• Title/Summary/Keyword: barley protein

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Effect of Barley Mixtures on Forage Production and Soil Characteristics in Dry Riverbed of Ansancheon (안산천에서 청보리 혼파조합이 사초 생산성과 토양 특성에 미치는 영향)

  • Kim, Jong-Duk;Ko, Ki-Hwan;Kwon, Chan-Ho
    • Korean Journal of Organic Agriculture
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    • v.21 no.4
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    • pp.701-710
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    • 2013
  • This experiment was carried out to determine the forage production and soil characteristics in mixtures of barley and annual legumes at riverbed. The dry matter (DM) and total digestible nutrients (TDN) in barley and hairy vetch mixture were higher compared to other mixtures. Barley, single cropping was the highest in DM content. Comparing different mixtures, barley and crimson clover mixture was higher than other mixtures in DM contest. The crude protein (CP) content of barely and hairy vetch mixture was highest, but ether extract (EE) and TDN contents were lower than other mixtures. Barely and hairy vetch mixture significantly removed organic matter and phosphorus from riverbed through forage production (P<0.05). The total nitrogen (TN) of barley and hairy vetch mixture was highest because of high nitrogen fixation in riverbed soil. Single barley was highest in organic matter (OM) of soil. Comparing different mixtures, barley and crimson clover mixture was higher than other mixtures in OM contest. The available P2O5 of soil in barley and crimson clover mixture and barley and rape mixture were higher than others, and that of barley and hairy vetch was lower than other mixtures. The barely and hairy vetch mixture was highest in cation exchange capacity (CEC) content. Therefore barley and hairy vetch mixture is more suitable in forage mixtures because of high forage production and protein, and reduction of soil phosphorus in riverbed.

Effect of Nutrition Permeability from Barley sprouts, Curcuma longa L., Dendropanax morbifera LEV., Phellinus linteus Using Cryogenic Grinding Technology (동결분쇄를 이용한 보리싹, 울금, 황칠, 상황버섯의 영양성분 증진 및 투과 효과)

  • Lee, Il-nam;Han, Ye-eun;Jeong, Ho-jun;Park, Haeun;Jung, Juyeong;Rhee, Jin-Kyu
    • Food Engineering Progress
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    • v.21 no.4
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    • pp.391-402
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    • 2017
  • The purpose of this study was to improve the nutrition and the permeability of functional plants by using cryogenic grinding technology. Barley sprouts, Curcuma longa L., Dendropanax morbifera LEV., Phellinus linteus were dried, ground and extracted in different temperature conditions. Powder size of barley sprouts and Curcuma longa L. were about $50{\mu}m$ and Dendropanax morbifera LEV. and Phellinus linteus were about $20{\mu}m$. Cryogenic ground of Barley sprouts preserved 18.27-124.65% of nutrients such as protein, ash, carbohydrate, beta carotene, minerals, vitamins. Cryogenic grinding powder of Curcuma longa L. show high nutrients retention rate of lipid and carbohydrate. Permeability was measured by Parallel Artificial Membrane Permeability Assay (PAMPA) to predict passive gastrointestinal absorption. Permeability of saponarin, which is marker compound of Barley sprouts, is 9.88 times higher in cryogenic grinding powder than ambient grinding powder. Curcumin permability is 3.1 times higher than ambient grinded powder. As a result, particle size, nutrition, protein digestion degree and permeability demonstrated a positive relationship with the decreasing grinding temperature for the powders. These results confirm that the cryogenic grinding method had good suitability to increase functionality of plants, since it could minimize the heat generated while processing and effectively reduce the particle size.

Appropriate Electrophoresis Techniques and Isozymes to Identification of Barley Cultivars (보리품종 구분에 적합한 전기영동법과 효소)

  • Son, Eung-Ryong;Lee, Yong-Se;Yoon, Kyung-Eun;Ha, Yong-Woong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.4
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    • pp.405-411
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    • 1985
  • The buffer soluble proteins were extracted from six cultivars of barley grains and analyzed by various electrophoresis; 7.5% polyacrylamide slab gel, 2-30% polyacrylamide porosity gradient tube gel, isoelectric focusing (pH4-9) and starch gel electrophoresis. The proteins, esterase, acid phosphatase, malate dehydrogenase, glutamate dehydrogenase and leucine aminopeptidase were investigated to find out the best method to differentiate barley cultivars. The result were that protein and esterase bands in 2-30% polyacrylamide porosity gradient tube gel electrophoresis and protein bands in 7.5% polyacrylamide slab gel electrophoresis showed typical varietal differences. Therefore, those methods were suitable for differentiation of barley cultivars. It was difficult to differentiate the cultivars by the other methodes and patterns of the other enzymes.

