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Effect of ${\beta}$-Glucosidase as a Feed Supplementary on the Growth Performance, Digestive Enzymes and Physiology of Broilers

  • Qian, L.C.;Sun, J.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.2
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    • pp.260-266
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    • 2009
  • The effects of ${\beta}$-glucosidase on the overall growth performance and a set of physiological parameters of broilers were investigated. 240 male, one-day old Avine broiler chickswere randomly allocated to four treatment groups and fed with a corn-soybean meal supplemented with 0% (control), 0.2%, 0.4% and 0.6% ${\beta}$-glucosidase. The 0.2% ${\beta}$-glucosidase group, but not the 0.4% and 0.6% ${\beta}$-glucosidase groups, showed a significantly increased average daily weight gain (p<0.05) over that of the control. All three ${\beta}$-glucosidase feed groups showed significantly higher feed conversion ratios than the control group (p<0.05). Feed supplementation of 0.2% ${\beta}$-glucosidase significantly raised the contents of serum isoflavone aglycones as shown by decreases of genistin and daizin (p<0.01) and an increase of daidzein (p<0.01). The 0.2% ${\beta}$-glucosidase feeding significantly increased the intestinal amylase activity while it had little effect on lipase and trypsin activities (p>0.05). 0.2% ${\beta}$-glucosidase feeding also significant elevated the levels of highdensity lipoprotein cholesterol and malate dehydrogenase while lowering the level of low-density lipoprotein cholesterol (LDL-C). Finally, ${\beta}$-glucosidase improved the anti-oxidative activities of the animals; the 0.2% ${\beta}$-glucosidase feed group had higher activities of superoxide dismutase (p<0.05), glutathione peroxidase and glutathione reductase in the liver (p<0.05), and malondialdehyde level in the serum (p<0.05).

Prediction of Quality of Life among the Elderly at Care Facilities for the Elderly according to Health States, Physical and Cognitive Functions, and Social Supports-Focused on D Metropolitan City (노인요양시설 노인의 건강상태, 신체적, 정신적 기능, 사회적 지지에 따른 삶의 질 예측요인-D 광역시를 중심으로-)

  • Kim, Jong-Im
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.7
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    • pp.4656-4667
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    • 2015
  • The purpose of this study was to investigate relations among the quality of life, health states, physical functions, cognitive functions, and social supports of the elderly at care facilities. The subjects include the elderly that were living in care facilities in some urban areas and aged 65 or older. The data of total 260 old people were used in analysis. Collected data were analyzed with descriptive statistics, t-test, ANOVA, Pearson correlation, and stepwise multiple regression. The elderly at care facilities, who were considered as physically and cognitive vulnerable, scored higher means on quality of life when they were satisfied with the length of stay and sleeping state, had higher subjective health states, had no tooth inconvenience and forgetfulness, suffered from a lower level of depression, had better cognitive functions, and received higher social supports. Quality of life had correlations with the sleeping state(r=-.20, p<.001), subjective health state(r=-.24, p<.001)s, depression(r=-.30, p<.001), and social supports(r=.30, p<.001). Social supports(${\beta}=.30$, p<.001), depression(${\beta}=-.25$, p<.001), subjective health states(${\beta}=-.22$, p<.001), length of stay(${\beta}=-.22$, p<.001), and sleeping state(${\beta}=-.12$, p=.025) turned out to predict the quality of life of the elderly at care facilities and have close relations with it. In short, the quality of life of the elderly is related to many different factors at care facilities. The findings indicate that nursing interventions and managements for quality of life require a mental and social approach or a whole person approach with a focus on the understanding of individual senior citizens rather than on physical activities and diseases.

Effects of Relaxin and Insulin on Porcine Granulosa-lutein Cell Steroidogenesis In Vitro (체외에서 돼지 황체화 과립막세포의 스테로이드 호르몬 생산에 미치는 Relaxin과 Insulin의 영향)

  • Lee M. S.;Hossein M. S.;Lee C. K.;Kang S. K.;Lee B. C.;Hwang W. S.
    • Journal of Embryo Transfer
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    • v.20 no.1
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    • pp.71-77
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    • 2005
  • To investigate the influence of relaxin and insulin on the ovarian steroid secretion of porcine granulosa cells, we used porcine granulosa cells partially luteinized in a primary culture and examined the production of progesterone and $17{\beta}-estradiol$. Porcine granulosa cells were cultured in the presence of serum for 48 h after attachment and subsequently in the absence of serum fur 24 h. To confirm the dose dependency of relaxin or insulin, various concentrations (10, 100, 1000 ng/ml) of relaxin or insulin were added in the medium for the last 24 h, respectively. To investigate the combinational effect of relaxin and insulin, 100 ng/ml relaxin and/or 100 ng/ml insulin were added in the medium for the last 24 h in the presence or absence of luteinizing hormone (100 ng/ml). The medium was collected and used for radioimmunoassay to measure the production of progesterone and $17{\beta}-estradiol$. Relaxin or insulin increased the production of progesterone by dose dependency, respectively while they had no effect of the production of $17{\beta}-estradiol$. Relaxin (100 ng/ml) and/or insulin (100 ng/ml) significantly increased the production of progesterone in the presence of luteinizing hormone while they had no effect of the production of $17{\beta}-estradiol$. In conclusion, relaxin and/or insulin increased the progesterone secretion of porcine granulosa-lutein cells in vitro while had no effect on the production of $17{\beta}-estradiol$ and had no synergism on the effects. The effects of relaxin and/or insulin on the production of progesterone were augmented by the presence of luteinizing hormone.

