• 한국전산구조공학회논문집
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• 제26권6호
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• pp.463-470
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• 2013

본 연구에서는 풍진동에 대한 현수교의 거동을 예측하기 위하여 바닥판의 비틀림강성을 고려하여 Mckenna and Tuama 모델(2001)을 개선한 2D 간단해석 방법을 제안하였다. 기존의 모델은 풍속이 증가할수록, 진동수가 낮아질수록 비정상적인 값을 나타내고, 비틀림모드의 공진현상을 묘사할 수 없었다. 이에 본 연구에서는 비틀림강성을 고려하여 풍속에 따른, 진동수에 따른 비틀림진동을 분석하였다. 해석결과 진동 초기의 수직모드는 점차 비틀림모드로 전이되며 수직모드는 안정적으로 진동하는 것을 확인하였다. 또한 비틀림강성 효과를 고려하여 해석을 수행한 결과 수직모드는 시간이 경과함에 따라 안정화되는 모습을 보이나 비틀림 진폭은 일정시간(약 200초) 이후 나타나기 시작하여 비틀림각을 지속적으로 유지하였으며 맥놀이 주기는 풍속이 증가하면서 점차 감소하였다. 비틀림 강성에 따라 서로 다른 풍하중의 풍속과 진동수에 비틀림모드의 공진현상을 나타내므로 실제 설계에는 반드시 이러한 영향이 고려되어야 할 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권2호
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• pp.181-189
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• 2011

Cryopreservation protocols induce partially irreversible damage to mammalian sperm plasma membranes. Previous studies have indicated that adding cholesterol to the plasma membrane, as cholesterol-loaded-cyclodextrins, improves cryosurvival of sperm. Therefore, the purpose of this study was to determine if treating sperm of Markhoz bucks with cholesterol-loaded-cyclodextrins (CLC) (0, 0.75, 1.5, 2.25 and 3 mg/ml diluted $240{\times}10^6$ sperm/ml) in Tris-citric acid-glucose diluents with and without egg yolk (containing 5% glycerol) would improve the post-thaw sperm quality. The motion characteristics were evaluated with a Computer Assisted System Analyzer (CASA); acrosome integrity and vitality were measured with the triple-stain technique. Samples were recovered before and after freezing by means of putting straws into $37^{\circ}C$ water for 30 sec and then parameters were assessed. The results showed that the treatments significantly affected motility, progressive motility, recovery rate, curvilinear velocity, beat cross frequency, live sperm with reacted acrosome, live sperm with unreacted acrosome, dead sperm with reacted acrosorne, and dead sperm with unreacted acrosome during freezing (p<0.05). However; no significant differences were found for average path velocity, straight line velocity, amplitude of lateral head displacement, straightness and linearity (p>0.05). The best results were observed for extender containing 2.25 mg/ml ($240{\times}10^6$ sperm/ml) CLC supplemented with 2.6% egg yolk. In conclusion, the findings of this study indicate improved Markhoz sperm viability and motility following treatment in the presence of egg yolk.

• Asian-Australasian Journal of Animal Sciences
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• 제33권9호
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• pp.1411-1420
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• 2020

Objective: The aim of study was to investigate the effects of season and single layer centrifugation (SLC) before cryopreservation on post-thaw bull sperm quality in Thailand. Methods: Semen was collected from 6 bulls (Bos indicus) in summer, rainy season and winter 2014 through 2016. Semen characteristics, sperm morphology, sperm kinematics, viability, chromatin structure and mitochondrial membrane were evaluated. Meteorological data were available from the local meteorological station; Results: Season had an effect on semen characteristics in the raw ejaculate, with higher proportions of normal spermatozoa and lower abnormalities in winter than in the other two seasons. Sperm kinematics, viability, DNA fragmentation index, and mitochondrial membrane potential were not different between seasons. Sperm samples selected by SLC had greater normal morphology and a lower proportion with bent tails than controls and higher values of progressive motility (PRO), beat cross frequency, linearity, straightness, wobble (WOB), and lower values of slow motility, velocity average path (VAP), velocity curved line, and amplitude of lateral head displacement than controls. In addition, SLC-selection had a favorable effect on PRO, VAP, and WOB that differed among seasons. Conclusion: Our results suggested that these bulls were well adapted to their location, with season having an effect on sperm morphology. Moreover, SLC could be used prior to cryopreservation, regardless of season, to enhance normal morphology and kinematics of bull sperm samples without adversely affecting other parameters of sperm quality. However, there was considerable variation among bulls in DNA fragmentation index, mitochondrial membrane potential and sperm viability. In addition, SLC had a positive effect on sperm morphology and sperm kinematics, which could be expected to influence fertility.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 춘계학술대회논문집
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• pp.6-9
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• 2008

