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Effects of SIS/PLGA Porous Scaffolds and Muscle-Derived Stem Cell on the Formation of Tissue Engineered Bone (SIS/PLGA 담체와 근육유래 줄기세포를 이용한 생체조직공학적 골재생)

  • Kim Soon Hee;Yun Sun Jung;Jang Ji Wook;Kim Moon Suk;Khang Gilson;Lee Hai Bang
    • Polymer(Korea)
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    • v.30 no.1
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    • pp.14-21
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    • 2006
  • Tissue engineering techniques require the use of a porous biodegradable/bioresorbable scaffold, which server as a three-dimensional template for initial cell attachment and subsequent tissue formation in both in vitro and in vivo. Small intestinal submucosa (SIS) has been investigated as a source of collagenous tissue with the potential to be used as biomaterials because of its inherent strength and biocompatibility. SIS-loaded poly(L-lactide-co-glicolide)(PLGA) scaffolds were prepared by solvent casting/particle leaching. Characterizations of SIS/PLGA scaffold were carried out by SEM, mercury porosimeter, and so on. Muscle-derived stem cells can be differentiated in culture into osteoblasts, chondrocytes, and even myoblasts by the controlling the culture environment. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide(MTT) test. Osteogenic differential cells were analyzed by alkaline phosphatase(ALP) activity. SIS/PLGA scaffolds were implanted into the back of athymic nude mouse to observe the effect of SIS on the osteoinduction compared with controlled PLGA scaffolds. Thin sections were cut from paraffin embedded tissues and histological sections were conducted hematoxylin and eosin (H&E), Trichrome, and von Kossa. We observed that bone formatioin of SIS/PLGA hybrid scaffold as natural/synthetic scaffold was better thean that of only PLGA scaffold. It canb be explained that SIS contains various kinds of bioactive molecules for osteoinduction.

Analysis of Terrain Change Caused by Mining Development using GIS (GIS를 이용한 광산개발지역의 추이 현황 분석)

  • Lee Hyung-Seok
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.24 no.3
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    • pp.261-269
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    • 2006
  • There is a need to restore the terrain back its natural environment after mining development. It is necessary to compare the original and developing surfaces for post-management and to analyze the terrain change to develop a process for efficient restoration plan. This study analyzes and compares change to the terrain by annual mining development using GIS. Contours digitized with CAD based on photogrammetry are classified into annual data and created by Triangulated Irregular Network (TIN). By producing profiles and cross sections using TIN, many stations are distinguished. As a result of the terrain changes caused by mining development from 2000 to 2003 by operating elevation values each cell converted to raster from TIN, $11,094,460m^3$ are cut and $5,127,968m^3$ are filled up to 46% of cut volume, and annual surface changes of cut and fill area to mining are analyzed to visual and quantitative data. This study is used for the restoration plan and additional mining. And it is expected that this annual change, caused by mining development, can be used to return the terrain close to its original condition for finished mining area.

Development of Smart Multi-function Ground Resistivity Measuring Device using Arduino in Wind Farm (풍력 발전단지내 아두이노를 활용한 스마트 다기능 대지 고유 저항 측정 장치 개발)

  • Kim, Hong-Yong;Yoon, Dong-Gi;Shin, Seung-Jung
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.19 no.6
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    • pp.65-71
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    • 2019
  • Conventional methods of measuring ground resistance and ground resistance field measurement are used to measure voltage drop according to the resistance value of the site by applying current by installing a constant interval of measurement electrode. If the stratified structure of the site site is unique, errors in boundary conditions will occur in the event of back acid and the analysis of the critical ground resistance in the ground design will show much difference from simulation. This study utilizes the Arduino module and smart ground measurement technology in the convergent information and communication environment to develop a reliable smart land resistance measuring device even if the top layer of land is unique, to analyze the land resistance and accumulate data to predict the change in the age of the land. Considering the topographical characteristics of the site, we propose a ground resistance measuring device and its method of measuring ground resistance so that the auxiliary electrode can be installed by correctly positioning the angle and distance in measuring ground resistance. Not only is ground resistance value obtained through electrodes installed to allow accurate ground resistance values to be selected, but it can also be used as a useful material for installing electrical facilities in similar areas. Moreover, by utilizing reliable data and analyzing the large sections of the site, a precise analysis of the site, which is important in ground design as well as construction cost, is expected to be used much in ground facility design such as potential rise.

