• Title/Summary/Keyword: biomaterials

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Neuroprotectuve Effect of Homosyringaldehyde Isolated from Cynanchum panuculatum against Glutamate-Induced Neurotoxicity (서장경으로부터 분리한 Homosyringaldehyde의 뇌신경세포 보호 활성)

  • Weon, Jin Bae;Lee, Bohyoung;Yun, Bo-Ra;Lee, Jiwoo;Ma, Choong Je
    • YAKHAK HOEJI
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    • v.56 no.5
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    • pp.299-303
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    • 2012
  • Homosyringaldehyde was isolated and identified from the 80% methanol extract of roots of Cynanchum paniculatum. C. paniculatum has been widely used for the treatment of various diseases such as neurasthenia, insomnia, dysmenorrheal and toothache. This compound exerted significant neuroprotective activities against glutamate-induced neurotoxicity in hippocampal HT22 cell line by 37.53% (at the concentration of $100{\mu}M$). We investigated mode of action of this compound. Homosyringaldehyde ($100{\mu}M$) significantly decreased the ROS level and $Ca^{2+}$ concentration in the oxidative stress induced HT22 cells by oxidative glutamate toxicity. Thus, our results suggest that homosyringaldehyde significantly protect HT22 cells against glutamate-induced oxidative stress, via antioxidative activities. As the results, we suggest that homosyringaldehyde may be useful in the treatment of neurogenerative disorders.

Enhancement of Bioactivity of Sosihotang with Steaming Process (증숙에 따른 소시호탕의 활성 변화)

  • Weon, Jin-Bae;Ma, Jin-Yeul;Yang, Hye-Jin;Lee, Bo-Hyoung;Yun, Bo-Ra;Ma, Choong-Je
    • Korean Journal of Pharmacognosy
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    • v.42 no.3
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    • pp.271-275
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    • 2011
  • Sosihotang is a herbal medicine traditionally used to treat chronic liver disease. This study was performed to investigate the effect of steaming Sosihotang at a $121^{\circ}C$ on its major compounds and neuroprotective activity. We evaluated quantitative changes of 6 major compounds in Soshihotang, saikosaponin A, homogentisic acid, baicalin, ginsenoside $Rg_3$, glycyrrhizin and 6-gingerol using HPLC-DAD. After steaming produce, saikosaponin A, homogentisic acid, baicalin, glycyrrhizin and 6-gingerol in Sosihotang were decreased and ginsenoside $Rg_3$ was increased. The neuroprotective activity of steamed Sosihotang was determined by MTT assay, comparing to Sosihotang. The steamed sosihotang showed a higher neuroprotective activity than Sosihotang. In conclusion, steam produce could improve neuroprotective activity of Soshihotang.

Quantitative Analysis of Aucklandia Lappa Using Costunolide and Dehydrocostuslactone (Costunolide와 Dehydrocostuslactone을 이용한 목향의 함량분석)

  • Eom, Min Rye;Weon, Jin Bae;Yun, Bo-Ra;Lee, Jiwoo;Ma, Choong Je
    • Korean Journal of Pharmacognosy
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    • v.44 no.3
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    • pp.235-241
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    • 2013
  • Aucklandia lappa Decne (Compositae) has been used for treatment of abdominal pain, vomiting, diarrhea, chronic inflammation, and antibacterial effect. The quality of these herbs has been affected by many factors such as collection time, place, temperature, cultivation environment and manufacturing process. We used costunolide and dehydrocostuslactone as marker compounds for quality evaluation of rhizome of Aucklandia lappa. 66 samples of Aucklandia lappa were collected from those habitats in Korea and China. The developed HPLC-DAD method was applied to investigate for quality control of Aucklandia lappa samples. The average contents of the costunolide and dehydrocostuslactone were 2.3895% and 0.9258%, respectively. The principal component analysis (PCA) exhibited that classification of Aucklandia lappa according to origin not separated. Results of this study may be satisfactory applied to determination of content criteria of Aucklandia lappa.

