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AN ELECTRON MICROSCOPIC RADIOAUTOGRAPHIC STUDY OF PROTEIN SYNTHESIS IN VITRO IN THE PALATAL MUCOSA OF THE RAT

  • KIM Hyun Joo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.17 no.1
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    • pp.51-87
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    • 1987
  • The incorporation of ³H-proline by epithelial and connective tissue elements of rat palatal mucosae was studied in order to investigate the relative levels of protein synthesis by the epithelium and underlying connective tissue cells. Following a sixty minutes incorporation of the radioactive tracer in vitro, it was found that the suprabasal cells had most grains per unit area. Furthermore, the grains were more concentrated over the cytoplasm than the nucleus. This was in contrast with the labeling of basal cells which had twice as many grains over the nucleoplasm than that over the cytoplasm. In intermediate cells; i.e., the spinous layer, the number of silver grains per unit area was decreased from that of the suprabasal cells. In areas where desmosomes were more prominent, many grains were in touch with such desmosomes. However, the labeling appeared to be reduced as soon as the cells became flattened. Moreover, the epidermal keratohyalin granules were relatively free of grains. Except for certain intercellular surfaces the keratinized cells were generally free of grains. On the connective tissue side, silver grains were primarily localized over the fibroblasts with occasional grains being found over palatal muscle cells, neural elements and so on. Most grains over collagenous fibers were found in relation to mature collagen fibrils. Thus, protein synthesis in isolated mucosae of the rat palate appeared to take place both in epithelial and connective elements. There were no apparent tissue alterations caused by the in vitro incorporation procedure utilized under conditions of this study.

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Distribution of Phytolacca americana in a coastal sand dune

  • Min, Byeong-Mee
    • Journal of Ecology and Environment
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    • v.37 no.2
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    • pp.81-90
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    • 2014
  • This study examined the main factors affecting the distribution of Phytolacca americana, an exotic plant species in Korea, in coastal sand dunes. The areas examined from June 2004 to February 2006 were in Sindusagu where was located on Sindu-ri, Wonbuk-myen, Taean-gun, Chungcheongnam-do. The vegetation, sediment properties, sizes and ages of Robinia pseudoacacia, P. americana and Pinus thnunbergii and spatial distribution of P. americana were assessed. Firstly, correlation coefficients (CC) between P. americana's root biomass and sediment properties were not significant. Secondly, of the four community types, P. americana was not in the mixed herbaceous community and its density was the highest in the P. thunbergii-R. pseudoacacia and R. pseudoacacia community The Poisson distribution analysis revealed the distribution of P. americana to be severely clumped. The root biomass of P. americana and the basal area of R. pseudoacacia were significantly correlated, but the CCs between P. thunbergii and other two species were not significant. The ages of P. americana and R. pseudoacacia in a quadrat were significantly correlated. Thirdly, P. americana's ages in a quadrat were mostly similar to each other. Therefore, the spatial distribution of P. americana was largely influenced by R. pseudoacacia but not by the sediment properties, and plants in a narrow area were concurrently germinated.

Effect of Serotonin Uptake Inhibitors on Serotonin Metabolism in the Hypothalamus of Freely Moving Rats

  • Song, Yun-Seob;Yoon, Se-Na;Jung, Dong-Sik;Yoo, Sang-Hee;Ryu, Hyong-Kyun;Kim, Hyung-Gun
    • The Korean Journal of Physiology and Pharmacology
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    • v.4 no.6
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    • pp.439-444
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    • 2000
  • Tricyclic antidepressant clomipramine or selective serotonin reuptake inhibitors (SSRIs) have been commonly used for the treatment of premature ejaculation. In the present study, we analyzed the concentrations of serotonin and 5-hydroxyindoleacetic acid (5-HIAA) in the medial preoptic area (MPOA) of the hypothalamus by awakening animal microdialysis following administration of clomipramine and various SSRIs. We then compared the serotonin metabolism and clinical effects of clomipramine and SSRIs on premature ejaculation. Basal extracellular serotonin level in the MPOA was higher than other brain regions and it was significantly increased by clomipramine and the SSRIs. The rank order of the concentration of serotonin at the MPOA was clomipramine, sertraline, paroxetine and fluoxetine and the concentrations of 5-HIAA was vice versa. The changes in serotonin concentration at the MPOA appeared closely associated with the clinical effects of these drugs on premature ejaculation. These results suggest that the serotonergic neuronal activity in the MPOA may have an selective inhibitory influence on ejaculation, and the effects of clomipramine and SSRIs on erectile function are mainly mediated by MPOA of the hypothalamus.