The Effects of Complex Motor Training on Motor Function and Synaptic Plasticity After Neonatal Binge-like Alcohol Exposure in Rats (복합운동훈련이 신생 흰쥐의 알코올성 소뇌손상 후 운동기능 및 신경연접가소성에 미치는 영향)

  • Lee, Sun-Min;Koo, Hyun-Mo;Kwon, Hyuk-Cheol
    • Physical Therapy Korea
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    • v.12 no.3
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    • pp.56-66
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    • 2005
  • The purposes of this study were to test that complex motor training enhance motor function significantly, to test change in cerebellum, and to test the synaptic plasticity into the immunohistochemistry response of synaptophysin. Using an animal model of fetal alcohol syndrome - which equates peak blood alcohol concentrations across developmental period - the effects of alcohol on body weight during periods were examined. The effect of complex motor training on motor function and synaptic plasticity of rat exposed alcohol on postnatal days 4 through 10 were studied. Newborn rats were assigned to one of two groups: (1) normal group (NG), via artificial rearing to milk formula and (2) alcohol groups (AG), via 4.5 g/kg/day of ethanol in a milk solution. After completion of the treatments, the pups were fostered back to lactating dams, where they were raised in standard cages (two-and three animals per cage) until they were postnatal 48 days. Rats from alcohol group of postnatal treatment then spent 10 days in one of two groups: Alcohol-experimental group was had got complex motor training (learning traverse a set of 6 elevated obstacles) for 4 weeks. The alcohol-control group was not trained. Before consider replacing with "the experiment/study", (avoid using "got" in writing) the rats were examined during four behavioral tests and their body weights were measured, then their coronal sections were processed in rabbit polyclonal antibody synaptophysin. The synaptophysin expression in the cerebellar cortex was investigated using a light microscope. The results of this study were as follows: 1. The alcohol groups contained significantly higher alcohol concentrations than the normal group. 2. The alcohol groups had significantly lower body weights than the normal group. 3. In alcohol groups performed significantly lower than the normal group on the motor behavioral test. 4. In alcohol-control group showed significantly decreased immunohistochemistric response of the synaptophysin in the cerebellar cortex compared to the nomal group. These results suggest that improved motor function induced by complex motor training after postnatal exposure is associated with dynamically altered expression of synaptophysin in cerebellar cortex and that is related with synaptic plasticity. Also, these data can potentially serve as a model for therapeutic intervention.

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A Textile Analysis of Woolen Tapestry Curtain in Seoul Museum of Craft Art (서울공예박물관 소장 모담방장(毛毯房帳) 직물 분석)

  • An, Boyeon;Lee, Jangjon;Lee, Ryangmi
    • Journal of Conservation Science
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    • v.35 no.6
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    • pp.612-620
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    • 2019
  • A woolen tapestry curtain, owned by the Seoul Museum of Craft Art, is composed of tabby by cotton-warp and wool-weft threads and its patterned part is shown as a tapestry; paint with pigment has been added to it. The chromaticity of this curtain was measured and the substances in the deep red color were confirmed as Hg by an analysis of the ingredients through X-ray fluorescence. This is presumed to be cinnabar or vermilion. Analyses were performed on a total of seven fabric samples, including the warp & weft of the fabric, its trimming, and its back fabric. As a result, the warp of the woolen tapestry curtain was determined to be a cotton fiber with a middle hole or lumen in the cross-section. Furthermore, an infrared peak likewise showed O-H and C-O binding. Wool fibers as wefts were identified with circular and oval cross-sections and IR peaks showed N-H/O-H stretching and amide(-CONH-). The animal hair samples used in the wool fiber are believed to have come from long-tailed goral or goats and the possibility of using easy-to-spin sapsal dog hair is also not to be overlooked. This was determined through a contrast analysis by the Cultural Heritage Administration to identify the animal species used in the tapestry.

