• Journal of Life Science
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• v.25 no.7
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• pp.757-764
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• 2015

The immature fruit of Rubus coreanus Miquel (bokbunja in Korean) is mainly consumed as a fruit wine due to its sour taste and low sugar content. The lees (LBW) remaining after the production of bokbunja wine are discarded as they have no specific usage. The aim of this study was to develop high-value-added biomaterials for functional foods and beauty/health products by investigating the anti-microbial, anti-oxidant, and anti-thrombosis activities of LBW using ethanol and hot water extracts and their subsequent organic solvent fractions. The ethyl acetate (EA) fraction of LBW extracts has a high polyphenol content (413–459 mg/g), and showed strong anti-microbial activity against gram-positive bacteria. The EA fraction also showed excellent radical-scavenging activity against DPPH anion, ABTS cation, and nitrite, with strong reducing power. The polyphenol-enriched EA fraction strongly inhibited thrombin, prothrombin, and blood coagulation factors. The butanol fraction showed a specific inhibition of coagulation factors, as measured in activated partial thromboplastin time assay, which is linked to intrinsic blood coagulation. The butanol fraction also showed strong inhibition of platelet aggregation, at levels comparable to aspirin. The residue of the hot-water extract, which is produced by sequential solvent fractionation of the LBW extract, showed superior inhibition against platelet aggregation when compared to aspirin. Our results suggest that the LBW, which are currently discarded, are a promising source of novel functional foods and beauty/health products.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.144-151
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• 2002

For elucidating the relationship between the biosynthetic pathways for polyhydroxyslkanoates (PHAs) and 5-aminolevulinic acid (ALA), culture conditions for the production of these two biomaterials by Rhodopseudomonas sp. KCTC 1437 were investigated. Of the carbon substrates tested, acetic acid was the best carbon source for cell growth and PHA biosynthesis. When succinic acid was added as a co-substrate into culture medium, cell growth and PHA production were greatly increased up to 2.5 g/ι and 73% of dry cell weight, respectively. The PHA obtained from the carbon substrates tested was homopolyester of 3-hydroxybutyrate, while valeric acid was only effective for the production of copolyester consisting of 3-hydroxybutyrate and 3-hydroxyvalerate. Anaerobic light culture condition was better for PHA production and cell growth than anaerobic dark or aerobic dark culture condition. The organism was capable of synthesizing ALA when glycine and succinic acid were added to the culture medium. ALA was produced to ca.400 mg/ι when levulinic acid, soccinic acid, and glycine were repeatedly added with a reductant (sodim thioglycolate). However, the presence of glycine, levulinic acid and sodium glycolate inhibited the cell growth and the conversion of carbon substrates to PHA. From these results it is apparent that the production yields of PHA and ALA could not be increased simultaneously because the optimal conditions for the production of PHA and ALA are opposed to each other.

• The Journal of Korean Medicine
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• v.28 no.1 s.69
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• pp.105-116
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• 2007

Objectives : Mahuang (Ephedra sinica), well known as an herbal medicine in the East and West, contains a relatively high percentage of ephedrine known as sympathomimetic alkaloid. We investigated the effects of Mahuang on sympathetic nervous system with heart rate variety (HRV). Time and frequency domain analysis of HRV is a noninvasive technique capable of providing information on autonomic modulation of the sinus node. Methods : We investigated 57 healthy volunteers consisting of 37 subjects in the Mahuang group and 20 subjects in the placebo group. Study form was a randomized, placebo-controlled, double-blind clinical trial. The 37 subjects in the Mahuang group took 3 Mahuang capsules (1 capsule equivalent to 2g herb Mahuang) twice a day at 10 a.m. and 2 p.,., while the 20 subjects in the placebo group took 3 placebo-capsules filled with glutinous rice powder at the same times. Mahuang medicine and placebo medicine were made into opaque capsules. We measured HRV at 3 p.m. 1 or 2 days before medication and at 3 p.m. after medication. Results : Mean-RR and SDNN of the Mahuang group significantly decreased compared with that of the placebo group, but the heart rate of the Mahuang group significantly increased compared with the placebo. HRV-Index, RMSSD and SDSD of the Mahuang group significantly decreased compared with that of the placebo group, but PNN50 of the Mahuang group significantly increased compared with placebo. Ln(TP), Ln(VLF), Ln(LF) and Ln(HF) of the Mahuang group significantly decreased compared with those of the placebo group. There were no significant differences in normalized LF, normalized HF and LF/HF ratio between the Mahuang and placebo groups. Conclusion : The results suggest that Mahuang in healthy adults tends to reduce the autonomic nervous system within the normal range.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.545-554
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• 2013

Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,126 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative replication protein similar to rolling circular replication proteins from other lactic acid bacteria. However, a single-stranded intermediate was not detected when S1 nuclease was treated, suggesting it may follow theta replication. Interestingly, the replication initiation site of this plasmid is 100% identical to other plasmids from lactic acid bacteria, suggesting it may function for replication initiation. To construct a surface layer expression vector, pTSLGFP, slpA encoding the surface layer protein from Lactobacillus acidophilus was PCR amplified and fused with the gfp gene, forming a SLGFP fused gene. The plasmid pKW2124 was cloned into the XbaI site of pUC19, forming an Weissella-E. coli shuttle vector pKUW22. NheI-linearized pTSLGFP was ligated into pKUWCAT containing pKUW22 and the chloramphenicol acetyltransferase gene from pEK104, resulting in an 8.6 kb pKWCSLGFP surface layer expression vector. After transformation of this vector into W. cibaria KLC140, a GFP fluorescence signal was detected on the surface of the transformant, substantiating production of SLGFP fused protein and its secretion. This is the first report for construction of a Weissella surface layer expression vector, which may be useful for surface layer production of beneficial proteins in Weissella.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.39 no.4
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• pp.303-311
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• 2013

This study was performed to investigate the anti-inflammatory activities of fermented Scutellaria baicalensis extracts using Lactobacillus rhamnosus. The extracts were WE (water extract at $100^{\circ}C$ for 24 h), EE (70% ethanol extract at $60^{\circ}C$ for 24 h), FWE (fermented and water extract at $60^{\circ}C$ for 24 h), FEE (fermented and 70% ethanol extract at $60^{\circ}C$ for 24 h). The cytotoxicity of the extracts was in the range of 11.2 ~ 15.6 % at 1.0 mg/mL concentratioin. The FEE showed the lowest activity at 1.0 mg/mL concentratioin. Compared to the WE, hyaluronidase inhibitory activity contents in the FEE increased to 9.2% at 1.0 mg/mL concentratioin. Nitric oxide production of WE, EE, FWE and FEE at 1.0 mg/mL concentration was mesured as 7.6, 7.9, 6.9, 6.4 ${\mu}M$, respectively. $PGE_2$ secretion of the human fibroblast of the FEE were lower than 810 pg/mL. Our results suggested that the extracts from fermentation process after 70% ethanol extraction had relatively high anti-inflammatory activities and that the Scutellaria baicalensis could be more extracted in FEE than others.

• Journal of the Korean Recycled Construction Resources Institute
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• v.3 no.4
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• pp.348-354
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• 2015

Urethane waterproofing materials which currently being used most commonly in the Korean domestic market have high applicability and construct layers without forming any joints, but under the influence of low temperature and low humidity, as well as the thickness of the applied layer, the curing time of this material may become extended in construction sites. To resolve these issues, a proposed method of using water-hardening type of polyurethane waterproofing materials are being developed. However, there currently lacks any standards or evaluation methods on determining an optimal mixture ratio of water for the water-hardening polyurethane waterproofing materials. Therefore, workers and construction workers are relying on their hands-on-skills and experience to determine this ratio and are not able to obtain a consistent performance quality out of these materials. Therefore, for the establishment of a board applicability of the water-hardening polyurethane waterproofing methods in construction sites, this study objectively analyzes the changes in the performance of these materials depending on the changes of the water mixture ratio and attempts to procure the optimal ratio on the basis of forming a provisionary standard. The study prepares mixtures of varying ratio(no water added, 5, 10, 15, 20, 25, 30, 40 and 50) and conducts comparative analysis respective mixtures' performances and their changes in the physical properties in an objective manner and proposes a provisional optimal value that can serve as a data base that can be used as a reference for future studies.

