• Applied Biological Chemistry
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• 제14권3호
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• pp.201-205
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• 1971

Moving boundary, paper 및 starch gel electrophoresis와 column chromatography에 의한 난단백의 분리에 대하여서는 오랫동안 연구되어 왔으나 본 실험에서는 난단백의 thick층과 thin층의 부화과정의 변화에 대하여 acrylamide gel을 이용한 disc electrophoresis를 통하여 필름으로 기록 관찰한 결과를 보고한다.

• 동의생리병리학회지
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• 제19권5호
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• pp.1162-1168
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• 2005

For raising to the significance of whole Dody form diagnosis, I studied comparatively oriental medicine's whole body form diagnosis, biological evolution and physiognomy. In the oriental medicine, the whole body form diagnosis was recognized a means to deal with 'an individual physiology-pathology' and applied practically 'the typing of whole body form'. The physiognomy was applied to whole body form for the conversion of conception in social science and attached great importance to 'the face of human'. The biological evolution was applied to whole body form for the means by grasping the human individual characteristic, so much as magnified to the size of unconscious body-language(Synergologie, Tells exe.).

• 한국균학회지
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• 제42권3호
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• pp.183-190
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• 2014

사과 갈색무늬병은 최근 우리나라 사과 과수원에 심각한 경제적 피해를 입히는 질병이다. 본 총설에서는 우리나라에서의 사과 갈색무늬병 연구역사와 갈색무늬병의 발생생태, 병원균의 생활환, 그들의 형태학적, 배양학적, 유전학적 특징 및 분리배양 기법에 대해 종합적으로 기술하였다. 또한, 후지 품종에 빈번히 발생하는 원인미상의 갈색무늬병 유사증상에 대한 특징도 상술하였다.

• Journal of Applied Biological Chemistry
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• 제42권3호
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• pp.151-153
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• 1999

Gromwell (Lithospermum erythrorhizon) pigment solution and Jindo Hongju prepared in the laboratory showed characteristic pH-dependent color changes and a shift in absorption maxima. This phenomenon was not observed in the solution of the artificial food colorant Red No. 2 which was suspected to be used in the manufacture of fake Jindo Hongju. A few fake products could be detected by using the pH-dependent shift in absorption maxima among the Jindo Hongju on market.

• 대한불안의학회지
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• 제13권1호
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• pp.1-9
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• 2017

We reviewed the scientific literature on psychological effects of Chemical, Biological, Radiological, Nuclear, Explosive (CBRNE) disaster, which is one of the biggest threat to modern society. CBRNE disaster has the potential to cause specific physical symptoms and psychological distress in victims ; moreover, various toxic symptoms and carcinogenesis/mutation would be an important issue. Bioterrorism can cause localized outbreaks of infectious disease or pandemic disaster. Somatization as well as posttraumatic stress symptoms and depression are the characteristic psychological symptoms in CBRNE disaster's victims. CBRNE disasters could lead to large-scale public fear and social chaos due to the difficulties involved in verifying the extent of exposure and unfamiliar area to the common people. In the evacuation process, problems associated with adjustment and conflict between victims and residents should be considered.

• 대한의용생체공학회:의공학회지
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• 제28권6호
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• pp.768-776
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• 2007

A new type of the fluid circulation blood pressure simulator was proposed to enhance the blood pressure simulator used for the development and evaluation of automatic sphygmomanometers. Various pressure waveform of fluid flowing in the pipe was reproduced by operating the proportional control valve after applying a pressure on the fluid in pressurized oil tank. After that, appropriate fluid was supplied by operating the proportional control valve, which enabled to reproduce various pressure wave of the fluid flowing in the tube. To accomplish this work, the mathematical model was carefully reviewed in cooperating with the proposed simulator. After modeling the driving signal as input signal and the pressure in internal tube as output signal, the simulation on system parameters such as internal volume, cross-section of orifice and supply pressure, which are sensitive to dynamic characteristic of system, was accomplished. System parameters affecting the dynamic characteristic were analyzed in the frequency bandwidth and also reflected to the design of the plant. The performance evaluator of fluid dynamic characteristic using proportional control signal was fabricated on the basis of obtained simulation result. An experimental apparatus was set-up and measurements on the dynamic characteristic, nonlinearity, and rising and falling response was carried out to verify the characteristic of the fluid dynamic model. Controller was designed and thereafter, simulation was performed to control the output signal with respect to the reference input in the fluid dynamic model using the proposed proportional control valve. Hybrid controller combined with an proportional controller and feed-forward controller was fabricated after applying a disturbance observer to the control plant. Comparison of the simulations between the conventional proportional controller and the proposed hybrid simulator indicated that even though the former showed good control performance.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.459-462
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• 2001