A study on the Changes in form and spatial uses of Urban Hanok in Bukchon, Seoul (서울 북촌한옥의 변화양상에 관한 연구 - 북촌 가꾸기 사업에 따른 2002~2007 한옥 대수선 사례를 대상으로 -)

  • Song, In-Ho;Kim, Young-Soo;Cho, Eun-Joo
    • Journal of architectural history
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    • v.18 no.2
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    • pp.47-63
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    • 2009
  • This study focused on changes in form and spatial uses of Urban Hanok in Bukchon, Seoul. There are 10 representative cases which have been renovated through the policy of 'Preservation & Regeneration of Bukchon' by Seoul metropolitan government and other experts. Changes in form and spatial uses of Urban Hanok in Buckon are as follows. First, Changes of scale. Trough removing extension parts, facade of renovated Hanoks are 'transformed' into recovering their identity. Using basements or lofts, intensive application of spaces is transformation which promotes the vitality of Hanoks. Second, changes of space organization. As Hanok changes its function from residence to commercial or cultural use, il a1so changes space character or reorganizes space organization. It is important that deciding function of Hanok has to adjust its scale and organization. Third, changes of construction performance. Through introducing new material and constructing method, performance of wall has been changed respecting its wooden structure and interior-exterior figure. However, technical studies must back it up not to destroy its value of eco-friendly architecture. Fourth, changes of facility systems, like floor heating system. They changes floor level of Hanok equally, and then sections of Hanok have became simple. Furthermore, inserting new facility space, such as boiler room, stand-up kitchen, bathroom and toilet, organization of space also changed. It is necessary that wise alternative proposal through the method of transformation or mutation must be presented. These four changes can be classification into method of 'transformation' and 'mutation'. Changes of scale are method of transformation and changes of space organization are method of mutation. Also, while changes of construction performance are mutation, changes of facility systems are transformation. Recently, as price of lots have been increased, a lot of Hanoks have been commercialized. Thc commercial energy threat 'the identity of Bukchon as residential area'. From now on, to operate 'identity' and 'vitality' complementary, it is necessary to make up for the preservation policy of Hanok and consolidate renovating standards of Hanok which correspond to character of particular region and building usage.

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Validation of Permanent Deformation Model for Flexible Pavement using Accelerated Pavement Testing (포장가속시험을 이용한 소성변형예측 모델의 검증)

  • Choi, Jeong Hoon;Seo, Youngguk;Suh, Young Chan
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.4D
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    • pp.491-497
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    • 2009
  • This paper presents the results of accelerated pavement tests (APT) that simulate permanent deformation (rutting) of asphalt concrete pavements under different temperatures and loading courses. Also, finite element (FE) analysis has been conducted to predict the test results. Test section for APT is the same as one of test sections at Korea Expressway Corporation test road and is subjected to a constant moving dual tire wheel load of APT at three different temperatures: 30, 40, $50^{\circ}C$. The moving wheel is applied at different loading courses within a 75cm wide wheel path to account for traffic wandering. Also, the effect of wandering on permanent deformation development is investigated numerically with three wandering schemes. In this study, ABAQUS is adopted to model APT pavement section with plain stain elements and creep strain rate model is used to take into account viscoplastic stain of asphalt concrete mixtures, and elastic layer properties are back-calculated from FWD measurements. Plus, the effect of boundary condition and subgrade on FE permanent deformation predictions is investigated. A full FE model that accounted for subgrade provided more realistic rut depth predictions, indicating subgrade has contributed to surface rutting.

