• Journal of Animal Science and Technology
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• 제59권10호
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• pp.22.1-22.7
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• 2017

Background: Very limited information exists on the ruminal degradation kinetics of nutrients in garlic stalk. The present study aimed to survey the annual yield of garlic stalk in Korea and determine its feed-nutritive value for ruminants. Methods: In Experiment 1, garlic stalk was incubated in situ in the rumen of two Hanwoo steers ($360{\pm}15kg$ body weight) and removed after 12, 24, or 48 h to determine the ruminal degradation kinetics of DM and NDF. Rice straw was also included for comparison. In Experiment 2, In Experiment 2, six male Corriedale sheep were randomized to two dietary treatments to determine the apparent digestibility of nutrients in garlic stalk. Diets included a control ration without garlic stalk (60% concentrate mix +40% ryegrass) or a treatment ration (70% control diet +30% garlic stalk). Results: The Korean national yield of garlic stalk (sun-dried basis) in 2016 was estimated to be 31,910 tons, with the southern coastal regions producing the highest quantity. Compared with rice straw, garlic stalk had lower NDF, higher ADF, and greater effective degradabilities of DM and NDF, resulting in a greater TDN value (56.3%), which was higher than that obtained for rice straw (43.7%). Conclusion: These results provide basic information on the ruminal DM and NDF degradation kinetics of garlic stalk, which would be helpful for the efficient utilization of this by-product in ruminant diets

• Asian-Australasian Journal of Animal Sciences
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• 제33권3호
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• pp.436-445
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• 2020

Objective: Ensiling is a simple and effective method for long-term preservation; however, less information exists about the ensilability characteristics of garlic stalk (GS). Therefore, the objectives were to examine the ensiling feasibility of GS. Methods: The GS was ensiled alone or inoculated with Lactobacillus plantarum KU5 in the presence or absence of 5% molasses and ensiled for 7, 14, and 28 d. As an alternative storage method, GS was co-ensiled with wet citrus pulp (CP) at different proportions (GS:CP: 70:30, 60:40, 50:50, and 40:60). Analysis was made on physicochemical, fermentative, and nutritional parameters. Results: The GS was found to be a biomass which is difficult to ensile. A combination of microbial inoculant and molasses was successful in the improvement of the silage fermentation quality of GS. Co-ensiling of GS with wet CP at the mixing ratio of 50:50 provided the most desirable silage fermentation parameters, including the substantial lactic acid formation, low final pH, minor effluent loss, and the more favorable organoleptic properties. Conclusion: Co-ensiling GS with CP appears to be a simple and viable method of conservation, enabling the more efficient utilization of these by-product resources over a prolonged period.

• 센서학회지
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• 제27권1호
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• pp.55-58
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• 2018

Low-cost and large-scale fabrication method of nanohole array, which comprises nanoscale voids separated by a few tens to a few hundreds of nanometers, has opened up new possibilities in biomolecular sensing as well as novel frontier optical devices. One of the key aspects of the nanohole array research is how to control the hole size following each specific needs of the hole structure. Here, we report the extensive study on the fine control of the hole size within the range of 500-2500 nm via surface-catalyzed chemical deposition. The initial hole structures were prepared via conventional photo-lithography, and the hole size was decreased to a designed value through the surface-catalyzed chemical reduction of the gold ion on the predefined hole surfaces, by simple dipping of the hole array device into the aqueous solution of gold chloride and hydroxylamine. The final hole size was controlled by adjusting reaction time, and the optimal experimental condition was obtained by doing a series of characterization experiments. The characterization of size-controlled hole array was systematically examined on the image results of optical microscopy, field emission scanning electron microscopy(FESEM), atomic-force microscopy(AFM), and total internal reflection microscopy.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.603-609
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• 2002

DFA IV is di-D-fructose-2,6':6,2'-dianhydride, consisting of two fructose residues. It can be enzymatically synthesized from levan by levan fructotransferase, and can be used for mineral absorption. Understanding of the structure and composition of levan is important to obtain high-level production of DFA IV. A bacterial strain, Pseudomonas aurantiaca 5-4380, was identified to produce low-branched levan, and the levansucrase gene (lsch) from this bacterium was found to be composed of 1,275 Up coding for a protein of 424 amino acids, with an estimated molecular weight of 47 kDa. The bacterial levansucrase gene was expressed in Escherichia coli DH5${\alpha}$ by its own promoter and lac promoter. The recombinant levansucrase was produced in soluble form with 170U of levansucrase activity from 1-ml E. coii culture broth. The expressed enzyme from the clone showed similar biochemical properties, such as size of active levansucrase, degree of branching, and optimum temperature, with P.aurantiaca 5-4380 levansucrase.

