• Title/Summary/Keyword: bank filtration

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Purification and Characterization of Methyl Mercaptan Oxidase from Thiobacillus thioparus for Mercaptan Detection

  • Lee, Hyun-Ho;Kim, Sang-Joon;Shin, Hyun-Jae;Park, Ji-Yeon;Yang, Ji-Won
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.6
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    • pp.375-379
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    • 2002
  • Methyl mercaptan oxidase was successfully induced in Thiobacillus thioparus TK-m using methyl mercaptan gas, and was purified for the detection of mercaptans. The purification procedure Involved a DEAE (diethylaminoethyl) -Sephacel, or Superose 12, column chromatography with recovery yields of 47.5 and 48.5%, and specific activities of 374 and 1240.8 units/mg-protein, respectively, The molecular weight of the purified methyl mercaptan oxidase was 66.1kDa, as determined by SDS-PAGE. The extract, from gel filtration chromatography oxidizes methyl mercaptan, producing formaldehyde, which can be easily detected by the purpald-coloring method. The optimized temperature for activity was found to be at 55$\^{C}$. This enzyme was inhibited by both NH$_4$Cl and (NH$_4$)$_2$SO$_4$, but was unaffected by either KCl or NaCl at less than 200 mM. With K$_2$SO$_4$, the activity decreased at 20 mM, but recovered at 150 mM. In the presence of methanol, full activity was maintained, but decreased in the presence of glycerin, ethanol and acetone 43, 78 and 75%, respectively.

Draw-down Characteristics of Bank Filtration by Using the Horizontal Collector Well (방사형집수정에 의한 강변여과수 수위하강 특성)

  • Chung Ji Hoon;Park Jae Hyun;Park Chang Kun;Yang Jung Suk
    • Proceedings of the Korea Water Resources Association Conference
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    • 2005.05b
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    • pp.240-244
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    • 2005
  • 현재 강변여과수 개발은 수직정 위주로 개발되어졌으나, 수직정을 이용한 취수 방식은 산출량에 대한 한계를 가지고 있어 대용량 취수를 위해서 많은 취수정 착정이 불가피하다. 취수정 증가와 더불어 유지관리상의 문제점을 가지고 있으며 실제 운영에서 문제점이 나타나고 있는 실정이다. 이러한 수직정 취수정의 문제점을 해결하기 위해 방사형 집수정을 이용한 강변여과수 개발이 시도 되고 있다. 현재 창원시 대산면 일대에서 국내최초로 방사형집수정을 이용한 강변여과수 개발이 진행되고 있으며, 현재 방사형 취수정 1기가 시공 완료되어 시험 가동 중에 있다. 방사형 집수정에 의한 강변여과수 개발은 대용량 취수를 목적으로 설치되기 때문에 산출량뿐만 아니라 집수정 인근의 수위하강에 특성이 중요한 요소 중에 하나이다. 본 연구에서는 창원시 대산면 일대에서 진행되고 있는 방사형 집수정 설치에 따른 강변여과수 산출시 집수정 인근 수위하강 특성 파악을 위한 모니터링을 목적으로 실시하였다. 수위하강 특성 관측을 위해 양수실험을 위해 대용량 양수펌프 설치, 유량검증을 위한 대형 웨어 설치, 수위변화 측정을 위해 집수정 30m 반경에 8개의 관측정을 착정하여. 디지털 수위측정기를 설치하여 수위하강 특성을 모니터링 하였다. 수위 모니터링 결과 취수정의 수위하강 특성과 유사하게 수평정의 수위하강 경향 나타났으며, 강변에 가까운 수평정의 수위하강은 약 $30\% 감소하였으며, 집수정 양수량에 변화에 따라 민감하게 반응하지 않는 특성을 나타냈다.

