• Title/Summary/Keyword: base acceleration

Search Result 328, Processing Time 0.024 seconds

Enzymatic characterization and Expression of 1-aminocycloprophane-1-carboxlyate deaminase from the rhizobacterium Pseudomonas flourescens

  • Lee, Gun-Woong;Ju, Jae-Eun;Kim, Hae-Min;Lee, Si-Nae;Chae, Jong-Chan;Lee, Yong-Hoon;Oh, Byung-Taek;Soh, Byoung-Yul
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2010.05a
    • /
    • pp.17-17
    • /
    • 2010
  • Ethylene, known as a stress hormone regulate wide developmental processes including germination, root hair initiation, root and shoot primordial formation and elongation, leaf and flower senescence and abscission, fruit ripening. The acceleration of ethylene biosynthesis in plant associated with environmental and biological stresses. 1-Aminocycloprophane-1-carboxlyate deaminase(ACCD) is an enzyme that cleaves ACC into and ammonia, a precursor of the plant hormone ethylene. Plant growth-promoting rhizobacteria (PGPR) having ACCD can decrease endogenous ACC level of tissue, resulting in reduced production of ethylene in plants. ACC deaminse was a key enzyme for protect stressed plants from injurious effects of ethylene. ACCD gene was encoded from Pseudomonas flourescens, PGPR and was cloned in Escherichia coli. We expressed the recombinant ACCD(rACCD) containing 357 amino acids with molecular weight 39 kDa that revealed by SDS-PAGE and western blot. The rACCD was purified by Ni-NTA purification system. The active form of rACCD having enzyme activity converted ACC to a-ketobutyrate. The optimal pH for ACC deaminase activity was pH 8.5, but no activity below pH 7.0 and a less severe tapering activity at base condition resulting in loss of activity at over pH 11. The optimal temperature of the enzyme was $30^{\circ}$ and a slightly less severe tapering activity at 15 - 30$^{\circ}$, but no activity over $35^{\circ}$. P. flourescens ACC deaminase has a highly conserved residue that plays in allowing substrate accessibility to the active sites. The enzymatic properties of this rACCD will provide an important reference for analysis of newly isolated ACCD and identification of newly isolated PGPR containing ACCD.

  • PDF

Dynamic Behaviour of Masonry inFilled Reinforced Concrete Frames with Non-Seismic Details (진동대실험을 통한 비내진상세를 가지는 RC 골조의 조적채움벽 유무에 따른 동적 거동 평가)

  • Baek, Eun-Rim;Kim, Kyung-Min;Cheon, Ju-Hyun;Oh, Sang-Hoon;Lee, Sang-Ho
    • Journal of the Korea institute for structural maintenance and inspection
    • /
    • v.21 no.3
    • /
    • pp.121-129
    • /
    • 2017
  • In this paper, the shake table test for the masonry infilled reinforced concrete frame with non-seismic details was carried out in order to evaluate its dynamic behaviour and damage under seismic condition. The tested specimens were the RC frame and the masonry infilled RC frame and the dynamic characteristics, such as a resonant period, acceleration response, displacement response and base shear force response, were compared between them. As a result of the shake table test, RC frame specimen had flexural cracks at the top and bottom of the column and shear cracks at the joints. In the case of masonry infilled RC frame, the damage of the frame was relatively minor but the sliding cracks and diagonal shear cracks on the masonry wall were severe at the final excitation. The resonant period of infilled RC frame specimen was shorter than that of the RC frame specimen because the masonry infill contributed to increase the stiffness. The maximum displacement response of the infilled RC frame specimen was decreased by about 20% than the RC frame specimen. It was analyzed that the masonry infill wall applied in this study contributed to increase the lateral strength of the RC frame with non - seismic detail by about 2.2 times and the stiffness by about 1.6 times.

Rice Cell Origin Recombinant Human Granulocyte Macrophage Colony-Stimulating Factor (rrhGM-CSF) Could Improve the Wound Healing in Diabetic Hamster (당뇨가 유발된 햄스터 창상치유에 미치는 벼세포 유래 GM-CSF의 효과)

