• Journal of Microbiology and Biotechnology
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• v.33 no.5
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• pp.687-697
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• 2023

Identification of novel, electricity-producing bacteria has garnered remarkable interest because of the various applications of electricigens in microbial fuel cell and bioelectrochemical systems. Shewanella marisflavi BBL25, an electricity-generating microorganism, uses various carbon sources and shows broader sugar utilization than the better-known S. oneidensis MR-1. To determine the sugar-utilizing genes and electricity production and transfer system in S. marisflavi BBL25, we performed an in-depth analysis using whole-genome sequencing. We identified various genes associated with carbon source utilization and the electron transfer system, similar to those of S. oneidensis MR-1. In addition, we identified genes related to hydrogen production systems in S. marisflavi BBL25, which were different from those in S. oneidensis MR-1. When we cultured S. marisflavi BBL25 under anaerobic conditions, the strain produced 427.58 ± 5.85 µl of biohydrogen from pyruvate and 877.43 ± 28.53 µl from xylose. As S. oneidensis MR-1 could not utilize glucose well, we introduced the glk gene from S. marisflavi BBL25 into S. oneidensis MR-1, resulting in a 117.35% increase in growth and a 17.64% increase in glucose consumption. The results of S. marisflavi BBL25 genome sequencing aided in the understanding of sugar utilization, electron transfer systems, and hydrogen production systems in other Shewanella species.

• Proceedings of the IEEK Conference
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• 2003.07c
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• pp.2911-2914
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• 2003

Conventionally, biological manipulations have been performed manually with long training and pretty low success rates. To overcome this problem, a novel biological manipulation system has been developed to manipulate biological cells without any interference of a human operator, In this paper, we demonstrate a development of tole-autonomous Cell Manipulation System (CMS) using an image processing at a remote site. The CMS consists of two manipulators, a plane stage, and an optical microscope. We developed deformable template-model-matching algorithm for micro objects and pattern matching algorithm of end effect for these manipulators in order to control manipulators and the stage. Through manipulation of biological cells using these algorithms, the performance of the CMS is verified experimentally.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.281-286
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• 2005

Biofiltration is a biological process which is considered to be one of the more successful examples of biotechnological applications to environmental engineering, and is most commonly used in the removal of odoriferous compounds. In this study, we have attempted to assess the efficiency with which both single and complex odoriferous compounds could be removed, using one- or two-stage biofiltration systems. The complex gas removal scheme was applied with a 200 ppm inlet concentration of ethanol, 70 ppm of acetaldehyde, and 70 ppm of toluene with EBCT for 45 seconds in a one- or two-stage biofiltration system. The removal yield of toluene was determined to be lower than that of the other gases in the one-stage biofilter. Otherwise, the complex gases were sufficiently eliminated by the two-stage biofiltration system.

• BMB Reports
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• v.31 no.5
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• pp.503-507
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• 1998

To investigate the effects of the Vitreoscilla hemoglobin (VHb) gene on the production of a heterologous protein, a comparative expression system for VHb and ferritin was constructed. First, the VHb gene was inserted into the downstream and upstream regions of the ferritin gene to construct pHF2 and pHF3, respectively. Next, the two plasmids pACHB1 and pVUTFH10, having the VHb gene and the ferritin gene respectively, were constructed in order to express the two genes in different plasmids by using a coplasmid expression system. It was observed that the cell growth was improved in all strains containing the VHb gene. Furthermore, in our coplasmid expression system, the presence of the VHb gene increased production of the ferritin by 1.8 times, as much as that in a strain not having the VHb gene.

• Proceedings of the Korean Fiber Society Conference
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• 2007.11a
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• pp.297-298
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• 2007

• Proceedings of the Korean Fiber Society Conference
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• 2007.11a
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• pp.405-406
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• 2007

• Smart Structures and Systems
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• v.8 no.1
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• pp.107-117
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• 2011

Biological sensory systems continuously monitor and analyze changes in real-world environments that are relevant to an animal's specific behavioral needs and goals. Understanding the sensory mechanisms and information processing principles that biological systems utilize for efficient sensory data acquisition may provide useful guidance for the design of smart-sensing systems in engineering applications. Weakly electric fish, which use self-generated electrical energy to actively sense their environment, provide an excellent model system for studying biological principles of sensory data acquisition. The electrosensory system enables these fish to hunt and navigate at night without the use of visual cues. To achieve reliable, real-time task performance, the electrosensory system implements a number of smart sensing strategies, including efficient stimulus encoding, multi-scale virtual sensor arrays, task-dependent filtering and online subtraction of sensory expectation.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.3 no.1
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• pp.75-80
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• 1999

An investigation of noise and vibration caused by the reclamation of the foreshore around J village resulted in noise measurements of 56-84 dB at the first point, 62-81 dB at the second point, and 68-78 dB at the third point. These measurements were higher than the standard level of environmental noise that is 55 dB at noon and 45 dB at night. The vibration measurements were 61-83 dB at the first point, 63-88 dB at the second point, and 58-77 dB at the third point. These measurements were also higher than the standard level of environmental vibration that is 60 dB at noon and 54 dB at night. The measurements of scattering dust were 80 $\mu\textrm{g}$/$\textrm{m}^3$ at the first point, 120 $\mu\textrm{g}$/$\textrm{m}^3$ at the second point, and 169 $\mu\textrm{g}$/$\textrm{m}^3$ at the third point. These measurements were lower than the standard level of environmental dust that is 300 $\mu\textrm{g}$/$\textrm{m}^3$/day. Although the maximum vibration level was higher than the standard level of environmental vibration, it had no influence on the construction.

• The Plant Pathology Journal
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• v.36 no.3
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• pp.289-296
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• 2020

Type VI secretion system (T6SS) is a contact-dependent secretion system, employed by most gram-negative bacteria for translocating effector proteins to target cells. The present study was conducted to investigate T6SS in Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight in rice, and to unveil its functions. Two T6SS clusters were found in the genome of Xoo PXO99^A. The deletion mutants, Δhcp1, Δhcp2, and Δhcp12, targeting the hcp gene in each cluster, and a double-deletion mutant targeting both genes were constructed and tested for growth rate, pathogenicity to rice, and inter-bacterial competition ability. The results indicated that hcp in T6SS-2, but not T6SS-1, was involved in bacterial virulence to rice plants. However, neither T6SS-1 nor T6SS-2 had any effect on the ability to compete with Escherichia coli or other bacterial cells. In conclusion, T6SS gene clusters in Xoo have been characterized, and its role in virulence to rice was confirmed.

• Journal of The Korean Association For Science Education
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• v.5 no.1
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• pp.11-17
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• 1985

This study had its objective to indentify the basic biological concepts as recommended by scholars in biology and by high school biology teachers for inclussion in the high school biology curriculum and to determine the importance levels of the concepts. The scholars for this study were selected to insure that their majors are evenly distributed across the biological areas. The final concept list contains 137 biological concepts along with their importance level judged by the scholars and high school biology teachers. The biological concepts are categorized into 12 biological areas. This study suggests that the concepts in the final list can not refect the conceptual hierarchy or inclusiveness, goals or objectives of biology education in the high school, and difficulty levels of the concepts. This study also claims that the concept list like the one produced by this study should be successively' checked and revised due to the continuous development of biological knowledge system and the constant change in the meanings of the knowledge system.