• Asian-Australasian Journal of Animal Sciences
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• v.23 no.4
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• pp.430-436
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• 2010

The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat color and to determine the effect of alpha melanocyte stimulating hormone treatment on mRNA expression for the melanocortin 1 receptor, a key gene involved in coat color regulation in other species. Skin punch biopsies were harvested from the dorsal region of 1-3 yr old alpacas and three different enzyme digestion methods were evaluated for effects on yield of viable cells and attachment in vitro. Greatest cell yields and attachment were obtained following dispersion with dispase II relative to trypsin and trypsin-EDTA treatment. Culture of cells in medium supplemented with basic fibroblast growth factor, bovine pituitary extract, hydrocortisone, insulin, 12-O-tetradecanolphorbol-13-acetate and cholera toxin yielded highly pure populations of melanocytes by passage 3 as confirmed by detection of tyrosinase activity and immunocytochemical localization of melanocyte markers including tyrosinase, S-100 and micropthalmia-associated transcription factor. Abundance of mRNA for tyrosinase, a key enzyme in melanocyte pigment production, was maintained through 10 passages showing preservation of melanocyte phenotypic characteristics with extended culture. To determine hormonal responsiveness of cultured melanocytes and investigate regulation of melanocortin 1 receptor expression, cultured melanocytes were treated with increasing concentrations of ${\alpha}$-melanocyte stimulating hormone. Treatment with ${\alpha}$-melanocyte stimulating hormone increased melanocortin receptor 1 mRNA in a dose dependent fashion. The results demonstrated culture of pure populations of alpaca melanocytes to 10 passages and illustrate the potential utility of such cells for studies of intrinsic and extrinsic regulation of genes controlling pigmentation and coat color in fiber-producing species.

• Food Science of Animal Resources
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• v.44 no.5
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• pp.1142-1155
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• 2024

This study investigated the impact of packaging methods coupled with high barrier packaging loaded with titanium dioxide (TiO₂) on the quality of chilled pork. The experiment consisted of three treatment groups: air packaging (AP), vacuum packaging (VP), and vacuum antibacterial packaging (VAP). Changes in total viable count (TVC), pH value, total volatile basic nitrogen (TVB-N) value, sensory attributes, and water holding capacity of pork were analyzed at 0, 3, 6, 9, and 12 d. TVC of the VAP group was 5.85 Log CFU/g at 12 d, which was lower than that of AP (6.95 Log CFU/g) and VP (5.93 Log CFU/g). The antibacterial film incorporating TiO2 effectively inhibited microorganism growth. The VAP group exhibited the lowest pH value and TVB-N value among all the treatment groups at this time. The findings demonstrated that the application of VAP effectively preserved the sensory attributes of pork, the hardness, cohesiveness and adhesiveness of pork in VAP group were significantly superior than those in AP group (p<0.05), but not significantly compared with VP group. On the 12 d, the CIE a^* value of pork in VAP group was significantly higher (p<0.05). This exhibited that VAP could effectively maintain the freshness of chilled pork and extend the shelf life for 3 d compared to the AP group. These findings provide empirical evidence to support the practical implementation of TiO₂-loaded packaging film in the food industry.

• Korean Journal of Medicinal Crop Science
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• v.17 no.3
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• pp.165-172
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• 2009

The bramble cultivated widely in South Korea, which is known as Bokbunja-ddal-gi, is regarded as having originated from Korean native Rubus coreanus. This study was carried out to obtain basic phylogenetic information on Korean cultivated bramble (KCB) by comparing the internal transcribed spacer (ITS) regions with those of R. coreanus, blackberry (R. lanciniatus), black (R. occidentalis) and red (R. idaeus) raspberry. Sequences of the ITS 1 suggest that some KCB accessions share a significant similarity with both R. occidentalis and R. coreanus in the ITS 1 region. The ITS 2 sequences of the three KCB accessions clustered more closely to those of two R. occidentalis accessions than to those of R. coreanus. These results suggest that there exist variations in the sequences of ITS among KCB accessions and KCB accessions are more closely related to black raspberry than R. coreanus in the ITS regions.

• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.120-127
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• 2018

The rural labor force has gradually been decreasing due to the decrement of the farm population and the increment of the aging population. To solve these problems, it is necessary to develop and study autonomous agricultural machinery. Therefore, analyzing the dynamic behavior of vehicles in an autonomous agricultural environment is important. Until now, most studies on agricultural machinery, especially on ground vehicle dynamics, have been done by field tests. However, these field test methods are time consuming and costly with seasonal restrictions. A research method that can replace existing field test methods by using simulations is needed. In this study, we did basic research analyzing the effect of the travelling speed of a tractor on tire slip using simulation software. A tractor simulation model was developed based on field conditions following a straight path. The simulation was done for three ranges of speed: 20 - 30 km/h (considered the normal travelling speed range), 6 - 8 km/h (considered the plow tillage speed range) and 2 - 4 km/h (considered the rotary tillage speed range). The results of the simulation show that the slip ratio and slip angle values tended to increase as the traveling speed range of the tractor decreased. From the simulation results, it can be concluded that at low tractor speeds, it becomes more difficult to control the vehicle path. In future research, simulations will be done with various work environments such as a curved path as well as with various friction coefficient conditions, and the simulation results will be experimentally verified by applying them to an agricultural tractor.