  • Han, Kyu-Boem;Heo, Si-Hyun;Jeong, Jin-Ju;Han, Man-Deuk;Kim, Wan-Jong;Shin, Kil-Sang
    • Applied Microscopy
    • /
    • v.39 no.3
    • /
    • pp.253-260
    • /
    • 2009
  • GM-CSF is a multipotent growth factor, which also plays an important role during the process of wound healing. rrhGM-CSF was specifically produced from rice cell culture in our laboratory (Hanson Biotech Co., Ltd, Daejeon). The rrhGMCSF contains more oligosaccharide side chains than any other types of GM-CSF. This work was taken to evaluate the influence on wound healing of rrhGM-CSF in male golden hamsters. Full thickness skin defects of 9 mm in diameter were made in the back of hamsters, and 100 ${\mu}L$ ointment containing rrhGM-CSF 50 ${\mu}g/mL$ was applied. Control groups were given ointment without rrhGM-CSF. The wound sizes were relatively reduced and skin was well regenerated in the experimental group compared with the control group. Structurally, reepithelialization and architecture of the skin following injury were well accomplished in the experimental group. And also, positive reaction of PCNA of the skin following injury was more prominent in rrhGM-CSF containing ointment treatment group. Since this type of GM-CSF has highly glycosylated side chains, the effectiveness might be retain longer and stable, regarding acceleration of wound healing in the animal model. The present study has important implications for further development of the therapeutic manipulation of wound healing using rrhGM-CSF.

Ensemble of Nested Dichotomies for Activity Recognition Using Accelerometer Data on Smartphone (Ensemble of Nested Dichotomies 기법을 이용한 스마트폰 가속도 센서 데이터 기반의 동작 인지)

  • Ha, Eu Tteum;Kim, Jeongmin;Ryu, Kwang Ryel
    • Journal of Intelligence and Information Systems
    • /
    • v.19 no.4
    • /
    • pp.123-132
    • /
    • 2013
  • As the smartphones are equipped with various sensors such as the accelerometer, GPS, gravity sensor, gyros, ambient light sensor, proximity sensor, and so on, there have been many research works on making use of these sensors to create valuable applications. Human activity recognition is one such application that is motivated by various welfare applications such as the support for the elderly, measurement of calorie consumption, analysis of lifestyles, analysis of exercise patterns, and so on. One of the challenges faced when using the smartphone sensors for activity recognition is that the number of sensors used should be minimized to save the battery power. When the number of sensors used are restricted, it is difficult to realize a highly accurate activity recognizer or a classifier because it is hard to distinguish between subtly different activities relying on only limited information. The difficulty gets especially severe when the number of different activity classes to be distinguished is very large. In this paper, we show that a fairly accurate classifier can be built that can distinguish ten different activities by using only a single sensor data, i.e., the smartphone accelerometer data. The approach that we take to dealing with this ten-class problem is to use the ensemble of nested dichotomy (END) method that transforms a multi-class problem into multiple two-class problems. END builds a committee of binary classifiers in a nested fashion using a binary tree. At the root of the binary tree, the set of all the classes are split into two subsets of classes by using a binary classifier. At a child node of the tree, a subset of classes is again split into two smaller subsets by using another binary classifier. Continuing in this way, we can obtain a binary tree where each leaf node contains a single class. This binary tree can be viewed as a nested dichotomy that can make multi-class predictions. Depending on how a set of classes are split into two subsets at each node, the final tree that we obtain can be different. Since there can be some classes that are correlated, a particular tree may perform better than the others. However, we can hardly identify the best tree without deep domain knowledge. The END method copes with this problem by building multiple dichotomy trees randomly during learning, and then combining the predictions made by each tree during classification. The END method is generally known to perform well even when the base learner is unable to model complex decision boundaries As the base classifier at each node of the dichotomy, we have used another ensemble classifier called the random forest. A random forest is built by repeatedly generating a decision tree each time with a different random subset of features using a bootstrap sample. By combining bagging with random feature subset selection, a random forest enjoys the advantage of having more diverse ensemble members than a simple bagging. As an overall result, our ensemble of nested dichotomy can actually be seen as a committee of committees of decision trees that can deal with a multi-class problem with high accuracy. The ten classes of activities that we distinguish in this paper are 'Sitting', 'Standing', 'Walking', 'Running', 'Walking Uphill', 'Walking Downhill', 'Running Uphill', 'Running Downhill', 'Falling', and 'Hobbling'. The features used for classifying these activities include not only the magnitude of acceleration vector at each time point but also the maximum, the minimum, and the standard deviation of vector magnitude within a time window of the last 2 seconds, etc. For experiments to compare the performance of END with those of other methods, the accelerometer data has been collected at every 0.1 second for 2 minutes for each activity from 5 volunteers. Among these 5,900 ($=5{\times}(60{\times}2-2)/0.1$) data collected for each activity (the data for the first 2 seconds are trashed because they do not have time window data), 4,700 have been used for training and the rest for testing. Although 'Walking Uphill' is often confused with some other similar activities, END has been found to classify all of the ten activities with a fairly high accuracy of 98.4%. On the other hand, the accuracies achieved by a decision tree, a k-nearest neighbor, and a one-versus-rest support vector machine have been observed as 97.6%, 96.5%, and 97.6%, respectively.

