• Title/Summary/Keyword: beta-HAD

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Effects of Mugwort, Onion and Polygalae Radix on the Intestinal Environment of Rats (쑥, 양파 및 원지가 흰쥐의 장내환경에 미치는 영향)

  • Lee, Seon-Hwa;Woo, Sun-Ja;Koo, Young-Jo;Shin, Hyun-Kyung
    • Korean Journal of Food Science and Technology
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    • v.27 no.4
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    • pp.598-604
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    • 1995
  • The contents in large intestine of Sprague Dawley rats fed polygalae radix(Polygala tennuifolia), onion(Allium cepa) and mugwort(Artemisia asiatica)-supplemented diets for 14 days were analysed for changes of major intestinal microflora, activities of ${\beta}-glucosidase\;and\;{\beta}-glucuronidase$ and amounts of putrefactive products such as indole and volatile basic nitrogen. The rats having ingested $5%{\sim}10%$ mugwort water or ethanol extract-supplemented diets showed a significant increase in intestinal bifido-bacteria and a decrease in clostridia and E. coli (p<0.05). And 10% onion juice group also showed a similar beneficial microflora change. In 5% mugwort powder-supplemented group, ${\beta}-glucosidase\;and\;{\beta}-glucuronidase$ activities in the intestinal contents were lowered, but the changes were not significant. Indole contents and pH in this group were significantly low compared with that of control (p<0.05). However, the activities of ${\beta}-glucosidase$ in 5% polygalae radix water extract and 10% onion juice-supplemented group and ${\beta}-glucuronidase\;in\;5%{\sim}10%$ mugwort water and ethanol extract-supplemented group were significantly higher than those of control (p<0.05). The intestinal indole contents of rats were significantly increased by feeding diet with water extract of polygalae radix and ethanol extract of mugwort which had brought comparatively large amount of protein in intestine (p<0.05). However, polygalae radix, onion, and mugwort-supplemented group had no effect on volatile basic nitrogen.

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The Influence of Foodservice Industry Culinary Staff's Workplace Harassment in Organizational Silence, Counterproductive Work Behavior and Turnover Intent: Focus on Moderating Effects on Gender and Staff's Job Status (외식산업 조리종사원의 직장 내 괴롭힘이 조직침묵, 반생산적 행동 및 이직의도에 미치는 영향: 성별과 고용형태의 조절효과 검증)

  • Kim, Young-Joong
    • Culinary science and hospitality research
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    • v.23 no.3
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    • pp.15-28
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    • 2017
  • The purpose of this study is to examine the influence of workplace harassment in foodservice industry culinary staff's on organizational silence, counterproductive work behavior and turnover intent. Based on total 234 samples obtained from empirical research, this study tested the reliability and fitness of the research model and verified a total of 5 hypotheses using the AMOS program. Using a structural equation model (SEM), hypothesized relationships in the model were tested simultaneously. The proposed model provided an adequate fit the data, $x^2=75.936$ (p<.001), df=41, CMIN/DF=1.852, GFI=.946, AGFI=.913, NFI=.914, TLI=.944, CFI=.958, RMSEA=.060. The model's fit, as indicated by these indexes, was deemed satisfactory, thus providing a good basis for testing the hypothesized paths. The SEM showed that the relational workplace harassment (${\beta}=.957$) had a positive significant influence on organizational silence, organizational silence (${\beta}=.934$) had a positive significant influence on counterproductive work behavior. Also, counterproductive work behavior (${\beta}=.815$) had a positive significant influence on turnover intention. The moderating effects on gender and job status did not show significant effect. Limitations and future research directions are also discussed.

Influencing Factors of Marital Intimacy and Sexual Knowledge on Parental Efficacy of Mothers with Preschoolers on Child Sexual Education (학령전기 아동을 둔 어머니의 자녀 성교육에 대한 부모효능감 영향 요인)

  • Jo, Myoung-Ju;Lim, Kyoun-Joo;Choi, Eun Joung
    • Journal of Korean Public Health Nursing
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    • v.32 no.2
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    • pp.181-193
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    • 2018
  • Purpose: This paper reports the results of a descriptive study that aims to identify the factors that affect the parental efficacy of mothers with preschoolers on child sexual education. Methods: Using a structured questionnaire, data were collected from 189 mothers who had preschoolers in four kindergartens in Seoul and Gyeonggi Province from June to August, 2015. Results: The factors that had an effect on the parental efficacy of mothers with preschoolers on child sexual education were marital intimacy (${\beta}=.32$, p<.001), the degree of perceived sexual knowledge (${\beta}=.29$, p<.001), sexual education experience from one's parents (${\beta}=.21$, p=.001), and educational level (${\beta}=.14$, p=.021); the explanatory power was 28.9%. Conclusion: The factors affecting the parental efficacy of mothers with preschoolers on child sexual education were marital intimacy, subjective sexual knowledge, sexual education experience from one's parents, and the educational level. In developing a sex education program for mothers with preschoolers, it is necessary to reflect the contents that can enhance marital intimacy. In addition, it is necessary to develop a differentiated mother sex education program considering her demographic characteristics and career in sexual health.