• 대한방사선기술학회지:방사선기술과학
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• 제37권3호
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• pp.169-175
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• 2014

2013년 국민건강보험심사평가원의 의료기관 데이터베이스에 따르면 1118개의 병.의원에 영상의학과가 개설되어있다. 이들 병원에는 CT, 투시촬영장치, 일반촬영장치와 같은 의료용 방사선 발생장치가 운영되고 있다. 이 중에서도 일반촬영장치는 가장 많은 병원에서 운영되고 있는 장비이다. 일반촬영장치의 경우 film-screen 장치에서 digital radiography 로 급격하게 변하고 있다. 하지만 그 촬영기법은 films-screen 기법을 그대로 사용하고 있어 디지털 장치의 맞는 촬영기법의 개발을 위한 전반적인 실태 조사가 필요하다. 이에 본 연구에서는 국내 의료기관의 일반촬영기법에 관한 조사를 시행하여 실제 병원에서 사용하고 있는 일반촬영기법의 기술적 항목에 대한 현황을 파악하여 보았다. 본 연구에서는 의료기관에서 일반적으로 사용되는 일반촬영기법 26개에 대한 촬영기법의 전국 단위 조사에서 흉부, 두부, 척추, 골반에 해당하는 검사에 대한 분석을 시행하였다.

• 대한수의사회지
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• 제48권12호
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• pp.725-729
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• 2012

• 대한의용생체공학회:의공학회지
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• 제26권5호
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• pp.271-282
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• 2005

There are strong demands for accurate, fast, and inexpensive devices in the medical diagnostic laboratories, such as biosensors and chemical sensors. Biosensors can provide the reliable and accurate informations on the desired biochemical parameters, which is an essential prerequisite for a patient before going for a treatment. They can be used for continuous measurements of metabolites, blood cations, gases, etc. Of these, electrochemical biosensors play an important role in the improvement of public health, because rapid detection, high sensitivity, small size, and specificity are achievable for clinical diagnostics. In this paper, the clinical applications with electrochemical biosensors are reviewed. An attempt is also made to highlight some of the trends that govern the research and developments of the important biosensors that are associated to clinical diagnosis.

• Journal of Microbiology and Biotechnology
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• 제12권6호
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• pp.1022-1025
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• 2002

Club root is one of the major diseases that occur in crucifers. It is caused by Plasmodiophora brassicae. In order to discover microbial biopesticides against P. brassicae, forty-eight Streptomyces isolated from soil were screened. Among these, three strains showed excellent pesticidal activities. We report results on in vivo screening with fermentation broths of these strains and identification of the strain taxa.

• KIEAE Journal
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• 제14권6호
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• pp.23-29
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• 2014

It is essential to investigate the structure and the main characteristics of BSN (Bio-Sensor Network) platform in built smart healthcare environment while designing healthy housing facilities. For this study, WSN (Wireless Sensor Network) data transmission technologies have been employed with medical sensors, and optimal medical devices would provide various Web 2.0 services by connecting to the WiBro network. The BSN platform normally recognizes in surroundings of WBAN (Wireless Body Area Network) or WPAN (Wireless Personal Area Network), and it is possible to manage sensor nodes by utilizing SOAP (Simple Object Access Protocol) and REST (REpresentational State Transfer). In addition, the feature of SNMP (Simple Network Management Protocol) for mobile gateway is also included for being adapted to huge network structure. Finally, BSN platform will play a role as important clues for developing personal WSN service models for smart healthy housing properties.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 학술대회
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• pp.14-17
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• 2008

Recently, several advanced flow visualization techniques such as Particle Image Velocimetry (PIV) including stereo PIV, holographic PIV, and dynamic PIV have been developed. These advanced techniques have strong potential as the experimental technology which can be used for verifying numerical simulation. In addition, there would be indispensable in solving complicated thermo-fluid flow problems not only in the industrial fields such as automotive, space, electronics, aero- and hydro-dynamics, steel, and information engineering, but also in the basic research fields of medical science, bio-medical engineering, environmental and energy engineering etc. Especially, NT Nano Technology) and BT (Bio Technology) strongly demand these advanced measurement techniques, because it is difficult for conventional methods to observe most complicated nano- and bio-fluidic phenomena. In this paper, the basic principle of these advanced visualization techniques and their practical applications which cannot be resolved by conventional methods, such as flow in automotive HVAC system, ship and propeller wake, three-dimensional flow measurement in micro-conduits, and flow around a circulating cylinder will be introduced.