Genotypic Variations in ${\beta}-glucan$ Content of Barley Cultivated in Different Regions

  • Kim Hong-Sik;Park Kwang-Geun;Baek Seong-Bum;Nam Jung-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.4
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    • pp.335-339
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    • 2006
  • The level of ${\beta}-glucan$ which is a major soluble dietary fiber found in the grain endosperm cell wall was highly variable among 25 barley genotypes grown at four locations including Suwon, Naju, Jinju, and Jeju. Statistically significant genotypic effects were observed for ${\beta}-glucan$ content at each or across growing sites (P<0.001). On average, 'Chalssalbori' showed the lowest percentage ${\beta}-glucan$ (4.04%) among genotypes in the grain, whereas 'Yonezawa Mochi' was highest in percentage ${\beta}-glucan$ (6.46%) compared to other genotypes. The significant difference between genotypes was approximately 1-2% across environments. The effects of location or interaction between locations and genotypes were not significant on the variation of ${\beta}-glucan$ contents. High ${\beta}-glucan$ content seemed to be greatly associated with such grain traits as waxiness and presence of husk except for 'Chalssalbori'. The waxy genotypes had a mean of 5.37% and values ranging from 5.28 to 5.47%, but normal genotypes had a mean of 4.78% and values ranging from 4.69 to 4.88% over environments. Hulless barley genotypes were also higher than hulled barley genotypes for the average ${\beta}-glucan$ content in both individual and over all environments. The difference between the hulled and hulless gene pools was on average of 0.37% with ranges from 0.19% to 0.56% at four environments. ${\beta}-glucan$ content measured from a mapping population of $F_5$-derived 107 lines derived from the cross between 'Yonezawa Mochi' and 'Neulssalbori' was not significantly associated with other agronomic traits except for 1,000-kernel weight at the '01 Suwon environment. Not too much information on the relationship of ${\beta}-glucan$ content to agronomic traits was available.

Tocopherol and Carotenoid Contents of Selected Korean Cooked Combination Foods Consumed by Young Korean Children

  • Kim, Young-Nam;Giraud David W.;Driskell Judy A.
    • Nutritional Sciences
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    • v.9 no.4
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    • pp.323-329
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    • 2006
  • To more accurately estimate vitamin A and vitamin E intake of Koreans, food composition databases of the tocopherol and carotenoid contents of Korean foods are needed. In this study, the tocopherol $(\alpha-,\;\gamma-,\;and\;\delta-)$ and carotenoid ($\alpha-carotene,\;\beta-carotene,\;\beta-ctyptoxanthin$, lutein, and zeaxanthin) contents of 12 Korean cooked combination foods commonly consumed by children in Kwangju, Republic of Korea, were determined using reversed-phase HPLC. All samples were obtained from 3 different households in Kwangju during summer, 2005. All cooked foods in this study had detectable quantities of $\alpha-tocopherol,\;\gamma-tocopherol$ except for shoegogimugook, and $\delta-tocopherol$ except for myulchibokkeum Doejigogibokkeum had the highest $\alpha-tocopherol$ content (0.64 mg/l00 g edible portion), and $\gamma-tocopherol\;and\;\delta-tocopherol$ contents of gimbab were the highest among the foods (1.01 and 0.26 mg/l00 g edible portion). $\beta-carotene$ was found in all food samples. Gimbab had the highest contents of $\alpha-carotene,\;\beta-carotene$, and lutein among Korean cooked combination foods (158.3, 266.6, and $375.4{\mu}g/100g$ edible portion). Miyukgook contained only $\beta-carotene$ ($2.5{\mu}g/100g$ edible portion). Considerable sample-to-sample variability in tocopherol and carotenoid compositions were observed in several Korean cooked combination foods included in this study. Some of these carotenoids $(\alpha-carotene,\;\beta-carotene,\;and\;\beta-cryptoxanthin)$ are vitamin A precursors. The findings of this study may be valuable for use in Korean databases as well as nutrient consumption research for vitamin A and vitamin E.

