• Korean Journal of Agricultural Science
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• v.39 no.2
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• pp.255-261
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• 2012

A bacterium AB-55, isolated from waste mushroom bed of Agaricus bisporus in Sukseong-myeon, Buyeo-gun, Chungcheongnam-do, Korea, was screened onto xylan agar congo-red plate by the xylanolysis method and was used to produce an xylanase in shaker buffle flask cultures containing oat spelt xylans. The phylogenetic analysis using 16S rRNA gene sequence data showed that the strain AB-55 had the highest homology (99.0%) with Bacillus subtilis and it was named as Bacillus subtilis AB-55. A xylanase was purified by ammonium sulfate precipitation (50~80%), gel filtration on sephacryl S-300, and ion exchange chromatography on DEAE sepharose FF. The molecular weight of the xylanase was estimated as 44 kDa by SDS-PAGE. Optimal pH and temperature for the xylanase activity was pH 7 and $50^{\circ}C$, respectively. N-terminal amino acid sequence of the enzyme was identified as Ser-Ala-Val-Lys-His-Gly-Ala-Ile-Val-Phe. The substrate specificity of the enzyme exhibited that it hydrolyzed efficiently oat spelt xylan as well as beechwood xylan, but showed no activity against Avicel and carboxymethyl clellulose (CMC). The enzyme activity was enhanced by $Fe^{2+}$ and $Mn^{2+}$ whereas was entirely inhibited by $Hg^+$.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2010.11a
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• pp.658-663
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• 2010

With petroleum reserves dwindling, interest has been increasing worldwide in Fischer-Tropsch synthesis (FT) as a method of producing synthetic liquid fuels and chemicals from coal, natural gas or biomass. In general, FT synthesis is operated through the gas phase fixed-bed reaction system. Recently, there are lots of study in supercritical fluid due to unique characteristics such as the quick diffusion of reactant gas, effective removal of reaction heat, and the in-situ extraction of high molecular weight hydrocarbon, such as wax. In this study, our major aim is to obtain a deeper insight into the effect of the type of support on the reaction performance over a supported cobalt catalyst in a fixed bed reactor.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.334-334
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• 2017

Representative particle sizes(RPS) are commonly used for particle size distribution of heterogeneous sediment such as bed material. RPS can provide not only information of mean characteristics of sediment, but also other properties like sorting, skewness, kurtosis. For sediment including sand and clay material, RPS is estimated through two steps. The first is experimental step for calculating weight of each size class, the next is interpolation step to get RPS by using the graph plot. At the second step, graph method known as direct reading of value along the interpolation line in the graph plot is commonly used. This method is often time-consuming job. In this study we developed a new program to get RPS by using MS Excel. Simple linear and semi-log interpolation are used. When compared with conventional graph method(direct reading), simple linear shows 5.31%, while semi-log 1.29% of relative difference. We developed MS Excel program for estimation of RPS automatically.

• Journal of the Korean Society of Propulsion Engineers
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• v.15 no.3
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• pp.15-21
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• 2011

With petroleum reserves dwindling, interest has been increasing worldwide in Fischer-Tropsch synthesis (FT) as a method of producing synthetic liquid fuels and chemicals from coal, natural gas or biomass. In general, FT synthesis is operated through the gas phase fixed-bed reaction system. Recently, there are lots of study in supercritical fluid due to unique characteristics such as the quick diffusion of reactant gas, effective removal of reaction heat, and the in-situ extraction of high molecular weight hydrocarbon, such as wax. In this study, our major aim is to obtain a deeper insight into the effect of the type of support on the reaction performance over a supported cobalt catalyst in a fixed bed reactor.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.866-871
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• 2011

An auxin-producing bacteria (3YN11-02) was isolated from waste mushroom bed from Agaricus bisporus of Chungnam Buyeo-Gun area. The strain 3YN11-02 was identified as a novel species belongs to Rahnella aquatica by a chemotaxanomic and phylogenetic nalysis. The isolate was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC and HPLC analysis. When the concentration of IAA was assessed by performing HPLC quantity analysis, the maximal $290mg\;L^{-1}$ of IAA detected in ether fraction extracted from the culture filtrate which was cultured in R2A broth containing 0.1% tryptophan for 24 h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. To investigate the growth promoting effect of crop, when the culture broth of R. aquatica 3YN11-02 was infected onto water culture and seed pot of mung bean, the adventitious root induction and root growth of mung bean were 2.0 times higher than control.

• Journal of Korean Society of Forest Science
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• v.73 no.1
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• pp.43-54
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• 1986

This study was carried out to investigate the influence of light intensity on the growth of Korean white pine (Pinus koraiensis) seedlings. The seedlings were grown under four different relative light intensities: 100%, 63%, 37%, and 19% of full sunlight by covering with saran screen on seed bed. The results obtained were summarized as follows; 1. The highest shoot elongation was found at 37% in relative light intensity (RLI) plot, and the best growth in root, seedling length and diameter increment appeared at 100% on RLI plot. Contribution rates of shading to growth in shoot elongation, root growth, seedling length, and diameter increment were 9.3%, 26.2%, 21.0%, and 48.7%, respectively. 2. The greatest number of one-year-old needle fascicle was found at 100% in RLI plot, whereas that of two-year-old needle fascicle appeared at 37% in RLI plot. The contribution rate of the light intensity to number of needle fascicle was 3.9% and factor of leaf age contributed to number of needle fascicle in 27.8%. The length of needles grown under different light intensities varied with needle ages. The longest length of new needles appeared at 37% in RLI plot, but old needles were not clear in influence of light intensity. The contribution rate to needle length by the light intensity was 2.5%. 3. The heaviest value of the fresh and dry weight of seedlings appeared at 100% of RLI plot then decreased with light intensity. The contribution rates to fresh and dry weight by the shading were 38% and 7.6% respectively. 4. The largest value of the dry weight of needles appeared at 100% of RLI plot and then decreased with light intensity. The contribution rates to dry weight of needles by the light intensity was 13.18%. 5. The values of T/R ratio increased with decreased light intensity and the contribution rate was 7.0%. 6. Positive correlation and linear regressions were recognized between dry weight of leaf and other factors (dry weight of shoot, root, seedling and diameter increment).

