• Journal of Photoscience
• /
• v.9 no.2
• /
• pp.269-271
• /
• 2002

To investigate the visual and extra-ocular photoreception, we cloned the opsin genes in ayu (Plecoglossus allivelis). Amplified fragments encoding exon-4 (-5) of opsin cDNAs were cloned from the retina and brains of ayu, and sequenced. One clone was identified as rod (AYU-Rh), two as green cone (AYU-GI, -G2), one as red cone (A YU-R), two as ultraviolet cone (AYU-UVl, UV2), one as VA (AYU-VA), and one as extra-ocular rod (AYU-ExoRh) opsins. 335 amino acids sequence deduced from the full-length cDNA of AYU-Rh showed high identity with that of other fish. Southern blotting analysis indicated that ayu possess two 'rhodopsin' genes, one is visual rhodopsin and the other is non-visual extra-ocular rhodopsin. In situ hybridization showed that the mRNA of AYU-Rh was localized only in rod cells in the retina. On the other hands, AYU-ExoRh was expressed only in the pineal. We cloned two isoforms (AYU-VAM and -VAL) of VA opsin from ayu. The deduced amino acid sequences of these variants were identical to each other within the first 342 residues, but they showed divergence in the C-terminal sequence. AYU- VAL corresponded to the long isoform found in other fish, and AYU-VAM was identified as a new type of VA opsin variant. Pal-VAM is a new probably functional non-visual photoreceptive molecule in fish.

• Korean Journal of Veterinary Research
• /
• v.58 no.1
• /
• pp.39-43
• /
• 2018

The efficacy of using a bacteriophage (phage) to control Flavobacterium psychrophilum (F. psychrophilum) infection of ayu (Plecoglossus altivelis altivelis) was evaluated in this study. Intramuscular challenge failed to induce sufficient infection levels; therefore, a newly designed net-scratch challenge method was also used to induce bacterial infection. Administration of phage PFpW-3 in F. psychrophilum-infected ayu showed notable protective effects, increased survival rates and mean times to death. Additionally, the fate of inoculated bacteria and phage in ayu were investigated. Our results suggest that the phage PFpW-3 could be considered an alternative biocontrol agent against F. psychrophilum infections in ayu culture.

• The Southeast Asian review
• /
• v.22 no.2
• /
• pp.171-199
• /
• 2012

Saman, a novel written by Ayu Utami, has been recognized as the symbol of the politico-social changes, which began to occur since the collapse of Suharto' New Order regime in 1998. In the novel, Ayu Utami showed the spirits of resistance against various absurd socio-political circumstances during the New Order era such as pressure on discussion, abuse of power, politics-business collusion, patriarchism, and suppression of gender. In representing those spirits, Ayu Utami used unconventional structure-making, fresh feedback and multilayered descriptions of the figures, which brought her a fame as the pioneer of the Fragrant Literature (Angkatan Wangi or chick-lit). Ayu Utami particularly criticized that, under the name of sustaining the national integrity and identity, the New Order regime enhanced patriarchal system, which consequently infringed gender equality and women's rights to self-determination. In addition, Ayu Utami argued that the abuse of power and politics-business collusion, which were prevalent during the New Order period, destroyed lives of the masses and the Indonesian society.

• Ocean and Polar Research
• /
• v.24 no.3
• /
• pp.179-187
• /
• 2002

The Ayu Trough, located in the southern end of the Philippine Sea, represents a divergent boundary between the Philippine Sea and the Caroline Plates. A detailed geophysical survey was carried out in the Ayu Trough by R/V Onnuri. Topographically, the Ayu Trough resembles an slow spreading ridge. The trough can be divided into three sections: the south $(0^{\circ}-1^{\circ}30'N),\;middle\;(1^{\circ}30'-4^{\circ}N)$, and north $(4^{\circ}-6^{\circ}30'N)$. The seafloor in the middle section is characterized by features asymmetric with respect to the axis. These features were probably produced by NW-SE and NNW-SSE extensions and seem to support the argument that the opening of the Ayu Trough occurred in an oblique fashion. Farther south, a long transform fault but with a short offset defines the boundary between middle and southern sections. The axial depth increases a stepwise to the south of $1^{\circ}30'N$. A clear difference can be seen between the southern and middle sections with the latter exhibiting much higher mantle Bouguer anomaly values in the axial region. The anomaly indicates that the axial crust perhaps experienced a much higher degree of extension in the middle than in the southern section. The analyses of magnetic field data reveal that the region beyond 100km exhibits considerable variations, whereas the magnetic anomalies within 100km from the trough axis are very much subdued. This observation suggests that the opening of the Ayu Trough involved an initial stage of rifting of existing volcanic arcs, followed by production of new seafloor.