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Calculation of the Yeild of Bank Filtration by Using the Horizontal Collector Well (방사형집수정에 의한 강변여과수 산출량 산정)

  • Chung Ji Hoon;Park Jae Hyun;Park Chang Kun;Yang Jung Suk
    • Proceedings of the Korea Water Resources Association Conference
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    • 2005.05b
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    • pp.250-254
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    • 2005
  • 국내의 상수원수 확보는 대부분을 표류수에 의존하고 있으나, 표류수의 경우 수질오염의 증가와 돌발 수질 오염 사고에 대한 대응 등의 취약점을 가지고 있다. 이러한 문제점을 해결하기 위해 근본적으로 깨끗한 상수원수를 확보하기 다양한 방법이 모색되어지고 있다. 수량적으로 안정적이고, 수질적으로 안전한 상수원수 취득 방법으로 강변여과수, 지하댐, 인공함양기법 등이 실질적으로 계획되어 시행되어지고 있다. 특히 강변 충적층의 자연정화 기능을 이용한 강변여과수 개발은 지하수 개발과 달리 수량적으로 안정적이고, 수질적으로 안전한 강변여과수 개발 방안이 적극적으로 진행되고 있다. 낙동강 하류 지역인 함안군, 창원시, 김해시 등은 대부분 하천 표류수에 의존하였으나, 유역내 충적층 발달 지역이 분포해 있고, 하상경사, 하상계수 등의 하천공학적 측면에서 강변여과수 개발에 유리한 입지조건을 가지고 있어 강변여과수 개발이 진행되고 있다. 현재 창원시 대산면 일대에서 국내최초로 방사형집수정을 이용한 강변여과수 개발이 진행되고 있으며, 현재 방사형 취수정 1기가 시공 완료되어 시험 가동 중에 있다. 본 연구에서는 창원시 대산면 일대에서 진행되고 있는 방사형 집수정 설치에 따른 양수시험을 실시하여 양수량에 따른 수위하강 겋항 등을 분석하여 적정 산출량을 결정하였고, 계획단계에서 적정 산출량 예상시 사용한 경험식(Petrovic, Milojevic 경험식)의 적합성 여부에 중점을 두어 진행 되었다. 연구대상 지역인 창원시 대산면 일대는 지하수위가 하천수위보다 높은 이득하천의 형태로 건기시 하천특성을 뚜렷하며, 양수실험을 위한 대용량 양수펌프 설치, 유량검증을 위한 대형 웨어 설치, 수위변화 측정을 위한 디지털 수위계 등을 설치하여 실험을 수행하였다. 단계/장기양수시험을 통하여 양수량-수위하강 경향을 분석하여 방사형 집수정 1기의 적정 양수량은 약 $16,000m^3/day$로 예상하였으며, 계획 시 사용한 경험식(Milojevic)으로 산정된 산출예상량과 유사한 특성을 나타내었다.

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Trend and Barrier in the Patents of Artificial Recharge for Securing Goundwater (지하수자원 확보를 위한 인공함양 기술 특허동향 및 장벽 분석)

  • Kim, Yong-Cheol;Seo, Jeong-A;Ko, Kyung-Seok
    • Journal of Soil and Groundwater Environment
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    • v.17 no.3
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    • pp.59-75
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    • 2012
  • It is getting difficult to manage water resources in South Korea because more than half of annual precipitation is concentrated in the summer season and its intensity is getting severe due to global warming and climate change. Artificial recharge schemes can be a useful method to manage water resources in Korea adapting to climate change. Patent analysis enables us to prevent overlapping investment and to find out unoccupied technology. In this study, international patent trends and barriers of artificial recharge technology are analysed for patents of Korea, Japan, the United States and Europe. The four artificial recharge methods such as well recharge, surface infiltration, bank filtration and underground structures are classified as main class and the nine sub-technologies such as water intake, water treatment, injection wells, monitoring of groundwater flow, groundwater pumping, surface infiltration/soil aquifer treatment, radial collection well, iron/manganese treatment, and underground subsurface dam are classified as intermediate class. Water intake techniques are subdivided into five classifications. Total 1,281 of patents, searched by WIPS DB tool and selected after removing noisy patents, are analyzed quantitatively to evaluate application trends by year, applicant, country for each classified technologies and analyzed qualitatively to find out occupied and unoccupied technologies. It is expected that upcoming research and development project could be performed efficiently in that an avoidance plan for the similar patents and differentiation plan for the advancing patents are set up based on the quantitative and qualitative analysis results from this research.