Regulation of $\beta$-galactosidase Biosynt hesis in Lactobacillus sporogenes (Lactobacillus sporogenes에서$\beta$-galactosidase 생합성 조절)

  • 이정희;최용진
    • Microbiology and Biotechnology Letters
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    • v.18 no.6
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    • pp.566-570
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    • 1990
  • Regulation of $\beta$ -galactosidase formation was studied with Lactobacillus sporogenes. Synthesis of the enzyme was effectively induced by isopropyl- $\beta$-D-thiogalactopyranoside (IPTG) or galactose, and to a much lower level by lactose. When 15 mM glucose was added at the different intervals to the cultures that had been in contact with IPTG, the same levels of inhibition of the enzyme synthesis were observed (approximately one-third the differential rate of a control culture without glucose). This suggests that glucose did not interfere with the entry of the inducer into the cells, but interfere with the formation of $\beta$ -galactosidase through catabolite repression. The glucose inhibitory effect was not overcome by adding CAMP or cGMP to the culture media.

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Synergistic effects of dietary $\beta$-1,3 glucan and feed stimulants in juvenile olive flounder (Paralichthys olivaceus)

  • Sungchul C. Bai;Park, Gunjun;Kim, Joon-Hyung;Cui Hua;Kim, Youngchul
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.68-69
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    • 2003
  • The present study was conducted to investigate the effects of dietary supplementation of $\beta$-1,3 glucan and feed stimulants(BAISM) as a feed additive for juvenile olive flounder (Paralichthys olivaceus). Eight experimental diets supplemented with $\beta$-1,3 glucan and feed stimulants at 0%, $\beta$-1,3 glucan 0.05% + Baism 0.45%, $\beta$-1,3 glucan 0.05% + Baism 0.95%, $\beta$-1,3 glucan 0.10% + Baism 0.90%, $\beta$-1,3 glucan 0.10% + Baism 1.90%, $\beta$-1,3 glucan 0.15% + Baism 1.35%, $\beta$-1,3 glucan 0.15% + Baism 2.85% and $\beta$-1,3 glucan 0.30% + Baism 2.70% of diets as a dry-matter(DM) basis were prepared. Three replicate groups of fish averaging 9.2 $\pm$ 0.1g (Mean $\pm$ SD) were randomly distributed in each aquarium as a group of 15 fish and fed one of eight experimental diets for seven weeks. After the feeding trial, $\beta$-1,3 glucan 0.10% + Baism 0.90%, $\beta$-1,3 glucan 0.10% + Baism 1.90% diets had a higher weight gain (WG), feed efficiency(FE), specific growth rate(SGR) and protein efficiency ratio(PER) than did fish fed 0%, $\beta$-1,3 glucan 0.05% + Baism 0.45%, $\beta$-1,3 glucan 0.05% + Baism 0.95%, $\beta$-1,3 glucan 0.15% + Baism 2.85% and $\beta$-1,3 glucan 0.30% + Baism 2.70% (P<0.05). however, there was no significant difference among fish fed $\beta$-1,3 glucan 0.05% + Baism 0.45%, $\beta$-1,3 glucan 0.05% + Baism 0.95%, $\beta$-1,3 glucan 0.15% + Baism 2.85% and $\beta$-1,3 glucan 0.30% + Baism 2.70%(P>0.05). and $\beta$-1,3 glucan 0.10% + Baism 0.90% diets had a higher peak value of CL(Chemiluminescence) and lysozyme activity, than did fish fed the other diets (P<0.05). These results indicated that dietary sipplementation of $\beta$-1, 3 glucan and Baism affected growth, feed efficiency, specific growth rate, protein efficiency ratio, Peak value of CL and Lysozyme activity, and the optimum dietary supplementation level of $\beta$-1, 3 glucan and Baism as a feed additive could be approximately $\beta$-1, 3 glucan 0.10% + Baism 0.90% of diet in juvenile olive flounder (Paralichthys olivaceus).

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Effect of $\beta$-glucan Extracted from Youngji Mushroom on the Growth Performance of Weaning Pigs (영지버섯에서 추출한 $\beta$-glucan 이 자돈의 생산능력에 미치는 영향)

