• Title/Summary/Keyword: bacterial minicell

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Generation of Minicells from an Endotoxin-Free Gram-Positive Strain Corynebacterium glutamicum

  • Lee, Jin-Young;Choy, Hyon E.;Lee, Jin-Ho;Kim, Geun-Joong
    • Journal of Microbiology and Biotechnology
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    • v.25 no.4
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    • pp.554-558
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    • 2015
  • Drug delivery systems (DDSs) incorporating bacterial minicells have been evaluated as a very powerful tool in view of biocompatibility. However, limited studies have been carried out on these systems, mainly using minicells from Salmonella sp. and Escherichia coli. Thus, we generated a new minicell-producing strain from an endotoxin-free Corynebacterium glutamicum by the inactivation of genes related to cell division. The two knockout strains, ${\Delta}parA$ and ${\Delta}ncgl1366$, showed distinct abilities to produce minicells. The resulting minicells were purified via sequential antibiotic treatments and centrifugations, which resulted in reproducible yields.