%), and the ethyacetate fraction (EAF) of ethanol extract showed the highest content of total polyphenol (128 mg/g) among the various fractions. In anticoagulation activity assay, the EAF of ethanol extract showed a 15-fold extension in TT, PT, and aPTT, indicating that the EAFs contain various inhibitory substances against thrombin, prothrombin and coagulation factors. In anti-platelet aggregation activity assay, the butanol fraction and water residue of ethanol extract showed significant inhibition activity. The activities were comparable to aspirin, a commercial anti-thrombosis agent. The above extracts and fractions did not show hemolysis activity against hRBC up to 5 mg/ml, and had radical scavenging activity against DPPH anion, ABTS cation and nitrite. Our results suggest that the active fractions prepared from LW, which has no specific usage until now, have a high potential as novel resources for anti-thrombosis agents.

Plasma Surface Modification of Graphene and Combination with Bacteria Cellulose (Graphene의 플라즈마 표면 개질과 박테리아 셀룰로오스와의 결합성 검토)

  • Yim, Eun-Chae;Kim, Seong-Jun;Oh, Il-Kwon;Kee, Chang-Doo
    • Korean Chemical Engineering Research
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    • v.51 no.3
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    • pp.388-393
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    • 2013
  • The study was focused to evaluate the possibility for combination membrane of bacterial cellulose (BC) and graphene with high electrical properties. BC with natural polymer matrix was known to have strong physical strength. For the combination of graphene with BC, the surface of graphene was modified with oxygen plasma by changing strength and time of radio waves in room temperature. Water contact angle of modified graphene grew smaller from $130^{\circ}$ to $12^{\circ}$. XPS analysis showed that oxygen content after treatment increased from 2.99 to 10.98%. Damage degree of graphene was examined from $I_D/I_G$ ratio of Raman analysis. $I_D/I_G$ ratio of non-treated graphene (NTG) was 0.11, and 0.36 to 0.43 in plasma treated graphene (PTG), increasing structural defects of PTG. XRD analysis of PTG membrane with BC was $2{\theta}$ same to BC only, indicating chemically combined membrane. In FT-IR analysis, 1,000 to 1,300 $cm^{-1}$ (C=O) peak indicating oxygen radicals in PTG membrane had formed was larger than NTG membrane. The results suggest that BC as an alternation of plastic material for graphene combination has a possibility in some degree on the part like transparent conductive films.

A Research on the Department Satisfaction of Department of Dental Technology Students (치기공과 학생들의 학과만족도에 관한 조사)

  • Choi, Un-Jae;Yu, Sang-Hui
    • Journal of Technologic Dentistry
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    • v.34 no.3
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    • pp.263-271
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    • 2012
  • Purpose: The purpose of this research was to examine department satisfaction for improving the competitiveness of department of dental technology and providing basic data for students successful guidance. Methods: The study subject were 560 students from 4 colleges, and they were selected by the convenient sampling method. Data for this survey was obtain by self-administration method. This questionnaire consist of general characteristics, the awareness of before entering department of dental technology, the satisfaction of department of dental technology, an opinion about change of one's course and temporary absence. Results: About the time of selecting their major, 70.1% of students answered the most before highschool. Department of dental technology and the most common source of information in selecting a majors is the person oneself(52.6%). The person who answers because of knowing a little bit for the question asking the priori knowledge of department of dental technology showed up as 66.3%. About the motivation for selection their major, 37.4% of students answered the most it seemed to be a future prospect. As to the extend of department satisfaction, the satisfaction showed up as 63.3%, but the extend of satisfaction was exposed to reduce as the grade increased. The dissatisfaction factor is the difficulty of the theory(29.8%). Many students do not want to change their majors even if they are provided with the chance to do so(68.7%). From the survey related to the experience of thinking over temporary absence and drop out, 56.8% of students responded they had experienced thinking about that. Current feelings after department selection was found to be the most significant factor that affects satisfaction of department of dental technology(Adjusted $R^2$=48.3%). Conclusion: The extend of satisfaction of the women is low. The extend of satisfaction reduces as the grade increases. The school subject extend of satisfaction can know that ratio about of considering the leave of absence and voluntary withdrawal increases. Therefore, the plan of education that women can adapt the women o the school subject well and that it can be satisfied is sought for. It is considered that effort promoting the extend of satisfaction is needed as the grade increases.