• Title/Summary/Keyword: bacteriological

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Bacteriological Studies on Liver Abscess of Cattle by the Gas Jet Anaerobic Culture Method (Gas 분사장치(噴射裝置)에 의(依)한 혐기성배양법(嫌氣性培養法)을 이용(利用)한 소 간농양(肝膿瘍)의 세균학적(細菌學的) 연구(硏究))

  • Mah, Jum-Sool
    • The Journal of the Korean Society for Microbiology
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    • v.11 no.1
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    • pp.79-85
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    • 1976
  • There are many of anaerobic culture methods and equipments for isolation and cultivation of anaerabic bacteria, but most of these methods are used without pre-reduced media. Gas-jet method is a recommend. able method for the culture of anaerobes, resently developed. Bacteriological studies were experimented of liver abscess of cattle by the use of gas. jet method. The results were summarised as follows; 1. Gas-jet method for anaerobic culture are expedient for the making of pre-reduced media, maintaining of oxygen free condition in the culture tube, picking of bacteria from colony and colony counting etc. 2. A 121 strains of facultative anaerobic and anaerobic bacteria were isolated from liver abscess of 27 head of cattle, and the isolated anaerobic bacteria were as follows. Peptostreptococcus spp. 7 strains Acid aminococcus fermentans 1 Veillonella spp. 1 Bacterioides spp. 6 Bifidobacterium spp. 4 Arachinia propionica 2 Lactobacillus spp. 4 Propionibacterium acnes 1 3. Liver abscess were infected with many of bacteria, about $10^3-10^9$ numbers per gram of abcessed tissue. Almost of abscess were mixed infection of various bacterial species rather than simple species.

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Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction

  • Ahn, Jong Gyun;Choi, Seong Yeol;Kim, Dong Soo;Kim, Ki Hwan
    • Clinical and Experimental Pediatrics
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    • v.55 no.11
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    • pp.424-429
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    • 2012
  • Purpose: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae -positive results. Methods: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. Results: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. Conclusion: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes.

A Pathological and Bacteriological Study on the Mammary Glands of Korean Native Cows Slaughtered in Gwangju Area (광주지역에서 도태되는 한우 유방의 병리학적 및 세균학적 연구)

  • 이정치;김혜라;이채용;이정길
    • Journal of Veterinary Clinics
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    • v.21 no.3
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    • pp.280-285
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    • 2004
  • Pathological and bacteriological studies were made of a total of 111 udders from slaughtered Korean native cows originating from various locations in Chonnam province. Incidence of supernumerary teats was 8.1%. Gross lesions were found in 21.4% of mucous membranes of total teat cisterns; these lesions comprised epithelial hyperplasia (12.6%), congestion (4.1%), slight fibrosis (3.8%) and severe fibrosis with congestion (0.9%). Gross lesions in the glandular tissues were found in 35.1% of cows; these lesions comprised slight fibrosis (27.9%), moderate fibrosis (6.3%) and severe fibrosis with pus (0.9%). Mastitis was found only in one cow. One hundred and twenty-five strains of microorganisms were isolated from mammary gland fragments of the cows. Pathogens most commonly isolated were Staphylococcus spp.; most of them were minor mammary pathogens. Major mammary pathogens were Streptococcus spp. (9.6%) and E. coli (2.4%). Most of the isolates were susceptible to many of 13 antimicrobial agents used, but Streptococcus spp. and Gram-negative rods showed resistance to 7 and 8 agents, respectively. 97.5% of the isolates showed susceptibility to amoxicillin, but only 55.8% to erythromycin.

Bacteriology and Antibiotic Sensitivity for Diabetic Foot Ulcer (당뇨족 궤양의 세균 역학과 항생제 감수성)

  • Choi, Sang-Rok;Lee, Chang-Kyu;Kim, Deok-Woo;Han, Seung-Kyu;Kim, Woo-Kyung
    • Archives of Plastic Surgery
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    • v.33 no.3
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    • pp.330-334
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    • 2006
  • Polymicrobial nature of diabetic foot infection has been well documented in the literature. Initial antibiotic therapy of diabetic foot infection is usually empiric until reliable culture data is shown. This study was carried out to determine the common bacteriological flora of diabetic foot infection and antimicrobial sensitivity pattern in order to enhance possible empiric treatment. The specimens were obtained from wounds of 207 cases of diabetic foot ulcer, and the bacteriological isolation, and antimicrobial susceptibility tests of the isolates were carried out by standard microbiological methods. Staphylococcus aureus was the most common isolate, with 46.2% of recover rate among total bacterial isolated cases. Among gram-negative organisms, Pseudomonas aeruginosa was most common. Gram-positive organisms showed significant susceptibility to clindamycin, trimethoprim/sulfamethoxazole, and levofloxacin, besides vancomycin. Cefoperazone, piperacillin/tazobactam, and amikacin in addition to imipenem were most effective agents compared to gram-negative organisms. Diabetic foot infection requires use of combined antimicrobial therapy for initial management. Our results indicate that the most effective antibiotic combination for diabetic foot infection of Korean patients is clindamycin plus cefoperazone.

