• 식물병과 농업
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• 제5권2호
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• pp.111-114
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• 1999

A bacterial disease of rice plant that rotted the sheath to brown was found in rice plants at Tanbuk Uisong Kyungbuk in June 1999, When the bacterial isolates from the diseased rice plants were inoculated to health plant by the artificial needle prick method the same symptoms were examined. According to its characteristics and pathogenicity on the his plant the causal bacterium was identified as Burkholderia glumae which is known as the pathogen of bacterial grain rot of rice.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 임시총회 및 추계 학술발표회
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• pp.61.2-61
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• 1999

• The Plant Pathology Journal
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• 제29권3호
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• pp.249-259
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• 2013

Burkholderia glumae causes rice grain rot and sheath rot by producing toxoflavin, the expression of which is regulated by quorum sensing (QS). The QS systems of B. glumae rely on N-octanoyl homoserine lactone, synthesized by TofI and its cognate receptor TofR, to activate the genes for toxoflavin biosynthesis and an IclR-type transcriptional regulator gene, qsmR. To understand genome-wide transcriptional profiling of QS signaling, we employed RNAseq of the wild-type B. glumae BGR1 with QS-defective mutant, BGS2 (BGR1 tofI::${\Omega}$) and QS-dependent transcriptional regulator mutant, BGS9 (BGR1 qsmR::${\Omega}$). A comparison of gene expression profiling among the wild-type BGR1 and the two mutants before and after QS onset as well as gene ontology (GO) enrichment analysis from differential expressed genes (DEGs) revealed that genes involved in motility were highly enriched in TofI-dependent DEGs, whereas genes for transport and DNA polymerase were highly enriched in QsmR-dependent DEGs. Further, a combination of pathways with these DEGs and phenotype analysis of mutants pointed to a couple of metabolic processes, which are dependent on QS in B. glumae, that were directly or indirectly related with bacterial motility. The consistency of observed bacterial phenotypes with GOs or metabolic pathways in QS-regulated genes implied that integration RNAseq with GO enrichment or pathways would be useful to study bacterial physiology and phenotypes.

• 식물병연구
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• 제19권3호
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• pp.177-182
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• 2013

Resvestrol has been known to inhibit bacterial and fungal growth in vitro, and can be accumulated in plant to concentrations necessary to inhibit microbial pathogens. Hence, stilbene synthase gene has been used to transform to synthesize resveratrol in heterologous plant species to enhance resistance against pathogens. In the present study, we investigated the antimicrobial activities of resveratrol and piceid to bacterial and fungal pathogens, which causing severe damages to rice plants. In addition, disease resistance was compared between transgenic rice varieties, Iksan 515 and Iksan 526 transformed with stlibene synthase gene and non-transgenic rice varieties, Dongjin and Nampyeong. Minimum inhibitory concentration of resveratrol for Burkolderia glumae was 437.5 ${\mu}M$, and the mycelial growth of Biplaris oryzae was slightly inhibited at concentration of 10 ${\mu}M$. However, other bacterial and fungal pathogens are not inhibited by resveratrol and piceid. The expression of the stilbene synthase gene in Iksan 515 and Iksan 526 did not significantly enhanced resistance against bacterial grain rot, bacterial leaf blight, sheath blight, and leaf blight. This study is the first report on the effect of resveratrol and piceid against pathogens of rice plant, and changes of disease resistance of transgenic rice plants transformed with stilbene synthase gene.

• 식물병연구
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• 제8권1호
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• pp.1-10
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• 2002

2001년 기상의 특징은 3월부터 5월까지 계속된 극심한 가뭄과 생육기간 내내 계속된 고온현상 그리고 별다른 태풍의 내습 없이 9, 10월 수확기 맑은날의 지속으로 요약할 수 있다. 벼는 순조로운 기상 때문에 잎, 이삭도열병, 세균성벼알마름병, 흰잎마름병, 잎집무늬마름병의 발생이 예년에 비해 경미하였으나, 감비추진 정책에 의해 생육후기 깨씨무늬병이 발생하여 전국적으로 문제시되었으며 그동안 발생이 적었던 줄무늬잎마름병이 경기, 충청, 호남 서해안지역을 중심으로 많이 발생하였다. 고추는 생육기 비가 많이 왔던 전남북 지역에서 역병 발생이 심하였으며 토마토와 오이는 대부분 예년에 비해 병발생이 적었다. 수박의 경우는 꽃자리 부분이 썩는 푸른곰팡이병이 전국적으로 발생하였으며 CGMMV는 전년에 비해 상당폭 발생면적이 감소하였고 노지재배의 경우 탄저병 발생이 예년에 비해 심하였다. 참외와 딸기도 전년에 비하여 병발생이 경미하였으며 예년에 발생이 많았던 참외의 급성위조증상도 발생이 대폭 감소하였다. 마늘·양파의 흑색썩음균핵병은 봄철의 가뭄과 고온현상으로 병발생의 전년의 절반수준으로 감소하였다. 감자의 풋마름병은 발병포장이 증가하는 경향이었으며 고구마의 덩굴쪼김병도 발생이 감소하였다. 사과의 경우 전반적으로 병 발생이 예년에 비해 경미하였으며 배는 흰가루병이 많이 발생하였다. 맥류의 붉은곰팡이병은 예년처럼 거기 발생이 없었다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.108.2-109
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• 2003

The Gyeonggi-do Agricultural Research and Extension Services has developed a web-site (www.epilove.com) in collaboration with EPINET to provide information on agricultural weather and rice disease and insect pest management in Gyeonggi-do. Weather information includes near real-time weather data monitored by automated weather stations (AWS) installed at rice paddy fields of 11 Agricultural Technology Centers (ATC) in Gyeonggi-do, and weekly weather forecast by Korea Meteorological Administration (KMA). Map images of hourly air temperature and rainfall are also generated at 309m x 309m resolution using hourly data obtained from AWS installed at 191 locations by KMA. Based on near real-time weather data from 11 ATC, hourly infection risks of rice blast, sheath blight, and bacterial grain rot for individual districts are estimated by disease forecasting models, BLAST, SHBLIGHT, and GRAINROT. Users can diagnose various diseases and insects of rice and find their information in detail by browsing thumbnail images of them. A database on agrochemicals is linked to the system for disease and insect diagnosis to help users search for appropriate agrochemicals to control diseases and insect pests.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1609-1621
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• 2014

The plant pathogen Burkholderia gladioli, which has a broad host range that includes rice and onion, causes bacterial panicle blight and sheath rot. Based on the complete genome sequence of B. gladioli BSR3 isolated from infected rice sheaths, the genome of B. gladioli BSR3 contains the luxI/luxR family of genes. Members of this family encode N-acyl-homoserine lactone (AHL) quorum sensing (QS) signal synthase and the LuxR-family AHL signal receptor, which are similar to B. glumae BGR1. In B. glumae, QS has been shown to play pivotal roles in many bacterial behaviors. In this study, we compared the QS-dependent gene expression between B. gladioli BSR3 and a QS-defective B. gladioli BSR3 mutant in two different culture states (10 and 24 h after incubation, corresponding to an exponential phase and a stationary phase) using RNA sequencing (RNA-seq). RNA-seq analyses including gene ontology and pathway enrichment revealed that the B. gladioli BSR3 QS system regulates genes related to motility, toxin production, and oxalogenesis, which were previously reported in B. glumae. Moreover, the uncharacterized polyketide biosynthesis is activated by QS, which was not detected in B. glumae. Thus, we observed not only common QS-dependent genes between B. glumae BGR1 and B. gladioli BSR3, but also unique QS-dependent genes in B. gladioli BSR3.