• Journal of Microbiology and Biotechnology
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• v.22 no.5
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• pp.622-627
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• 2012

Mast cells and basophils are important effector cells in immunoglobulin-E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of 6-methoxyluteolin, isolated from Chrysanthemum zawadskii, in the $Fc{\varepsilon}RI$-mediated allergic reaction. We determined that 6-methoxyluteolin inhibited anti-$Fc{\varepsilon}RI$ ${\alpha}$ chain antibody (CRA-1)-induced histamine release, as well as elevation of intracellular calcium concentration $[Ca^{2+}]_i$ in a dose-dependent manner. Moreover, the inhibitory effects of 6-methoxyluteolin on the cell surface expression and the mRNA level of the $Fc{\varepsilon}RI$ ${\alpha}$ chain were determined by flow cytometric analysis and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Therefore, these results show that 6-methoxyluteolin is a potent inhibitor of histamine release and calcium influx via down-regulation of the $Fc{\varepsilon}RI$ ${\alpha}$ chain.

• Applied Microscopy
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• v.15 no.2
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• pp.19-30
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• 1985

Morphogenesis of rat spleen was studied by light and electron microscope from the fetal stage till the newborn or adult stages. The results indicate as follows: at the 14th day of gestation rat spleen, as an early form, consists of intercellular spaces and mesenchymal cells. And at this stage the spleen is in a premature state, then it appears its adult condition in structures after the 7th postnatal day. Erythropoiesis is shown to be an active process in rat spleen beginning about the 18th day of gestation, and once established the process continues at least till the 7th postnatal day. At the 20th day of gestation, there are splenic nodules, trabeculae, venous sinus, and granular leucocytes such as neutrophils and basophils in rat spleen. Lymphocytes appeared to be well differentiated at the 7th postnatal day and were present till the adult stage. While degenerating erythrocytes are phagocytosed by macrophages. In conclusion, rat spleen started to be appeared from the 14th day of gestation and erythropoiesis in rat spleen was carried out for about 10 days between prenatal and postnatal stage. Erythrophagocytosis was accomplished by macrophages and it is suggested that the proper functions of rat spleen set off from the 7 th postnatal day when its structures are similar to the adult's.

• Korean Journal of Acupuncture
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• v.26 no.2
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• pp.53-60
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• 2009

Objectives: To investigate the effects of Hominis placenta pharmacopuncture on the blood picture and antioxidative activity in rats. Methods: Sprague-Dawley rats were divided into 3 groups; normal control (n=5), pharmacopuncture at CV12 (CV12 group, n=5), and pharmacopuncture at ST36 (ST36 group, n=5) once every other day for 4 weeks. Blood cell counting was performed and liver superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were analyzed. Results: Values of red blood cell and plasma cell volume were significantly higher in the ST36 group than the normal control. Values of hematocrit, total protein, and albumin were not significantly different among groups. White blood cell count and the percentage of neutrophils, lymphocytes, and eosinophils were not significantly different among groups. However, monocytes and basophils were significantly increased in the ST36, and CV12 groups, respectively. SOD and CAT in the CV12 and ST36 groups were significantly activated than in the normal control group, while the activity of GSH-Px showed no significant difference among groups. Conclusions: Based on the findings of this study, Hominis placenta pharmacopuncture may have positive impact on antioxidative capacity, thus activate various functions of the body.

• Restorative Dentistry and Endodontics
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• v.25 no.2
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• pp.212-218
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• 2000

The recruitment of leukocytes to a site of inflammation is dependent on a complex interplay of a number of cytokines. Monocyte chemoattractant protein-1 (MCP-1) is a potent chemoattractant for monocytes, whereas interleukin-8 (IL-8) has chemotactic activity for neutrophils, lymphocytes, and basophils. The purpose of this study was to determine the effects of several microbes found in infected root canal systems on the production of inflammatoy cytokines, interleukin 8 and monocyte chemoattractant protein-1 from human peripheral blood mononuclear cells (PBMC). Monocytes isolated from peripheral blood were stimulated by group A streptococci (GAS, ATCC 19615), Enterococcus faecalis (ATCC 29212), Streptococcus mutans (ATCC 10449), Streptococcus sanguis (clinical isolate), and Candida albicans (ATCC 90029) respectively. Each of these bacteria induced dose-dependent induction in IL-8 and MCP-1 determined by ELISA. IL-8 production by each bacteria was decreased in the range of the microbe-to-PBMC ratios of 0.1-1.0. Group A streptococci was the week inducer of MCP-1 production. These results suggest that different oral pathogens induce specific dose-dependent patterns of cytokine release. Such patterns may provide a means of control of the type of immune celles particularly with regard to inflammatory leukocyte recruitment.

