• 한국식품과학회지
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• 제45권3호
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• pp.370-375
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• 2013

구강구취균 4종을 국내산 프로폴리스를 이용하여 프로폴리스의 농도, 열처리, pH의 변화를 통한 항균효과를 조사하였다. P. gingivalis KCTC 5352가 다소 프로폴리스에 저항적인 모습을 보였으나 그 외의 구강구취균에는 강한 항균활성을 나타내었다. 액체배지를 이용하여 프로폴리스의 농도를 조절하여 구강구치균의 민감도를 조사한 결과, 프로폴리스의 농도가 진할 수록 더 빠른 시간에 큰 활성을 보였으며 일반적으로 $0.22mg/{\mu}L$ 농도의 프로폴리스를 첨가하였을 경우 4-6시간 안에 대부분의 균이 사멸하였다. TEM을 통해 프로폴리스가 균의 세포에 어떤 영향을 미치는가 살펴보았으며 프로폴리스를 첨가한 균의 세포막이 얇아지면서 붕괴되고 안의 내부물질이 유출되면서 세포가 분해됨을 확인하였다. $0.56mg/{\mu}L$ 농도의 프로폴리스를 사용하여 열처리 후와 pH 조절 후의 프로폴리스의 항균활성을 시험한 결과 열에 안정하지 않고, pH가 높아질수록 활성이 약해지는 것으로 나타났다.

• 한국동물위생학회지
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• 제35권2호
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• pp.111-117
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• 2012

This study investigated the occurrence of honeybee diseases in Incheon area, at the point of great widespread of sacbrood disease in the country. Sixteen resident beekeeping apiaries; 3 native honeybee and 13 European honeybee apiaries were selected for this research. Over 20 adult bees were evenly collected from the most colonies of each apiary three times (March, June, November) within a year. In this work, 13 honeybee diseases including 7 viral diseases, 2 bacterial diseases, 2 fungal diseases, and 2 parasitic diseases were detected by preliminary inspections and PCR. As a result, viral infections were confirmed at 34 among 48 apiaries (70.8%) over the entire examination period. Parasitic diseases showed the highest detection rate of 45.8%, which are detected in 44 among 96 cases. In the seasonal prevalence, 30 cases (15.6%) of 7 pathogens were detected from 14 apiaries in March, 50 cases (24.0%) of 9 pathogens and 56 cases (26.9%) of 9 pathogens were detected from all apiaries in June and November, respectively. Nosema was shown to be the most prevalent pathogen from March to November, followed by sacbrood virus (SBV) and stonebrood. The spread of SBV infection in Incheon would be under-estimated by the increasing of detection rate over the time. Especially, Chinese sacbrood virus was detected from 4 European honybee apiaries, but clinical symptoms were not found. No chalkbrood, acute bee paralysis virus, and chronic bee paralysis virus were detected in this study. The effective therapy and preventive measures should be prepared for beekeeping industry.

• 복식
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• 제64권8호
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• pp.97-110
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• 2014

Animals, as a part of nature, have much influence on children's emotional development and are one of children's favorite subjects of design. This study analyzes the differences in animal expression methods and types of animals, in relation to gender and item based on a collection of children's clothing and accessories that use animal as their theme. The collection consists of 148 clothing and 75 accessories (total of 223 items), which were collected from on-line shopping sites for children's clothes. Analysis results showed the following observations. Animal expression was categorized into four methods: emblem, illustration, character, and pattern.(The most common method was character, followed by pattern, illustration, and emblem. In relation to gender and clothing item, animal expression methods varied widely:(character was the most popular for) boys' t-shirts and accessories, while girls' bottoms and dresses. As for (animal) types, Mammalia was the most common, followed by birds and insects. Dogs and puppies were popular both genders.(However, differences in preferences existed as) boys liked big and wild animals such as bears, tigers, and foxes, while girls liked small and cute animals such as rabbits, cats, owls, and butterflies. Based on these results, six types of animals (rabbits, elephants, large-beaked bird Toco Toucans, fish, penguins, and bees) were selected as themes for kids' vest designs and, among them, three designs were made.