Foreign Entry Strategies for Korean Fishery Firms (한국수산업의 해외진출전략에 관한 연구)

  • 김회천
    • The Journal of Fisheries Business Administration
    • /
    • v.15 no.1
    • /
    • pp.131-153
    • /
    • 1984
  • Fishery resources are still abundant compared with other resources and the possibility of exploitation is probably great. The Korean fishery industry has grown remarkably since 1957, and Korea is ranked as one of the major fishery countries. Its of fishery products reached the 9th in the world and the value of exports was 5th in 1982. But recently a growth rate has slowed down, due to the enlargement of territorial seas by the declaration of the 200 mile, Exclusive Economic Zone, the tendency to develop fishery resources strate-gically in international bargaining, the change in function of the international organizations, the expansion of regulated waters, the illegal arrest of our fishing boats, the rapid rise in oil prices, and the fall in fish prices, the development of fishery resources as a symbol of nationalism, the fishing boats decreptitude, the rise of crew wages, regulations on fishing methods, fish species, fishing season, size of fish, and mesh size, fishing quotas and the demand of excessive fishing royalties. Besides the the obligation of coastal countries, employing crews of their host countries is also an example of the change in the international environment which causes the aggravation of foreign profit of fishing firms. To ameliorate the situation, our Korean fishery firms must prepare efficient plans and study systematically to internationalize themselves because such existing methods as conventional fishing entry and licence fishing entry are likely to be unable to cope with international environmental change. Thus, after the systematic analysis of the problem, some new combined alternatives might be proposed. These are some of the new schemes to support this plan showing the orientation of our national policy: 1. Most of the coastal states, to cope with rapid international environmental change and to survive in the new era of ocean order, have rationalized their higher governmental structure concerning the fishery industries. And the coastal countries which are the objectives of our expecting entry, demand excessive economic and technical aid, limit the number of fishing boats’entry and the use of our foreign fishing bases, and regulate the membership of the international fishery commissions. Especially, most of the coastal or island countries are recently independent states, which are poorer in national budget, depend largely on fishing royalties and licence entry fees as their main resources of national finance. 2. Alternatives to our entry to deep sea fishing, as internationalization strategies, are by direct foreign investment method. About 30 firms have already invested approximately US $ 8 million in 9 coastal countries. Areas of investment comprise the southern part of the Atlantic Ocean, the Moroccan sea and five other sea areas. Trawling, tuna purse seining and five other fields are covered by the investment. Joint-venture is the most prominent method of this direct investment. If we consider the number of entry firms, the host countries, the number of seas available and the size of investment, this method of cooperation is perhaps insufficient so far. Our fishery firms suffer from a weakness in international competitive ability, an insufficiency of information, of short funds, incompetency in the market, the unfriendliness of host coastal countries, the incapability of partners in joint-ventures and the political instability of the host countries. To enlarge our foreign fishing grounds, we are to actively adopt the direct investment entry method and to diversity our collaboraboration with partner countries. Consequently, besides proper fishing, we might utilize forward integration strategies, including the processing fied. a. The enterprise emigration method is likely to be successful in Argentina. It includes the development of Argentinian fishing grounds which are still not exploited in spite of abundant resources. Besides, Arentina could also be developed as a base for the exploitation of the krill resources and for further entries into collaboration with other Latin American countries. b. The co-business contract fishing method works in American territorial seas where American fishermen sell their fishery products to our factory ships at sea. This method contributes greatly to obtaining more fishing quotas and in innovation bottom fishing operation. Therefore we may apply this method to other countres to diffuse our foreign fishing entry. c. The new fishing ground development method was begun in 1957 by tuna long-line experimental fishing in the Indian Ocean. It has five fields, trawling, skipjack pole fishing and shrimp trawling, and so on. Recently, Korean fisheries were successful in the development of the Antarctic Ocean krill and tuna purse seining. 3. The acceleration of the internationalization of deep sea fishing; a. Intense information exchange activities and commission participation are likely to be continues as our contributions to the international fishery organizations. We should try to enter international fishery commissions in which we are not so far participating. And we have to reform adequately to meet the changes of the function of the international commissions. With our partner countries, we ought to conclude bilateral fishery agreements, thus enlarging our collaboration. b. Our government should offer economic and technical aids to host countries to facilitate our firms’fishery entry and activities. c. To accelerate technical innovation, our fishery firms must invest greater amount in technical innovation, at the same time be more discriminatory in importing exogeneous fishery technologies. As for fishing methods; expanded use of multi-purpose fishing boats and introduction of automation should be encuraged to prevent seasonal fluctuations in fishery outputs. d. The government should increases financial and tax aid to Korean firms in order to elevate already weak financial structure of Korean fishery firms. e. Finally, the government ought to revise foreign exchange regulations being applied to deep sea fishery firms. Furthermore, dutes levied on foreign purchaed equipments and supplies used by our deep sea fishing boats thould be reduced or exempted. when the fish caught by Korean partner of joint-venture firms is sold at the home port, pusan, import duty should be exempted.