• Ocean and Polar Research
• /
• v.24 no.3
• /
• pp.189-196
• /
• 2002

A multichannel seismic survey was conducted in the southern Ayu Trough which is the only spreading boundary between the Philippine Sea and Caroline plates. The seismic system used in this study comprises of 2.46-l sleeve gun and a 12-channel streamer with a group interval of 6.25m. Migration technique was used to analyze seismic velocity, and poststack depth migration was applied to the stacked data. The sediment thickness obtained from the depth section tends to increase with distance from the spreading axis. Sedimentation rates are poorly constrainted in the study area. The apparent half-spreading rates estimated from the sediment thickness and sedimentation rate from DSDP hole on the caroline plate are 4.7mm/yr and 7.9mm/yr at $1^{\circ}24'N\;and\;0^{\circ}42'N$, respectively, which are fester than Previously suggested. On the basis of new oblique spreading geometry, the recalculated spreading rates are 5.4mm/yr and 9.1mm/yr at $1^{\circ}24'N\;and\;0^{\circ}42'N$, respectively. Seismic sections show that the topography is asymmetric across the Ayu Trough and the acoustic basement is rough. These features are consistent with the earlier suggestion that the Ayu Trough is a slow-spreading divergent boundary. A detailed examination of seismic profiles away from the axis shows that sediments can be divided into two layers which implies a possible change in the spreading rate anuor sedimentation condition during the formation of the trough.

• Fisheries and Aquatic Sciences
• /
• v.14 no.4
• /
• pp.295-301
• /
• 2011

We investigated the genetic structure of Korean and Japanese ayu Plecoglossus altivelis populations by examining 669 individuals from 14 populations using three microsatellite loci. Genetic variation did not differ significantly among the populations examined in terms of allelic number and heterozygosity. Korean populations were genetically close to each other, implying that persistent gene flow has occurred in these populations. This suggests that eastern populations in Korea form a single large population and all of the Korean populations are distinct from the Japanese populations. Pairwise population $F_{ST}$ estimates, principal component analyses, and a neighbor-joining tree showed that genetic separation between the southern and pooled eastern coast populations was probably influenced by restricted gene flow. Hierarchical analysis of molecular variance (AMOVA) revealed a weak but significant genetic structure among three ayu groups (eastern and southern coasts of Korea and the Japan coast), and no genetic variation within groups. The estimated genetic population structure and potential applications of microsatellite markers may aid in the proper management of ayu populations.

• Fisheries and Aquatic Sciences
• /
• v.5 no.3
• /
• pp.172-177
• /
• 2002

This study was conducted to investigate the utilization of Sargassum meal in the diet on juvenile ayu (Plecoglossus altivelis) reared in seawater. White fish meal and wheat flour were used as the dietary protein and carbohydrate sources in the control diet. Wheat flour in the control diet was replaced with $5\%$ and $10\%$ Sargassum meal. Three replicate groups of fish average weighing 4.0 g were fed one of three isonitrogenous$(45\%)$ and isocaloric (14.5 MJ /kg diet) diets for 7 weeks. Survival of all groups were above $80\%$. Weight gain, feed efficiency and protein efficiency ratio were not significantly affected by dietary Sargassum meal levels (P>0.05). There were no significant differences (P>0.05) in moisture, crude protein, crude lipid, crude ash and fatty acid compositions of whole-body fish among groups. It is concluded that Sargassum meal could be used as a dietary additive or alternative low-cost dietary ingredient up to $10\%$ for juvenile ayu reared in seawater.

• Journal of fish pathology
• /
• v.22 no.3
• /
• pp.401-406
• /
• 2009

On May in 2008, mortality of anadromous Ayu Plecoglossus altivelis was observed on the Gangjeong river in Jeju. Major symptoms of the infected fish were mouth rot and skin ulcer. The causative agent was suspected as gliding bacteria. After culture on Shu-Shott and R2A media, we isolated bacterium belonging to the Flavobacterium from ayu with symptoms. As a result, the bacterium was identified as Flavobacterium succinicans JMFL55 by 16S rDNA sequence alignment with F. succinicans DSM 4002(98.27% similarity, GenBank accession NO. AM230492).

• Journal of Aquaculture
• /
• v.12 no.2
• /
• pp.145-153
• /
• 1999

This study was conducted to determine the protein requirement of ayu (Plecoglossus altivelis). Two replicate groups of fish initially averaging 6.6 g were fed the five isocaloric diets containing different protein level from 29% to 57% in a flow-through freshwater system for 25 days. White fish meal was used as a sole protein source. Weight gain and feed efficiency of fish increased significantly with dietary protein level up to 43% (P＜0.05) with no additional response above this level. Protein and lipid retention, moisture, protein and lipid contents of body were not affected by dietary protein levels (P>0.05). Daily protein intake increased significantly with dietary protein level, whereas protein efficiency ratio of fish fed the 57% dietary protein decreased (P<0.05). The data obtained in this study indicate that a 43% dietary protein level could be recommended for the optimum growth of ayu.

• Journal of Aquaculture
• /
• v.16 no.2
• /
• pp.69-75
• /
• 2003

Genetic variability and population structure of 11 natural ayu, Plecoglossus altivelis populations and one hatchery stock were assessed by starch gel electrophoretic analysis with 10 enzyme coding loci. Three loci were polymorphic (lower than 0.95 in major allele frequency) in natural populations,2 loci in hatchery stock. The average number of alleles per locus was 1.38. Observed heterozygosities ranged from 0.0235 to 0.088 (0.055 on the average) in natural population while 0.0925 in hatchery stock. The genetic distance among natural populations measured 0.000047-0.005407 and no significant differentiation was observed among them. On the other hand, a signifcant genetic distance was found between natural populations and the hatchery stock with measuring 0.002032-0.O08605. The results in this study suggest that the hatchery stock has diverged from natural populations, and also that careful to maintain sustainable and effective population size (parents number) should be made.