• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.103-103
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• 2003

The purpose of this study is to evaluate an efficacy of in vitro differentiated human embryonic stem (hES, MB03) cells expressing Nurr1 in relief of symptomatic motor behavior of Parkinson's disease (PD) animal models MB03 was genetically modified to express Nurr1 protein and was induced to differentiate according to 2-/4+ protocol using retinoic acid and ascorbic acid. The differentiation-induced cells were selected for 10 to 20 days thereafter in N2 medium. Upon selection, cells expressing GFAP, TH, or NF200 were 38.8%, 11%, and 20.5%, respectively. in order to examine therapeutic effects of the differentiated cells in PD animal model, rats were unilaterally lesioned by administration of 6-kydroxydopamine HCI (6-OHDA) into medial forebrain region (MFB, AP -4.4 mm, ML 1.2 mm, DV 78 mm with incision bar set at -2.4 mm), as a reference to bregma and the surface of the skull. Confirmation of successful lesion by apomorphine-induced rotational behavior, differentiated cells were transplanted into the striatum (AP 1.0, ML 3.5, DV -5.0; AP 0.6, ML 2.5, DV -4.5). Improvements of asymmetric motor behavior by the transplantation were examined every two weeks after the surgery. In two weeks, numbers of rotation by the experimental rats were $-14.8 \pm 33.9%$ (P<0.05) of the number before transplantation, however, the ratio increased slightly to $13.6 \pm 56.3%$ in six weeks. In contrast, the ratio of sham-grafted animals ranged from 112.3+8.5% to 139.2+28.9% during the examination. Immunohistochemical studies further confirmed the presence, survival, migration, and expression of TH of the transplanted human cells.

• 한국의사학회지
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• 제32권1호
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• pp.63-76
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• 2019

The $S{\bar{u}}tra$ of Great Dhāraṇī of the Uṣṇīṣa-cittā is one of the most widely published scriptures of Esoteric Buddhism during the Goryeo and Joseon dynasty. The sutra is often referred to as a Buddhist medical scripture whose recitation is believed to allay diseases such as difficult birth, chest pain, and infectious and incurable diseases. It utilizes two major methods for healing: reciting and copying the sutra or copying the dharani and talisman which appear at the end of the text with fine cinnabar and ingesting them with aromatic water. In order to understand the medical and historical value of this sutra, this study examines the historical background of its establishment in early China and its migration to Korea in the Goryeo period. This study also investigates the identity and value of the scripture in Buddhist medical history. The oldest known printed texts of the sutra in Korea are dated to the late 11th century. Because of the material benefits of the text, the tradition of printing and distributing the sutra was very popular between the 13th and 19th centuries. Therefore, examples of the sutra having been used for medicinal purposes during rites are found in historical records. Finally, the study tries to identify the relationship between the treatment methods of the Sutra and those of Korean traditional medicine focusing on several Korean traditional medical texts such as the Hyangyak-Jibseongbang (Compilation of Native Korean Prescriptions), the Donguibogam, and the Euibang-Hapbu.

• 한국습지학회지
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• 제15권2호
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• pp.251-264
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• 2013

도요 물떼새들은 만조시에 해안 내륙 휴식지에서 휴식을 취하는데, 휴식지를 선택할 때 여러 가지 요소들을 고려한다. 우리는 도요 물떼새 8종(알락꼬리마도요 Numenius madagascariensis, 청다리도요 Tringa nebularia, 큰뒷부리도요 Limosa lapponica, 개꿩 Pluvialis squatarola, 민물도요 Calidris alpine, 중부리도요 Numenius phaeopus, 마도요 Numenius arquata, 뒷부리도요 Xenus cinereus)을 대상으로 휴식지의 시공간적 이용과 그 이용에 영향을 미치는 요인들을 분석하였다. 휴식지 내 고인 물의 면적과 길이, 기온, 풍속, 종별로 이동시기, 방해정도를 변수로 고려하였다. 시공간적으로, 이용하는 종이 달랐으며 개체수 또한 변동을 거듭하였다. 그리고 도요 물떼새의 휴식지 이용에 영향을 미치는 요인은 종-특이적이었다. 연구지역의 휴식지는 폐장한 새우양식장이었으나 조사 기간 중 염생습지에서 자라는 함초 Salicornia herbacea 농장으로 전환하였다. 휴식지가 필요한 상황에서, 도요 물떼새들의 생태를 기초로 해서 기존 휴식지를 관리할 것을 권한다. 더불어, 여름철 가뭄에는 양식장의 수문을 열어주어 도요 물떼새들이 물을 이용해 체온관리를 할 수 있게 해주고, 만조시 도요 물떼새들이 양식장 안으로 날아 들어올 수 있도록 새들이 휴식지를 찾기 시작하는 만조 전네 시간부터는 농장 작업을 안 할 것을 권한다.