The Inhibitory Effects of Lactose-${\beta}$-sitosterol on the Inflammatory Responses of HMC-1 Cells and EoL-1 Cells

  • Yang, Eun-Ju;Kim, In-Sik
    • Biomedical Science Letters
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    • v.17 no.3
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    • pp.217-223
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    • 2011
  • ${\beta}$-sitosterol glucoside exists in a variety of plants and have anti-tumor, anti-microbial, and immunomodulatory activities. Mast cells and eosinophils play important roles in a variety of inflammatory diseases, specifically asthma and atopic dermatitis. In the present study, we used lactose-${\beta}$-sitosterol (L-BS) and investigated the effect of L-BS on inflammatory responses of the human mast cell line, HMC-1 and the human eosinophilic leukemia cell line, EoL-1. In HMC-1 cells, L-BS significantly inhibited cell migration in response to stem cell factor without cytotoxicity. However, the mRNA expression of CC chemokine receptors (CCRs), including CCR1-5, were not altered after L-BS treatment in HMC-1 cells. LPS-induced IL-4 production was also suppressed by L-BS in a dose-dependent manner. In EoL-1 cells, the concentration ranging from 0.1 ${\mu}M$ to 10 ${\mu}M$ of L-BS had no cytotoxicity and had no effect on mRNA expression of major protein-mediators derived from activated eosinophils. However, 100 ${\mu}M$ of L-BS induced the apoptosis of EoL-1 cells in a time-dependent manner. This finding indicates the possibility of L-BS as a potential therapeutic molecule in inflammatory diseases and may contribute to the need to improve current therapeutic drugs.

The Prediction Factor on Organizational Commitment of the Nurse (간호사의 조직몰입 예측요인)

  • Moon, Sook-Ja;Han, Sang-Sook
    • The Journal of Korean Academic Society of Nursing Education
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    • v.15 no.1
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    • pp.72-80
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    • 2009
  • Purpose: This study was designed to identify the prediction factors that influence nurses' organizational commitment. Method: The sample of this study consisted of 526 full-time nurses randomly picked at 19 general hospitals in Korea. The data was analyzed by computer using SPSS 15.0 for Pearson's correlation coefficient, and multiple regression analysis. Result: 1) According to general characteristics, nurses' organizational commitment levels among the sample were significantly different in age, religion, social status, marital status, clinical career, and department satisfaction. 2) Level of nurses' organizational commitment was average 2.70, job satisfaction 2.91, burnout 3.03, empowerment 3.36, autonomy 2.93, and self-efficacy 3.51. 3) Nurses' organizational commitment had significant positive correlations with job satisfaction, empowerment, self-regulation, social support, self-efficacy, clinical career, and personnel movement experience. On the other hand, it had significant negative correlations with occupational stress, burnout, and age. 4) The prediction factors which influence Nurses' organizational commitment were job satisfaction($\beta$=.405), burnout($\beta$=-.282), self-regulation($\beta$=.171), clinical career($\beta$=.135). These factors were approximately 49.6% reliable in explaining nurses' organizational commitment. Conclusion: These results can be used to develop hospitals' management strategies for increasing organizational commitment effectiveness and nursing productivity.

Effects of Attitude, Social Influence, and Self-Efficacy Model Factors on Regular Mammography Performance in Life-Transition Aged Women in Korea

  • Lee, Chang Hyun;Kim, Young Im
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.8
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    • pp.3429-3434
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    • 2015
  • Background: This study analyzed predictors of regular mammography performance in Korea. In addition, we determined factors affecting regular mammography performance in life-transition aged women by applying an attitude, social influence, and self-efficacy (ASE) model. Materials and Methods: Data were collected from women aged over 40 years residing in province J in Korea. The 178 enrolled subjects provided informed voluntary consent prior to completing a structural questionnaire. Results: The overall regular mammography performance rate of the subjects was 41.6%. Older age, city residency, high income and part-time job were associated with a high regular mammography performance. Among women who had undergone more breast self-examinations (BSE) or more doctors' physical examinations (PE), there were higher regular mammography performance rates. All three ASE model factors were significantly associated with regular mammography performance. Women with a high level of positive ASE values had a significantly high regular mammography performance rate. Within the ASE model, self-efficacy and social influence were particularly important. Logistic regression analysis explained 34.7% of regular mammography performance and PE experience (${\beta}=4.645$, p=.003), part-time job (${\beta}=4.010$, p=.050), self-efficacy (${\beta}=1.820$, p=.026) and social influence (${\beta}=1.509$, p=.038) were significant factors. Conclusions: Promotional strategies that could improve self-efficacy, reinforce social influence and reduce geographical, time and financial barriers are needed to increase the regular mammography performance rate in life-transition aged.