• Current Research on Agriculture and Life Sciences
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• v.17
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• pp.79-83
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• 1999

The objective of this research was to characterize viscosity of sea tangle extract isolated from sea tangles in Korea to obtain basic data for production of dietary fiber materials with new functional properties. The viscosity of sea tangle extract was increased as the the extraction time increased. However, these values increased significantly up to 1 hour of extraction time and then slow increased in case of hot water extraction and enzymatic hydrolyzed sea tangle extract. As for addition of sugar and salts, the values of viscosity of sea tangle extract was very low in the addition of EDTA-2Na regardless of concentration. But in case of sucrose and NaCl, the viscosity of sea tangle extract were tended to decreased up to 2.5% concentration.

• Journal of Biosystems Engineering
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• v.38 no.4
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• pp.287-294
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• 2013

Purpose: Greenhouses for a protected horticulture covered with a plastic or glass are easy to have weakness in a heat loss by deterioration, damage, poor construction, and so on. To grasp the vulnerable points of heat loss of the greenhouses is important for heating energy saving. In this study, an on-site heat loss audit and energy consulting system were developed for an efficient energy usage of a greenhouse. Method: Developed system was mounted with infrared thermal and visual cameras to grasp the heat loss from the greenhouse quickly and exactly, and a trial calculation program of heating load of greenhouse to provide farmers with the information of heating energy usage. Results: Developed system could print out the reports about the locations and causes of the heat losses and improvement methods made up by an operator. The mounted trial calculation program could print out the information of the period heating load and fuel cost according to the conditions of greenhouse and cultivation. The program also mounted the databases of the information on the 13 horticultural energy saving technologies developed by the Korea Rural Development Administration and simple economic analysis sub-program to predict the payback period of the technologies. Conclusion: The developed system was expected to be used as the basic equipment for an instructors of district Agricultural Technology and Extension Centers to conduct the energy consulting service for the farmers within the jurisdiction.

• Journal of Korean Society of Rural Planning
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• v.7 no.1 s.13
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• pp.49-59
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• 2001

This study aimed at developing the through processing system of questionnaire works for public participating village appraisals. With the data-base system of standardized questions menu pool developed in the previous study, the system was developed using Visual Basic 6.0 and Access 97 and consisted of 3 subsystems; Questionnaire Design, Data-in, Analysis/Presentation. Using the system developed in this study, questionnaire works for village appraisal was carried out at the 3 sample villages and processed. The questionnaire results were compared with resources evaluation results from another past study at the same sample villages, which showed much accordance between them.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.607-612
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• 2000

Microprecision agriculture for a fully controlled plant factory is proposed in this paper. Microprecision agriculture can be attained by using plant factories to realize profitable alternative agriculture. A closed, fully controlled, plant-growing factory is far better in terms of minimizing all sorts of waste. The limit and optimum design concept has to be applied to establish an economically feasible, fully controlled, plant-growing factory. To achieve this objective, microprecision technologies have to be developed. Microprecision technologies should be involved in sensing, modeling, controlling, and collecting information for the mechatronics for plant production. Basic technologies for microprecision are already available; they are SPA (speaking plant approach to environmental control), AI (artificial intelligence: expert systems, neural networks, genetic algorithms, photosynthetic algorithms etc.), bioinstrumentation, non-invasive measurement, biomechatronics, and biorobotics. A microprecision irrigation system for plug production is an example of a microprecision technology that has actually been implemented in a plug seedling production factory.

• Journal of Korean Society of Rural Planning
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• v.9 no.1 s.18
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• pp.77-83
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• 2003

This study was carried out to provide the basic information for the water quality management of the Sumjin River Basin. The Chooryeongchon stream watershed was selected and the parameters representing water quality were investigated from May 1999 to September 2002, periodically. Yearly mean runoff ratio to the rainfall amount of the watershed was analysed as $26.6{\sim}58.8%$. Temporal variation of water quality constituents such as water temperature, pH, EC, total nitrogen, total phosphorus were analysed. The result showed that pH ranged $5.7{\sim}7.7$, EC $54{\sim}167\;{\mu}S/cm$, COD $0.8{\sim}18.1\;mg/L$, respectively. Total-N and total-P concentration ranged from 0.89 to 5.19 mg/L and from 0.0004 to 0.030 mg/L, respectively. The relationships between runoff and mass load were derived and showed high linear relationships.

• Journal of Plant Biotechnology
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• v.40 no.3
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• pp.111-124
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• 2013

Research on the basic interaction of radiation with biological systems has contributed to human society through various applications in pharmaceutical, medicine, agriculture and other technological developments. In the agricultural sciences and food technology sectors, the last few decades have witnessed a large number of pertinent works regarding the utilization of radiation for evolution of superior varieties of agricultural crops of economic importance. This review presents general information about the effect of radiation on plant specificity, dose response, and benefits. There has been summarized of the effects observed after exposure and influenced by several factors including plant characteristics and radiation features. We also report on the effect of ${\gamma}$-irradiations on plants, focusing on metabolic alterations, modifications of growth and development and changes in biochemical pathways.