  • Kim, Jong-Duk;Shim, Keum-Seob;Choi, Nag-Jin;Kim, Ji-Hoon;Kim, Yong-Hyun;Kwon, Hyun-Jung;Kim, Sun-Ki;Han, Man-Deuk
    • Korean Journal of Organic Agriculture
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    • v.18 no.3
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    • pp.401-418
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    • 2010
  • This experiment was selected a $\beta$-glucan producing mushroom strain and developed industrial media, and used to $\beta$-glucan as an alternative for antibiotics in weaned pigs. Yields of mycelial biomass and extracellular $\beta$-glucan from Youngji (Ganoderma lucidum) mushroom was 8.52g/L and 4.49g/L respectively. Also, we prepared optimum formula for mushroom cultivations. A total of 144 pigs ($8.6{\pm}0.9$ kg average body weight, weaned $20{\pm}3$ days of age) were allotted to 4 different treatment groups and replicated 4 times with 8 pigs per replicate in randomized complete block design. Treatments were T1) NC (negative control, basal diet), T2) PC (positive control, basal diet+0.255% antibiotics), T3) NC+0.2% $\beta$-glucan and T4) PC+0.2% $\beta$-glucan. The T2 and T4 treatments were significantly higher in feed efficiency by antibiotics group (p<0.05), however, there was no significant differences in terms of average daily gain (ADG) and average daily feed intake (ADFI) during phase I (0~14 days). In phase II (15~28 days), Pigs fed with antibiotics and $\beta$-glucan (T4) had greater ADG than other treatments (p<0.05), while no differences were observed in ADFI and feed efficiency. During the whole experiment period, the ADG of T4 treatment was higher than other treatments. Pigs fed with $\beta$-glucan (T3 and T4) had greater diarrhea score and moisture content than other treatments (p<0.05). Pigs fed with $\beta$-glucan (T3 and T4) had greater moisture content than other treatments (p<0.05). However, there was no significant differences in diarrhea score and mortality of weaned pigs. There was marginal reductions in feed cost measured feed cost per weight gain used in antibiotics and $\beta$-glucan added diet during phase I. In the second phase, the treatment supplemented with antibiotics had a significantly lower feed cost per weight gain compared to the other treatments. The results from these experiments suggests that $\beta$-glucan is likely able to improve the growth performance, and reduce feed cost although they do not have similar effects like antibiotics in weaning pigs.

Effect of β-Mannanase and α-Galactosidase Supplementation to Soybean Meal Based Diets on Growth, Feed Efficiency and Nutrient Digestibility of Rainbow Trout, Oncorhynchus mykiss (Walbaum)

  • Yigit, Nalan Ozgur;Koca, Seval Bahadir;Isil, Behire;Diler, Ibrahim
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.5
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    • pp.700-705
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    • 2014
  • A 12-week feeding trial was conducted with 87 g rainbow trout to evaluate the effects on growth performances, feed efficiency and nutrient digestibility of adding ${\beta}$-mannanase and ${\alpha}$-galactosidase enzymes, solely or in combination. Seven diets were prepared by adding ${\beta}$-mannanase, ${\alpha}$-galactosidase and mixed enzyme at two different levels (1 g/kg and 2 g/kg) to control diet (without enzyme) including soybean meal. Mixed enzymes (1 g/kg, 2 g/kg) were prepared by adding ${\beta}$-mannanase and ${\alpha}$-galactosidase at the same doses (0.5+0.5 g/kg and 1+1 g/kg). At the end of the experiment, addition of ${\beta}$-mannanase, ${\alpha}$-galactosidase and mixed enzyme to diet containing 44% soybean meal had no significant effects on growth performance and gain:feed (p>0.05). In addition, adding ${\beta}$-mannanase, ${\alpha}$-galactosidase and mixed enzyme in different rations to trout diets had no affect on nutrient digestibility and body composition (p>0.05).

Factors influencing on the Quality of Life in Older Adults after Total Knee Replacement: The Relevance to Pain, Range of Motion, Depression, Social Support and Sense of Coherence (슬관절전치환술을 받은 노인의 삶의 질 영향 요인: 통증, 관절가동범위, 우울, 사회적 지지, 통합성과의 관련성)

  • Yu, Mijin;Kim, HeeJung
    • Research in Community and Public Health Nursing
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    • v.28 no.4
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    • pp.494-503
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    • 2017
  • Purpose: This study is to grasp factors influencing the quality of life in older adults after total knee replacement. Methods: This study was conducted with 165 older adults who had TKR at four orthopedic hospitals in D city. Data were analyzed using one-way ANOVA, independent t-test, Pearson's correlation, and stepwise multiple linear regression with SPSS 19.0 software. Results: Pain and depression were negatively correlated with range of motion, social support, while sense of coherence was positively correlated with quality of life. Sense of coherence (43%, ${\beta}=.40$), pain (8%, ${\beta}=-.30$), and depression (3%, ${\beta}=-.20$) on the Physical Component Summary in the quality of life have significant explanatory power of 54%. Sense of coherence (49%, ${\beta}=.44$), social support (6%, ${\beta}=.25$), and depression (3%, ${\beta}=-.22$) on the Mental Component Summary in the quality of life have significant explanatory power of 58%. Conclusion: This study suggests developing a program to improve the quality of life in older adults who had TKR, considering factors such as sense of coherence.