• Microbiology and Biotechnology Letters
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• v.23 no.2
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• pp.119-122
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• 1995

A different form from Gibberella fujikuroi was isolated from the paddy field of Naju area. The strain, designated as Y107, was identified as Gibberrella sp. based on its morphological, physiological, and biochemical characteristics. The highest production of Gibberellic acid by the strain was achieved in a fermentation medium containing corn starch, glucose, soybean oil, soybean meal, NH$_{4}$NO$_{3}$, K$_{2}$HPO$_{4}$, MgSO$_{4}$, and trace elements.

• Korean Journal of Veterinary Research
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• v.31 no.3
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• pp.271-277
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• 1991

To investigate the epidemiological trait of listeriosis, Listeria spp were isolated from poultry meat, products and environmental specimens in chicken slaughterhouse. Also determined were isolation rates by the different enrichment procedures, the biochemical properties of isolates. In a total of 307 samples including poultry meat, liver, feathers, feces, chiller water, scalding water overflow and slaughterhouse floor, Listeria spp were isolated predominantly from scalding water overflow (90.0%), body skin before washing (66.7%), liver (20.0%) and feathers(15.0%) However, few Listeria spp were isolated from body skin after washing and feces. The higher isolation rates were obtained in the secondary enrichment procedure (7.2%) than in the primary enrichment (3.9%); after stored the secondary enrichment cultures for 2 weeks at $4^{\circ}C$, Listeria spp were present in 9.8%. The majority of the isolated Listeria spp were identical to those of the standards strains in biochemical and cultural properties. Overall, Listeria spp were present in 13.4% of the specimens tested, and were in order of prevalence of L innocua(11.1%), L monocytogenes(3.3%), L grayi(0.7%) and L murrayi(0.3%).

• Korean Journal of Veterinary Service
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• v.13 no.1
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• pp.69-74
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• 1990

The present study was conducted to investigate the incidence of pasteurella multocida infection in kyungbuk swine herds during the period from July 1989 to November 1989 and some properties of the isolated organisms. P. multocida was isolated from lungs of 155 slaughtered pigs, 43(27.7%) pigs were culture positive. The majority of biochemical and cultural properties of the P. multocida isolates were identical to those of the standard strains. The capsular serogroups and drug susceptibility of 43 isolates of P. multocida from pigs with pneumonic lesions were investigated. P. multocida isolates were typed for capsular serogroupes A by hyaluronidase inhibition of capsule and D by acriflavin auto agglutination. Most isolates(60.4%) were type A, 18.6% were type D, and the remaining 21.0% were untypable. In antimicrobial susceptibility test these isolates of P. multocida were susceptible in order of ampicillin (86.0%), trimethoprim sulfamethoxazole(83.7%), colistin(81.4%), chloramphenicol(79.1%), but the majority of them were resistant in order of streptomycin(30.2%), triple sulfa (4.6%).

• Korean Journal of Veterinary Research
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• v.35 no.2
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• pp.297-306
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• 1995

The present study was conducted to investigate the incidence of Streptococcus suis infection in the pneumonic lungs of slaughter pigs from December 1992 to April 1994 and to examine the biochemical and cultural properties, the antimicrobial susceptibility and serotype of the isolates. Fifty-six(7.95%) strains of S suis was isolated from 704 pneumonic lungs of slaughter pigs, of which 33 cultures were isolated alone, while remaining 18 were complicated wide Pasteurella multocida. Of the 56 S suis isolates, 18(32.1%) were serotype 2, 5(8.9%) were serotype 1/2, 2 were serotype 1, 1 each for serotype 3 & 5, and the remaining 29 were untypable. The twenty five strains(44.6%) of all isolates produced hyaluronidase while 15 strains(88.3%) of 18 serotype 2 isolates were hyaluronidase producers. All of isolates were highly susceptible to ampicillin, amoxicillin, ceftiofur, cephalothin, ciprofloxacin and penicillin G, although the majority of them were resistant to kanamycin, colistin, erythromycin, oxytetracycline.

• Korean Journal of Veterinary Service
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• v.14 no.1
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• pp.49-61
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• 1991

The present study was conducted to investigate biochemical, serologic, and pathogenic characteristic of E. rhusiopathiae isolated from the cases of acute septicemic swine erysipelas in Youngnam provinces during the period from June 1988 to September 1990. The majority of biochemical and cultural properties of E. rhusiopathiae isolated from pigs affected with acute erysipelas were identical to those of the standard strain employed. All of the 45 isolates were serotype la. All isolates were highly susceptible to penicillin G, lincomycin, cephalothin, ampicillin, erythromycin (MIC : 0.025-0.78IU or ${\mu}g$ / ml ), and moderately susceptible to oleandomycin, oxytetracycline, chloramphenicol (MIC : 0.78-25${\mu}g$ / ml ). Kanamycin and sulfadimethoxine showed no activity against the isolates(MIC : ＞400${\mu}g$ / ml ). The MICs of dihydrostreptomycin presented two distribution peaks ; of 45 strains, 5(11.1%) were resistant to dihydrostreptomycin(MIC : 400${\mu}g$ / ml ). All of 5 selected isolates were pathogenic for mite and $LD_{50}$ was $3.7{\times}10^3$viable cells. Mice immunized subcutaneously with live vaccine did not die after challenge to virulent isolates of E. rhusiopathiae.