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A Study on the Association between Sasang Constitutions and Body Composition in Teenagers (청소년의 사상체질과 체성분과의 상관성에 관한 연구)

  • Kim, Jong-Won;Hong, Sang-Hoon
    • Journal of Sasang Constitutional Medicine
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    • v.12 no.2
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    • pp.132-142
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    • 2000
  • Purpose In Sasang Constitution Medicine, persons are divided into four types such as Taeyangin, Taeumin, Soyangin and Soeumin according to their appearance, moral nature and characteristic of constitutional symptoms. In teenager group, diagnosis of constitution based on their appearance was so difficult that we adopted new diagnostic method which measures and compares body compositions. Methods 180 middle school students who live in Pusan were studied from 20th of March, 1999 to 20th of March, 2000. After practicing Questionnaire(I), QSCC(Questionnaire of Sasang Constitution) I , QSCCII, we have measured the height and body compositions with Inbody 3.0, five times. Results 1. The results of Sasang Constitution analysis showed 91 students were classified as Taeumin(50.3%), 75 as Soyangin(41.4%), 14 as Soeumin(7.7%), and 1 as Taeyangin(0.6%). 2. The level of total body water, muscle area, BMR(Basal Metabolic Rate), BCM(Body Cell Mass), and AMC(Arm Muscle Circumference) were increased during spring and summer. That of body weight, fat body weight, fat body weight ratio, waist hip ratio, and BMI(Body Mass Index were increased during fall and winter. 3. In Taeumin and Soyangin groups, the changed capacity of total body water, muscle area and BMR were increased with statistical significance. 4. Waist hip ratio of Soeumin group was reduced more than those of Taeumin, Soyangin groups with statistical significance.

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A Study on the Plannig Methods of Urban Environmental Forest for Considering Ecological Characteristics( I )-In the case of Gaepo neighborhood Park in the Seoul City- (생태적 특성을 고려한 도시환경림 조성기법연구( I ) -서울시 개포 근린공원을 중심으로-)

  • 이경재
    • Journal of the Korean Institute of Landscape Architecture
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    • v.23 no.3
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    • pp.48-58
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    • 1995
  • This study was conducted to investigate the plant community and to propose ecological planning methods for urban environmental forest on Gaepo neighborhood park, Gangnamgu in Seoul. Thirty-six plots that the size of each plot was 10$\times$10m(100\ulcorner) were set up and the vegetation analysis was carried out. Field survey was executed Apirl, 1994. The actual vegetation was consisted of Poplulus$\times$albaglandulosa community(73.03%), Robinia pseudoacacia community(8.30%) P. $\times$ albaglandulosa-R.pseudoacacia community(1.81%) and environmental forest (15.22%) in the survey site. According to mean importance values of each plot, survey area were divided into ten forest communities. As for the analysis of plant community structure which was classified into ten forest communities, the importance values, ecological diversity, number of species, number of individuals, basal area and crown coverage of urban environmental forest were relatively lower than those of the native community. In urban environmental forest of Gaepo neighborhood park, it showed problems for the planting method. So urban environmental forest should be constructed considering ecological characteristics based on above results.

