• Title/Summary/Keyword: bark content

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Protein Production from Cellulosic Wastes by Mixed Culture of A. phoenices and C. utilis (Aspergillus phoenicis 및 candida utilis의 혼합배양에 의한 섬유소로부터의 단백질 생산)

  • 이영녹;박경량;이주실;배광성;백대홍
    • Korean Journal of Microbiology
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    • v.19 no.1
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    • pp.14-22
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    • 1981
  • Protein content of cellulosic wastes, such as spent grain, hop bark, spent rye, rice straw, rice hull, saw dust and used newspaper, was increased by a mixed culture of C. utilis wastes having 66-75% moisture. Among the fungal strains tested. A.phoenicis KU175 was the most powerful to increase the protein content of A. phoenicis during the mixed culture with C. utilis in the CMC medium reached at the peak for one day culture after inoculation of the both strains at the same time, while it reached at peark from the beginning of the mixed culture, when A. phoenicis was inocultated for 12-24hours prior to the inoculation of C.utilis. To increase the protein content of the cellulosic wastes by the mixed culture of C.utilis and A.phoenicis, the inoculation of both strains at the same time was more effective than the preinoculation of A. phoenicis for 6-24 hours. Content of crude cellulose in the used newspaper, saw dust and spent grain was high relatively, and the lignin content of spent grain, spent rye, and rice strew was reduced more than half by the treatment of 2% NaOH. However, effect of alkali treatment of increase the protein content of the cellulosic wastes was not prominent in the case of mixed culture. Protein content of the cellulosic wastes was increased prominently by the mixed culture of C.utilis and A.phoenicis in semi-solid substrate, compared with the single culture of C. utilis, although the latter increased the protein content of cellulosic wastes considerably. The effect of mixed culture of C. utilis and A. phoenicis increased 4-fold the protein content of spent grain, and more than doubled crude protein in hop bark and rice straw.

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Analysis of Taxol and Cephalomannine in Cheju Island Yew (Taxus cuspidata S. et Z.) (제주도 주목(Taxus cuspidata S. et Z.)의 Taxol 및 Cephalomannine의 함량분석)

  • Kwak, Sang-Soo;Choi, Myung-Suk;Park, Young-Goo;Ko, Kyung-Soo;Liu, Jang-Ryol
    • Korean Journal of Pharmacognosy
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    • v.26 no.2
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    • pp.116-121
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    • 1995
  • To seek for a stable supply source of taxol as an anticancer agent urgently needed for the treatment of ovarian cancer, the concentrations of taxol and cephalomannine in bark and needles of 37 yew (Taxus cuspidata S. et Z.) trees grown in 6 locations of Cheju Island were analyzed by using HPLC. A considerable variation in the content was observed in plant part and the location. The average taxol content in the needles ($220.3{\;}{\pm}{\;}56{\;}{\mu}g$ per g dry wt) was ca. 4 times hgher than that in the bark, whereas the content of cephalomannine in the needles $(353{\;}{\pm}{\;}141{\;}{\mu}g)$ was ca. 5 times higher than that in the bark. Particularly, even within Cheju Island, needles from trees grown near the coast including Sogwipo area contained much higher taxol contents than needles from trees grown at mountain locations, indicating that taxane level is affected by environmental effects. Taxol in the needles was confirmed by electrospray mass spectrometry. These results suggest that the needles of yew trees growing in Cheju Island may provide a renewable source for taxol.