Angiopoietin-2 (Ang2) is a naturally occurring antagonist for angiopoietin-l (Angl) and its Tie2 receptor during vasculogenesis, Although angiopoietins have been expressed in several mammalian cell lines, their expression levels are low. Recombinant Chinese hamster ovary (CHO) cell lines expressing a high level of human Ang2 or its analog, human $Ang2_{443}$, with an amino-terminal FLAG-tag were constructed by transfecting the expression vectors into dhfr-deficient CHO cells and subsequent gene amplification in medium containing stepwise increments in methotrexate level. Secreted Ang2 or human $Ang2_{443}$ were purified from the cultured medium using an anti-FLAG- agarose affinity chromatography, The purified Ang2 and $Ang2_{443}$ migrated on SOS-PAGE as a broad band, characteristic of glycosylated protein. Their biological activity in vitro was demonstrated in a serum deprivation-induced apoptosis assay. Ang2 at high concentration, like AngI, can be an apoptosis survival factor for endothelial cells through the activation of the Tie2 receptor.

• 한국자원식물학회지
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• 제33권5호
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• pp.476-481
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• 2020

한반도 미기록식물인 얼치기대가래(Potamogeton × anguillanus Koidz.)를 강원도 영월군 남면 연당리 연당천에서 발견하였다. 얼치기대가래는 대가래(P. wrightii Morong)와 넓은잎말(P. perfoliatus L.)의 자연교잡종이다. 대가래에 비해 잎자루가 짧고, 넓은잎말에 비해 잎이 좁고 길며, 밑부분이 줄기를 완전히 감싸지 않는 특징으로 구별된다. 본 미기록식물의 국명은 교잡종임을 고려하여 얼치기대가래로 신칭하였다. 주요 형질에 대한 기재와 화상자료 및 검색표를 제시하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제28권1호
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• pp.31-41
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• 2002

Proline-rich proteins (PRPs) are major components of human saliva. In order to know the biological roles of PRPs, we explored the expression pattern and functional protein structures of PRPs by the immunohistochemical and various molecular biological methods. Polyclonal antibody against human gPRP was generated from rabbit by the injection of oral exfoliated cells specially treated by urea and SDS buffer. The PRPs began to be expressed both in the acinar cells and ductal cells from the EIDS (Early Intermediate Developmental Stage) of fetal salivary glands and became intense in the salivary epithelium in the LDS (Late Developmental Stage) and adult salivary glands. The polyclonal antibody against the gPRP showed the cross-reactivity with aPRP and bPRP, these results were relevant to the high homology among subtypes of PRP. However, the simulated protein structures of PRPs showed the characteristic repetitive whorling domains except the N-terminal signal peptide. The whorling domains were also contained the multiple amino acids of glutamine and glycine, which may provide the receptor binding or cross-linking sites of PRPs.

• Journal of Preventive Medicine and Public Health
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• 제42권6호
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• pp.349-355
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• 2009

Biomarkers are characteristic biological properties that can be detected and measured in a variety of biological matrices in the human body, including the blood and tissue, to give an indication of whether there is a threat of disease, if a disease already exists, or how such a disease may develop in an individual case. Along the continuum from exposure to clinical disease and progression, exposure, internal dose, biologically effective dose, early biological effect, altered structure and/or function, clinical disease, and disease progression can potentially be observed and quantified using biomarkers. While the traditional discovery of biomarkers has been a slow process, the advent of molecular and genomic medicine has resulted in explosive growth in the discovery of new biomarkers. In this review, issues in evaluating biomarkers will be discussed and the biomarkers of environmental exposure, early biologic effect, and susceptibility identified and validated in epidemiological studies will be summarized. The spectrum of genomic approaches currently used to identify and apply biomarkers and strategies to validate genomic biomarkers will also be discussed.