• 제목/요약/키워드: back-analyses

검색결과 390건 처리시간 0.024초

Functional Analysis of a Histidine Auxotrophic Mutation in Gibberella zeae

  • Seo, Back-Won;Kim, Hee-Kyoung;Lee, Yin-Won;Yun, Sung-Hwan
    • The Plant Pathology Journal
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    • 제23권2호
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    • pp.51-56
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    • 2007
  • A plant pathogenic fungus, Gibberella zeae (anamorph: Fusarium graminearum), not only generates economic losses by causing disease on cereal grains, but also leads to severe toxicosis in human and animals through the production of mycotoxins in infected plants. Here, we characterized a histidine auxotrophic mutant of G. zeae, designated Z43R1092, which was generated using a restriction enzyme-mediated integration (REMI) procedure. The mutant exhibited pleiotropic phenotypic changes, including a reduction in mycelial growth and virulence and loss of sexual reproduction. Outcrossing analysis confirmed that the histidine auxotrophy is linked to the insertional vector in Z43R1092. Molecular analysis showed that the histidine requirement of Z43R1092 is caused by a disruption of an open reading frame, designated GzHIS7. The deduced product of GzHIS7 encodes a putative enzyme with an N-terminal glutamine amidotransferase and a C-terminal cyclase domain, similar to the Saccharomyces cerevisiae HIS7 required for histidine biosynthesis. The subsequent gene deletion and complementation analyses confirmed the functions of GzHIS7 in G. zeae. This is the first report of the molecular characterization of histidine auxotrophy in G. zeae, and our results demonstrate that correct histidine biosynthesis is essential for virulence, as well as sexual development, in G. zeae. In addition, our results could provide a G. zeae histidine auxotroph as a recipient strain for genetic transformation using this new selectable marker.

뇌파의 한의학적 진단 지표로의 활용 방안에 대한 연구초안 (The methodology on the application of EEG as a diagonostic measures in Korean Traditional Medicine)

  • 서영효;김경철;김보경
    • 동의신경정신과학회지
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    • 제18권1호
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    • pp.37-61
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    • 2007
  • Objective : By examining EEG status in Korean Traditional Medicine (KTM) from the viewpoint of 'form-qi theory(形氣論)', We wish to prepare for the fundamentals of applicability of KTM diagnoses to EEG. In addition, through reinterpretation of existing Western Medicine reports from the viewpoint of KTM, We tried to find out interrelationship between them. Method : In this paper, a methodology applicable to KTM diagnoses of EEG is presented from the EEG features in waveform characteristics, personalized diversity, and cognitive activity reflection. Results : Frequency bands are assigned to corresponding one of the eight trigrams in terms of yin/yang balance, which is analogous with EEG spectrum analysis mostly used in EEG quantification. The amplitude ratio of each EEG for each frequency band gives meaningful index numbers which can be used in EEG data interpretation, and every index number is named after the sixty four hexagrams. These approaches are adopted through both '4-band classification system and '6-band classification system', and applied to pre-existing reported EEG data obtained from normal adults. These analyses show that changes and distribution pattern in the index numbers are observed as a whole on both left-right line and front-back line connecting EEG measurement cephalic electrodes. And differences in distribution pattern of three index numbers deduced from '6-band classification system' are discussed according to constitution. Conclusion : The index numbers introduced here, which are the spectral power ratio for each EEG, are based on KTM yin/yang balance. These index numbers vary according to cephalic location, so its application in terms of traditional meridian theory is strongly expected. The index number distribution also shows different patterns according to constitution.

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구급 빅데이터의 분석과 활용 방안에 관한 연구 (Analysis and utilization of emergency big data)

  • 이성연;권유진;임동오;김민규;박희진;권혜란;주영철
    • 한국응급구조학회지
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    • 제20권1호
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    • pp.41-55
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    • 2016
  • Emergency statistics for cities and provinces are currently derived using simple results of comparative numerical data, but there is a limit to the ability to analyze and compare deviations relevant to a specific city and province. This study aims to derive various correlations through statistical analysis of emergency and rescue data for Gwangju Metropolitan City and to develop an analytical model that can be applied nationwide. With the new statistical model, further detailed analysis is possible beyond simple evaluation of rescue data, through links to other institutions and analyses using keywords from Internet portal sites and social networks. Second, a system which that can analyze data that are not shared is required. Through this system, a large amount of data can be automatically analysed in real time. Third, the results should flow back for application in various policies. A real-time monitoring and management system should be created for abnormal patterns of disease. In addition, the results should be available to tailor services for individuals, communities, or specific organizations.