Analyzing the characteristics of blood flow in the blood vessels is very important to diagnose the circulatory diseases. In order to investigate the hemodynamic characteristics in vivo, the measurements of blood flows inside the extraembryonic arterial and venous blood vessels of chicken embryos were carried out using an in vivo micro-PIV technique. The circulatory diseases are closely related with the formation of abnormal hemodynamic shear stress regions, thereby it is important to get blood velocity and vessel's morphological information according to the vessel configuration and the flow conditions. In this study, the flow images of RBCs in blood vessels were obtained using a high-speed CMOS camera with a spatial resolution of approximately 14.6${\mu}$m${\times}$14.6${\mu}$m in the whole circulation network of blood vessels. The blood flows in the veins and arteries show steady laminar and unsteady pulsatile flow characteristics, respectively. The mean blood flows merged (in veins) and bifurcated (in arteries) smoothly into the main blood vessel and branches, respectively, without any flow separation or secondary flow which accompanying large variation of shear stress. Vorticity was high in the inner regions for both types of vessels, where the radius of curvature varied greatly. The instantaneous flows in the arterial blood vessels showed noticeable pulsatility due to the heart beat, and the main features of the velocity waveforms, including pulsatile shape, retrograde flow, mean velocity, maximum velocity and pulsatile frequency, were significantly dependent on the pulsatile condition which dominates the arterial blood flow. In near future, these in vivo experimental results of blood flow measured in various extraembryonic blood vessels would be very useful to understand the hemodynamic characteristics of human blood flows and various blood flow researches for clinically useful hemodynamic discoveries as well.

• 셀메드
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• 제10권3호
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• pp.25.1-25.7
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• 2020

Allergic Rhinitis (AR) is an IgE (immunoglobin-E) mediated inflammatory condition of upper respiratory tract; main clinical features involve runny nose, sneezing, nasal obstruction, itching and watery eyes. AR is a global problem and has large variations in incidences, currently affects up to 20% - 40% of the population worldwide. It may not be a life-threatening disease per se but indisposition from the condition can be severe and has the potential to adversely affect the daily functioning of life. Classical yoga literature indicates that, components of yoga have been used to treat numerous inflammatory conditions including upper respiratory tract. A few yoga intervention studies reported improvement in lung capacity, Nasal air flow and symptoms of allergic rhinitis. This review examined various anti-inflammatory pathways mediated through Yoga that include downregulation of pro-inflammatory cytokines and upregulation of anti-inflammatory cytokines. The hypothalaminic-pitutary-adrenal (HPA) axis and vagal efferent stimulation has been reported to mediate anti-inflammatory effect. A significant reduction is also reported in other inflammatory biomarkers like- TNF-alpha, nuclear factor kappa B (NF-κB), plasma CRP and Cortisol level. Neti, a yogic nasal cleansing technique, reported beneficial effect on AR by direct physical cleansing of thick mucus, allergens, and inflammatory mediator from nasal mucosa resulting in improved ciliary beat frequency. We do not find any study showing effect of yoga on neurogenic inflammation. In summary, Integrated Yoga Therapy may have beneficial effect in reducing symptoms and improving quality of life for patients with allergic rhinitis. Yoga may reduce inflammation through mediating neuro-endocrino-immunological network. Future studies are needed to explore the mechanism how yoga might modulate immune inflammation cascade and neurogenic inflammation at the cellular level in relevance to allergic rhinitis; the effects of kriyas (yogic cleansing techniques) also need to be evaluated in early and late phase of AR. So the proposed model could guide future research.

• 한국재난정보학회 논문집
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• 제16권1호
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• pp.87-95
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• 2020

연구목적: 본 논문에서는 도플러 레이더를 활용하여 차량 운전자의 호흡과 심박을 추정하는 알고리즘을 제안하고 실험을 통해 연구의 가능성을 확인한다. 연구방법: 본 논문에서는 검출 신호의 peak 주파수 값과 가중치를 활용하는 weighted peak detection 기법을 제안한다. 정지 상태와 주행 상태에서의 실험을 통해 제안하는 두 알고리즘의 정확도를 분석한다. 연구결과: 제안하는 알고리즘을 통해 정지 상태에서 측정된 호흡과 심박 검출 정확도는 각각 95%, 96% 이상의 결과를 보였다. 또한 실제 주행 실험에서도 각 72%, 84% 이상의 정확도를 보여 주행 시의 활용 가능성을 확인하였다. 결론: 본 논문에서 제안하는 생세 신호 검출 기법은 차량 운전자의 호흡이나 심장 이상을 자동으로 검출함으로 운전자 본인 및 대형 교통사고를 예방하는 기술로 활용이 가능하다.