• 농촌지도와개발
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• 제1권1호
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• pp.37-45
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• 1994

This study was conducted to investigate the organizational climate of the agricultural extension education agencies and its influence on their educators` attitude toward job in Korea. The specific objectives of the study were 1) to identify the educators` perception of the organizational climate in the agricultural extension education agencies, and 2) to determine whether or not the educators` perception of the organizational climate influnce on their attitude toward job. Organizational climate has bees defined by many scholars as the properties of the social psychological environment perceived by organization members. There are various theoretical interpretations offered by die scholas about the effect of organizational climate upon organization members` behavior. Through a series of the theoretical reviews, this study could draw more than twenty variables in four types of organizational climate which might be perceived by the educators of agricultural extension education agencies and might influence upon their attitude toward job. The data were collected mainly through questionnaires sent to the responsible officers for administering the questionnaires for all adult educators of 30 agricultural extension education agencies randomly sampled from the population of 190 agencies. After data cleaning, a total of 629 responded questionnaires were analyzed for the study. The statistical methods used in the study were percentile, correlation, one way analysis of variance and multiple regression analysis. The two major findings of the study were as follows : 1. The organizational climate of agricultural extension education agencies were classified into four types : 1) democratic-autonomous, 2) cooperative-friendly, 3) achievement-oriented, and 4) bureaucratic-authoritarian. Among these of organizational climates, the cooperative-friendly one was most positively but the bureaucratic-authoritarian one was least positively perceived by the educators. 2. The educators` potion of the organizational climate and their attitude toward job were significantly correlated. The educators` perception of the organizational climate explained 41 percent of the variance of their attitude toward job in a multiple regression analysis In particular, the perception of the achievement oriented type of the organizational climates alone explained 34 percent of the variance of the favorable attitude.

• 한국초등과학교육학회지:초등과학교육
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• 제24권2호
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• pp.130-137
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• 2005

Elementary preservice teachers' understanding about the insect molt and metamorphosis was examined. Data were collected through the paper-pencil test of 448 junior students at a National University of Education. The instrument used was developed by Son(2003) and modified for the test. The results are as follows: First, Only 3.1$\%$ of students had a scientific concept on the molt periods in the insect life cycle. Students had an alternative conception that molt occurs either only in the larval period (29.9$\%$) or only in the period from pupa to adult (22.3$\%$). The percentage of the students thinking that molt occurs in the period from larva to pupa was low. Second, 98$\%$ of students stated that molt has to do with the larval growth, but 39.1$\%$ of students had an alternative conception that molt occurs after the larval growth at each instar. 25.1$\%$ had a conception that molt is related with the larval growth, but did not show any further understanding. Third, most students understood the correct meaning of metamorphosis. 34..2$\%$ of students had an alternative conception that metamorphosis occurs only in the period changing from pupa to adult, and only a few thought that it occurs in the period from larva to pupa. 24.8$\%$ of students had a scientific concept on the periods in which metamorphosis occurs. Fourth, some students understood the hatching process as molt in the sense that the egg shell is taken off (21.0$\%$), and as metamorphosis in terms of the appearance change from egg to larva (25.0$\%$). Fifth, 35.5$\%$ of students selected bees as an insect of incomplete metamorphosis, and 35.3$\%$ responded 'I have no idea', showing that they had poor understanding about the insect life cycle.

• 한국환경복원기술학회지
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• 제23권4호
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• pp.85-96
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• 2020

The honeybee serves for most entomophilous flowers. They are a core species for maintaining the ecological system. Though the urban ecological system needs bees' mediation of pollination as well, we have little understanding on how the honeybee reacts to the physical environments of an urban city. This study is a basic research to enhance the potential environment for pollination in an urban area and aims to review the urban environmental variables which are highly linked to the pollination mediations by the honeybee. The study composed a Maxent model by adopting nine urban environmental variables and the locations of the Apis mellifera's appearances around 52 spots in Seoul. The variables reflect the ecology of the Apis mellifera. Of the urban environmental variables used for the model composition, six variables were found as not having meaningful correlations with the Apis mellifera's appearances and finally, building coverage, actual vegetation and land cover were selected as the appearance variables of the Apis mellifera. The AUC, the reliability indicator of the final model was 0.791 (sd=0.077). And the importance data of the variables used for the model were 55.6%, 27.9%, and 16.5% for building coverage, actual vegetation and land cover, respectively. The result of the study showed that the building coverage has the highest correlation with the appearance of the honeybee. And, as per the actual vegetation, the artificially tree planted area as well as the cultivated field and meadow in an urban area were functioning as the most important environmental conditions for the honeybee to be inhabitable. The study is expected to be utilized as the base material for the urban planning and park green area planning to enhance the potential environment for pollination in an urban area.