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Effects of Sterilization for Quality Control on the Content of Paeonol in Moutan Radicies Bark (품질 보증을 위한 멸균법이 목단피의 패오놀 함량에 미치는 영향)

  • Lee, Yong-Soo;Shin, Woon-Seob;Cho, So-Yean;Ze, Keum-Ryon;Lee, Hyo-Min;Jeong, Choon-Sik
    • YAKHAK HOEJI
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    • v.49 no.2
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    • pp.180-184
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    • 2005
  • The purpose of this study was to develop the best methods to sterilize Moutan Root Bark which is frequently used as a herbal medicine, and known to have high susceptibility to m icrobial contamination. We used dry heat, gamma irradiation and alcohol gas treatment for sterilization, and evaluated these methods in terms of the followings; I) the efficacy of sterilization, and ii) the chemical alteration of a major component of the harbal medicines. Treatment with dry heat effectively got rid of contaminated microorganisms, and did not significantly alter the content of paeonol. However it seriously changed the color and morphology which are an essential criterion to estimate a measure of quality of medicinal herbs. Treatment with gamma irradiation showed a strong sterilizing effect, and no alteration of the content of paeonol, color and morphology. Alcohol gas treatment resulted in similar effects as those in gamma irradiation. Collectively, these results suggest that appropriate sterilizing methods to guarantee the microbial quality of this herbal medicine may be those using gamma irradiation or alcohol gas.

Selection of Proper Medium and Amount of Applied Fertilizer for Exportable Cymbidium Young Plants Grown in Korea (수출용 심비디움 묘 생산에 적합한 배지 선발 및 시비량 구명)

  • Shim, Myung-Syun;Kim, Mi-Seon
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.217-222
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    • 2010
  • Bark is a general medium for potted Cymbidium in Korea, but it is difficult to shake off or eliminate the medium from the roots before exporting the potted plants. This working process can injure the roots and then deteriorate plant marketability. This study was carried out to select the optimum medium easier to eliminate from the roots instead of bark and the optimum amount of applied fertilizer to improve the plant growth. Cymbidium young plants 'Honey Hot' and 'Desert Look' were planted in pots with bark, cocochip, and peatmoss. The plants were treated with 2, 4, and 6 g of slow release fertilizer. The plant growth characteristics were investigated in the first and second years during production period of three years. The medium characteristics and mineral nutrient content of the leaves were also examined in the second year. In the first year, the plant growth of 'Desert Look' was improved in all peatmoss treatments more than bark. 'Honey Hot' showed the highest plant growth values in the bark treatment. In the second year, the plant growth of the two cultivars was improved in peatmoss. Cocochip treatments showed the lower plant growth values than bark and peatmoss in the first and second year. There was no significant difference among fertilizer amounts in all the media. The higher CEC values of peatmoss medium resulted to higher capacity to hold more nutrients than bark, and the nutrient retention of the peatmoss improved the plant growth. The higher K and Ca contents in the leaves would contribute to improve the plant growth. Consequently, it would be possible to use peatmoss instead of bark for Cymbidium young plants, but there must be always attention to appropriately water the medium and manage the moisture.

Investigation of Radial Distributions of Tangential Strains and of Moisture Contents within a Log Cross Section by Circumferential Slices

  • Choi, Jun-Ho;Lee, Nam-Ho
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.2
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    • pp.20-28
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    • 2008
  • This study was carried out to provide the so-called circumferential slicing method for investigating radial distributions of the tangential strains and of moisture contents within the log cross section (LC) of Kalopanax pictus during indoor drying it. While the heartwood showed an almost uniform moisture content distribution in the range of about 50~55% in case of the green wood, it has gradually decreased toward the outer side, showing about 19% of moisture content difference from the innermost slice. Although the moisture gradient along the radial direction has gradually become gentle as drying progresses, the sapwood of the outer side represented the moisture contents below the fiber saturation point after 24 hours of drying while the heartwood in the inner part showed the moisture contents higher than the fiber saturation point. The pith side was laid under the tensile stress after 24 hours of drying, and then gradually decreasing toward the bark side, and showed the distribution being switched again to the tensile stress on the bark side. As the drying has progressed, this trend got more intensified, and finally showed the U-shaped distribution model after 48 hours of drying. The circumferential slice test is considered to be suitable in quantitatively determining the tangential strains and moisture content within a LC.

