• The Journal of Korean Society of Virology
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• v.30 no.1
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• pp.1-10
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• 2000

The nonstructural protein NSP4, encoded by gene 10 of rotavirus, has been shown to playa role in viral assembly and known to be an enterotoxin, causing diarrhea in mouse pups. NSP4 gene was cloned from CBNU-2 (virulent bovine rotavirus/diarrheic fecal sample) and CBNU-1 (cell-culture adapted bovine rotavirus/isolated from CBNU-2 and 75 times passaged on MA104 cells), respectively, by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced and compared. The sequence data indicated that the NSP4 genes of bovine rotavirus (BRV) were 751 bases in length and encoded one open reading frame of 175 amino acids beginning at base 42 and terminating at base 569. Differences in nucleotide sequence between CBNU-2 and CBNU-1 were observed at 6 positions (base 274, 296, 391, 394, 396 and 579). NSP4 gene of BRV exhibited a high degree of nucleotide (90% and 94%) and amino acid sequence (91% and 97%) homology with those of SA11 and UK but a low degree of nucleotide (77% and 79%) and amino acids sequence (81% and 85%) homology with those of Wa and OSU.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.13 no.2
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• pp.47-53
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• 2004

High speed precision machines have been introduced to the CNC industry in order to improve productivity, shorten the appointed date of delivery and reduce the prime cost. High speed machines have more functions then general machines, and they were proved in performance. The production and sales of the high speed machines have been increased not only in domestic market but also all over the world. Accordingly, machines are faster, there are lots of problems to be solved. One of the most difficult problems is the thermal displacement on the main spindle due to generated heat while the spindle is rotated in high speed. Since the thermal displacement directly effects the quality of the machined parts, utmost efforts to minimize the thermal displacement have to be given from the beginning of designing machines. In practice, variety of methods are attempted and practiced to minimize the thermal displacement such as design of symmetrical frame, adoption of high speed bearings, application of compensation system using non-contact sensor and use of forced circulating lubrication system with oil cooler. Even if these variable methods have been practically used in the industrial field, generated heat has not been perfectly prevented. Hence, in this paper, the characteristics of thermal displacement were investigated when several kinds of oil were tested for a high speed machine with forced circulating lubrication system within the same atmosphere and under the same conditions.

• Journal of Families and Better Life
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• v.19 no.2
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• pp.67-82
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• 2001

This paper serves to analyze discourses on concubine at the beginning of the modernization era or during the enlightenment period of Korea. For this analysis, the estimated time frame of the enlightenment period will be from 1860 through the time when Korea was annexed into Japan, which was in 1910. The discourses appeared in newspapers and Shinsoseol which are the text of this study have been analyzed with the qualitative research technique. The major conclusions that are derived from the study are as follows: First, intellectuals during enlightenment period of Korea, criticizing concubinage, tired to establish the monogamous relationship and they argued that it should be the prior condition for Korea to enter into the civilized society. Second, the concubinage was criticized in the point of the newly established view. The criticism was on double sex ethics, applying different ethical standards to men and women and the prohibition of wifes jealousy of concubine. Third, intellectuals during enlightenment period of Korea were tried to do away with concubinage by enforcing the discrimination between wife and concubine. But the efforts resulted in making concubinage personal problems. Also consciousness based confucian ethics including female virtues was forced to be followed in ordinary affairs while concubinage was criticized in the point of the view of western ethics based consciousness. The huge differences between two ethics consciousness resulted in serious conflicts in family.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.05a
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• pp.187-190
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• 2009

Recently, automotive companies have invested in vehicle weight reduction and clean car development because of oil price rises and environmental problems. In particular, USA car makers have developed the vehicle spending 1 liter per 34km complying with PNGV(Partnership for a new generation of vehicle) and Europe car makers have developed the vehicle spending 3 liters per 100km. The USA government announced "The green car policy" in order to boost production of more fuel effective cars in 2009. According to the policy, it will be restricted to sell the car which spends more than 1 liter per 14.9km by 2020. To satisfy the current situations on automotive market, hydroforming technology has widely adapted vehicle structures such as engine cradle, chassis frame, A pillar, radiator support, etc. However, automotive companies have to consider formability and performance to improve and maximize the benefit from this technology in advance of detail design. The paper deals with one of the vehicle weight reduction methods using tube hydroforming technology and platform commonality in front suspension. FEA simulation is also introduced to evaluate hydro-formability and NVH performance at the beginning of design stage which is the best way to reduce the failure cost.

• Korean Journal of Veterinary Research
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• v.45 no.2
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• pp.207-214
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• 2005

The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene of avian rotavirus (AvRV-1, AvRV-2) was analyzed and expressed using baculovirus expression system. The sequence data indicated that the NSP4 gene of AvRV-1 and AvRV-2 were 727 bases in length, encoded one open reading frame of 169 amino acids beginning at base 41 and terminating at base 550, and had two glycosylation sites. Nucleotide sequences of NSP4 gene of AvRV-1 and AvRV-2 exhibited a high degree of homology ($88.1{\pm}7.6%$) with avian rotaviruses, namely Ty1, Ty3 and PO-13. Phylogenetic analysis showed that AvRV-1 and AvRV-2 belonged to genotype NSP4[E], which is widely found in group A avian rotaviruses. The baculovirus-expressed NSP4 migrated at 20-28 kDa and reacted with NSP4-specific antiserum by FA and Western blot. Furthermore, it was found to be a glycoprotein by using tunicamycin, which is a specific inhibitor of N-linked glycosylation.