• Journal of Microbiology and Biotechnology
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• 제30권9호
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• pp.1305-1309
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• 2020

Insects possess biological defense systems that can effectively combat the invasion of external microorganisms and viruses, thereby supporting their survival in diverse environments. Antimicrobial peptides (AMPs) represent a fast-acting weapon against invading pathogens, including various bacterial or fungal strains. A 37-residue antimicrobial peptide, papiliocin, derived from the swallowtail butterfly Papilio xuthus larvae, showed significant antimicrobial activities against several human pathogenic bacterial and fungal strains. Jelleines, isolated as novel antibacterial peptides from the Royal Jelly (RJ) of bees, exhibit broad-spectrum protection against microbial infections. In this study, we developed a novel antimicrobial peptide, PAJE (RWKIFKKPFKISIHL-NH₂), which is a hybrid peptide prepared by combining 1-7 amino acid residues (RWKIFKK-NH₂) of papiliocin and 1-8 amino acid residues (PFKISIHL-NH₂) of Jelleine-1 to alter length, charge distribution, net charge, volume, amphipaticity, and improve bacterial membrane interactions. This novel peptide exhibited increased hydrophobicity and net positive charge for binding effectively to the negatively charged membrane. PAJE demonstrated antimicrobial activity against both gram-negative and gram-positive bacteria, with very low toxicity to eukaryotic cells and an inexpensive process of synthesis. Collectively, these findings suggest that this novel peptide possesses great potential as an antimicrobial agent.

• 식품보건융합연구
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• 제6권2호
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• pp.17-21
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• 2020

The response of honeybee (Apis mellifera L.) pollination on canola yield with reference to most suitable number of bee hive need per unit area of crops in order to meet optimum pollination needs and better economic yields by comparing number of hives and yield components an experiment was conducted at Beekeeping and Hill Fruit Pests Research, Station Rawalpindi during 2017-18 in complete randomized block design with two sets of four treatments for comparison: 1 hive acre^-1, 2 hives acre^-1, 3 hives acre^-1 and 0 hive acre^-1. The hives were kept inside the experimental area. Parameters were assessed: pollination density, pollinator's diversity, agronomic and economic yield. In case of pollination density, the cumulative mean abundance bee species revealed that at 1200 hours, Apis mellifera was the most abundant and frequent visitor with a mean population of 8.69 bees/plant followed by A. dorsata (0.72), Syrphid fly (0.2) and other pollinators. Minimum bee population was observed during 1400 hours, mainly due to the closure of flowers and partially due to high temperature (>35℃). Pollinator diversity revealed that A. mellifera was the most dominant pollinator of Brassica crop with highest abundance (71%). A. dosata ranked 2^nd (16%) followed by A. florea (6%) respectively.

• Journal of Veterinary Science
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• 제22권3호
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• pp.40.1-40.12
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• 2021

Background: The microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen. Objectives: The present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees. Methods: A procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration. Results: UR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 10⁴ spores/bee, and the stable detection level was ≥ 2.40 × 10⁵ spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 10⁴ copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min. Conclusions: UR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.

• Journal of Ecology and Environment
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• 제42권4호
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• pp.265-271
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• 2018

Background: Temperature-driven variation in pollinator assemblage and activity are important information, especially at high altitudes, where rising temperature trends exceed global levels. Temporal patterns of pollinators in a flowering season can be used as a proxy to predict the changes of high-altitude plants' mutualistic relationships. We observed a spring temperature change in one population of a high-altitude endemic species, Megaleranthis saniculifolia on Mt. Sobaeksan, and related it to pollinator assemblage and activity changes. Methods: This study was conducted at two sites, each facing different slopes (NE and NW), for two times in the spring of 2013 (early-flowering, April 27-28, vs. mid-flowering, May 7-8, 2013). We confirmed that the two sites were comparable in snowmelt regime, composition of flowering plants, and flower density, which could affect pollinator assemblage and activity. Pollinator assemblage and activity were investigated at three quadrats ($1m^2$ with 5-m distance) for each site, covering a total of 840 min observation for each site. We analyzed correlations between the temperature and visitation frequency. Results: Twelve pollinator species belonging to four orders were observed for M. saniculifolia at both sites during early- and mid-flowering times. Diptera (five species) and hymenopteran species (four species) were the most abundant pollinators. Pollinator richness increased at both sites toward the mid-flowering time [early vs. mid = 7 (NE) and 3 (NW) vs. 9 (NE) and 5 (NW)]. Compared to the early-flowering time, visitation frequency showed a fourfold increase in the mid-flowering time. With the progression of spring, major pollinators changed from flies to bees. Upon using data pooled over both sites and flowering times, hourly visitation frequency was strongly positively correlated with hourly mean air temperature. Conclusions: The spring temperature change over a relatively brief flowering period of M. saniculifolia at high altitudes can alter pollinator assemblages through pollinator dominance and visitation frequency changes. Thus, this study emphasizes information on intra- and inter-annual variations in the mutualistic relationship between pollinators and M. saniculifolia to further assess the warming impacts on M. saniculifolia's reproductive fitness.