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Comparisons between Micro-Kjeldahl and Near Infrared Reflectance Spectroscopy for Protein Content Analysis of Malting Barley Grain (근적외분광분석법과 Micro-Kjeldahl 법 간의 맥주보리 종실의 단백질함량 분석 비교)

  • Kim, Byung-Joo;Suh, Duck-Yong;Suh, Hyung-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.5
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    • pp.489-494
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    • 1994
  • Near Infrared Reflectance Spectroscopy(NIRS) has been used as a tool for the rapid, accurate, protein assay of malting barley. NIRS used in this study was filter type instruments, Neotec 102. The objective of this study was to obtain the best calibration equation, for the rapid, ease and accurate protein content analysis of malting barley using NIRS system. The optimum wavelength for protein content analysis used NIRS were 2095nm, 2095/1941nm, 2095/1941/2282nm, 2905/1941/2282/2086nm, respectively. Mean protein content with this calibration equation in NIRS analysis was 10.59%, while 10.60% in Micro-Kjeldahl one. The range of protein content in Micro-Kjeldahl was 8.66~12.66% and that in NIRS was 8.80~12.35%. When 18 other varieties produced in 1992 were analysed with 2095nm, 2095/1941nm, 2095/1941/2282nm, 2095/1941/2282/2086nm equation, standard deviation of difference (SDD)and standard error of performence(SEP) and $R^2$ values were 0.47, 0.43, 0.95, respectively. Both the mean protein content by Micro-Kjeldahl and by NIRS was 10.25%. With this equation, analysied 31 varities produced in 1993, SDD and SEP and r values were 0.69, 0.67, 0.91, respectively, and that bias value was 0.65. In this analysis, mean protein content by Micro-Kjeldahl was 10.17% and by NIRS was 10.81%. The range of protein content in Micro-Kjeldahl was 7.58~14.29%, What that in NIRS was 8.63~13.93%. After adjusted bias in the best calibration equation, mean protein content of Micro-Kjeldahl was 10.17% and that of NIRS was 10.09%, without variance of SDD, SEP and r values.

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Comparative of Physicochemical and Sensory Quality Characteristics of Cookies added with Barleys and Oatmeals (보리와 귀리첨가 쿠키의 이화학적 및 관능적 품질특성비교)

  • 이정애;박금순;안상희
    • Korean journal of food and cookery science
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    • v.18 no.2
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    • pp.238-246
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    • 2002
  • The purposes of this study were to provide the basic information on the cookies made with barley and oatmeal powder and to establish an optimum formula for the development of cookies with high content of fiber. Cookie samples were prepared with barley and oatmeal at various mixing ratios (2:0, 1:1, 2:1, 0:2), and the chemical properties, textural characteristics, and sensory properties of them were evaluated. The contents of carbohydrate and moisture of barley cookies were higher than oatmeal cookies, but the contents of crude protein, crude fat, crude ash and total fiber of oatmeal were higher than those of barley. The moisture content was the highest in the cookies of oatmeal and the lowest in barley-used ones. The cookie volume was increased by the addition of barley and oatmeal. The results of sensory evaluation showed that the cookies containing barley and oatmeal (2:1) was significantly more preferable in overall acceptability than the others. Hunter color test showed that the lightness was the highest in control, but the redness and yellowness were increased in the cookies with barley and oatmeal compared with the control.

Effect of Barley Bran Flour Addition on the Quality of Bread (보리등겨 가루 첨가가 식빵의 품질에 미치는 영향)

  • Choi, Ung-Kyu
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.746-750
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    • 2005
  • Effect of barley bran flour on bread quality was investigated. With addition of barley bran flour, crude protein and ash contents of bread increased, and color of crumb and crust became darker than control group made with pure wheat flour. Content of dietary fiber in bread made with 5% barley bran flour was twofold higher than control group. Hardness of breads increased and volume decreased in proportion to bran content. Sensory qualities of 5% barley bran flour-added group and control group were not significantly different, but decreased in 10 and 15% barley bran flour-added groups, revealing optimal content of barley bran flour to be 5%.