  • PDF

A STUDY ON THE EFFECT OF CHINCAP IN JUVENILE SKELETAL GLASS III MALOCCLUSION (유년기 III급 부정교합자에서 이모장치의 치료 효과에 관한 연구)

  • Nahm, Dong-Seok;Suhr, Chung-Hoon;Yang, Won-Sik;Chang, Young-Il
    • The korean journal of orthodontics
    • /
    • v.28 no.4 s.69
    • /
    • pp.517-531
    • /
    • 1998
  • The purpose of this study was to investigate the changes in the craniofacial skeleton subsequent to chincap therapy in the juvenile skeletal Class III malocclusion with more appropriate control samples. The experimental group consisted of 29 Korean children(14 males, 15 females) who had skeletal Class III malocclusion with prognathic mandible and were undergone chincap thorny from the beginning of treatment. The control group was composed of 21 Korean children(10 males, 11 females) who had no orthodontic treatment, but with similar skeletal discrepancies to experimental group. Lateral cephalometric radiographs at the age of 7, and 2 years later were analyized and compared with student's t-test(p<0.05). The results of this study were as follows; 1. The control group without chincap therapy had not shown any improvement of the skeletal discrepancies, but had grown to be much severe. This means that the untreated Class III patient with prognathic mandible would not be corrected by growth. For the experimental group with chincap therapy, the anterior-posterior skeletal discrepancies and mandibular prognathism were both improved. 2. Neither significant restraint nor acceleration of growth was found in the cranial base and maxilla by chincap treatment. 3. The inhibition of mandibular growth could not be accepted, but the changes of the direction of growth and morphological changes were found. 4. Vertical growth tendency was increased with chincap therapy. 5. When Putting together the results of the analyses , it seems to be the rotation and displacement of the mandible that the major treatment effects of chincap we. The changes of the direction of growth and the morphological changes also seems to contibute to the treatment effect partly. In summary, the chincap doesn't restrain the mandibular growth. But, it is considered as a useful treatment modality for correction of skeletal discrepancies and reduction of mandibular prognathism in growing Class III patients with madibular prognathism.

  • PDF

A Study on Balanced -type Oseillating Mole-Drainer(III)-Model Test for Draft Force, Torque, Power and Moment (평행식 진동탄환 암거 천공기의 연구(III)-견인력, 토크, 동력 및 모멘크에 관한 모형시험-)