Clinical and bacteriological studies on infectious atrophic rhinitis of swine (돼지 전염성 위축성 비염의 임상학적 및 세균학적 연구)

  • Kim, Bong-hwan;Tak, Ryun-bin;Cho, Gil-jae;Jang, Hee-kyung
    • Korean Journal of Veterinary Research
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    • v.31 no.4
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    • pp.457-469
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    • 1991
  • Clinical and bacteriological observations on infectious atrophic rhinitis of swine were conducted in order to obtain some basic information for the clinical and immnnological control of the disease prevailing in the republic. Samples were collected from nasal cavities of 135 4~12 week old pigs from 12 herds and from turbinates of 199 slaughtering pigs from 14 swine herds to investigate the prevalence of Bordetella bronchiseptica and/or Pasteurella multocida in the nasal cavities of the pigs. On the examination of nasal swabs by cultural techniques and of gross lesion of snouts of slaughtering pigs, all the swine herds investigated were found to be affected by atrophic rhinitis and a total of 84 B bronchiseptica and 139 P multocida cultures were isolated from the nasal cavities of the pigs. Of the 199 slaughtering pigs, some 48% revealed gross pathological lesion typical of atrophic rhinitis and the prevalence of B bronchiseptica and P multocida were 27.6% and 46.7%, respectively. Biochemical properties, antimicrobial susceptibilities, serological characteristics and toxigenicity of the isolates of B bronchiseptica and P multocida were investigated.

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Current Status of Bacterial Brown Stripe of Rice Caused by Acidovorax avenae subsp. avenae (Acidovorax avenae subsp. avenae에 의한 세균성줄무늬병의 연구동향)

  • 송완엽
    • Plant Disease and Agriculture
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    • v.5 no.2
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    • pp.69-76
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    • 1999
  • Acidovorax avenae subsp. avenae is the causal pathogen of several hosts including oats corn foxtail millet wheatgrass sugarcane and rice. The pathogen is a seedborne pathogen of rice and known to occur widely in rice growing countries. The pathogen cause inhibition of germination brown stripe on the leaf curling of the leaf sheath and abnormal elongation of the mesocotyl of irce. Bacterial colonies grow slowly and are convex circular and creamy with tan to brown center. The causal baterium is Gram-negative and rod shape with a single polar flagellum Nonfluorescence poly-$\beta$-hydroxybutyrate accumulation and precipitate formation around the colony on the medium are useful in the differentiation of this bacterium from other subspecies of A. avenae as well as nonfluorescent bacteria pathogenic to rice. This bacterium has belonged to the genus of Psdeudomonas but recently was transferred to the new genus Acidovorax on the basis of bacteriological and molecular biological data. However the difference of biochemical characteristics protein profile of the cell and host range among strains should be more clarified. To develop an effective control strategy for this disease understanding of detailed life cycle of the disease ritical environmental factors affecting disease development on each host and relationship to grain discoloration of rice are prerequisite. Although the affected area has been world-widely reported there is on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.

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Flavonoids as a Possible Preventive of Dental Plaque

  • Ammar-Nagwa;El, Diwany-Ahmed;Nagwa-Osman;Soheir-Gaafar;Nagwa-Amin
    • Archives of Pharmacal Research
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    • v.13 no.2
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    • pp.211-213
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    • 1990
  • To test flavonoids for antibacterial activity against oral micraorganisms, flavonoids, quercetrin and naringenin, were incorporated into two pharmaceutical preparations in the form of tooth paste. Samplees of dental plaque, the msot accused dental deposit which initiates the gingival and periodental diseases, were collected from the teeth surface of ten dental students at one week interval before and after using placebo, followed by two formulae of tooth paste containing 0.1% of quercetrin and naringenin (formulas I and II, respectively). The amount of dental plaque was assessed by the quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The amount of dental plaque was assessed by the Quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The results revealed that most of Gram negative cocci and bacilli were highly affected by the two formulae : the number of actinomycetes were decreased after using formula I and disappeared completely by the sue of formula II, while the number of Gram positive streptococci was highly decreased after the treatment with the two formulae. These results indicate a possible use of flavonoids to inhibit dental plaque formation.