A Study of Improvement of Urban Pavement Maintenance Technique based on Pavement Condition Evaluation and FWD Data (도로포장 표면조사와 FWD정보에 기반한 도심지 도로포장 유지보수 기법 개선방안 연구)

  • Lee, Sangyum
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.12
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    • pp.532-541
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    • 2016
  • The objective of this paper is to support accurate pavement condition assessment and decision of proper maintenance method and time by conducting visual inspection and calculating the remaining life of pavement from falling weight deflectometer(FWD) data. Each was implemented in the same long-term performance pavement(LTPP) sections. Visual inspection was executed to measure pavement condition indices such as crack, rutting and international roughness index(IRI) and the Seoul Pavement Index(SPI) was calculated based on these results. The dynamic modulus was back-calculated from the FWD data. The remaining pavement lives were determined from equivalent single axle loading(ESAL) and FWD data. Correlation of maintenance priority by each result value was examined. Consequently, the correlation between remaining life to Crack and Rutting was higher than the other factors or indicesbecause IRI is not related to FWD value and SPI value consists with IRI value and other indices. The R-square value of correlation of FWD with Crack and Rutting was 0.65, which indicated an insufficient correlation. Consequently, when decision of maintenance of method, time, etc. is determined, FWD data have to be considered with Crack and Rutting because of those relations.

A Study on the Residual Current in the Cheju Strait (제주해협의 해수유동특성에 관한 연구)

  • KIM Sang Hyun;RHO Hong Kil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.5
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    • pp.759-770
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    • 1997
  • The general flow patterns in the Cheju Strait have been investicated by analyzing the current observations measured in $1986\~1989$ by current meter mooring in 3 north-south sections in the Cheju Strait and at 4 observation points around Cheju Harbour, and measured in $1981\~1987$ by drogue tracking. 1. In the Cheju Strait, there are eastward or northeastward residual currents, which implies that sea waters flow into through the whole western section and flow out through the whole eastern section in the Cheju Strait. The velocity of residual currents are $5.2\~30\;cm/sec$ in 10 m layer and $1.3\~24cm/sec$ in mid-bottom layer. Generally, the flow is strong along the deepest through and the northern part, and weak in the shallow areas near Chuja Islands and Bogil Island. 2. In the western entrance of the Cheju Strait, the observed mean residual velocity is 6.93 cm/sec and the volume transport is 0.384 Sv. There are a big discrepancy between the observed residual currents and the geostrophic currents. 3. Near the frontal areas northwest to Chuja Islands, warm and saline offshore waters, flow northward about 5 miles into the southern coastal areas of the Korean Peninsula in flood, and flow back rather eastward or southeastward than southward in ebb. So, warm and saline waters flow along coastal areas, being mixed with coastal waters. As a result, the northwestern area of Chuja Islands plays a role of the entrance of influx of warm and saline offshore water to the southwestern coastal areas of the Korean Peninsula. It should be stressed that this flow pattern is not due to the residual flows, but to the temporal (tidal) flows.

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Tissue response of Pro-Root® MTA with rhBMP-2 in pulpotomized rat teeth (백서에서 치수 절단술 시행 시 Pro-Root® MTA 단독 사용군과 rhBMP-2 혼용 사용군 간의 조직 반응 비교 연구)

  • Park, Kyung-Tae;Yang, Won-Kyung;Ko, Hyun-Jung;Kim, Mi-Ri
    • Restorative Dentistry and Endodontics
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    • v.32 no.5
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    • pp.403-410
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    • 2007
  • The purpose of this study was to investigate whether rhBMP-2 (BMP2) could induce synergistic effect with $Pro-Root^{(R)}$ MTA (MTA) in pulpotomized teeth in the rats. Healthy upper first molars from thirty-two, 10 weeks old, Sprague-Dawley rats were used for this investigation. The molars were exposed with round bur, and light pressure was applied with sterilized cotton to control hemorrhage. 1.2 grams of MTA cement was placed in right first molars as a control group. In left first molars, $1\;{\mu}g$ of BMP2 was additionally placed on exposed pulps with MTA. All cavities were back-filled with light-cured glass-ionomer cements. The rats were sacrificed after 2 weeks and 7 weeks, respectively. Then histologic sections were made and assessed by light microscopy. Data were statistically analyzed via student t-test with SPSSWIN 12.0 program (p < 0.05). Inflammation observed in 2 weeks groups were severe compared to the 7 weeks groups. But the differences were not statistically significant. BMP2-addition groups had less inflammation than MTA groups in both periods, though these differences were also not statistically significant. In conclusion, the combination of BMP2 and MTA showed no differences with MTA only for pulpotomy of rat teeth.