• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3437-3445
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• 2016

Background: There is an increasing concern in the role of microRNA (miRNA) in the pathogenesis of bone metastasis (BM) secondary to prostate cancer (CaP). In this exploratory study, we hypothesized that the expression of vinculin (VCL) and chemokine X3C ligand 1 (CX3CL1) might be down-regulated in clinical samples, most likely due to the post-transcriptional modification by microRNAs. Targeted genes would be up-regulated upon transfection of the bone metastatic prostate cancer cell line, PC3, with specific microRNA inhibitors. Materials and Methods: MicroRNA software predicted that miR-21 targets VCL while miR-29a targets CX3CL1. Twenty benign prostatic hyperplasia (BPH) and 16 high grade CaP formalin-fixed paraffin embedded (FFPE) specimens were analysed. From the bone scan results, high grade CaP samples were further classified into CaP with no BM and CaP with BM. Transient transfection with respective microRNA inhibitors was done in both RWPE-1 (normal) and PC3 cell lines. QPCR was performed in all FFPE samples and transfected cell lines to measure VCL and CX3CL1 levels. Results: QPCR confirmed that VCL messenger RNA (mRNA) was significantly down-regulated while CX3CL1 was up-regulated in all FFPE specimens. Transient transfection with microRNA inhibitors in PC3 cells followed by qPCR of the targeted genes showed that VCL mRNA was significantly upregulated while CX3CL1 mRNA was significantly down-regulated compared to the RWPE-1 case. Conclusions: The down-regulation of VCL in FFPE specimens is most likely regulated by miR-21 based on the in vitro evidence but the exact mechanism of how miR-21 can regulate VCL is unclear. Up-regulated in CaP, CX3CL1 was found not regulated by miR-29a. More microRNA screening is required to understand the regulation of this chemokine in CaP with bone metastasis. Understanding miRNA-mRNA interactions may provide additional knowledge for individualized study of cancers.

• 대한중풍순환신경학회지
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• 제15권1호
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• pp.57-65
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• 2014

■ Objectives The purpose of this case report is to show an effect of korean medicine for patient with bilateral abducens nerve palsy. ■ Methods This patient was 58 year-old man who was diagnosed with bilateral abducens nerve palsy. The patient had Diabetes Mellitus, hypertension, benign prostatic hyperplasia and dyslipidemia as underlying diseases. The patient had symptoms of limited eye movement to lateral, diplopia, dizziness and mild headache. He was treated by korean medicine, containing electroacupuncture, pharmacoacupuncture, moxibustion and herb medicines during 13 days of hospitalization and following outpatient department. His eye movement was evaluated by the method of Scott and kraft and discomfort from his symptoms was scored by the Numerous Rating Scale. And other general conditions were measured. ■ Results After that period, patient's abduction deficit was improved and subjective discomfort from his symptoms was getting better. His other conditions were also better, compared with his admission. ■ Conclusion This study suggests that Korean medicine could have a therapeutic effect for bilateral abducens nerve palsy. So, It could help to relieve patient's symptoms and make general conditions better.

• 농촌의학ㆍ지역보건
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• 제25권2호
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• pp.265-273
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• 2000

농촌 지역 사회의 40대 이상의 남성 300명을 대상으로, 번역의 타당성과 신뢰도가 검증된 국문 I-PSS를 이용하여, 하부요로 증상의 유병률, I-PSS와 혈압의 관계를 조사하여 다음과 같은 결론을 얻었다. 1. I-PSS를 이용한 증상접수 8이상인 하부 요로증상의 유병률은 40, 50, 60 및 70대에서 각각 20.5, 32.2, 40.6 및 72.0%로 관찰되었으며 증상점수 20 이상의 중증군은 각각 0.82, 5.6, 11.0%로 미국, 일본에 비하여 높게 나타났다. 2. 평균 I-PSS와 연령과의 관계는 연령이 증가함에 따라 증가하였으며(r=0.29, p=0.0001), 무학인 군이 전문대 이상인 군에 비하여 I-PSS가 유의하게 높게 관찰되었다(p<0.05). 3. 평균 I-PSS와 배뇨만족도 사이에는 유의한 상관관계(r=0.63, p=0.0001)를 보였으나 I-PSS(평균 8.2)에 비해 상대적으로 불편감이 없는 배뇨만족도(평균 1.9)를 나타내었다. 4. 고혈압군의 평균 I-PSS는 7.85이었으며 정상혈압군의 평균 I-PSS는 8.39이었으나 I-PSS와 혈압과의 관계는 고혈압군과 정상 혈압군 간에 유의한 차이를 보이지 않았다(p>0.05).