• Journal of Marine Bioscience and Biotechnology
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• v.13 no.1
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• pp.48-57
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• 2021

Vibrio species are Gram-negative basophils that are ubiquitous in seawater, increasing in number as the water temperature increases. Humans are usually infected by the consumption of contaminated seawater or seafood. V. alginolyticus infection in humans is mainly associated with infections of the skin and ears, such as acute otitis media and cellulitis. In this study, the distribution of V. alginolyticus along the coast of Jeju Island, and its relationship with water temperature, salinity, DO, and pH was investigated. The antibiotic susceptibility of the bacteria isolated was also tested. In seawater, the Daejeong area had the highest detection rate, with 13 cases (21.7%), and the Hallim area showed the lowest detection rate, with eight cases (13.3%) in. In shellfish, the Daejeong area had the highest rate, with seven cases (23.3%), and the Seongsan and Hallim areas had the lowest detection rate, with four cases (13.3%). The overall detection rate was the highest in Daejeong area, with 20 cases (22.2%), and the lowest in the Hallim area, with 12 cases (13.3%). The detection rate was highest when the water temperature was highest.

• Food Science and Biotechnology
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• v.15 no.5
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• pp.694-697
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• 2006

The initiation of immunoglobulin E (IgE)-mediated allergic reactions requires binding of IgE antibody to its high-affinity receptor. Human basophilic KU812F cells express $Fc{\varepsilon}RI$ on the cell surface and act as effector cells in the allergic response. In this study, we investigated the effects of Scutellaria radix extract on the expression of the $Fc{\varepsilon}RI$ in human KU812F cells. Flow cytometric analysis showed that S. radix extract treatment caused a concentration-dependent decrease in $Fc{\varepsilon}RI$ expression on the cell surface. Furthermore, the level of $Fc{\varepsilon}RI$ ${\alpha}$, ${\beta}$, and ${\gamma}$ chain mRNA in KU812F cells was examined by RT-PCR. S. radix extract reduced total cellular $Fc{\varepsilon}RI$ $\alpha$ and ${\gamma}$ chain mRNA expression in a concentration-dependent manner. $Fc{\varepsilon}RI$-mediated histamine release was reduced from $21.75{\pm}1.34\;ng/10^6$ cells in non-treated cells to $16.46{\pm}1.98\;ng/10^6$ cells in S. radix extract treated cells. These results suggested that S. radix extract has the potential to down-regulate of FcRI expression and to inactivate basophils.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1577-1582
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• 2013

The effects of dietary supplementation of selenium (Se), iodine (I), and a combination of both on the blood haematology, serum free thyroxine (FT4) and free triiodothyronine (FT3) hormones and glutathione peroxidase enzyme (GSH-Px) activity were examined on twenty four (7 to 8 months old, $22{\pm}1.17$ kg live weight) Kacang crossbred male goats. Animals were randomly assigned to four dietary treatments (6 animals in each group). Throughout 100 d of feeding trial, the animals of control group (CON) received a basal diet, while the other three groups were offered basal diet supplemented with 0.6 mg/kg diet DM Se (SS), or 0.6 mg/kg diet DM I (PI), or a combination of both Se and I, each at 0.6 mg/kg diet DM (SSPI). The haematological attributes which are haemoglobin (Hb), red blood cell (RBC), packed cell volume (PCV), mean cell volume (MCV), white blood cells (WBC), band neutrophils (B Neut), segmented neutrophils (S Neut), lymphocytes (Lymph), monocytes (Mono), eosinophils (Eosin) and basophils (Baso) were similar among the four treatment groups, while serum levels of Se and I increased significantly (p<0.05) in the supplemented groups. The combined dietary supplementation of Se and I (SSPI) significantly increased serum FT3 in the supplemented animals. Serum GSH-Px activity increased significantly in the animals of SS and SSPI groups. It is concluded that the dietary supplementation of inorganic Se and I at a level of 0.6 mg/kg DM increased serum Se and I concentration, FT3 hormone and GSH-Px activity of Kacang crossbred male goats.