Effects of CEPA on the Secretion of Lacquer and Bark Physiology of Lactree(Rhus verniciflua Stokes) (CEPA 처리(處理)가 옻나무의 칠액분비(漆液分泌) 및 수피생리(樹皮生理)에 미치는 영향(影響))

  • Choi, Tae Bong;Kim, Mahn Jo;Hyun, Jung Oh
    • Journal of Korean Society of Forest Science
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    • v.89 no.1
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    • pp.116-125
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    • 2000
  • This study was conducted to investigate the effects of CEPA(2-chloroethyl phosphonic acid) on bark physiology and anatomy of lactree(Rhus verniciflua Stokes). Sample trees of similar size and growth rate were selected from 7-year-old lactree plantation located in Hyengsung-kun, Kangwon-do. Lanolin pastes containing 0.1, 1, or 10% CEPA were put into the bark-removed hole made by corer(${\phi}1cm$) on the main stem at 1.2m above the ground on June 16, 1995. Five weeks after application of CEPA, bark thickness was markedly increased as a result of the increase in the amount of phloem and intercellular spaces, and correlated with the increased production of urushiol. By the application of 10% CEPA, bark thickness was increased approximately 2.5 times, and the urushiol content within bark was increased 2.8 times compared to that of untreated trees because CEPA stimulated the accumulation of urushiol within bark. Treatment of 10% CEPA also increased the size and the total number of secretory canals, and induced an increase in ray width. The phloem parenchyma cells of CEPA-treated trees were well-developed and closely packed with little intercellular space.

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Physicochemical Changes of Woody Charcoals Prepared by Different Carbonizing Temperature (탄화온도가 목탄의 물리·화학적 특성에 미치는 영향)

  • Jo, Tae-Su;Choi, Joon-Weon;Lee, Oh-Kyu
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.3
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    • pp.53-60
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    • 2007
  • Carbon content, properties of micro-pore, and chemical properties of the charcoal prepared from wood powder, wood fiber, and bark of Abies sibirica Ledeb at different temperatures were investigated. The yield of charcoal decreased with increasing the carbonization temperature. The yield of bark charcoal was higher than those of wood and wood fiber charcoal. The content ratio of carbon atom in the charcoal increased with increasing the carbonization temperature, whereas those of hydrogen and oxygen atom were decreased. Ash content of bark charcoal was also higher than those of wood and wood fiber charcoal. The specific surface area of wood and wood fiber charcoal was greater than that of bark charcoal. In all charcoal, the specific surface area and the volume of micro-pore were highest when the carbonization temperature was $600^{\circ}C$, however they tended to decrease when the temperature was reached to $800^{\circ}C$. For the functionality test of chemical groups on the charcoal surface, adsorption test have performed against acidic (HCl) and basic chemicals (NaOH, $Na_2CO_3$, and $NaHCO_3$). As carbonization temperature increased, adsorption amount of HCl increased, while adsorption amounts of NaOH, $Na_2CO_3$, and $NaHCO_3$ were decreased. The charcoal prepared at higher temperature showed basic properties, while the charcoals manufactured at lower temperature presented acidic properties. Therefore, it was considered that the carbonization temperature affected the pH of charcoal.

Carbon Storage in Aboveground, Root, and Soil of Pinus densiflora Stand in Six Different Sites, Korea