대학 학생 식당의 위생적 공간 계획에 관한 연구 - 서울 E 대학 학생 식당 고객 공간에 HACCP 적용 중심으로 - (A Study on Hygienic Space Planing of a University Student Restaurant - Focused on the customer space in E University Student Restaurant in Seoul Applying HACCP -)

  • 이종란
    • 한국실내디자인학회논문집
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    • 제20권3호
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    • pp.182-189
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    • 2011
  • This research suggests a way of hygienic space planing of the customer space in the university student restaurant of E University in Seoul. For this study. the behavior observation in the restaurant was conducted in order to analyze the sequence of customer behavior related space planing applying HACCP (Hazard Analysis And Critical Control Point). Also the survey was conducted to analyze opinions of customers about hygiene of the space. The results of the analyses of the observation and the survey were similar in terms of hygiene. In conclusion, for hygienic space planing of the customer space in restaurant, the space circulation should be planned according to the hygienic sequence of customer behavior. The spaces in restaurant should be divided into two areas: the clean area and the contaminated area to prevent cross-contamination. The clean area, such as spaces preparing dishes and food including water and table hall where customers eat, should be separated from the contaminated area such as the spaces where waste is handled and where dirty dishes are kept. In order to prevent cross-contamination, hygiene facility to remove contamination should be located before going to the clean area. More specifically, the hygiene facility should be located between a cash counter and a space preparing dishes so that customers can wash their hands before they touch dishes. Space arrangement with feed-back process to check the possible points of cross-contamination and correct space arrangement until the possibility of cross-contamination is removed in the restaurant.

반사파 흡수용 신 조파제어 시스템의 설계 (A New Wavemaker Control System with Reflected Wave Absorbing Capability)

  • 전인식;박우선;오영민
    • 한국해안해양공학회지
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    • 제5권4호
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    • pp.329-337
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    • 1993
  • 파낭수조를 이용하는 수리실험에서 실험결과들 사이에 동질성 및 호환성을 유지하는 것은 매우 중요한 문제이며 이는 목적파낭을 얼마나 장시간 수조내에 유지시킬 수 있는가에 달려있다. 본고에서는 계측파랑을 목적파랑에 일치시키기 위한 순환성 제어회로를 구성하고 회로해석을 통하여 컴퓨터로 용이하게 조파기 하드웨어를 제어할 수 있는 알고리즘을 제시하였다. 알고리즘의 주된 기능으로서 시간영역에서 이산적으로 적용되는 반사파 흡수필터를 포함하였다. 필터의 구현방식은 이산적 입력과 출력이 직접대응하는 방식이므로 데이타의 통계적 성격이 사전에 알려져 있지 않은 불확정적 입력데이타에 대해서도 적용이 가능하다. 예제해석을 통하여 알고리즘을 수치시험해 분 결과, 임의의 규칙 또는 불규칙 반사파 시그널에 대해서 본 제어회로의 반사파 흡수기능이 매우 탁월함을 입증하였다.

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컴퓨터 시뮬레이션(CAE)을 이용한 자동차용 엔진 크레들의 하이드로-포밍 공정 연구 (Hydro-forming Process of Automotive Engine Cradle by Computer Aided Engineering (CAE))

  • 김기주;최병익;성창원
    • 한국자동차공학회논문집
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    • 제16권1호
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    • pp.86-92
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    • 2008
  • Recently, the use of tubes in the manufacturing of the automobile parts has increased and therefore many automotive manufactures have tried to use hydro-forming technology. The hydro-forming technology may cause many advantages to automotive applications in terms of better structural integrity of the parts, lower cost from fewer part count, material saving, weight reduction, lower spring-back, improved strength and durability and design flexibility. In this study, the whole process of front engine cradle (or front sub-frame) parts development by tube hydro-forming using steel material having tensile strength of 440MPa grade is presented. At the part design stage, it requires feasibility study and process design aided by CAE (Computer Aided Design) to confirm hydro-formability in details. Effects of parameters such as internal pressure, axial feeding and geometry shape on automotive sub-frame by hydro-forming process were carefully investigated. Overall possibility of hydro-formable sub-frame parts could be examined by cross sectional analyses. Moreover, it is essential to ensure the formability of tube material on every forming step such as pre-bending, preforming and hydro-forming. At the die design stage, all the components of prototyping tools are designed and interference with press is examined from the point of geometry and thinning.

설계변수에 따른 알루미늄 범퍼 시스템의 저속 충돌해석 (Low Speed Crash Behaviour of Aluminium Bumper System W.R.T. Design Variables)

  • 김대영;한보석;홍민선;김동옥;전성식
    • 한국자동차공학회논문집
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    • 제25권1호
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    • pp.11-18
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    • 2017
  • In the present study, the low speed (4 km/h) crash behaviour of an aluminium bumper system was characterised by FE analyses based on the FMVSS 581, which regulates automotive bumpers. Two types of cross-sectional designs, i.e., Model 1, which contains a single rib and Model 2, double ribs, have been considered along with Al7021, 6082 and 6060 for the aluminium bumper back beam. Variations in thickness starting from 2 to 4 mm of the bumper system cross-section in the FE model was implemented in order to investigate the thickness effect on the bumper's crash behaviour.. Three kinds of design variables, namely, number of ribs, material and thickness, are considered. The FE analysis results are summarised with the maximum load and the Specific Energy Absorption (SEA) since they are the key factors in determining the crashworthiness of automotive structures. The results may also be able to indicate how to achieve lightweight structure of the automotive bumper system either directly or indirectly.