• 동아시아식생활학회지
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• 제10권6호
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• pp.548-559
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• 2000

This study was carried out through questionnaire in order to investigate the self-recognized monosodium glutamate (MSG) symptom complex in the eating out of housewives. The subjects were 503 housewives in Inchon and the results are summarized as follows. As for age, 51.9% of subjects were over 40 years old. Also 60.0% of the subjects received a high school education. As for occupation, full-time housewives accounted for 63.7%. Monthly household income of most subjects were 1 million won or more, and monthly food expenses for most subjects were over 300 thousand won or more. Also 71.0% of the subjects lived in apartments. As for frequency of eating out, 69.5% of subjects ate out I~2 times a month, 21.6% did 3~4 times a month, 4.5% did 7 times or more/month and 3.6% did 5~6 times a month. The higher education level, family income or food expense of subjects were, the more they ate out. Most subjects selected the menu of eating out according to preference of family. Also 63.6% of the subjects considered the amount of MSG in eating out. As for menus containing high amount of MSG, 55.8% of subject answered Korean dishes, 31.1% did Chinese dishes. After eating out, 25.1% of subjects perceived MSG symptom complex, such as thirst, tiredness and rapid heart beat, etc. Therefore, it may be necessary to reduce the amount of MSG in eating out and to develop safe and delicious menus of eating out using various natural foods.

• 한국광학회지
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• 제10권4호
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• pp.311-317
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• 1999

다이오드 레이저 펌핑 Nd:YAG 레이저를 평면 반일체 고리형 공진기를 이용하여 1064 nm 파장에서 단방향, 단일 모드로 발진시켰다. 실험에 사용된 반일체 고리형 공진기는 자기장 내에 놓여진 Nd:YAG 레이저 활성매질, 결정 석영판, 출력경으로 구성되어 전체적으로 광 다이오드로서 작용한다. 이에 대한 고유편광 이론 연구가 수행되었으며, 고유편광 모드에 대한 광손실이 계산되었다. 1.2 W, 809 nm 다이오드 레이저로 펌핑하여 155 mW의 단일 모드 출력을 얻었으며, 이때의 기울기 효율은 17%이었다. 동일한 두 레이저 시스템이 제작되었는데, 그 출력들의 맥놀이 주파수 스펙트럼으로부터 100 kHz 이하의 레이저 선폭을 갖는 것으로 측정되었고, 2 GHz 이상의 영역에서 연속적인 주파수 튜닝이 관측되었다.

• 농업과학연구
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• 제46권1호
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• pp.195-204
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• 2019

In this study, we examined the effect of a liposome-based extender (Optixcell) and a tris-citric egg-yolk extender (Triladyl) on the frozen-thawed spermatozoa characteristics and the calving rate. The percentages for the total motility of the frozen-thawed spermatozoa were similar in the Optixcell and Triladyl groups. However, among the motile spermatozoa with a straight line velocity (VSL) ${\geq}25{\mu}m/sec$, the curvilinear velocity (VCL, ${\mu}m/sec$), VSL (${\mu}m/sec$), average path velocity (VAP, ${\mu}m/sec$), amplitude of lateral head displacement (ALH, ${\mu}m$), beat cross frequency (BCF, Hz), and plasma membrane integrity of the frozen-thawed spermatozoa for the Optixcell group were significantly higher than those for the Triladyl group. Furthermore, the calving rate in the Optixcell group (79.0%) was higher than that of the Triladyl group (62.8%). However, the acrosomal membrane integrity of the frozen-thawed spermatozoa in the Optixcell and Triladyl groups was not significantly different. These results indicate that semen freezing with Optixcell improved the motility and plasma membrane integrity of frozen-thawed spermatozoa and the calving rate of Hanwoo cows (native Korean cattle). In conclusion, our results suggest that semen freezing with the liposome-based extender Optixcell is more efficient than with the tris-citric egg-yolk extender Triladyl for improved offspring production.

• 한국동물생명공학회지
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• 제36권2호
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• pp.82-90
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• 2021

In the present study, we examined if deep uterine artificial insemination (DUAI) can improve the pregnancy rate of artificial insemination (AI) using epididymal spermatozoa (ES) in Hanwoo cattle. The estrus cycles of 88 Hanwoo cows were synchronized, and 17 cows were artificially inseminated using the DUAI method with ES, 20 cows were artificially inseminated via the uterine body (BUAI) method with ES, and as a control, 51 cows were inseminated by using the BUAI method with ejaculated spermatozoa from 1 proven bull after frozen thawing. The pregnancy rate of the DUAI method (58.8%) was higher than that of the BUAI method (25.0%, p = 0.0498). The motility of ES was examined immediately after thawing and after 3 and 6 h of incubation. The rapid progressive sperm motility of the control group was significantly higher than that of the ES group immediately after thawing and after 3 and 6 h of incubation (p < 0.05). The straight line velocity and average path velocity of the ES group after 6 h of incubation were significantly lower than those in the control group (p < 0.05). The linearity and amplitude of lateral head of ES were lower than those at 6 h (p < 0.05). The flagellar beat cross frequency and hyperactivation of ES were lower than the control spermatozoa immediately after thawing and at 3 h (p < 0.05). These motility parameters suggested that ES had a low motility and fertilization ability compared to the control spermatozoa. After frozen-thawing and 3 h of incubation, the percentage of live spermatozoa with intact acrosomes in the ES was significantly lower than that in ejaculated spermatozoa (p < 0.05). Our findings suggested that the DUAI method can overcome the low pregnancy rate of ES, despite the low motility, viability, and fertilization ability of ES.