• Asian-Australasian Journal of Animal Sciences
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• v.29 no.9
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• pp.1353-1362
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• 2016

Hanwoo, a Korean native cattle (Bos taurus coreana), has great economic value due to high meat quality. Also, the breed has genetic variations that are associated with production traits such as health, disease resistance, reproduction, growth as well as carcass quality. In this study, next generation sequencing technologies and the availability of an appropriate reference genome were applied to discover a large amount of single nucleotide polymorphisms (SNPs) in ten Hanwoo bulls. Analysis of whole-genome resequencing generated a total of 26.5 Gb data, of which 594,716,859 and 592,990,750 reads covered 98.73% and 93.79% of the bovine reference genomes of UMD 3.1 and Btau 4.6.1, respectively. In total, 2,473,884 and 2,402,997 putative SNPs were discovered, of which 1,095,922 (44.3%) and 982,674 (40.9%) novel SNPs were discovered against UMD3.1 and Btau 4.6.1, respectively. Among the SNPs, the 46,301 (UMD 3.1) and 28,613 SNPs (Btau 4.6.1) that were identified as Hanwoo-specific SNPs were included in the functional genes that may be involved in the mechanisms of milk production, tenderness, juiciness, marbling of Hanwoo beef and yellow hair. Most of the Hanwoo-specific SNPs were identified in the promoter region, suggesting that the SNPs influence differential expression of the regulated genes relative to the relevant traits. In particular, the non-synonymous (ns) SNPs found in CORIN, which is a negative regulator of Agouti, might be a causal variant to determine yellow hair of Hanwoo. Our results will provide abundant genetic sources of variation to characterize Hanwoo genetics and for subsequent breeding.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.15
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• pp.5977-5982
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• 2014

Research over the years has progressively shown substantial broadening of the tumor necrosis factor alpha-related apoptosis-inducing ligand (TRAIL)-mediated signaling landscape. Increasingly it is being realized that pancreatic cancer is a multifaceted and genomically complex disease. Suppression of tumor suppressors, overexpression of oncogenes, epigenetic silencing, and loss of apoptosis are some of the extensively studied underlying mechanisms. Rapidly accumulating in vitro and in vivo evidence has started to shed light on the resistance mechanisms in pancreatic cancer cells. More interestingly a recent research has opened new horizons of miRNA regulation by DR5 in pancreatic cancer cells. It has been shown that DR5 interacts with the core microprocessor components Drosha and DGCR8, thus impairing processing of primary let-7. Xenografting DR5 silenced pancreatic cancer cells in SCID-mice indicated that there was notable suppression of tumor growth. There is a paradigm shift in our current understanding of TRAIL mediated signaling in pancreatic cancer cells that is now adding new layers of concepts into the existing scientific evidence. In this review we have attempted to provide an overview of recent advances in TRAIL mediated signaling in pancreatic cancer as evidenced by findings of in vitro and in vivo analyses. Furthermore, we discuss nanotechnological advances with emphasis on PEG-TRAIL and four-arm PEG cross-linked hyaluronic acid (HA) hydrogels to improve availability of TRAIL at target sites.

• Asian-Australasian Journal of Animal Sciences
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• v.1 no.2
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• pp.73-79
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• 1988

Experiments were conducted to determine whether supplemental cobalt (Co) or nickel (Ni) would prevent the signs of zinc (Zn) deficiency in chicks fed a high calcium (1.5%) corn-soybean diet and to examine the difference in $^{65}Zn$ absorption rates between inbred Ancona and crossbred New Hampshire X Single Comb White Leghorn chicks. In the initial experiment, the supplementation of 27 ppm Ni, 27 ppm Co or 54 ppm Co to a basal diet increased weight gain and reduced feather defects; 54 ppm Ni tended to increase weight gain but did not reduce feather defects. In further experiments, chicks fed the diet supplemented with 54 ppm Co usually showed increased weight gain and reduced feather defects; however, chicks fed a diet supplemented with 54 ppm Ni less frequently showed these effects. In another test, Ancona chicks fed a diet supplemented with 30 ppm Zn (except during a $^{65}Zn$ absorption study period) showed lower weight gain, more feather defects and less $^{65}Zn$ absorption than did New Hampshire X Leghorn cross chicks. Similar results were achieved with two strains of chicks fed the basal and 54 ppm Ni, 54 ppm Co or 60 ppm Zn supplemented diets. The sparing effects of Co on Zn which were commonly observed and the lesser effect of Ni or Zn were shown to be, at least in part, the result of increased availability of dietary Zn. That Ancona chicks required more Zn than New Hampshire cross chicks for the development of feathers and for growth is partly the result of decreased Zn absorption from the type of diets fed.