질량중심 및 압력중심을 이용한 여자 중학생의 하이클리어 동작 비교 분석 (Comparative analysis of high-clear motion of female middle school student using COM and COP parameter)

  • 한기훈;박준성;권우문;김재웅
    • 한국응용과학기술학회지
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    • 제37권4호
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    • pp.786-793
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    • 2020
  • 본 연구의 목적은 배드민턴 여자 중학생 전문선수(10명)와 방과 후 배드민턴 수업에 참여하는 일반학생(10명)의 하이클리어 동작 시 COM과 COP의 수평거리, 그리고 COM과 COP의 이동패턴의 차이를 비교 분석하는데 있다. 두 그룹 간 하이클리어 스트로크 이후 착지 시점(E4)에서 COM과 COP의 X축과 Y축에서의 수평거리와 백스윙, 포워드스윙, 스윙전체의 X축, Y축, 그리고 Z축에서 COM의 최대이동범위의 차이를 비교하기 위해 독립표본 T-test를 실시한 결과, 첫째, 하이클리어 스트로크 임팩트 이후 착지 시점(E4)에서 여자 중학생 전문선수는 전후방향(X축)에서 COM와 COP지점이 일반학생에 비해 매우 가깝게 위치하였다. 둘째, 전문선수는 COM의 수직움직임, 일반선수는 COM의 수평움직임을 적극적으로 활용하였다.

초음속 이젝터 펌프 유동에 관한 수치해석 (A CFD Study of the Supersonic Ejector-Pump Flows)

  • 이영기;김희동;서태원
    • 한국추진공학회지
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    • 제3권4호
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    • pp.58-66
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    • 1999
  • 초음속 이젝터 유동특성은 압축성, 비정상성, 충격파 둥으로 인하여 충분히 알려져 있지 않다. 종래의 이론적/실험적 연구결과들은 대부분 1차원 가정 하에서 얻어진 것들이며, 현재까지 수치계산법을 이용하여 이젝터 내부 유동장을 해석한 실례가 많지 않다. 뿐만 아니라 기존의 수치계산 결과들은 2차유동의 질량유량이 매우 작거나 없는 단순한 유동장에 대한 것들이었다. 본 연구에서는 초음속 이젝터-펌프 유동장을 수치적으로 해석하기 위하여 축대칭 수치계산 모델을 이용하였다. 수치계산은 축대칭 압축성 Navier-Stokes 방정식에 유한체적법을 적용하였으며, 표준형 $\kappa$ -$\varepsilon$ 난류모델을 이용하였고, 2차유동의 질량유량을 변화시켜 1차유동과 2차유동의 질량비의 변화에 따른 이젝터 내부 유동장의 변화를 조사하였다. 그 결과, 2차목을 가지지 않는 일정 단면적의 혼합부를 가지는 이젝터의 경우, 이젝터 내부의 유동장 특성은 2차유동의 질량유량의 변화와 거의 무관하게 나타났다. 그러나 2차목을 가지는 경우는 2차유동의 질량유량의 증가에 따른 노즐출구 주위에서 배압으로 인하여 이젝터 내부 유동장이 크게 변화하였다.

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니켈기 초내열합금 분말의 고용화 열처리 후 냉각속도에 따른 크리프특성 분석 (Analyses of Creep Properties of Ni-base Superalloy Powders as Cooling Rate after Solid Solution Heat Treatment)

  • 전찬;이영선;배병범;김홍규;홍성석;김동훈;윤존도;윤은유
    • 한국분말재료학회지
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    • 제23권3호
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    • pp.247-253
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    • 2016
  • In this study, solid solution heat treatment of consolidated nickel-based superalloy powders is carried out by hot isotactic pressing. The effects of the cooling rate of salt quenching, and air cooling on the microstructures and the mechanical properties of the specimens are analyzed. The specimen that is air cooled shows the formation of serrated grain boundaries due to their obstruction by the carbide particles. Moreover, the specimen that is salt quenched shows higher strength than the one that is air cooled due to the presence of fine and close-packed tertiary gamma prime phase. The tensile elongation at high temperatures improves due to the presence of grain boundary serrations in the specimen that is air cooled. On the contrary, the specimen that is salt quenched and consists of unserrated grain boundaries shows better creep properties than the air cooled specimen with the serrated grain boundaries, due to the negative creep phenomenon.