• Mycobiology
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• v.36 no.2
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• pp.81-87
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• 2008

The optimal conditions for mycelial growth of Phellinus linteus ATCC 26710 were determined to be a log length of 20 cm, temperature of $30^{\circ}C$ and pH of 6.0. Mycelial growth was excellent on the mushroom complete medium, and was optimal when sucrose, man nose and glucose were supplied as carbon sources. Potassium nitrate and sodium nitrate as nitrogen sources supported good mycelial growth. To evaluate P. linteus mycelial colonization on logs, sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation techniques were used. Only sterilized short log inoculation produced good mycelial colonization. Initial mycelial growth and full mycelial colonization were best on 20 cm logs having 42% moisture content. The initial mycelial growth of P. linteus was accelerated over 12hr of sterilization. Basidiocarp formation was optimal using a burying method of logs after $5{\sim}6$ months, and fruiting body formation was superior in cultivation house conditions of $31{\sim}35^{\circ}C$ and in excess of 96% relative humidity.

• The Korean Journal of Mycology
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• v.25 no.2 s.81
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• pp.130-132
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• 1997

Artificial cultivation of Phellinus linteus was studied. The best yield (B.E. $9.4{\pm}1.6$) of basidiocarps including basidiospore was obtained using Quercus acutissima among the logs of Morus alba, Quercus acutissima, Alnus japonica, Castanea crenata. Optimum temperature and required periods for the basidiocarps formation were $25^{\circ}C$ and about 10 months, respectively.

• The Korean Journal of Mycology
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• v.28 no.2
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• pp.109-111
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• 2000

Composition of fatty acids (FAs) in Pleurotus ostreatus were analyzed by gas chromatography and compositional changes according to cultivar, fruitbody section and developmental stage (days after primordia formation) were investigated. Major FAs in oyster mushroom were linoleic acid, oleic acid and palmitic acid. Unsaturated FAs were higher in pileus portion than upper- or lower-stipe portion. Oleic acid content was increased with developmental days but linoleic acid content was highest at $3{\sim}4$ days after primordia formation and then decreased after that. And ratio of unsaturated FAs/saturated FAs was decreased with basidiocarp maturation.

• The Korean Journal of Mycology
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• v.28 no.1
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• pp.11-15
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• 2000

For artificial cultivation of Phellinus pini (Thore. Fr.) Ames, we conducted some study on mycelium growth and optimum condition for fruitbody formation. The optimum condition for mycelial growth was $25{\sim}30^{\circ}C$ at pH $5.0{\sim}6.0$. Optimum sawdust media were oak sawdust+willow sawdust+rice bran (4.5:4.5:1, V/V) and oak sawdust+pine sawdust+rice bran (4.5:4.5:1, V/V) and the optimum spawn incubation period was about $33{\sim}34$ days. Mycelial growth in the inner portion of oak log was 40 mm after 60 days and duration for first fruitbody primordia formation was about 110 days after inoculation.

• The Korean Journal of Mycology
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• v.28 no.1
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• pp.6-10
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• 2000

For artificial cultivation of Phellinus gilvus (Schw.) Pat we have conducted a study on cultural characteristics and condition of fruitbody formation. The optimum temperature was about $30^{\circ}C$ at pH $6.0{\sim}7.0$ for mycelial growth. Optimum sawdust media were oak sawdust+willow sawdust(5:5, V/V), oak sawdust+willow sawdust+rice bran (4.5:4.5:1, V/V) and oak sawdust+pine sawdust+rice bran(4.5:4.5:1, V/V) and, the spawn incubation period was about $25{\sim}26$ days. Mycelial growth in the inner portion of oak log was 200 mm after 60 days and duration for the first fruitbody primordia were formed about 90 days after inoculation.

• Journal of Sericultural and Entomological Science
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• v.44 no.2
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• pp.74-81
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• 2002

The optimal conditions for mycelial growth of P. linteus ASI 26011 were 25-30$^{\circ}C$ and pH 6.0, respectively. The mycelial growth of P. linteus was excellent on MCM medium. In case of carbon sources, the mycelial growth of P. linteus was best on the culture media that were contained with sucrose, mannose and glucose. Potassium nitrate and sodium nitrate were good for the mycelial growth of P. linteus as a nitrogen source. For comparison of the mycelial colonization of P. linteus on logs, several techniques of inoculation were tested; the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich. The initial mycelial growth and the full mycelial colonization of P. linteus were the best on 20 cm logs under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after 5-6 months of incubation was the best for formation of basidiocarp of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at the 31-35$^{\circ}C$ and over 96% of relative humidity.

• The Korean Journal of Mycology
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• v.16 no.4
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• pp.230-234
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• 1988

Ganoderma lucidum, a divine mushroom, beared the nonbasidiocarpus basidiospore without the normal basidiocarp. The fungus formed the basidial stroma from the aerial mycelium on agar media, and the basidium was developed from apical cells of the mycelium on the surface of the basidial stroma. One to four basidiospores were observed on a basidium. The nonbasidiocarpous basidiospores formed on the basidial stroma were similar to the normal basidiospores in morphology, although the nonbasidiocarpus basidiospores were slightly smaller in size. However, the number of hollow on the spore surface was remarkably smaller than that of the normal spore. The formation of nonbasidiocarpous basidiosproes was influenced by the genetic factor and the environmental factors such as light, temperature and ventilation.