• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.103-103
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• 2003

The purpose of this study is to evaluate an efficacy of in vitro differentiated human embryonic stem (hES, MB03) cells expressing Nurr1 in relief of symptomatic motor behavior of Parkinson's disease (PD) animal models MB03 was genetically modified to express Nurr1 protein and was induced to differentiate according to 2-/4+ protocol using retinoic acid and ascorbic acid. The differentiation-induced cells were selected for 10 to 20 days thereafter in N2 medium. Upon selection, cells expressing GFAP, TH, or NF200 were 38.8%, 11%, and 20.5%, respectively. in order to examine therapeutic effects of the differentiated cells in PD animal model, rats were unilaterally lesioned by administration of 6-kydroxydopamine HCI (6-OHDA) into medial forebrain region (MFB, AP -4.4 mm, ML 1.2 mm, DV 78 mm with incision bar set at -2.4 mm), as a reference to bregma and the surface of the skull. Confirmation of successful lesion by apomorphine-induced rotational behavior, differentiated cells were transplanted into the striatum (AP 1.0, ML 3.5, DV -5.0; AP 0.6, ML 2.5, DV -4.5). Improvements of asymmetric motor behavior by the transplantation were examined every two weeks after the surgery. In two weeks, numbers of rotation by the experimental rats were $-14.8 \pm 33.9%$ (P<0.05) of the number before transplantation, however, the ratio increased slightly to $13.6 \pm 56.3%$ in six weeks. In contrast, the ratio of sham-grafted animals ranged from 112.3+8.5% to 139.2+28.9% during the examination. Immunohistochemical studies further confirmed the presence, survival, migration, and expression of TH of the transplanted human cells.

• The Journal of Korean Medical History
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• v.32 no.1
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• pp.63-76
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• 2019

The $S{\bar{u}}tra$ of Great Dhāraṇī of the Uṣṇīṣa-cittā is one of the most widely published scriptures of Esoteric Buddhism during the Goryeo and Joseon dynasty. The sutra is often referred to as a Buddhist medical scripture whose recitation is believed to allay diseases such as difficult birth, chest pain, and infectious and incurable diseases. It utilizes two major methods for healing: reciting and copying the sutra or copying the dharani and talisman which appear at the end of the text with fine cinnabar and ingesting them with aromatic water. In order to understand the medical and historical value of this sutra, this study examines the historical background of its establishment in early China and its migration to Korea in the Goryeo period. This study also investigates the identity and value of the scripture in Buddhist medical history. The oldest known printed texts of the sutra in Korea are dated to the late 11th century. Because of the material benefits of the text, the tradition of printing and distributing the sutra was very popular between the 13th and 19th centuries. Therefore, examples of the sutra having been used for medicinal purposes during rites are found in historical records. Finally, the study tries to identify the relationship between the treatment methods of the Sutra and those of Korean traditional medicine focusing on several Korean traditional medical texts such as the Hyangyak-Jibseongbang (Compilation of Native Korean Prescriptions), the Donguibogam, and the Euibang-Hapbu.

• Journal of Wetlands Research
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• v.15 no.2
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• pp.251-264
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• 2013

There are some factors affecting the shorebirds in selecting a coastal inland roost site where they have to stay during the high tide when the entire intertidal zone is covered with water. We investigated eight species (Eastern Curlews Numenius madagascariensis, Green Shanks Tringa nebularia, Bar-tailed Godwits Limosa lapponica, Grey Plovers Pluvialis squatarola, Dunlins Calidris alpine, Whimbrels Numenius phaeopus, Eurasian Curlews Numenius arquata and Terek Sandpipers Xenus cinereus) to identify the spatial and temporal variability in coastal inland roost use in the area and the factors influencing the use. We considered the area and length of the standing water in the roost site, temperature, wind speed, the time of migration and the intensity of disturbance. As a result, number of individuals and type of species using the roost site varied across spatial and temporal scales. And the factors affecting the roost use was species-specific. The roost site of the study area was a closed shrimp farm however, it has been converted to a Salicornia herbacea, a salt marsh plant, farm recently. In a situation where coastal inland roost site is needed, we hereby describe a resolution for the juxtaposition of shorebirds and farming.