• Horticultural Science & Technology
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• v.32 no.5
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• pp.577-583
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• 2014

Benzyladenine (BA, 99% purity), MaxCel$^{(R)}$ (1.9% BA), Fruitone (3.5% NAA), MaxCel$^{(R)}$ + Fruitone, a nd s imazine were applied postbloom as fruitlet thinning agents to mature 'Hongro' and 'Fuji' apple (Malus domestica Borkh.) trees. BA and MaxCel$^{(R)}$ were applied at $100mg{\cdot}L^{-1}$ a.i. while Fruitone at $0.1mg{\cdot}L^{-1}$ a.i. and simazine at $400mg{\cdot}L^{-1}$ a.i. All PGRs were applied at 8 days after full bloom (DAFB, 6 mm fruit diameter) in both cultivars, while simazine was treated twice at 7 and 14 DAFB. In 'Hongro', the number of total fruit set per flower cluster in terminal buds was 1.67, 1.84, and 1.81 in MaxCel$^{(R)}$ + F ruitone, MaxCel$^{(R)}$, and simazine applications, respectively, when compared with 2.35 of water control. These reductions in fruit set were mainly attributed to the increased ratio of defruited clusters by the thinning agents. In 'Fuji' apple, the number of total fruit set per flower cluster in terminal buds was 1.29, 1.60, and 1.76 in MaxCel$^{(R)}$ + Fruitone, Fruitone, and MaxCel$^{(R)}$, respectively, when compared with 2.56 of water control in 'Fuji' apple. The addition of Fruitone to the MaxCel$^{(R)}$ promoted the thinning efficacy in both cultivars, compared to MaxCel$^{(R)}$ only. The thinning efficacies were similarly observed with lateral flowers in both cultivars. A significant increase of fruit weight by the postbloom thinning treatments was observed only in the BA application in 'Hongro', while the effect was observed in BA and MaxCel$^{(R)}$ in 'Fuji'. While the soluble solids content increased in the BA, MaxCel$^{(R)}$ and MaxCel$^{(R)}$+Fruitone treatments in both cultivars, other fruit quality attributes were not affected by the application of post-bloom thinning agents.

• Horticultural Science & Technology
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• v.16 no.1
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• pp.27-29
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• 1998

Artificial pollination (AP) and gibberellin A4+7 plus benzyladenine (promalin) were applied alone and together. AP was applied at 10% flowering time with 'Senshu' pollen (Malus domestica cv. Senshu). 12mg/L promalin was applied at 0, 10 and 20 days after falling of central flowers, respectively. In promalin treatment with or without AP application methods, fruit length, weight and length/diameter (L/D) were higher than those of control and AP. However, in AP and AP+ promalin application, the number of seeds and seed weight were higher than those of control and promalin. In AP+ promalin treatment, 78.6% fruits showed their uniform fruit shape and so significantly enhanced fruit uniformity compared to other treatments. Also cortex and core thickness of fruits were greater at the apex than that of other treatment. Fruit with L/D ratio over 0.87 were highly produced by applications of AP+promalin and promalin than control and AP only. 'Fuji' apples in good shape can be produced by using of AP + promalin together.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.315-319
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• 2001

The study was carried out to develop a simple and efficient system to regenerate plants from cotyledon and hypocotyl tissues of Chinese cabbage (Brassica campestris L. ssp. pekinensis cv Seoul). Among the various combinations of naphthalene acetic acid (NAA) and 6-benzyladenine (BA) tested, the best shoot induction medium for cotyledon, with 2.67 shoots per explants, contained $2.0mg{\cdot}L^{-1}$ NAA, $1.0mg{\cdot}L^{-1}$ BA and $16.7mg{\cdot}L^{-1}$ $AgNO_3$. The shoot induction medium with $1.0mg{\cdot}L^{-1}$ NAA, $5.0mg{\cdot}L^{-1}$ BA and $16.7mg{\cdot}L^{-1}$ $AgNO_3$, was best for shoot induction from hypocotyl explants, with 1.87 shoots per explants. After shoot induction, regenerated shoots were excised and rooted on rooting medium. Rooted plantlets were then hardened in the high humidity growth chamber and transplanted to pots, and then grown in the greenhouse. Regenerated plants appeared phenotypically normal and there were no changes in chromosome number.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.275-279
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• 2005