  • 김용환
    • Journal of Biosystems Engineering
    • /
    • v.1 no.1
    • /
    • pp.1-6
    • /
    • 1976
  • This paper is the third one of the study on balanced type oscillating mole-drainer, the first one was presented in No 9. Gyeongsang College Report and the second one in Vol. 17, No.4 of the KSAE. In the first part of this study, the characteristics of traction forces between the nonoscillating earth working equipments and oscillating ones was compared. A model of the balanced type oscillating mole-drainer, which composed of a mechanism that may reduce the machine vibration, was designed following the dimensional analysis and similitude technique. The model test was carried out to clarify the balancing mechanism of the oscillating parts and other parts of the machine. In the light of the results from the model tests, a prototype machine was made for experimental purpose. Results from the field test by a reported in the near future. In the second report, the model tests were carried out under the same soil conditions, i.e, . oscillating frequency, running velocity, and oscillating amplitude, etc. It was clear that use of balanced type oscillating model could substantially reduce the vibration of the whole system of the machine, when compared with the nonoscillating type model. In this paper(the third report), results of investigation on the traction force, power requirement, and moment. etc, is presented. Analysis of variance technique was used for analyzing the effect of the frequency, amplitude, and running velocity on the draft force, torque, power requirements, and moments. The results obtained from the model tests are as follows, 1) By practicing a balanced-type oscillating mole-drainer, it was possible to reduce the traction resistance by 55.1-61. 2 percent of traction resistance, however, was 1.75 - 1.95 times greater than the value of resistance which was induced by use of a mole-drainer with single bullet. The resistance of rear shank against soil was considered as a main causing factor of the above results. 2) As the oscillation frequency was increased, the traction resistance was decreased. Considering on the effect of oscillation the greater the amplitude, and the slower the running velocity was, the greater the reduction ratio of traction resistance was. 3) The ratio of the traction resistance of oscillating mole-drainer to that of non-oscillating one could be represented as a function of dimensionless variable (V/$Af$). The results from the tests were well agreed with the reported results from the experim ents on oscillation plow or hoe. 4) By taking a lower value of (V/$Af$), reducing the traction resistance was possible. This fact meant, however, that the efficiency of mole drain practice would be lower. 5) It was experimentally confirmed under the same condition of soil that the variable (R/$rD1^3$) could be represented as a function of a variable($V^2/gD$) when a non\ulcornerocillating mole-drainer was used. 6) When a oscillating mole-drainer was used, the variable(R/$rD_1^{3}$) could be represented as a function of two variables ($v^2/gD_1$) and (V^2/gD_1$). 7) The torque was not affected by a change of frequency. However, a relation of proportionality existed between torque and amplitude, running velocity, and ratio of bullet diameter. When a balanced type oscillating mole-drainer with two bullets was used, torque was increased by 52.8-78. 4 percent and total power requirement was also increased. 8) Total power requirement was increased linearly in accordance with the increasing frequency, 41.96 percent of total power was used for oscillating action. The magnitude of total power requirement was 1. 8-9. 4 times greater than that of a non-oscillating mechanism. In the view point of power requirement, it was not advisable to increase the frequency, amplitude, running velocity, and ratio of bullet diameter at the same time. 9) Only the positive moment occured in the rear shank. Change of the diameter of a rear bullet, could not affect the balancing against the soil resistance. It was necessary for rear bullet to have a large resistance against soil only when the rear bullet was in backward motion. 10) Within an extent of the experimental base, optimum limits for several design factors were A=0.5cm, $f$=22.5Hz, V=O. 05m/sec, and $\lambda$=1.0 By adapting these values traction resistance was reduced by 40 percent and vibration acceleration wa s reduced by 60 percent. Even though the total , power requirements for operating a balanced type oscillation mechanism was greater ~than that of non-oscillating one, using a oscillating mechanism would be more effective. Because a balanced type oscillating mechanism is used, tractive resistance will be reduced and then the lighter . tractive equipment could be used.

  • PDF

Research on the Circumstance for Agricultural Investment of Cambodia (캄보디아 농업투자 환경에 관한 연구)

  • Lee, Kyu-Seong;Bae, Dong-Jin;Kim, Seong-Nam;Kang, Young-Shin
    • Journal of the Korean Society of International Agriculture
    • /
    • v.23 no.5
    • /
    • pp.475-484
    • /
    • 2011
  • International price of cereal has been dramatically increasing for the past few years. This price hike amplified the importance of food self-sufficiency in numerous countries due to the fact that food security is directly proportional to food self-sufficiency. In this study, we conducted a survey to provide useful information of Cambodia's agricultural environment to possible Korean agricultural investors and as to highlight Cambodia as a strong candidate for the establishment of Korea's foreign base for cereal production. The survey conducted includes information regarding Cambodia's agricultural environment and investment circumstances including the political, economical and other contributing factors affecting agricultural investment in Cambodia. Seventy percent of the Cambodia's total population engage in agriculture and this comprises about 30% of the country's GDP. This statistics reflects the possibility of Cambodia's poverty alleviation which proves that agriculture in Cambodia is the driving force for the improvement of the country's economy. In addition, low labor cost, fertile land, abundant water resources, like the Tonle sap lake and the Mekong river, and unreclaimed lands are the strong points that could attract agricultural investors to Cambodia. Poor infrastructure, irrigation systems, law reforms, including social and cultural differences may be the biggest setbacks for the acceleration of Cambodia's agriculture development. However, the Cambodian government is open and willing to make adjustments for Cambodia to be both foreign and domestic agricultural investor-friendly, expecting that it will boost its country's agricultural development. Making the best out of this opportunity, the coordination of KOICA with Korean agricultural investors in building infrastructures and with the help of the KOPIA program for the transfer of agricultural technology will benefit both countries and will play an important role in Cambodia's agriculture.