Low Cariogenicity of Maltosyl-erythritol, Major Transglycosylation Product of Erythritol, by Bacillus stearothermophilus Maltogenic Amylase

  • Jeon, Eun-Joo;Jung, Il-Hun;Cho, Kil-Soon;Seo, Eun-Sung;Kim, Do-Man;Lee, Sung-Joon;Park, Kwan-Hwa;Moon, Tae-Wha
    • Journal of Microbiology and Biotechnology
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    • v.13 no.5
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    • pp.815-818
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    • 2003
  • Maltosyl(G2)-erythritol, produced by the transglycosylation reaction of erythritol with maltotriose by Bacillus stearothermophilus maltogenic amylase, was not utilized either as a substrate for lactic acid production or for water-insoluble glucan synthesis. An inhibition assay of dextransucrase and mutansucrase showed that the dental caries suppression effect of G2-erythritol was greater than that of erythritol.

Quantitative Analysis for the Quality Evaluation of Sinomenine, Magnoflorine and Syringaresinol in Sinomenium acutum (방기의 품질 평가를 위한 Sinomenine, Magnoflorine, Syringaresinol의 함량 분석)

  • Lee, Jiwoo;Weon, Jin Bae;Yun, Bo-Ra;Eom, Min Rye;Ma, Choong Je
    • YAKHAK HOEJI
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    • v.57 no.3
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    • pp.161-166
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    • 2013
  • Sinomenium acutum has been used for the treatment of rheumatoid arthritis, arrhythmia, and pain. We evaluated the quality of S. acutum extract by quantitatively analyzing its components such as sinomenine, magnoflorine and syringaresinol with the simultaneous determination method using HPLC-DAD. A total of 53 samples collected from different localities were evaluated with this quality evaluation method. Sinomenine, magnoflorine and syringaresinol from tested samples ranged from 0.0649~9.1050%, 0.7460~10.7590% and 0.0010~0.2441%, respectively. In the current study, we were able to exhibit the diverse quality of S. acutum samples collected from various locations using the simultaneous determination method.

Electrospun Zein Nanofibrous Membrane for Chromium(VI) Adsorption (제인 나노섬유 멤브레인을 이용한 6가 크롬 이온 흡착 평가)

  • Yun, Na Kyong;Kwak, Hyo Won;Lee, Min Gyu;Lee, Seo Kyung;Lee, Ki Hoon
    • Textile Science and Engineering
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    • v.51 no.2
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    • pp.57-62
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    • 2014
  • Heavy metal pollution is a serious problem that harms the environment and human health. As a means of removing heavy metal ions, especially Cr(VI), from a solution, we prepared a nanofibrous membrane from zein protein which is derived from maize. The zein nanofibrous membrane (ZNM) was prepared by using an 80% ethanol solution as a solvent. The resulting fiber had a ribbon-like structure. The size of the nanofiber could be controlled by varying the concentration of the zein in the ethanol solution. The ZNM was mounted in a membrane holder and a Cr(VI) feed solution was then continuously passed through the ZNM. The adsorption of Cr(VI) reached equilibrium within 3 min, indicating the rapid adsorption of the Cr(VI). To regenerate the ZNM adsorbent, the adsorbed Cr(VI) was removed by adding 0.1 M NaOH. We believe that ZNM would be an effective means of removing Cr(VI) from industrial waste.

Expression and Purification of Ubiquitin-Specific Protease (UBP1) of Saccharomyces cerevisiae in Recombinant Escherichia Coli

  • Na, Kang-In;Kim, Myoung-Dong;Min, Won-Ki;Kim, Jeong-Ah;Lee, Woo-Jong;Kim, Dae-Ok;Park, Kyung-Moon;Seo, Jin-Ho
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.599-602
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    • 2005
  • Truncated form of UBP1, an ubiquitin-specific protease of Saccharomyces cerevisiae, was overexpressed in Escherichia coli. The hexahistidine residue $(His_6)$ was fused to the N-terminus of truncated UBP1 and the corresponding recombinant protein was purified with high yield by immobilized metal affinity chromatography. The truncated form of UBP1 protein was functional to cleave ubiquitinated human growth hormone as substrate. Effects of pH and temperature were investigated in order to optimize deubiquitinating reactions for the truncated UBP1. Optimum temperature and pH for the cleavage reaction were $40^{\circ}C$ and pH 8.0, respectively.