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Species Composition and Diversity in Mid-altitudinal Moist Temperate Forests of the Western Himalaya

  • Gairola, Sumeet;Sharma, C.M.;Suyal, Sarvesh;Ghildiya, S.K.
    • Journal of Forest and Environmental Science
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    • v.27 no.1
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    • pp.1-15
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    • 2011
  • The present study was undertaken in middle altitudinal (1500 to 2500 masl) moist temperate forest of Mandal-Chopta area in the Garhwal region of Uttarakhand, India. The aim of the present study was to assess the variation in species composition and diversity in different vegetation layers viz. herb, shrub and tree, at different altitudes. Shannon-Wiener diversity index ($\bar{H}$), $Nha^{-1}$, total basal cover per hectare (G), Simpson concentration of dominance, Pielou Equitability, species richness (SR), Margalef index, Menheink index of species richness and ${\beta}$-diversity were calculated to understand community composition. Tree G ranged from 84.25 to 35.08 $m^2ha^{-1}$ and total stem density varied from 990 to 1470 Nha-1. Total SR (herb, shrub and trees) among different forest types ranged between 31 and 58. Maximum G of herb and shrub layers was recorded at lower altitudes between 1500 and 1650 masl. ${\beta}$-diversity was higher in herb layers as compared to tree and shrub layers. Dominance-diversity curves were also drawn to ascertain resource apportionment among various species in different forest types. Values of species diversity, $\bar{H}$, $Nha^{-1}$ and G were higher in the study area as compared to similar forests growing in other parts of Uttarakhand Himalaya.

Community Structure of Abies nephrolepis Habitats and Characteristics of Understory Vegetation in Mt. Gyebang and Mt. Odae (계방산과 오대산 일대 분비나무 자생지 군집 구조 및 하층식생 특성)

  • Cheon, KwangIl;Byeon, Jun-Gi;Kim, Jung-In;Park, Byeong-Joo
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.25 no.5
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    • pp.59-76
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    • 2022
  • This study was conducted to investigated woody species community classification and understory vegetation characteristics of Abies nephrolepis forest in Mt. Gyebang and Mt. Odae designated as Protected Area for Forest Genetic Resource Conservation and National Park. Vascular plants were surveyed as following; 55 family, 148 species, 3 sub species, 14 variety, 2 form 167 taxa. As an analysis of the species estimator of Jackknife 1, 29 survey plots could be representative of vegetation in study site. Results of the woody species, 3 communities were divided by cluster analysis(A=Abies nephrolepis-Tilia amurensis, B=Betula ermanii-Abies nephrolepis, C=Quercus mongolica-Abies nephrolepis). Using MRPP-test, there were significant differences between the communities indicating analysis of the appropriate group classification(p<0.01). The basal area and altitude were analyzed by negative relationship for NMS ordination. By analyzing plexus diagram of woody species, Acer pseudosieboldianum is strong associated with Quercus mongolica and Rhododendron schlippenbachii. Magnolia sieboldii is strong associated with Acer pictum subsp. mono etc.

An Immunohistochemical Study of Pheasant Testis in Active, Inactive and Damaged States (번식기, 비번식기 및 손상상태에 따른 한국꿩 고환의 면역조기화학적 연구)

  • ;Karl-heinz Wrobel
    • Korean Journal of Poultry Science
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    • v.24 no.3
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    • pp.107-116
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    • 1997
  • In order to achieve optimal reproductive performance, reliable morphological and physiological basic data on the reproductive organs are desirable. Adult male Korean ring-necked pheasant in inactive(mid of January) and active state (end of April) were used in this study. In addition, five active state pheasants were received a single dose of 60Co-ray 500 rads each to damage the testes. The objective of this study was to investigate the distribution pattern of protein gene product (PGP) 9.5 and ${\alpha}$-tubulin in the pheasant testes of the active, inactive and ${\gamma}$-ray irradiated active states. The results obtained were summarized as follows 1. The seminiferous tubules collected in inactive states( mid of Jan) showed narrow lumen, and the spermatogonia and the Sertoli cell were well preserved. The PGP 9.5 immunoreactivity of these tubules showed a positive reaction in paranucleus area of the spermatogonia, and a positive reaction in a small number of the Leydig cells in the interstitium of the seminiferous tubules. 2. The seminiferous tubules were dilated in active state(end of April) as compared with the inactive state. The PGP 9.5 reactivity in these tubules showed a positive reaction in many Leydig cells in the interstitium of the seminiferous tubules, and the testes of ${\gamma}$-ray irradiated group showed partially weak reaction in the interstitium of the seminiferous tubules. 3. The ${\alpha}$-tubulin reactivity in the seminiferous tubules of the inactive testes was strongly positive in the cytoplasmic process of the Sertoli cell from the basal stem region to the apical ex-tension. From the broad part of the stem region to the luminal space, the active testes showed a strong positive reaction. The ${\gamma}$-ray irradiated groups showed diminished reaction in the basal region.