• Journal of the Korean Recycled Construction Resources Institute
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• v.3 no.3
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• pp.212-219
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• 2015

This study focuses evaluating the efficiency and performance evaluation of composite type sheet-membrane waterproofing method that utilizes a separation behavior inducement system designed to resolve the chronic problems of disintegration and damage of overlap areas of waterproofing layers. As the result of the test, the tensile strength value was at 13.8N/mm and elongation rate at 587% for the separation behavior inducement type specimen, and the compared specimens had 14.2N/mm for tensile strength and 335% for elongation rate. For the separation behavior adhesion method specimen, when tensile stress or displacement occurred, the Zero-Span tension occurrence did not follow, which resulted in that the bottom sheet layer and the top membrane layer did not simultaneously becoming damaged. When undergoing the top and bottom layers were separated through separation behavior due to lack of flexibility, the bottom layer began to damage at the primary stage, and with the allowed boundary the upper membrane layer began to display flexibility and showed continuous displaced resulting in secondary phase damaging.

• Journal of the Korean Recycled Construction Resources Institute
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• v.4 no.4
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• pp.379-387
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• 2016

In this study, a survey was conducted on the required measurement and waterproofing performance for residential building underground structure leakage prevention system. According to the results of the survey, it has been determined that leakage problem is considered to be quite severe, and many respondents have agreed. The legal guidelines and regulation systems do not reflect properly on the environmental requirements or conditions, resulting in continued leakage problem. In regards to this, a standardized waterproofing technique that can be used in underground areas of residential structures is required as an obligation and the development of high performance waterproofing method that allows for wet concrete surface adhesion and a guideline, design and maintenance method that allows the control of overall situational control of leakage is required.

• The korean journal of orthodontics
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• v.40 no.3
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• pp.167-175
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• 2010

Objective: The aim of this study was to validate the Periotest values for the prediction of orthodontic mini-implants' stability. Methods: Sixty orthodontic mini-implants (7.0 mm $\times$ $\emptyset1.45$ mm; ACR, Biomaterials Korea, Seoul, Korea) were inserted into the buccal alveolar bone of 5 twelve month-old beagle dogs. Insertion torque (IT) and Periotest values (PTV) were measured at the installation procedure, and removal torque (RT) and PTV were recorded after 12 weeks of orthodontic loading. To correlate PTV with variables, the cortical bone thickness (mm) and bone mineral density (BMD) within the cortical bone and total bone area were calculated with the help of CT scanning. Results: The BMD and cortical bone thickness in mandibular alveolus were significantly higher than those of the maxilla (p < 0.05). The PTV values ranged from -3.2 to 4.8 for 12 weeks of loading showing clinically stable mini-implants. PTV at insertion was significantly correlated with IT (-0.51), bone density (-0.48), cortical bone thickness (-0.42) (p < 0.05) in the mandible, but showed no correlation in the maxilla. PTV before removal was significantly correlated with RT (-0.66) (p < 0.01) in the mandible. Conclusions: These results show that the periotest is a useful method for the evaluation of mini-implant stability, but it can only be applied to limited areas with thick cortical and high density bone such as the mandible.

• The Journal of Korean Academy of Prosthodontics
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• v.43 no.2
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• pp.204-217
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• 2005

Purpose. The purpose of this study was to analyze stress distribution of all ceramic posterior fixed partial denture using a three dimensional finite element method. Material and method. A three dimensional finite element model was created to demonstrate all-ceramic posterior fixed partial denture and then this computer model measured the stress distribution of the all ceramic bridges which has a ceramic core materials such as Zirconia, IPS Empress. 2. In-Ceram zirconia, Metal-Ceramic. Also the stress distribution was examined according to loading sites when force was applied to sites such as the central area of second premolar the mesial connector of pontic, the central fossa of pontic, the distal connector of pontic, and the central fossa of second molar. Results. 1. In all the materials of the core in this study, von Mises stress indicated that the stress increased as force was applied to loaded sites, just at those points, on the connector, and the margin in the area adjacent to the connectors. 2. The maximum principal stress was much higher in the lower part of the connectors than in any other region. 3. As the load was applied to the different locations, the research showed a consistent increase of stress in the lower connectors. The maximum value of the von Mises stress was two or three times greater when the load was applied directly to the connectors rather than indirectly through another stressed region. 4. In the case of In-Ceram zirconia, the stress in lower connectors was the highest of all the reference points, the stress showed 75% of all the maximum stress. Ziconia showed 72%, Metal Ceramic 67% and IPS Empress 2 50%. 5. In the case of Ziconia, the stress was well dispersed in each reference point that the stress differences were smaller when compared to In-Cream ziconia.