The Effects of Verbal and Nonverbal Communication of Service Providers in the Family Restaurant on the Customers' Trust and Satisfaction (패밀리레스토랑 서비스 제공자의 언어적, 비언어적 커뮤니케이션이 고객 신뢰 및 만족도에 미치는 영향)

  • Jung, Hyo-Sun;Yoon, Hye-Hyun
    • Korean journal of food and cookery science
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    • v.26 no.3
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    • pp.272-280
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    • 2010
  • The purpose of this study was to understand the interrelationships between the customers' perception of verbal and nonverbal communication, customers' trust and satisfaction in the family restaurant. Based on a total of 310 samples that were obtained from empirical research, this study reviewed the reliability and fitness of the research model and verified a total of 2 hypotheses using the Amos program. The hypothesized relationships in the model were tested simultaneously using a structural equation model(SEM). The proposed model provided an adequate fit to the data, $X^2$=953.126(df=417), p<.001, CMIN/df 2.286, GFI .831, NFI .889, IFI .934, TLI .926, CFI .934. The results showed that employees' verbal communication ($\beta$=.264), paralanguage ($\beta$=.172) and physical appearance ($\beta$=.120) among the nonverbal communications had a significant positive effect on the customers' trust. In addition, the customers' trust ($\beta$=.644) had a significant positive effect on the customer satisfaction. Limitations and future research directions are also discussed.

Modification of Sweet Potato $\beta$-Amylase with Periodate-Oxidized Soluble Starch (과요소산 산화 전분에 의한 고구마 $\beta$-아밀라제의 수식)

  • 안룡근;지의상
    • The Korean Journal of Food And Nutrition
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    • v.3 no.2
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    • pp.123-132
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    • 1990
  • Sweet potato $\beta$-amylase is a tetrameric enzyme consisting of four identical polypeptide chains with a molecular weight of 5.6$\times$104, though most of the other $\beta$-amylases are monomeric enzymes. But, the relationship between subunit structure and catalytic function of the enzyme is not known. This study was done to know what the function of the subunit structure of the enzyme is. We obtained the monomer from the enzyme by the treatment of SDS, alkali pH buffer and urea. But the monomer had not activity. We tried to prepare the active monomer from the enzyme by the modification with periodate-oxidized soluble starch , In the result, we succeeded in isolating an active monomer as an oxidized soluble starch-conjugated form The active monomer had 57% of the original activity, 13.2% of the sugar and the molecular weight was estimated to be 5.4$\times$104. This results suggest that the tetrameric form of the enzyme is a most stable one and exists in nature, and the subunit structure of the enzyme Plays an important role in stabilization but not catalytic function.

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Structure of the Starch-Binding Domain of Bacillus cereus $\beta-Amylase$

  • Yoon, Hye-Jin;Akira, Hirata;Motoyasu, Adachi;Atsushi, Sekine;Shigeru, Utsumi;Bunzo, Mikami
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.619-623
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    • 1999
  • The C-terminal starch-binding domain of Bacillus cereus $\beta$-amylase expressed in Escherichia coli was purified and crystallized using the vapor diffusion method. The crystals obtained belong to a space group of $P3_2$ 21 with cell dimensions, a=b=60.20${\AA},\; c=64.92{\AA},\; and \; \gamma = 120^{\circ}$ The structure was determined by the molecular replacement method and refined at 1.95 ${\AA}$, with R-factors of 0.181. The final model of the starch-binding domain comprised 99 amino acid residues and 108 water molecules. The starch-binding domain had a secondary structure of two 4-stranded antiparallel p-sheets similar to domain E of cyclodextrin glucanotransferase and the C-terminal starch-binding domain of glucoamylase. A comparison of the structures of these starch-binding domains revealed that the separated starch-binding domain of Bacillus cereus $\beta-Amylase$had only one starch-binding site (site 1) in contrast to two sites (site 1 and site 2) reported in the domains of cyclodextrin glucanotransferase and glucoamylase.

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Control Effect of Illness Perception on Depression and Quality of Life in Patients with Hemodialysis: Using Structural Equation Modeling (혈액투석 환자의 우울과 삶의 질에서 질병인식의 통제효과)

  • Kim, Sisook;Ryu, Eunjung
    • Journal of Korean Biological Nursing Science
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    • v.20 no.4
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    • pp.221-227
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    • 2018
  • Purpose: This study aimed to evaluate the effect of illness perception on depression and quality of life in patients with hemodialysis. Methods: This is a cross-sectional study using structural equation modeling. Depression, quality of life and cognitive and emotional illness perceptions were measured by the Center for Epidemiologic Studies Depression-10 (CESD-10), Short Form-36 version 2 (SF-36v2), and Brief Illness Perception Questionnaire (BIPQ) in 272 hemodialysis patients. Results: Prevalence of depression was 76.1%, and higher in female patients on hemodialysis. Cognitive illness perception had effect on depression and quality of life, while emotional illness perception had effect only on depression. Quality of life was explained 69.6% through depression (${\beta}=-.74$, t= -8.17) and cognitive illness perceptions (${\beta}=.21$, t= 3.06), while depression was explained 40.5% through cognitive (${\beta}=-.34$, t= -5.99) and emotional (${\beta}=.59$, t= 9.37) illness perceptions. Conclusion: In the inevitable choice of dialysis, illness perception could decrease depression and improve quality of life in hemodialysis patients.