• 대한약침학회지
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• 제8권3호
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• pp.31-38
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• 2005

Objectives : This study was aimed at investigating liver protection mechanism of bear bile juice (Fel Ursi)by inducing liver toxicity through $CCl_4$ in mice and evaluated histological and serological findings. Methods : Experiment groups was categorized into untreated normal group, $CCl_4$ treated control group, and orally administered bear bile juice experiment group. At the termination of experiment, gross examination of the liver as well as histological findings, and Total protein, Albumin, Total bilirubin, Direct bilirubin SGOT, SGPT, and ALP contents in the serum were evaluated. Results : 1. For gross examination and histological findings, $CCI_4$ treated control group showed destroyed lobular structure, increased fibrosis, as well as hepatic cirrhosis. For the group treated with bear bile juice, the lobular structure suffered less damage, and showed lower level of fibrosis and liver cirrhosis compared to the control group. 2. For serum analysis, Total protein and Albumin were significantly increased in the bear bile juice experiment group than the control group. Total bilirubin and Direct bilirubin didn't show significant differences between the two groups. SOOT, SGPT, and ALP were significantly decreased in the normal and bear bile juice experiment groups compared to the control group. Conclusion : Taken together, bear bile juice can be effectively used for recovering the liver functions and further researches must be conducted to verify the efficacies of bear bile juice.

• 대한한의학회지
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• 제17권2호
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• pp.245-250
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• 1996

This study was conducted to investigation into production of bile juice and bile powder by Chinese moon bear. The color of bile juice and bile powder were appeared to bronze and bronze or yellowlish brown. Average production of bile juice(ml) and bile powder(g) per head and the powdered rate(%) of bile juice were 98.4ml, 20.8g and 20.4% during a surgical operation. Production of bile juice(ml) and bile powder(g) per head were appeared to 120.7ml-139.5m1 and 8.5g-10.2g during experimental period(31 days), respectively. Average daily production of bile powder(g) per head and the collectting days of bile juice was decreased; to 6.7g and 52.5 day in summer compared with another season(10.3g and 60 days) The production of bile juice and bile powder was not difference between seven times collection in 7 days and once collection in 7 days.

• 대한약침학회지
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• 제12권1호
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• pp.13-20
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• 2009

Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences of cytochrome b genes in the mitochondria. Results : The species-specific PCR amplified a DNA fragment of 214, 214, 295, and 167 bp from Fel ursi product, bear fur, cattle bile juice, and pig bile juice, respectively. The survey using the speciesspecific PCR indicated that some of commercial Fel ursi products were adulterated with cattle and pig bile juices. PCR-RFLP using the restriction endonucleases, HaeIII and HinfI enabled differentiation among Fel ursi product, cattle bile juice, and pig bile juice. Bear furs from two animals showed variations in PCR-RFLP patterns with HaeIII. Discussion : The detection methods of the species-specific PCR and PCR-RFLP could be useful in eliminating adulterated Fel ursi products from the market.