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Interleukin-2 Inhibits Secretin-Induced Bile Secretion in Cholangiocytes

  • Ko, Yoo-Seung;Hwang, Seock-Yeon;Park, Jae-Seung
    • Biomedical Science Letters
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    • v.19 no.2
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    • pp.158-163
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    • 2013
  • Cholestatic liver is associated with hepatic inflammation and elevated proinflammatory cytokines. Recent studies indicate that certain cytokines can modulate bile secretion. In the present study, we have examined the role of interleukin (IL-2) on the bile secretion by a combination of study models. To examine the relevance of IL-2 on bile secretion, the expression of IL-2 and IL-2 receptor (IL-2R) of isolated normal and bile duct ligated (BDL) rats cholangiocytes was first measured by RT-PCR. In BDL rats, the expression of IL-2 and IL-2R was significantly increased compared with normal rats. To study the effect of IL-2 on bile secretion, bile flow was measured in normal and BDL rats. At the level of cholangiocytes, secretory responses of isolated bile duct unit (IBDU)s were quantified by videomicroscopy. The administrations of IL-2 had no significant effect on basal bile secretion in normal and BDL rats. There was no significant effect of IL-2 on basal bile ductular secretion as evidenced by no significant difference in luminal area of the IBDUs perfusedwith 100 pM of IL-2 from those of albumin carrier control. However, the secretin-stimulated bile ductular secretion was significantly (P < 0.01) inhibited by $34{\pm}4%$ (normal, n = 12), $21{\pm}5.3%$ (BDL 2 wk, n = 12) and $15{\pm}5.2%$ (BDL 4 wk, n = 12) with the co-administration of IL-2. As with other cytokines, physiologically relevant concentration of IL-2 can significantly inhibit secretin-stimulated bile ductular secretion. These findings support the important roles of cytokines in modulating bile secretion and may contribute to the cholestasis seen in cholestatic liver diseases.

The Review of Parkinson's Disease(PD) for Physical Therapist (파킨슨 질환에 대한 문헌적 고찰)

  • Kim, Jin-Ung;Ghang, Goon-Yong;Bae, Soo-Chan
    • The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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    • v.8 no.2
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    • pp.73-87
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    • 2002
  • Parkinson's disease(PD) is a progressive neurodegenerative disease that affects the functioning of the basal ganglia, a brain area that contributes to the control of movement. The disease is caused by the death of nerve cells in the brain that produce dopamine, a chemical messenger. The cells affected usually produce a neurotransmitter(a chemical that transmits nerver impulses) called dopamine, which acts with acetylcholine, another neurotransmitter, to fine-tune muscle control. In Parkinson's disease, the level of dopamine relative to acetylcholine is reduced, adversely affecting muscle control. When the supply of dopamine is depleted, the function of the basal ganglia is disrupted and its ability to control movement deteriorates. The result is that PD patients experience moderate rigidity, difficulty in initiating movements and slowness in executing them, and a rhythmical tremor at rest. Although the cause of Parkinson's disease is not known, genetic factors may be involved. About 3 in 10 people with the disorder have an affected family member. About 1 in 100 people over the age of 60 in the US have Parkinson's disease. And Parkinson's disease is slightly more common in men. The course of the disease is variable, but drugs may be the best effective in treating the symptoms and improving quality of life. But, The doctor may arrange physical therapy to help with physical mobility problems. It is important to continue to exercise and take care of your general health. Try to take a walk each day. Stretching exercises can help you maintain your strength and mobility. So, This papers will serve about the information of PD for clinical physical therapist. Finally, The aim of review is increasing approach method and technique for PD patients by the view of physical therapy.

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