Vibration Analysis of Gearbox for Agricultural UTV using a Reduced-Order Model (축소 모델 기법을 이용한 농업용 전동식 동력운반차 감속기의 진동 분석)

  • Kim, Beom-Soo;Cho, Seung-Je;Shin, In-Kyung;Chung, Woo-Jin;Han, Hyun-Woo;Kim, Ji-Tae;Park, Young-Jun
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.18 no.8
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    • pp.8-17
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    • 2019
  • In this study, a model reduction technique was used to develop a precise noise and vibration prediction model for the individual components of a driveline system. The dynamic reduced-order model generated by the Craig-Bampton method was applied to perform dynamic analysis of an electric agricultural power cart. The natural frequency and acceleration response results were analyzed according to the different number of dominant sub-structural modes contained in the reduced-order models. Through the analysis results, it was confirmed that a sufficient number of dominant sub-structures to satisfy the operating conditions should be selected to construct an optimal reduced-order model.

Acetate decreases PVR/CD155 expression via PI3K/AKT pathway in cancer cells

  • Tran, Na Ly;Lee, In Kyu;Choi, Jungkyun;Kim, Sang-Heon;Oh, Seung Ja
    • BMB Reports
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    • v.54 no.8
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    • pp.431-436
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    • 2021
  • In recent years, restoring anti-tumor immunity has garnered a growing interest in cancer treatment. As potential therapeutics, immune checkpoint inhibitors have demonstrated benefits in many clinical studies. Although various methods have been applied to suppress immune checkpoints to boost anti-tumor immunity, including the use of immune checkpoint inhibitors, there are still unmet clinical needs to improve the response rate of cancer treatment. Here, we show that acetate can suppress the expression of poliovirus receptor (PVR/CD155), a ligand for immune checkpoint, in colon cancer cells. We demonstrated that acetate treatment could enhance effector responses of CD8+ T cells by decreasing the expression of PVR/CD155 in cancer cells. We also found that acetate could reduce the expression of PVR/CD155 by deactivating the PI3K/AKT pathway. These results demonstrate that acetate-mediated expression of PVR/CD155 in cancer cells might potentiate the anti-tumor immunity in the microenvironment of cancer. Our findings indicate that maintaining particular acetate concentrations could be a complementary strategy in current cancer treatment.

Biodegradable sheath-core biphasic monofilament braided stent for bio-functional treatment of esophageal strictures

  • Han, Cheol-Min;Lih, Eugene;Choi, Seul-Ki;Bedair, Tarek M.;Lee, Young-Jae;Park, Wooram;Han, Dong Keun;Son, Jun Sik;Joung, Yoon Ki
    • Journal of Industrial and Engineering Chemistry
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    • v.67
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    • pp.396-406
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    • 2018
  • In this study, a polydioxanone (PDO) and poly(L-lactic acid) (PLLA) sheath-core biphasic monofilament was designed to develop an esophageal stent with improved mechanical properties and controlled biodegradability. The radial force of PDO/PLLA sheath-core stent was 10.24 N, while that of PDO stent was 5.64 N. Deteriorations of tensile strength, elastic modulus and elongation during degradation test were also delayed on PDO/PLLA group. Hyaluronic acid-dopamine conjugate and $BaSO_4/PDO$ conjugate coating layers provided improved tissue adhesion strength and reasonable X-ray contrast, respectively. Taken all together, the sheath-core filaments with tissue adhesive and radiopaque properties will be useful in designing esophageal stents.