• Title/Summary/Keyword: biological stability

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Marine ecosystem risk assessment using a land-based marine closed mesocosm: Proposal of objective impact assessment tool (육상 기반 해양 폐쇄형 인공생태계를 활용한 해양생태계 위해성 평가: 객관적인 영향 평가 tool 제시)

  • Yoon, Sung-Jin
    • Korean Journal of Environmental Biology
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    • v.39 no.1
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    • pp.88-99
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    • 2021
  • In this study, a land-based marine closed mesocosm (LMCM) experiment was performed to objectively assess the initial stability of an artificial ecosystem experiment against biological and non-biological factors when evaluating ecosystem risk assessment. Changes in the CV (coefficient of value) amplitude were used as data to analyze the stability of the experimental system. The CV of the experimental variables in the LMCM groups (200, 400, 600, and 1,000 L) was maintained within the range of 20-30% for the abiotic variables in this study. However, the difference in CV amplitude in biological factors such as chlorophyll-a, phytoplankton, and zooplankton was high in the 600 L and 1,000 L LMCM groups. This result was interpreted as occurring due to the lack of control over biological variables at the beginning of the experiment. In addition, according to the ANOVA results, significant differences were found in biological contents such as COD (chemical oxygen demand), chlorophyll-a, phosphate, and zooplankton in the CV values between the LMCM groups(p<0.05). In this study, the stabilization of biological variables was necessary to to control and maintain the rate of changes in initial biological variables except for controllable water quality and nutrients. However, given the complexity of the eco-physiological activities of large-scale LMCMs and organisms in the experimental group, it was difficult to do. In conclusion, artificial ecosystem experiments as a scientific tool can distinguish biological and non-biological factors and compare and analyze clear endpoints. Therefore, it is deemed necessary to establish research objectives, select content that can maintain stability, and introduce standardized analysis techniques that can objectively interpret the experimental results.

ON A CERTAIN FINITE DIFFERENCE SCHEME FOR A MODEL FOR DIFFUSION OF BIOLOGICAL POPULATIONS

  • Asghar, Kerayechian
    • Journal of applied mathematics & informatics
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    • v.6 no.2
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    • pp.455-466
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    • 1999
  • In this note we present a numerical scheme for finding an approxximate solution of an equation which can be viewed as a model for spatial diffusion of age-depednent biological populations. Discretization of the model yields a linear system with a block tridi-agonal matrix. Our main concern will be discussion of stability for this scheme by examining the eigenvalues of the block tridiagonal matrix. Numerical results are presented.

Effect of protein and oil concentration on the emulsion stability of soy protein isolate (단백질과 기름농도가 분리대두단백질의 유화안정성에 미치는 영향)

  • Hwang, Jae-Kwan;Kim, Young-Sook;Pyun, Yu-Ryang
    • Applied Biological Chemistry
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    • v.35 no.6
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    • pp.457-461
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    • 1992
  • The emulsion stabilizing properties of soy protein isolate (SPI) were investigated in terms of the protein and oil concentration. Particularly, the dependence of emulsion stability on the oil particle size and viscosity of emulsion was studied in conjunction with the adsorption pattern of protein onto the water/oil interface during emulsification. The data showed that increasing protein concentration decreased the oil particle size and increased the emulsion viscosity, resulting in the enhanced emulsion stability. In contrast, increasing oil concentration increased both the oil praticle size and the emulsion viscosity, and thus emulsion stability varied depending on which factor predominated the overall emulsion system.

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Comparative Stability of vit E isomers Extracted from Unsaponifiable Fractions of Rice Bran Oil under Various Temperature and Oxygen Conditions

  • Lee, Young-Sang;Park, Soon-Ryang
    • Korean Journal of Plant Resources
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    • v.21 no.6
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    • pp.435-439
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    • 2008
  • Due to the fact that tocopherols and tocotrienols have antioxidant and anticancer properties, the commercial utilization of unsaponifiable fractions in rice bran is increasing. These nutraceutical compounds, however, are fairly unstable and readily break down when exposed to oxygen or lighting conditions. To compare the relative sensitivity of vit E isomers to heat and oxygen, concentrated unsaponifiable fractions extracted from crude rice bran oil were exposed to various temperature, oxygen (nitrogen-balanced), and bathing solvent conditions and resultant concentration changes in ${\alpha}$- and ${\gamma}$-tocopherols (T) and tocotrienols (T3) were evaluated. Each isomer exhibited different heat stability. Among them, ${\alpha}$-T3 degraded more rapidly compared to other vit E isomers while ${\alpha}$-T was the most stable isomer. Oxygen level also showed significant impact on each isomer's stability where severe reductions of ${\gamma}$-T (by 20%) and ${\gamma}$-T3 (by 29%) were observed under 2% oxygen conditions, while under 0% oxygen conditions no degradation could be observed even after exposure to $95^{\circ}C$ for 4 hours. When various blending solvents were mixed with concentrated unsaponifiable fractions, organic solvents such as isooctane and hexane were more effective in maintaining the stability of ${\gamma}$- T3 compared to edible oils, among which com oil was more efficient than soybean and rice bran oils.

