• Microbiology and Biotechnology Letters
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• v.27 no.3
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• pp.208-214
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• 1999

The condition for immobilization of the partially purified agarase from Bacillus cereus ASK202 and the properties of the immobilized enzyme have been investigated. Agarase was immobilized on various supports by entrapment method. The enzyme immobilized on Na-alginate bead showed the highest activity among those studied. The optimal reaction conditions of the immobilized agarase were obtained in 3%(w/v) Na-alginate for the matrix, bead diameter of 2.5mm, 1 unit of agarase solution and 1.0%(w/v) calcium chloride solution. The optimum pH and temperature of the immobilized agarase were pH and temperature of the immobilized agarase were pH 7.0 and 4$0^{\circ}C$, respectively. Km and Vmax values were 0.5mg/ml.min, respectively. The immobilized agarase conerted agar to agarobiose, and their total conversion ratio under the optimal condition was 89%.

• Applied Biological Chemistry
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• v.24 no.2
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• pp.149-152
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• 1981

The immobilization of Lactobacillus bulgaricus was investigated by various method, e.g. by use of polyacrylamide gel and Al-, Ca-, Fe- or Mg-alginate beads, and the most active immobilized cells were obtained by entrapment in a Ca-alginate beads. These immobilized microbial cells, when introduced into 4.5% lactose solution and whey solution showed maximum relative activity of 28% or lactose solution and 18% for whey solution as measured against the native microbial reference standard (100).

• Journal of Microbiology and Biotechnology
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• v.9 no.2
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• pp.132-139
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• 1999

A cell-entrapment technique using compressed air was applied to Bifidobacterium longum KCTC 3128 for the improvement of bifidobacteria viability. The main cell-entrapment matrix used was alginate, and viability improvement of the B. longum entrapped in alginate lattices was monitored along with the effects of other additional biopolymers. A prerequisite for acquiring consistent results was the uniformity of bead size and cell distribution which was achieved by using compressed air and mixing the cell suspension with sterilized alginate powder, respectively. The viability losses of the B. longum entrapped in alginate beads in the presence of three different substances logarithmically increased in relation to the reaction time, and proportionately decreased with an increased alginate concentration and bead diameter. The strongest improvement in B. longum viability was exhibited with a bead containing 3％ alginate and 0.15％ xanthan gum.

• Proceedings of the KWS Conference
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• 2000.10a
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• pp.216-218
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• 2000

In case of hardfaced wrapping roll, beadmark shape appear at wrapping roll surface due to irregular wear between weld bead. Irregular wear of this is caused by difference of hardness between weld bead. This study aims at investigating which matrix is good for removing of beadmark at wrapping roll surface. So, we make specimen with martensitic matrix and austenitic matrix. The hardfacing alloys were deposited 4 times on a SS41 steel plate using self-shielding flux cored arc welding method. Difference of hardness between weld bead of specimen with matrix of martensite was higher than specimen with matrix of austenite both as-welded and after heat treatment. Therefore, austenitic matrix is better than martensitic matrix for removing of beadmark of wrapping roll surface.

• Journal of Welding and Joining
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• v.28 no.6
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• pp.58-62
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• 2010

In this study, the bead-on-plate welding were conducted by using GMA welding and plasma-GMA welding on Al 5052 and, Al 6061 plates. For these processes, the maximum welding speeds for full penetration were compared, and various weld qualities such as bead appearance, cross-section, cracks and porosities were examined. Faster welding speed, cleaner bead surface and more stable drop transfer could be achieved by plasma-GMA welding, compared with GMA welding.

• Journal of Advanced Marine Engineering and Technology
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• v.18 no.3
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• pp.68-77
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• 1994

TIG welding process is applied for the active metal such as aluminum and titanium, also sometimes for overlay welding of superalloy. However the welding speed to be applied is very low because of the unstable bead formed in the region of high current and high welding speed. The present study was carried out to examine the basic phenomena of high speed TIG melt run welding by the 2% Th - W electrode(dia.3.2mm) of various tip shapes.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2001.04a
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• pp.208-213
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• 2001

Objective of this paper is to develop a new approach involving the use of an Artificial Neural Network(ANN) and multiple regression methods in the prediction of process parameters on bead height for GMA welding process. Using a series of robotic are welding, multi-pass butt welds carried out in order to verify the performance of the neural network estimator and multiple regression methods. To verify the developed system, the design parameters of the neural network estimator are selected from an estimation error analysis. The experimental results show that the proposed models can predict the bead height with reasonable accuracy and guarantee the uniform weld quality.

• Journal of Power System Engineering
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• v.14 no.4
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• pp.37-42
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• 2010

This paper deals with the sealing mechanism of the gasket component and the effects of design parameters for the exhaust manifold. The finite element model includes hot-end exhaust system and a simplified gasket model supplied by ABAQUS software. The mechanical behaviors of bead and body of a gasket are measured after several times of cyclic loads by gasket supplier. From the finite element analysis due to the cyclic thermal loads, the flange of exhaust manifold shows thermal expansion and contraction in longitudinal direction as well as convex and concave deformations with respect to the engine cylinder head. And, the contact pressures of the gasket beads suddenly changes by normal deformation of inlet flanges. Therefore, the magnitudes of contact pressures could be used to determine the sealing characteristics of the exhaust gas in the exhaust system. The distributions of contact pressures in gasket bead lines shows a good agreement with the engine test results.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2009.10a
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• pp.427-428
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• 2009

In the manufacture process of refrigerator cabinet, polyurethane(PU) liquid firstly is injected between outer plate and inner case in high temperature about $40^{\circ}C$, and PU foam is generated and solidified to the room temperature. There will be great residual stress in the PU foam, especially at the corners after the whole refrigerator is completely assembled. The stress condition will become more complicated under operating condition because of the large temperature difference between the freezing room and outer plate. And also, there are great differences of properties for plastic and steel which would cause different deformation under temperature gradient. The steel outer plate would expand compared to the PU foam or ABS material under operation condition, which induces a thermally bowing deformation in the refrigerator cabinet. The objective of this paper is to design an optimum bead structure or to use reinforcement in the refrigerator except for improving material properties in order to decrease the deformation.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1997.03a
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• pp.50-54
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• 1997

An expert drawbead model is developed to model a cranky drawbead in the finite element analysis of stamping processes. The expert model calculates the drawbead restraining forces (DBRF's) and bead-exit thinning, which are boundary conditions. DBRF's are calculated by considering bending force, unbending force, and friction force in order. Bead-exit thinning are due to the bending and tension during the deformation. The DBFR's and thinning computed form the mathematical model for the basic beads are compared with measurements and correction factors compensating for the differences are found using the multiple linear regression method. The composition beads are assumed to be a combination of basic beads so that the DBRF's and bead-exit thinning are computed to the sum of those of basic beads.