• Korean Journal of Microbiology
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• v.9 no.1
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• pp.1-26
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• 1971

Intensive taxonomical studies of the Aspergilli have long been made. Altogether 132 species and 18 varieties are recognized in the book "The Genus Aspergillus" written by Raper and Fennell (1965), in contrast to 77 species, 8 varieties and 4 mutations in " A Manual of Aspergilli" written 20 years earlier by Thom and Raper (1945). Classification of the Asperilli by Thom and Raper (1945) and by Raper and Fenell (1965). Classification of the Aspergilli by Thom and Raper (1945) and by Rapher and Fenell (1965) have been based mainly upon morphological and cultural detail both physiological and biochemical activities. In Korean there are many kinds of foods fermented natrually without the employment of selected microorganisms, and there are, of course, many different microorganisms serving in the fermentation fermented foods than other countries, the distribution and biological properties of the Asperigilli, in Korea are more variable. Taxonominical studies with 36 strains of Asperilli were based upon the examinations of morphological, cultural, and physiological characteristics. nineteen strains indigenous to Korea were selected from a lot of strains which had been isolated from meju and kokja and one strain from soil. They were identified according to the group key of Raper and Fennell. Ten strains were donated by Dr.Hesseltine of the Northern Utilization Research and Development Division in the U.S.A. From the Asp. japonicus supplied by Dr.Hesseltine, a white mutant was isolated and also studied. Two strains were donated by Dr. Murakami of the Research Institute of Brewing in Japan, and four strains came from Korean industrial companies.ndustrial companies.

• Journal of Food Hygiene and Safety
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• v.15 no.3
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• pp.271-276
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• 2000

A amylase producing bacteria were isolated from tofu residue and identified as Bacillus sp. according to the morphological and biochemical properties, which were named Bacillus sp. GM7330 and Bacillus sp. GM7312. The cultural condition for the production of amylase was showed on 5% tofu residue added 3% glucose and 0.15% yeast extract. And incubated during 72 hrs at 30。C, Bacillus sp. GM7330 and Bacillus sp. GM7312 were producing amylase of 488 units and 341 units.

• Korean Journal of Microbiology
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• v.22 no.4
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• pp.257-263
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• 1984

A strain producing an extracellular cytosine deaminase was isolated from soil samples. The enzyme obtained from the strain possessed the substrate specificity to both cytosine and 5-fluorocytosine. From the results of its morphological, cultural, physiological, and biochemical properties, the strain was thought to be the genus Arthrobacter. Therefore, it was named as Arthrobacter sp. JH-13. The composition of optimum medium for the enzyme formation was 0.5% of peptone, 0.5% of meat extract, 0.5% of soluble starch, and 0.1% of KCl. The optimum pH for the enzyme formation was 8.0. When the microoganism was cultured aerobically in the above medium, enzyme production reached at maximum in 54 hours at $30^{\circ}C$.

• Korean journal of food and cookery science
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• v.24 no.1
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• pp.11-15
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• 2008

In searching for yeast to be utilized as biocontrol agents, a single yeast strain was isolated from Camellia sinensis based on its morphological, cultural, physiological, and biochemical properties, as well as by molecular techniques. This single strain was pink to red in color and designated as strain JY-1. The effects of temperature, pH, NaCl concentration, and ethanol concentration on the growth of the JY-l strain were examined for the JY-1. Growth occurred at temperatures ranging from 20 to $35^{\circ}C$, and between pH 3.0 and 12.0, with optimal growth at $25-30^{\circ}C$ and pH 5.0. The yeast also grew in the presence of 0-2% (w/v) NaCl and 0-4% (v/v) EtOH. The isolate was further classified based on biochemical characteristics using the VITEK system. The biochemical data obtained using this system were similar to those of Rhodotorula glutinis/Rhodotorula mucilaginosa (exhibiting a 93% matching level). Molecular phylogenetic analysis based on l8S rDNA sequences indicated that the yeast represented a basidiomycetous species, and its highest degree of sequence similarity was with Rhodosporidium azoricum, strain JCM11251 (99%).

• Korean Journal of Veterinary Research
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• v.36 no.1
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• pp.181-186
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• 1996

The present study was conducted to investigate the biochemical and serologic characteristic of Actinobacillus pleuropneumoniae isolated from pneumonic lungs of pigs during the period from January 1992 to April 1993. A pleuropneumoniae was isolated from 17(27.0%) of 63 growing pigs with respiratory signs and 21(6.4%) of 330 pneumonic lungs of slaughtered pigs. The seasonal isolation frequency of A pleuropneumoniae was higher in winter and spring than that in summer or fall. The biochemical and cultural properties of A pleluropneumoniae isolated from the pneumonic lungs of pigs were identical to those of the reference strains used. The isolates were highly susceptible to ampicillin, cephalothin, ceftiofur, ciprofloxacin(MIC : ${\leq}0.39{\mu}g/ml$) and moderately susceptible to amikacin, chloramphenicol, erythromycin, kanamycin, methicillin, penicillin-G, streptomycin(MIC : 0.78~25IU or ${\mu}g/ml$), respectively. Sulfadimethoxine, sulfamerazine, tylosine showed no response to the isolates(MIC : ${\geq}100{\mu}g/ml$). Among the 38 isolates, 21(55.3%) and 13(34.2%) were resistant to oxytetracycline aid lincomydn, respectively(MIC : ${\geq}50IU$ or ${\mu}g/ml$). The majority of 38 A pleuropneumoniae isolates were turned out as serotype 2(47.4%) or serotype 5(54.7%) and the remaining 3 isolates were evenly classified to serotype 7, 10 or 12. It was noted A pleuropneumonine serotype 5 isolates were more resistant to oxytetracycline than serotype 2 isolates.