• Computers and Concrete
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• v.25 no.2
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• pp.167-179
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• 2020

Optimal design of structures against earthquake loads is often limited to reduce initial construction costs, while the cost induced to structures during their useful life may be several times greater than the initial costs. Therefore, it is necessary to consider the indirect costs due to earthquakes in the design process. In this research, an integrated methodology for calculating life cycle cost (LCC) of moment-resisting concrete frames is presented. Increasing seismic safety of structures and reducing human casualties can play an important role in determining the optimal design. Costs incurred for structures are added to the costs of construction, including the costs of reconstruction, financial losses due to the time spent on reconstruction, interruption in building functionality, the value of people's life or disability, and content loss are a major part of the future costs. In this research, fifty years of useful life of structures from the beginning of the construction is considered as the life cycle. These costs should be considered as factors of calculating indirect costs of a structure. The results of this work represent the life cycle cost of a 4 story, 7 story, and 10 story moment-resisting concrete frame by details. This methodology is developed based on the economic conditions of Iran in 2016 and for the case of Tehran city.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.34 no.12
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• pp.77-84
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• 2018

Biomimicry architecture has been highlighted in recent years for the innovative ideas from the nature have provided main developments in many areas. The related studies in Korean architecture is entering the first step and are needed close attentions for its vitalizations. This study starts with the acknowledgement of necessity to establish the architectural application process. First of all, it is named as a 'Passive Cycle' if a problem-solving or a project has been through the analysis of process complexity and biomimicry application process in other areas. Then, the basic frame is structured as a 'Process-Cooperation,' which integrates process complexation with process circulation. The cooperative relationship starts from the beginning of the design with not only professionalists related with architecture but also ones in various areas when it is issued a difficult problem or high environmental performance not found in common architectural design processes. To make the architectural application good progress, the characteristics of bio-mimetic architectural applications are categorized as three areas; the expected effect, the application level, and application method. As a result, the organic complexity should be prioritized to have bio-mimetic application effectively while accessing the principles. Therefore, the process for the architectural application of biomimicry is suggested as 'Organic Cycle-Cooperation.'

• Architectural research
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• v.22 no.4
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• pp.105-112
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• 2020

The purpose of this study is to establish a method for assessing a building's risk against disaster, tentatively named the Korean integrated disaster evaluation simulator (K-IDES). Based on previous studies, FEMA's risk management series and FEMA IRVS are selected as case studies for developing a frame work of K-IDES, through the comparative analysis of domestic building design guides, codes, and special acts related to disasters, in order to develop a risk assessment methodology for quantitative results. The assessment method consists of a classification system and calculating risk, and a simulation applying the developed checklist in K-IDES to similar types of high-rise buildings will be conducted to validate its accuracy. The final goal is to systemize an integrated risk management in a high-rise building against disasters for the purpose of recognizing vulnerable areas from the beginning of the design process and reinforcing it from potential threats after construction.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.147.2-148
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• 2003

Complete nucleotide sequence of genome RNA of a Korean isolate of Pepper mottle virus (PepMoV-Vb) from field-collected diseased paprika (Capsicum annuum var grossum) was determined in this study. Symptoms of isolates of PepMoV were divided largely into two groups, vein banding (Vb) and vein clearing (Vc) patterns. PepMoV-Vb RNA consists of 9,640 nucleotides excluding the poly(A) tail. A single open reading frame was identified beginning at nucleotide position 169 encoding a polyprotein of 3024 amino acids which is typical of the Potyvirus genus. The complete nucleotide sequence and coding regions of PepMoV-Vb were compared to that of 11 potyviruses within the genus Potyvirus. The overall nucleotide sequence identity was 94.7 and 94.1％ identical to PepMoV-C and PepMoV-FL, respectively. Full-length cDNAs of PepMoV-Vbl were synthesized from purified viral RNAs by RT-PCR and their genome structure was analysed by RFLP analysis. This is the first report on complete nucleotide sequence of PepMoV isolated from paprika in Korea.

• Steel and Composite Structures
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• v.45 no.1
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• pp.1-21
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• 2022

In the structural analysis of steel frames, joints are generally considered as rigid or hinged considering their moment transfer ability. However, the first studies conducted with the beginning of the 20th century showed that the joints do not actually fit these two definitions. In reality, a joint behaves between these two extreme points and is called semi-rigid. Including the actual state of the joint in the structural analysis provides significant economic advantages, so the subject is an intense field of study today. However, it does not find enough application area in practice. For this reason, a large-scale literature published from the first studies on the subject to the present has been examined within the scope of the study. Three important points have been identified in order to examine a joint realistically; modelling the load-displacement relationship, performing the structural analysis and how to design. Joint modelling methods were grouped under 7 main headings as analytical, empirical, mechanical, numerical, informational, hybrid and experimental. In addition to the moment-rotation, other important external load effects like axial force, shear and torsion were considered. Various evaluations were made to expand the practical application area of semi-rigid connections by examining analysis methods and design approaches. Dynamic behaviour was also included in the study, and besides column-beam connections, other important connection types such as beam-beam, column-beam-cross, base connection were also examined in this paper.