• 제목/요약/키워드: bacterial minicell

검색결과 1건 처리시간 0.017초

Generation of Minicells from an Endotoxin-Free Gram-Positive Strain Corynebacterium glutamicum

  • Lee, Jin-Young;Choy, Hyon E.;Lee, Jin-Ho;Kim, Geun-Joong
    • Journal of Microbiology and Biotechnology
    • /
    • 제25권4호
    • /
    • pp.554-558
    • /
    • 2015
  • Drug delivery systems (DDSs) incorporating bacterial minicells have been evaluated as a very powerful tool in view of biocompatibility. However, limited studies have been carried out on these systems, mainly using minicells from Salmonella sp. and Escherichia coli. Thus, we generated a new minicell-producing strain from an endotoxin-free Corynebacterium glutamicum by the inactivation of genes related to cell division. The two knockout strains, ${\Delta}parA$ and ${\Delta}ncgl1366$, showed distinct abilities to produce minicells. The resulting minicells were purified via sequential antibiotic treatments and centrifugations, which resulted in reproducible yields.