• Journal of the Korean Society for Railway
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• v.19 no.2
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• pp.135-144
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• 2016

It is essential to lighten the weight of switch girders in order to reduce their costs of manufacturing and make it easier to use them in construction. Lightening the weight of switch is also important to the Maglev 3-way switches system, however, the design variables should be considered very carefully if lightening is to be applied to the system, because these variables are vitally related to the levitation stability. Because Urban Maglev trains have a structure in which train bogie wraps around the guiderail, the adjustment of a girder's height is a possible way to reduce the weight. The safety of the application of this concept is ensured by repeated experiments in a test bed, however, due to a lack of space and budget limits, the design parametric study for the system model can substitute for actual application. The purpose of this paper is to study the design parameters that are concerned with levitation stability while a Maglev train is running on the Maglev 3-way system depending on the weight of the switch girders. In this study, switch girder weight is reduced by adjustment of girder height and girders are and modeled as a flexible body. The effect of the adjustment of girder height on the levitation stability can be analyzed by comparing the velocity of the train when it passes the switch girders, with the lateral gap, and the levitation gap which are obtained from the co-simulation of the Maglev train's dynamics model and flexible switching system. The results of this research will be used to design a Maglev switch.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.408-412
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• 2013

This study was carried out to investigate damp injury caused by tomato coir bag culture and prevent that. The tomato variety was used 'Minichal', and tomatoes were grown in greenhouse. The nutrient solution based on Yamazaki Tomato Standard Solution was irrigated from one hour after sunrise to two hour before sunset. The slits for darainage were made in three types; I, L, and bottom slit type. The coir bag of I and L type had six slits of 15cm length, that of bottom slit type had three slits of 15 cm length. The weight of coir bag in 24 hours after saturation was 14.2 kg in I type, 13.8 kg in L type, and 12.8 kg in bottom slit type, but there was not significant difference. The weight of coir bag after one day irrigation was 14.5 kg, 14.2 kg, and 13.3 kg at L, I, and bottm slit type, respectively. This means that the moisture content of coir bag during cultivation was lowest in bottom slit type. The number of adventitious root on stem was 160, 170, and 53 at I, L, and bottom slit type, respectively. The dry weight of root and root length were highest at bottom slit type, compared to other treatment. The marketable yield was highest 26.5 kg/20 plant in bottom slit type. For increasing yield and preventing damp injury, bottom slit type was most effective at U type bed coir bag culture.

• Journal of Environmental Science International
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• v.24 no.2
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• pp.189-196
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• 2015

This study was performed to identify the effect of mixed bed soil on growth of aerial parts and root zone of daughter plants for nursery field strawberry seedling raising with expanded chaff. The plant height and leaf area of daughter plants were highest or largest in the mixed soil of ERH +RH (100:0, v/v), followed by ERH+RH (75:25). The higher the mixing ratio of RH, the shorter the plant height or the smaller the leaf area. A similar tendency was observed in fresh weight. Within a root diameter of 0-0.4 mm and a root height range of 0.4-0.8 mm, root surface area and volume were statistically significantly better with treatment of ERH+RH (100:0, v/v) compared to those of roots treated with ERH+RH (75:25), ERH+RH (50:50) and ERH+RH (25:75). The growth rate of aerial parts and root zone of daughter plants were noticeably lower in two mixing ratios of 50:50 and 25:75. According to the mixing ratios of ERH+CD surface treatment, the number of roots was greatest in plants treated with ERH+CD (80:20, v/v) and ERH+CD (85:15) on August 1. However, the number of roots was highest in plants treated with ERH+CD (85:15, v/v) on August 15. Root length was longest in the plant with no treatment, and drastically shortened from ERH+CD (90:10, v/v) in both surface and mixed treatment. Although root weight showed a significant difference in ERH+CD (90:10, v/v) treatment, its increase was gradual. The rate of root growth was highest in ERH+CD (85:15). These study findings suggest that the content ratios of mixed soil ERH+RH (75:25, v/v) or below and ERH+CD (85:15) are thought to be desirable for the production of high quality seedlings.

• Journal of Bio-Environment Control
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• v.15 no.3
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• pp.225-230
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• 2006

Tomatoes were experimented in perlite bags for various irrigation control methods to elucidate the efficient method for nutrient solution management. The irrigation control methods were for 3 different types such as control by drainage level sensor (PROBE), control by integrated solar radiation (ISR), and control by time clock (Timer). The substrate weight was maintained stably in the proper range in PROBE treatment, regardless of daily solar radiations or growth stages. The bed weights in the treatments of ISR and Timer were changed largely. Growth as well as total yield was the highest in PROBE treatment. There was no difference in soluble solids (Brix %) among the treatments. Consequently, ISR control could be useful only with appropriate timer control and also calibration. Control by drainage level sensor was suggested to be the most satisfactory as irrigation management method.