The fruiting habit pattern of white-spine cucumber, whose fruits are usually borne on the lateral branches, is different from domestic ones. The cucumber production far export has been focused on how to promote lateral shooting and how to increase the number of lateral branches. As the growing season of the exporting cucumber is confined to winter season, low temperature and weak light are limiting factors to lateral shooting. This study was conducted to investigate the effect of foliar application of growth regulator for induction of lateral shoot in white-spine cucumber. foliar application of growth regulator were focused on concentration and application time based on number of leaves. The visible damages were observed in applied leaves by $30mg{\cdot}L^{-1}$ BA(benzyladenine) in retarding culture, but no significant by $10mg{\cdot}L^{-1}$ BA in case of semi-forcing culture. The number of available lateral shoots were greater in applied plant with $10mg{\cdot}L^{-1}$ BA than that of $30mg{\cdot}L^{-1}$ BA, in applied plants at 10th and 15th loaves than that of applied plants at 5th leaves.

• Korean Journal of Environmental Agriculture
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• v.16 no.3
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• pp.227-232
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• 1997

Visible injury appeared 7 days after ultraviolet-B(UV-B) irradiation, but did not show any significant decline of growth in cucumber plant. However the growth of the first leaves of fertilized plants was suppressed by UV-B irradiation. Especially the most effective growth retardiation appeared when supplied with nitrogen rather than phosphate and potassium. These results suggest that UV-B may play an important role in inhibiting nitrogen metabolism. Therefore we examined the effect of activity of nitrate reductase, and found that the nitrate reductase activity of the first leaves was increased by UV-B irradiation for 7 days and fertilization. We examined the effect of plant hormone on the inhibition of growth in the first leaves. Benzyladenine promoted the growth of discs excised from the first leaves by fertilization and without UV-B, but did not promote the growth of leaf discs from UV-B irradiated plants. We conclude that the UV-B-induced decrease in the growth of the first leaves could be related to reduction in sensitivity to plant hormones.

• FLOWER RESEARCH JOURNAL
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• v.18 no.2
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• pp.87-92
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• 2010

This research was aimed to extend the vase life, improve the cut flower quality and promote flowering rate of bulbous cut Iris 'Blue Magic'. Three different concentrations of 1-MCP ranging from 250 to $750nL{\cdot}L^{-1}$ were treated on cut Iris flowers for the vase life elongation. Several effects of 1-MCP treatment has shown such as early flowering as one day but vase life showed no significant differences comparing to the untreated control. Flowering rate was 75% or more in the treated ones as compare to 64.2% in untreated control. Especially, in a treatment with $250nL{\cdot}L^{-1}$ 1-MCP for 12 hours it showed all flowering. In many post-harvest experiments of cut flowers, ethylene production from flower organ has considered as most critical factor on vase life. Ethylene production from flower organ was measured by gas chromatography (GC) and it showed that there is no direct relation between flower longevity and emission of the ethylene gas in cut Iris. In comparison with the untreated control, ethylene generation rather seems to be increased as 1-MCP treatment increased. There was a synergy effect when 1-MCP and BA applied simultaneously in which flowering and vase life were fastened and also extended, respectively. Therefore, treatment of 1-MCP for cut Iris at flower early harvest is able to improve the flower quality both by diminishing non-flowering rate and by extended flower longevity.

• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.106-113
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• 2019