Subsurface Water Storage Using Coastal Aquifers Filled With Saline Water (염수로 포화된 해안지역 대수층을 활용한 수자원확보 방안)

  • Jung, Eun Tae;Park, Namsik;Kim, In Chul;Lee, Seoung Hwi
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.27 no.5
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    • pp.353-357
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    • 2015
  • A new method is proposed for water resources using aquifers in coastal areas. These aquifers are generally filled with saline water due to seawater intrusion and consequently being left unutilized. Surface water can be injected into these aquifers and recovered for water quality enhancement and stored water. Injection and pumping wells are used. For this technique to be successful protection of pumping well from seawater intrusion is an essential issue. Salt water pumping can be used to prevent saline water upconing. Numerical analysis demonstrated that a properly designed and executed salt water pumping well can protect a freshwater pumping well from salt water intrusion.

In situ iron/manganese removal and permeability improvement at the river bank filtration site (강변여과수내 철망간 저감 및 투수성 개선)

  • Kim, Byung-Woo;Kim, Byung-Goon;Hur, Young-Teck;Kim, Dong-Sup;Kim, Hong-Suck
    • Proceedings of the Korea Water Resources Association Conference
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    • 2018.05a
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    • pp.228-228
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    • 2018
  • 강변여과는 지하수 인공함양 방식 중 유도함양(induced recharge) 또는 간접함양 방식에 속한다. 이는 하천 및 강변 부근에 집수시설을 설치한 후, 미고결층 대수층(unconsolidated aquifer)의 자연 오염 저감능을 이용하여 지표수를 간접 취수하는 방식으로 수질이 불량한 지표수가 대수층을 관류하면서 희석, 화학적 이온 교환 및 반응, 흡착, 생물막(biofilm; 미생물에 의한 자연저감), 여과 등을 통하여 수질이 개선된다. 강변여과수내의 용존 농도가 높은 철과 망간은 수처리 비용증가, 용수관정 및 시설물의 수명단축을 초래한다. 따라서 강변여과 지역의 미고결 대수층에서 효과적인 철과 망간 동시 제거(vyredox)를 위해 에어서징(air surging)과 블록 서징(block surging)을 실시하기 위해서 실내 물탱크 모델(water tank model)에서 에어서징에 따른 공기 순환 우물시스템을 관찰하였으며, 이를 바탕으로 현장시험(Test bed)에 적용하였다. 미고결 대층수층에서의 철 망간은 음용수 기준치(각각 0.3 mg/L)를 초과하고 있으며, 강변여과 취수 개발 및 이용을 제한하는 요인이 되고 있다. 본 연구에서 사용된 에어서징과 블록서지 기술은 자갈층 및 미고결 대수층에 충진된 슬라임 및 폐색(clogging)을 제거함과 동시에 관정 주변의 대수층의 투수성 개선과 산화환경으로 치환되며, 대수층에 잔존하는 철/망간의 산화물들을 관정내로 빼낼 수 있는 방법이다. 따라서 서징에 따른 폐색 제거효율을 검토한 결과에서 철 망간 이온농도 저감효과와 관정 주변의 수리전도도(hydraulic conductivity) 및 저류계수(coefficient of storage)가 증가한 것으로 나타났다. 이와 같이 강변여과에 의한 폐색은 미고결층내 공기주입 및 블록서지를 통하여 철/망간 이온농도 저감 및 수리특성 개선 효과에 유용한 것으로 평가된다.

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Diversity of Fungi in Brackish Water in Korea (국내 기수역 환경의 균류 다양성)