Changes in Chromatographic Fractionation and Composition of the Proteins of Malting Barley Grain during Germination (발아기간별 맥주맥 단백질의 분획 및 조성변화)

  • Seo, Ho-Soo;Cho, Sung-Hwan
    • Korean Journal of Food Science and Technology
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    • v.24 no.1
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    • pp.1-6
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    • 1992
  • Changes in protein distributiun, eletrophoretic patterns and amino acid composition were investigated during germination of malting barley. Fractionation of the protein complex in ungerminated malting barley resulted in a higher hordein fraction but less glutelin fraction of the protein complex in ungerminated malting barley resulted in a higher hordein fraction but less glutelin fraction as compared to germinated malting barley. As germination proceeded, NPN, globulin and glutelin fractions continued to increase, accmpanied by decreases in albumin and hordein fractions. The electrophoretic pattern of malting barley proteins showed three bands (molecular weight range of $15,000{\sim}41,000$ daltons) in albumin fraction, five bands ($19,000{\sim}61,000$ daltons) in globulin fraction, five bands ($18,000{\sim}56,000$ daltons) in hordein fraction and tour bands ($20,000{\sim}47,000$ daltons) in glutelin fraction, exhibiting quantitative changes in each fraction during germination. Amino acid analysis showed that glutamic acid, histidine, aspartic acid, serine, glycine, valine, alanine and leucine were major amino acids of proteins in malting barley grains. Glutamic acid increased slightly, but other amino acids showed no definite trend as germination proceeded.

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THE EFFECT OF RICE STRAW-POULTRY MANURE SILAGE AND BARLEY ON THE NITROGEN DIGESTION AND MICROBIAL PROTEIN SYNTHESIS IN THE RUMEN OF SHEEP

  • Lee, Nam-Hyung;Yoon, Chil-Surk
    • Asian-Australasian Journal of Animal Sciences
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    • v.2 no.4
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    • pp.615-623
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    • 1989
  • Three sheep fitted with rumen cannulae and abomasal cannulae were given daily 750 g (DM) of three diets consisting of straw-manure silage and barley mixture in the ratios of 75:25, 50:50 and 25:75. As the proportion of barley in the diet increased, there was an increase in the amount of OM apparently digested in the rumen and thole tract (P<.01). But ADF digestion was decreased. For the 25:75 diet the $NH_3-N$ content in the rumen showed the highest value, but the total VFA was the lowest. The rumen volume and dilution rate increased with increasing ratio of silage in diets. There were no significant differences between diets in abomasal NAN flow, and the bacterial-N for the 25:75 diet was 7.3 g N as compared with 9.2-9.6 g N for the other diets (P<.01). Rates of bacterial nitrogen synthesis in the rumen were 30.5, 24.1 and 14.9 g N per Kg OM apparently digested in the rumen for the 75:25, 50:50 and 25:75 diets, respectively.

Structural Characterization of Hordeum vulgare L. Chloroplast by Ozone

  • Chung, Hwa-Sook;Lim, Young-Jin;Park, Kang-Eun
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.4 no.2
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    • pp.85-94
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    • 2000
  • The effects of ozone on chloroplast development in barley seedlings during greening was investigated based on ultrastructural changes in the chloroplasts and band pattern changes in the chloroplast thylakoid membrane proteins. In this analysis of the chloroplast thylakoid membrane thylakoid protein band pattern by SDS-PAGE, none of the 24-hour greening bands included were clearer than the control. This means that the ozone treatment produced a dealy in chloroplast development and decreased the amount of thylakoid membrane proteins. LHC II chloroplast band of developing barley seedlings treated with 0.5 and 1.0 ppm ozone during the last 4 hours of the 24-hour greening period was weaker than the other bands. This result indicates that ozone affects the LHC II protein complex of the chloroplast thylakoid membrane. When investigating the ultastructural changes in ozone-treated chloroplast, the main site affected by 0.5 ppm ozone was the chloroplast grana, thereby explaining the delayed chloroplast development during the early phase of greening. In addition, there was also a structural change in the stromal grana of the ozone treated chloroplast during the middle phase of greening. The effects of ozone on the chloroplast of barley seedlings during the last phase of 48-hour greening were more functionally inhibiting than structural changes.

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Radial Distribution Proximate Composition and Minerals within Naked Barley Kernel (쌀보리 입자내의 일반성분 및 무기질의 분포)

  • Park, Sung-Hee;Kim, Kwan;Kim, Sung-Kon;Park, Yang-Kyun
    • Applied Biological Chemistry
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    • v.32 no.4
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    • pp.441-443
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    • 1989
  • The major kernels$(7{\sim}10\;mesh)$ of naked barley were pearled to give an average yield for each pearling of about 5% flour, with 70% of the naked barley left as residual kernel. The contents of protein, fat and ash were in the highest in the fraction of 1,2 and 3, respectively. These fractions contained 1, 4 times of protein, 3.16 times of fat and 3.08 times of ash more than those values of original kernel. Residual kernels contained 62% of protein, 38% of fat and 35% of ash in the original kernel. Among minerals, the content of Ca, Na, Fe and Mn were in the highest in fraction 1 ; P, Mg and Zn in fraction 2 ; and K and Cu in fraction 3. Concentrations of these minerals were the lowest in the residual kernel. Magnesium showed the deepest concentration gradient, while iron was evenly distributed within the kernel.

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