• Journal of Veterinary Clinics
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• v.28 no.4
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• pp.365-368
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• 2011

Next to performing an expert physical examination, a blood sample submitted for a complete blood count is the most basic tool available to owners or veterinary practitioners. Reference values of complete blood count were determined at 6 different ages in 114 Thoroughbred foals during the second month of life. Hematologic results were as follows: RBC 8.2-10.5 ($10^6/{\mu}l$), Hb 10.9-13.3 (g/dl), HCT 28.2-35.2 (%), MCV 30.7-35.8 (fL), MCH 11.9-13.5 (pg), MCHC 37.9-40.5 (g/dl), RDW 24.5-25.7 (%), PLT 146.3-256.4 ($10^3/{\mu}l$), MPV 6.7-8.3(fL), total WBC 8.1-12.5 ($10^3/{\mu}l$), basophils 0.0 ($10^3/{\mu}l$), eosinophils 0.0-0.3 ($10^3/{\mu}l$), neutrophils 2.4-8.6 ($10^3/{\mu}l$), lymphocytes 1.8-2.9 ($10^3/{\mu}l$) and monocytes 0.0-1.4 ($10^3/{\mu}l$). The results of this study serve as reference ranges for Thoroughbred neonatal foals populations and can be useful for health control, regular examination and pre-sale soundness examination.

• Microbiology and Biotechnology Letters
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• v.33 no.4
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• pp.255-259
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• 2005

IgE-dependent histamine-releasing factor (HRF) is found extracellularly to regulate the degranulation process of histamine in mast cells and basophils and known to play a predominant role in the pathogenesis of chronic allergic disease. HRF has been also identified in the intracellular region of the cell. Previously, we reported that HRF interacts with the 3rd cytoplasmic domain of the alpha subunit of Na,K-ATPase. To understand the molecular mechanism of the regulation of Na, K-ATPase activity by HRF, we investigated the interaction between HRF and TPI since TPI was obtained as HRF-interacting protein in HeLa cDNA library, using yeast two hybrid screening. Domain mapping study of the interaction between HRF and TPI revealed that the C-terminal region of the residue 156-249 of TPI is involved in the interaction with HRF. The interaction between HRF and TPI was confirmed by immunoprecipitation from HeLa cell extracts. Our results suggest that TPI is a HRF-binding protein and the interaction between HRF and TPI nay thus affect Na, K-ATPase activity.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.4
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• pp.325-328
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• 1995

The experiment was conducted to study the comparative pathology of hemic system among three different broiler chicken strains i.e. Hubbard (H), Lohmann (L) and Indian River (IR) suffering from experimentally induced Hydropericardium Syndrome (HPS). For this 50 chicks of each strain were inoculated with HPS inoculum at the age of 21 days and other 50 chicks of each broiler strain were kept as uninoculated control. After slaughtering each bird of both groups was subjected to pathological examination of heart, spleen and aorta and also for determining routine haematological parameters. The maximum values of Total Erythrocyte Count (TEC), heterophils and thrombocytes and the minimum values of Total Leukocyte Count (TLC) and Hemoglobin (Hb) content were found in H broiler strain. Percentage of monocytes, basophils and eosinophils also showed maximum decrease in H broiler chicken strain. Gross pathological lesions in the inoculated birds revealed that the heart showed ballooning due to distention of pericardial sac with pericardial fluid. Haemorrhages on the epicardium and flabbiness of the myocardium. Under the microscope, degenerative changes in myocardial tissue were seen. Lesions in the spleen included splenomegaly and haemorrhagic spots. Aorta showed flabbiness of the wall and disruption of endothelium. It is concluded that there is a marked difference in susceptibility of HPS among three different commercial broiler strains. The Hubbard broiler strain is more susceptible which is followed by the Indian River and Lohmann respectively.