  • Jeon, Yu Jeong;Goh, Jaeduk;Mun, Hye Yeon
    • The Korean Journal of Mycology
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    • v.48 no.4
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    • pp.457-473
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    • 2020
  • We investigated the distribution and diversity of fungi in brackish water and soil from the Eulsukdo Island, Geumgang Estuary Bank, Suncheon Bay, Dae-ho Tide Embankment and coastal sand dune in Sinduri and Bu-nam Tide Embankment, Korea. Fungi were isolated from water samples by hand-pumped filtration, and soil samples were collected and diluted. The isolated fungi were incubated in potato dextrose agar at 25℃. A total of 173 fungal strains were isolated from brackish water and identified according to their respective internal transcribed spacer via phylogenetic analysis. The diversity of all fungal strains was analyzed according to diversity indices. The fungal strains belonged to any of 18 taxonomic orders: Pleosporales, Eurotiales, Capnodiales, Hypocreales, Polyporales, Saccharomycetales, Agaricales, Glomerellales, Mucorales, Dothideales, Russulales, Xylariales, Sordariales, Myrmecridiales, Tubeufiales, Onygenales, Cantharellales, and Amphisphaeriales. Cladosporium spp. (20%), Penicillium spp. (19%), and Fusarium sp. (5%) comprised majority of the identified strains. Two species from the fungal isolates were newly identified in Korea: Sarocladium kiliense NNIBRFG3280 and Fusicolla merismoides NNIBRFG23708.

Current States of the Global Water Market and Considerations for the Groundwater Industry in South Korea (물 시장의 현주소와 지하수 산업에 대한 고찰)

  • Kim, Byung-Woo;Koh, Yong-Kwon;Choi, Doo-Houng;Kim, Deog-Geun;Kim, Gyoo-Bum
    • The Journal of Engineering Geology
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    • v.24 no.3
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    • pp.431-440
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    • 2014
  • Since the establishment of the Groundwater Act in Korea in 1993, the national policy on groundwater has focused on the preservation and management of groundwater, which should be used only as a subsidiary water resource. However, population growth, increased water demand, climate change, and the need for uniform water distribution have brought changes to groundwater policy, and have led to the prioritization of development projects such as groundwater dams and river bank filtration. Population growth, changes to the water environment, and increased water risks have all played a role in triggering rapid growth within the water industry; the size of the investment in water resources will also continue to increase worldwide. Until now, private wells and bottled mineral water have led the groundwater industry in South Korea. However, a new area of the groundwater industry, which includes the health and medical sciences, employs groundwater properties derived from regional geology, and is growing. This requires the advancement of groundwater research and technical development connected with ICT (Information and Communication Technology) and medical science, and that the public development of groundwater and its various applications is expanded through locating groundwater in the core of the water industry cluster.

Study on Permeability, Optimum Yield and Long-term Stability in Alluvial Well with Filter Layer Change (충적우물에서 필터층 변화에 따른 투수특성, 적정양수량 및 장기적 안정성에 대한 연구)

  • Song, Jae-Yong;Lee, Sang-Moo;Choi, Yong-Soo;Kim, Ki-Joon;Jeong, Gyo-Cheol
    • The Journal of Engineering Geology
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    • v.28 no.1
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    • pp.101-115
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    • 2018
  • This study was carried out to evaluate the effects of various filter conditions on unconfined aquifer (alluvial aquifer). We made model test device which has filter layer, pumping well and observation well which consist of sand layer and gravel layer to test. Step drawdown test and long term pumping tests were carried out using the device. The permeability characteristics of each test group were confirmed and the optimal yield was calculated. As a result of comparing the optimal yield of double filter and single filter in sand, dual-filter SD-300 was valued at 216.8 % higher final optimal yield than single-filter SS-300. Comparing the dual filter SD-300 and the single filter SS-100 with a thin filter layer, dual-filter SD-300 was valued at 709.2% higher final optimal yield than single-filter SS-300. As a result of analysis of optimal yield change over time, It was confirmed that the ratio of optimal yield of single filter and dual filter increase over time. In order to evaluate the long-term change in water intake efficiency, we considered the point at which the initial optimal yield was reduced by 50%. The dual filter SD-300 is about 351.1% higher than SS-300, which is the same thickness filter, and about 579.0% higher than SS-100. From these results, Assuming that the point at which the initial quantity of water intake is reduced to 50% is the well life, double filters are expected to increase their lifespan by about 3.5 times over single filters of the same thickness and by about 5.8 times over typical single filter. These results can be used to design wells to river bank filtration or filtered seawater. In addition, it is possible to clarify the effect of the double filter through the comparison with the future field test results.