  • Park, Gwan-Soo;Choi, Jaeyong;Lee, Kyung-Hak;Son, Young-Mo;Kim, Rae-Hyun;Lee, Hang-Goo;Lee, Sang-Jin
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.12 no.2
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    • pp.1-9
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    • 2009
  • Due to the increase of carbon dioxide in the atmosphere and global warming, the importance of forest ecosystems, as a place of carbon accumulation and emission, has received a great amount of recognition lately. This study was performed to help understand and provide the current status of carbon cycle in the pinus densiflora stand, Korea. The samples were collected from average 35-years-old Pinus densifiora rands in Gongju, Youngdong, Chungsan, Muju, Mupung, and Jangsu regions. Total thirty aboveground sample trees were cut, and ten roots were sampled, and soil samples were collected. Average carbon concentrations in foliage, branch, stem bark, stem wood, and root were 55.7%, 56.0%, 56.0%, 57.3%, and 56.5%, respectively. Carbon content was estimated by the model $Wt=aD^b$ where Wt is oven-dry weight in kg and D is DBH in cm. Total carbon content (aboveground and root) was 42.39tonC/ha in the Pinus densiflora stand. The proportion of each tree component to total carbon content was high in order of stemwood, root, branch, stem bark, and foliage. Total net primary production (aboveground and root) was estimated at 6.51tonC/ha/yr in Pinus densiflora stand. The proportion of each tree component to total net primary carbon content was high in order of sternwood, root, branch, foliage and stembark. Soil carbon contents in the study sites was 43.51tonC/ha at 0-50cm soil depth.

Quantitative Determination on the Constituents of the Stem Bark and the Leaf Shoot of Kalopanax pictus by HPLC Analysis (HPLC 분석에 의한 해동피와 개두릅의 성분함량 비교)

  • Kim, Min-Young;Yoo, Yeong-Min;Nam, Jung-Hwan;Choi, Jong-Won;Park, Hee-Juhn
    • Korean Journal of Pharmacognosy
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    • v.38 no.3 s.150
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    • pp.270-276
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    • 2007
  • To evaluate the leaf shoots and stem barks of Kalopanax pictus (Araliaceae) as an edible vegetable and a traditional medicinal drug, respectively, the compounds of syringin, liriodendrin, astragalin, quercetin, and kalopanaxsaponins were quantitatively measured by HPLC analysis. The leaf shoot exhibited low contents of syringin, liriodendrin and kalopanaxsaponins but a high chlorogenic acid content, whereas the grown leaves contained very high amounts of kalopananxsaponins. In contrast, the stem bark had very high amounts of syringin and liriodendrin and relatively low kalopanaxsaponins. In particular, the kalopanaxsaponin contents were rapidly increased with monthly variation until October but decreased from September. It was also observed that the leaf shoot contained chlorogenic acid by 30.73 mg/g and the barks showed the concentration of liriodendrin by 20.75 mg/g. These results indicate that high contents of syringin and liriodendrin in the stem bark and high contents of chlorogenic acid in the leaf shoot support scientific bases on the traditional uses of K. pictus as a medicinal drug and a functional food, respectively.

Utilization of Pine Bark(III) - Characterization of Spent Liquor Obtained from Bisulfite Cooking of Pine Bark - (소나무 수피(樹皮)의 총합적(總合적) 이용(利用)(III) - 수피(樹皮) 중아황산염(重亞黃酸鹽) 증해폐액(蒸解廢液)의 특성(特性) -)

  • Mun, Sung-Phil
    • Journal of the Korean Wood Science and Technology
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    • v.22 no.2
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    • pp.54-60
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    • 1994
  • The pine bark from mechanical pulp industry was cooked with bisulfite liquor, and the chemical and physical properties of the spent liquor after cooking for 90 minutes, which allowed partial defibration were determined. Lignosulfonate or lignin-related materials in the spent liquor have a molecular weight of approximately 3,000, which is lower than that of commercial lignosulfonate (C-LS) prepared from wood. The sulfonic equivalent weight calculated by colloid titration was 307 for C-LS, 238~239 for spent liquor. The contents of lignosulfonic acid and phenolic hydroxyl groups on $C_9$ unit of lignin in the spent liquor were 0.98~0.99, and 1.51~1.66, respectively. These values were remarkably higher than those of C-LS. Especially the content of phenolic hydroxyl group was almost twice that of C-LS. The viscosity of spent liquor after freeze drying was very low compared to C-LS. The dispersing ability of spent liquor was almost equivalent to or more than that of C-LS.

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