• Journal of Information Processing Systems
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• v.5 no.1
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• pp.33-40
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• 2009

Ever since the network-based malicious code commonly known as a 'worm' surfaced in the early part of the 1980's, its prevalence has grown more and more. The RCS (Random Constant Spreading) worm has become a dominant, malicious virus in recent computer networking circles. The worm retards the availability of an overall network by exhausting resources such as CPU capacity, network peripherals and transfer bandwidth, causing damage to an uninfected system as well as an infected system. The generation and spreading cycle of these worms progress rapidly. The existing studies to counter malicious code have studied the Microscopic Model for detecting worm generation based on some specific pattern or sign of attack, thus preventing its spread by countering the worm directly on detection. However, due to zero-day threat actualization, rapid spreading of the RCS worm and reduction of survival time, securing a security model to ensure the survivability of the network became an urgent problem that the existing solution-oriented security measures did not address. This paper analyzes the recently studied efficient dynamic network. Essentially, this paper suggests a model that dynamically controls the RCS worm using the characteristics of Power-Law and depth distribution of the delivery node, which is commonly seen in preferential growth networks. Moreover, we suggest a model that dynamically controls the spread of the worm using information about the depth distribution of delivery. We also verified via simulation that the load for each node was minimized at an optimal depth to effectively restrain the spread of the worm.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.19
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• pp.59-68
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• 1975

A soybean variety KWANG KYO was planted on the hilly land and fertile soil, and concentrated superphosphate and fused phosphate were applied on calcium hydroxide treated soil at level of 2.5%, 5% and 7.5% of phosphate absorbtion coefficient of soil. Application of phosphate fertilizer and lime caused higher grain yield in hilly land soil than fertile soil. The effect of fused phosphate on soybean yield was superior to concentrated superphosphate, and the effect of lime on fused phosphate availability was not significant. When the two phosphate sources were compared at equal $P_2O_5$ basis, fused phosphate required higher amount than concentrated super-phosphate. Negative correlation was obtained between potassium content and Ca + Mg in soybean leaf and in soil respectively.

• Economic and Environmental Geology
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• v.41 no.1
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• pp.151-163
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• 2008

Since the country's opening to the world, China's rapid economic growth has resulted in strong demand for a wide variety of mineral commodities. During the same period, China's mineral production increased sharply. China's increased mineral production and demand affected the worldwide availability of minerals and metals, the market prices, and trade. Ministry of Land and Resources of China (MLR) planned to build as many as 10 strategic mineral reserve, including reserves of aluminium, copper, manganese, uranium, and other mineral that the country urgently needed. In white paper of China's mineral resources, China is planning the mineral resources conservation and its reasonable utilization, domestic supply increase of resources, activation of cooperative development with foreign partners, balance in resources development and environment conservation, and sustainable resources management as the mineral resources policies for the China's economic development.

• Korean Journal of Organic Agriculture
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• v.20 no.4
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• pp.491-502
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• 2012

The world's food production systems are becoming an area of great concern for both human and ecological health. Research has identified that one of the greatest threats to sustainability is conventional industrial agricultural systems and the high energy and material cost they require to function. The organic food movement is contributing as promising alternative to the current dominant model. Over the last 50 years it has developed into the most visible brand for a healthier and more environmentally sustainable food system. However, to achieve its full potential there are still a number of hurdles which must be overcome to make organic products a more viable and appealing option for consumers. This paper provides an overview of key research that has been conducted on why consumers are buying organic products, what they are buying and what is preventing them from purchasing more. It concludes that the key challenge is for the organic food movement to convince existing consumers of the superior 'value' of its products. In addition there are a number of methodological issues associated with analysing the market for organic products as well as issues of limited distribution, intermittent availability and high prices that are currently preventing increases in sales. Recognition and management of these barriers could contribute to more effective targeted research into consumer food purchasing motivations and subsequently the development of more sophisticated marketing strategies that assist in maintaining integrity with consumers and fending off challenges from conventional as well as other complementary food systems such as local food movement. And finally to achieve these market growth strategies the organic food movement will need to cope with its diverse constituency - ranging from global corporates through to local production and consumption - and provide attractive opportunities to individuals and business at all stages in supply chain whilst retaining credibility with government to ensure ongoing policy support.

• Journal of the Korea Society of Computer and Information
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• v.12 no.4
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• pp.33-42
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• 2007

Accurate predictions of software release times, and estimation of the reliability and availability of a software product require quantification of a critical element of the software testing process : test coverage. This model called Enhanced non-homogeneous poission process(ENHPP). In this paper, exponential coverage and S-shaped model was reviewed, proposes the Kappa coverage model, which maked out efficiency application for software reliability. Algorithm to estimate the parameters used to maximum likelihood estimator and bisection method, model selection based on SSE statistics and Kolmogorov distance, for the sake of efficient model, was employed. From the analysis of mission time, the result of this comparative study shows the excellent performance of Burr coverage model rather than exponential coverage and S-shaped model using NTDS data. This analysis of failure data compared with the Kappa coverage model and the existing model(using arithmetic and Laplace trend tests, bias tests) is presented.

• Journal of the Korean Society for Precision Engineering
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• v.23 no.5 s.182
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• pp.143-148
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• 2006

It is still very difficult to generate a geometry model and finite element model, which has complex and many free surface, even though 3D CAD solutions are applied. Furthermore, in the medical field, which is a big growth area of recent years, there is no drawing. For these reasons, making a geometry model, which is used in finite element analysis, is very difficult. To resolve these problems and satisfy the requests of the need to create a 3D digital file for an object where none had existed before, new technologies are appeared recently. Among the recent technologies, there is a growing interest in the availability of fast, affordable optical range laser scanning. The development of 3D laser scan technology to obtain 3D point cloud data, made it possible to generate 3D model of complex object. To generate CAD and finite element model using point cloud data from 3D scanning, surface reconstruction applications have widely used. In the early stage, these applications have many difficulties, such as data handling, model creation time and so on. Recently developed point-based surface generation applications partly resolve these difficulties. However there are still many problems. In case of large and complex object scanning, generation of CAD and finite element model has a significant amount of working time and effort. Hence, we concerned developing a good direct finite element model generation method using point cloud's location coordinate value to save working time and obtain accurate finite element model.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.277-283
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• 2009

One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.