The objective of this study was to carry out treatment of various plant hormones in order to determine morphological and genetic variation degree of tissue-cultured strawberry. The cultivar used in this experiment was 'Goha' and 'Seolhyang', the plant hormones used for experiment were benzyladenine (BA), N-(2-Chloro-4 pyridyl)-N'-phenylurea (CPPU) and thidiazuron (TDZ), and the concentrations were 0.5, 1.0, 2.0, $4.0mg{\cdot}L^{-1}$ with each hormone. The BA treatment of the proliferation efficiency of tissue-cultured strawberry 'Goha' and 'Seolhyang' was the highest. When processing BA, CPPU and TDZ, morphological variation and genetic variation happened in strawberry 'Goha' and 'Seolhyang', especially, the variations appeared highly in CPPU treatment. The genetic variation in 'Goha' appeared at the concentration more than BA $0.5mg{\cdot}L^{-1}$ as 1.1%, appeared at the concentration of CPPU $0.5mg{\cdot}L^{-1}$ as 15.3%, and at the concentration of TDZ $2.0mg{\cdot}L^{-1}$ as 1.2%. The genetic variation in 'Seolhyang' appeared at the concentration of BA $4.0mg{\cdot}L^{-1}$ as 2.3%, and at the concentration of CPPU $0.5mg{\cdot}L^{-1}$ as 14.3%. Therefore, CPPU should not be treated during strawberry tissue culture, and BA and TDZ should be treated at low concentration.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.5
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• pp.352-355
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• 2002

Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration. Murashige and Skoog(MS) and N6 media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin were used for callus induction. Percentage of callus induction showed significant among the combinations of plant growth regulators. Callus induction showed high efficiency in medium containing 3 mg/$\ell$ of 2,4-D. The high frequency of callus induction was obtained in Gwiri37. For plant regeneration, calli induced from mature embryos were transferred onto MS and N6 media supplemented with combinations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) for 5 weeks. Percentage of plant regeneration showed high in MS medium containing 0.2 mg/$\ell$ of NAA and 1 mg/$\ell$ of BA. The callus initiation medium affected the subsequent plant regeneration. Treatment with 3 mg/$\ell$ of 2,4-D, and 3 mg/$\ell$ of 2,4-D and 3 mg/$\ell$ of kinetin in callus induction media showed high frequency for plant regeneration. Plant regeneration frequency among the genotypes showed significant. Especially, Gwiri37 showed high regeneration frequency. Regenerated shoots were treated with 200, 350 and 500 mg/$\ell$ of indole-3-butyric acid (IBA) transferred onto half-strength MS medium without plant growth regulators. Treatment of shoots with IBA induced root formation rapidly.

• Plant Biotechnology Reports
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• v.4 no.2
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• pp.173-178
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• 2010

An efficient protocol for shoot regeneration was developed for sesame (Sesamum indicum L.) internodes using the transverse thin cell layer (tTCL) culture method. The frequency of shoot regeneration and the number of adventitious buds produced from regenerated shoots depend significantly on explant age, thickness of the tTCL sections, and the phytohormones supplemented to the culture medium. A combination of 6-benzyladenine (2.0 $mg\;l^{-1}$) and a-naphthaleneacetic acid (0.5 $mg\;l^{-1}$) was found to be the best phytohormone combination for shoot bud induction, with the maximum number of shoots obtained when the tTCL sections were 0.5-1.0 mm thick and derived from 4- to 6-week-old seedlings of sesame. Well-developed shoots were rooted on MS medium without phytohormones, and 80% of the regenerated plantlets were successfully established in soil.

• Plant Biotechnology Reports
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• v.4 no.2
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• pp.129-138
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• 2010

An efficient transformation system for high-throughput functional genomic studies of kiwifruit has been developed to overcome the problem of necrosis in Actinidia arguta explants. The system uses Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pART27-10 to inoculate leaf strips. The vector contains neomycin phosphotransferase (nptII) and ${\beta}$-glucuronidase (GUS) (uidA) genes. A range of light intensities and different strengths of Murashige and Skoog (MS) basal salt media was used to overcome the problem of browning and/or necrosis of explants and calli. Callus browning was significantly reduced, resulting in regenerated adventitious shoots when the MS basal salt concentration in the culture medium was reduced to half-strength at low light intensity ($3.4\;{\mu}mol\;m^{-2}\;s^{-1}$) conditions. Inoculated leaf strips produced putative transformed shoots of Actinidia arguta on half-MS basal salt medium supplemented with 3.0 $mg\;l^{-1}$ zeatin, 0.5 $mg\;l^{-1}$ 6-benzyladenine, 0.05 $mg\;l^{-1}$ naphthalene acetic acid, 150 $mg\;l^{-1}$ kanamycin and 300 $mg\;l^{-1}$ $Timentin^{(R)}$. All regenerated plantlets were deemed putativ transgenic by histochemical GUS assay and polymerase chain-reaction analysis.