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Some Observations on the Comparison of the Modified Whiteside Test and the California Mastitis Test for the Detection of Bovine Mastitis (젖소의 유방염(乳房炎) 검진(檢診)을 위한 Modified Whiteside Test법(法)과 California Mastitis Test법(法)의 비교시험(比較試驗)에 관(關)하여)

  • Cho, Doo Yeoun;Cheong, Chang Kook
    • Korean Journal of Veterinary Research
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    • v.10 no.1
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    • pp.47-51
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    • 1970
  • The diagnostic effects of the Modified Whiteside Test(M.W.T.) and the California Mastitis Test(C.M.T.) for detection of bovine mastitis were studied using 372 quarter milk samples collected from six dairy herds in suburbs of Suwon city in order to compare the effectiveness of the two methods. The leucocyte count and bacteriological observation were also performed to support analysing the results. The results obtained were as follows; 1. The CMT showed more specificity than MWT for leucocytes in the abnormal milk. 2. When comparison was based on Identical ratings, agreement was obtained in 56.4% of the samples. The stronger reaction had the higher agreement and the CMT was found to be more specific than MWT in detection of the sub-clinical mastitis. 3. The CMT reaction was instantaneous and sufficiently clear-cut. 4. The results of the CMT leucocyte count and bacteriological observation were completely agreed in 69.9% of the samples. When degrees of reaction and the leucocyte count were considered, the CMT showed agreement in 93.5% of the samples.

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Bacteriological Study of Sea Water and Oyster in Charan Bay, Korea (자란만의 해수 및 굴의 세균학적 연구)

  • CHOI Jong-Duck;JEONG Woo-geon;KIM Poong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.3
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    • pp.429-436
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    • 1998
  • A bacteriological study of sea water and oyster in Charan Bay was conducted to evaluate sanitary conditions of the bay and compliance of waters with the recommended bacteriological criteria for the designated area of shellfish cultivation, The Samples were collected at 23 sampling stations(Fig. 1 and Fig. 2) estaslished once a month from January 1997 to December 1997, During the study period, temperature ranged from 4.7 to $25.6^{\circ}C$, transparency ranged from 3.3 to 6.2m chemical oxygen demand ranged from 1.67 to 2.18 mg/$\ell$, dissolved oxygen demand ranged from 5.4 to 10.0 mg/$\ell$ dissolved nitrogen ranged from 1.65 to 7.88 $\mu$g-at/$\ell$, phosphate ranged from 0.15 to 1.16 $\mu$g-at/$\ell$, Chlorophylla-a ranged from 0.95 to 12.69mg/$\ell$. The coliform group and fecal coliform MPN's of sea water were ranged from <1.8$\~$l,600 and <1.8$\~$540, respectively. The coliform group and fecal coliform MPN's of oysters were ranged from <18$\~$16,000 and <18$\~$1,400, respectively. The viable cell counts in oyster ranged from $1.5\times10^2$ to $7.5\times10^3$. The bacteriological criteria of sea water in shellfish growing area should be less than 70 per 100 ml of sea water for median value of coliform MPN, and below $10\%$ of the samples which contain over than 230 for coliform MPN or over than 43 for fecal coliform MPN. The sea water from 432 samples were complied water coliform criteria recommended for designated shellfish growing area. The coliform group, fecal coliform, classification of coliform group with IMViC reactions and pathogenic vibrios were analyzed. During the study period, infectious bacteria such as Vibrio cholerae, Salmonella sp, and Shigella sp, were not detected from the samples, but detection ratios of Vibrio parahaemolyticus and Vibrio vulnifirus were $7\~17\%$ in summer months.

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Evaluation of the Microbiological Safety for the Seawater in a Part of the South Coast, Korea (남해안 일부해역 해수의 세균학적 위생안전성 평가)

  • Park, Yeong Min;Yoon, Hyun Jin;Ham, In Tae;Yoo, Hean Jae;Choi, Jong-Duck
    • Journal of Food Hygiene and Safety
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    • v.30 no.4
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    • pp.350-358
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    • 2015
  • This study was designed to evaluate the sanitary characteristics of sea water in a part of the south coast, Korea and to check the seawater which is in compliance with the recommended bacteriological criteria for shellfish cultivation. The samples of sea water were collected at 14 sampling stations established in the survey area between March 2014 and October 2014. Food poisoning caused by seafood consumption is often associated with pathogenic microorganisms originated from fecal contamination. Therefore, fecal coliform is very important criteria for evaluating the safety of fisheries in coastal areas. The range of geometric mean (GM) and the estimated 90th percentile values of total coliform were 4.1~83.1 MPN/100 mL, and 11.7~834.1 MPN/100 mL, respectively. The GM and the estimated 90th percentile values of fecal coliform were 2.5~22.7 MPN/100 mL and 2.5~170.0 MPN/100 mL, respectively. Therefore, the bacteriological safety of seawater at this shellfish-growing area met with the Korean Shellfish Sanitation Program (KSSP) criteria for a growing area. However, the values are seasonally exceed the KSSP criteria, suggesting that the monitoring and evaluation of seawater quality is very important in shellfish-growing area.