A Study on the oxidation characteristics of micro-algal bio diesel derived from Dunaliella tertiolecta LB999 (Dunaliella tertiolecta LB999 유래 바이오디젤의 산화특성 연구)

  • Lee, Don-Min;Lee, Mi-Eun;Ha, Jong-Han;Ryu, Jin-Young;Choi, Chang-Yong;Shim, Sang-Hyuk;Lim, Sang-Min;Lee, Choul-Gyun;Lee, Bong-Hee
    • Journal of Marine Bioscience and Biotechnology
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    • v.7 no.1
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    • pp.1-10
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    • 2015
  • Bio diesel has advantages to reduce GHG(Greenhouse Gas) compare with the fossil fuel by using oil comes from plant/animal sources and even waste such as used cook oil. The diversity of energy feeds brings the positive effects to secure the national energy mix. In this circumstance, micro-algae is one of the prospective source, though some technical barriers. We analyzed the bio diesel which was derived from Dunaliella tertiolecta LB999 through the BD100 quality specifications designated by the law. From that result, it is revealed that the oxidation stability is one of the properties to be improved. In order to find the reason for low oxidation stability, we analyzed the oxidation tendency of each FAME components through some methods(EN 14111, EN14112, EN16091). In this study, we could find the higher double bond FAME portion, the more oxidative property(C18:1${\ll}C18:3$) in bio diesel and main unsaturated FAME group is acted as the key component deciding the bio diesel's oxidation stability. It is proved experimentally that C18:3 FAME are oxidized easily under the modified accelerated oxidation test. We also figure out low molecular weight hydrocarbon and FAME were founded as a result of thermal degradation. Some alcohol and aldehydes were also made by FAME oxidation. In conclusion, it is necessary to find the way to improve the micro-algal bio diesel's oxidation stability.

Effect of Casein Dephosphorylation on Stability of Casein Micelles towards Ethanol (카제인의 탈인산화가 카제인 미셀의 에탄올 안정성에 미치는 영향)

  • Shin, Weon-Sun;Moon, Tae-Wha
    • Applied Biological Chemistry
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    • v.38 no.3
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    • pp.254-258
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    • 1995
  • Various artificial casein micelle systems were prepared from dephosphorylated whole casein, ${\beta}$- or ${\kappa}$-casein and their stabilities towards ethanol were assessed. Ethanol stability was lower in the micelle systems with dephesphorylated whole casein as compared to the artificial micelles prepared from native whole casein, and the stability decreased with the extent of dephosphorylation. The casein micelles with partially dephosphorylated ${\kappa}$-casein had a lower ethanol stability than those with native ${\kappa}$-casein. Ethanol stability of the micelle system with dephosphorylated ${\beta}$-casein decreased as the degree of dephosphorylation increased. Progressive dephosphorylation of caseins in skim milk system resulted in a decrease of the stability towards ethanol. The decrease was less than that in the system with dephosphorylated individual caseins. Increase in pH of the artificial casein micelle systems in the range of $6.3{\sim}7.2$ led to an increased ethanol stability manifesting that the presence of serine phosphates contributes significantly to the stability towards ethanol.

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Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications

  • Zhang, Guoyan;An, Yingfeng;Zabed, Hossain M.;Guo, Qi;Yang, Miaomiao;Yuan, Jiao;Li, Wen;Sun, Wenjin;Qi, Xianghui
    • Journal of Microbiology and Biotechnology
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    • v.29 no.2
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    • pp.179-190
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    • 2019
  • Bacillus subtilis spore surface display (BSSD) technology is considered to be one of the most promising approaches for expressing heterologous proteins with high activity and stability. Currently, this technology is used for various purposes, such as the production of enzymes, oral vaccines, drugs and multimeric proteins, and the control of environmental pollution. This paper presents an overview of the latest developments in BSSD technology and its application in protein engineering. Finally, the major limitations of this technology and future directions for its research are discussed.

Analysis of benthic macroinvertebrates community stability and similarity in the Giran stream (길안천 저서성대형무척추동물의 군집안정성 및 유사도 분석)

  • Jang, Myeong Seong;Seo, Eul Won;Lee, Jong Eun
    • Korean Journal of Environmental Biology
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    • v.38 no.4
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    • pp.714-723
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    • 2020
  • This study was conducted to investigate the community stability and similarity of benthic macroinvertebrates in the Giran stream between August and September 2018, and compare results to those reported by Lee (2004). As relates to the total number of species in each taxon in 2018, 45 species were additionally discovered compared to the 2003 study; the number of EPT taxa increased by 14 species and OCH taxa increased by 18 species. The diversity and richness indexes increased while the dominance index tended to decrease. According to analysis of functional feeding groups, 11 more Gathering-collector species were found, making it the highest functional feeding group with 24 species. According to analysis of functional habitat groups, 15 more clinger species were found than in the past, making it the highest functional habitat group with 41 species. A community stability comparison showed that species belonging to 'Stability Group I' had the highest stability rate at 57.1% in 2003 and 61.5% in 2018. According to the biological water quality assessment, in 2018, the average water quality level at each survey site was 'Ia' and 'Very Good' in terms of environmental conditions. As a result of the similarity analysis between the survey points for the species that appeared, two large groups of similarities were classified (similarity group 1: 2003 sites, similarity group 2: 2018 sites).

Studies on Acid-stable Alpha-amylase (내산성 ${\alpha}-Amylase$에 관한 연구)

  • Kim, Hack-Joo;Byun, Si-Myung
    • Applied Biological Chemistry
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    • v.21 no.2
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    • pp.103-108
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    • 1978
  • Acid-stable ${\alpha}-amylase$ was partially purified from Paecilomyces subglobosum by Sephadex G-150 gel filtration. About 7.7-fold purification was obtained and the partially purified preparation has 5.0 U of ${\alpha}-amylase$ activity per mg of protein. Using this partially purified ${\alpha}-amylase$, general properties were studied and it showed the maximal activities at the conditions of pH 4.0 and $38^{\circ}C$. High stability of the acid-stable ${\alpha}-amylase$ in acidic condition was observed, whereas thermal stability was similar to the conventional ${\alpha}-amylase$.

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