• The Journal of Advanced Prosthodontics
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• v.8 no.4
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• pp.313-320
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• 2016

PURPOSE. The aim of this study was to evaluate the clinical findings and patient satisfaction on implant overdenture designed with Locator implant attachment or Locator bar attachment in mandibular edentulous patients. MATERIALS AND METHODS. Implant survival rate, marginal bone loss, probing depth, peri-implant inflammation, bleeding, plaque, calculus, complications, and satisfaction were evaluated on sixteen patients who were treated with mandibular overdenture and have used it for at least 1 year (Locator implant attachment: n=8, Locator bar attachment: n=8). RESULTS. Marginal bone loss, probing depth, plaque index of the Locator bar attachment group were significantly lower than the Locator implant attachment group (P<.05). There was no significant difference on bleeding, peri-implant inflammation, and patient satisfaction between the two denture types (P>.05). The replacement of the attachment components was the most common complication in both groups. Although there was no correlation between marginal bone loss and plaque index, a significant correlation was found between marginal bone loss and probing depth. CONCLUSION. The Locator bar attachment group indicates lesser marginal bone loss and need for maintenance, as compared with the Locator implant attachment group. This may be due to the splinting effect among implants rather than the types of Locator attachment.

• Clinical and Experimental Reproductive Medicine
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• v.49 no.4
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• pp.248-258
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• 2022

Objective: This research investigated the effects of human chorionic gonadotropin (HCG)-producing peripheral blood mononuclear cells (PBMCs) on the implantation rate and embryo attachment in mice. Methods: In this experimental study, a DNA fragment of the HCG gene was cloned into an expression vector, which was transfected into PBMCs. The concentration of the produced HCG was measured using enzyme-linked immunosorbent assay. Embryo attachment was investigated on the co-cultured endometrial cells and PBMCs in vitro. As an in vivo experiment, intrauterine administration of PBMCs was done in plaque-positive female mice. Studied mice were distributed into five groups: control, embryo implantation dysfunction (EID), EID with produced HCG, EID with PBMCs, and EID with HCG-producing PBMCs. Uterine horns were excised to characterize the number of implantation sites and pregnancy rate on day 7.5 post-coitum. During an implantation window, the mRNA expression of genes was evaluated using real-time polymerase chain reaction. Results: DNA fragments were cloned between the BamHI and EcoRI sites in the vector. About 465 pg/mL of HCG was produced in the transfected PBMCs. The attachment rate, pregnancy rate, and the number of implantation sites were substantially higher in the HCG-producing PBMCs group than in the other groups. Significantly elevated expression of the target genes was observed in the EID with HCG-producing PBMCs group. Conclusion: Alterations in gene expression following the intrauterine injection of HCG-producing PBMCs, could be considered a possible cause of increased embryo attachment rate, pregnancy rate, and the number of implantation sites.

• The Korean Journal of Food And Nutrition
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• v.13 no.2
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• pp.139-145
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• 2000

To separate anticaries and antiinflammation from Aloe vera peel, we investigated a inhibited effect of Streptococcus mutans JC-2 that was antibiosis, glucosyltransferase activity about aloe-emodin and barbaloin. Aloe-emodin and barbaloin had strong antibiosis activity against Streptococcus mutans JC-2, they were especially antibiosis effect to low growth and prolong lag phase at attachment concentration 100$\mu\textrm{g}$/mL. The reduction rate of a culture fluid became to lessen than the comparison group for aloe-emodin and barbaloin. The intracellular materials of Streptococcus mutans JC-2 were to leakage as much as attachment concentration addition of aloe-emodin and barbaloin but there was no significant difference membrane demage between two active substances. The activity of GTase was inhibited by aloe-emodin and barbaloin and their inhibition rate was respectively 99.8%, 98.4% at the attachment concentration 100$\mu\textrm{g}$/mL.

• Child Health Nursing Research
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• v.25 no.2
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• pp.95-102
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• 2019

Purpose: The purpose of this study was to identify factors associated with career maturity among Korean high school students. Methods: A descriptive cross-sectional design was adopted using secondary data from the 2012 Korean Welfare Panel Study (KoWePS). The participants were 496 high school students who completed the supplemental survey for children, which included items on career maturity, self-esteem, study stress, teacher attachment, relationship with parents, peer attachment, depression and anxiety. Descriptive statistics, the chi-square-test, the t-test, and a decision tree were used for data analysis. Results: The decision tree identified five final nodes predicting career maturity after forcing self-esteem as the first variable. The highest predicted rate of high career maturity was associated with high self-esteem, experience of career counseling, and high teacher attachment. The lowest predicted rate of high career maturity was associated with low self-esteem and low attachment to friends. Conclusion: Factors influencing career maturity were varied by levels of self-esteem in Korean high school students. Thus, it is necessary to develop different approaches to enhance career maturity according to levels of self-esteem.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.5
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• pp.660-666
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• 2023

This study aimed to examine the effect of external pop-up satellite archival tags (PSATs) attachment and water temperature on the oxygen consumption rate (OCR) of the olive flounder (mean body weight 2281.7 g). The OCRs of fish were measured under conditions of three different water temperature conditions (15, 20, and 25℃) and two different tagging methods [non-tagging, control; bio-logger external attachment with a miniature PSAT (dummy mrPAT), BEA] using a closed flow-through respirometer. The OCRs of fish linearly increased with the increase in water temperature in both the control and BEA (P<0.001); however, the OCRs of BEA were approximately 1.8-1.9 times lower than those of the control at each water temperature (P<0.001). The Q₁₀ values of the control and BEA were the highest in the water temperature range of 15 to 20℃, but sensitivity to water temperature changes was higher in BEA than in the control. The metabolic energy loss rate (MEL) of fish increased with increasing water temperature regardless of external tagging, but the MEL of the control was higher than that of BEA (P<0.001). These results demonstrate that OCR, thermal sensitivity, and energy expenditure are all affected in adult olive flounder with external PSAT attachment.

• Environmental Analysis Health and Toxicology
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• v.25 no.2
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• pp.161-168
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• 2010

The observed the toxic effect of WSF (Water Soluble Fraction) on development time, development rate, attachment rate, survival rate and growth of the larva during the early stage of the Pacific oyster, Crassostrea gigas. The time required early juvenile from the fertilized eggs exposed to crude oil (WSF) was about 13 hours in control group. But that of the crude oil dose group takes 12 hours, with shorter compared to control group. Development and attachment rate of the oyster significantly higher in 0.4 and 0.8 mg/L WSF compared to control group (P<0.05) but significantly lower more than 1.6 mg/L WSF (P<0.05). Survival rate of the oyster larva significantly higher in 0.4 and 0.8 mg/L WSF compared to control group (P<0.05) but significantly lower more than 2.4 mg/L WSF (P<0.05). Survival rate of spot showed no significantly difference compared to control group (P>0.05). Shell growth of the oyster were significantly lower more than 1.6 mg/L WSF compared control group (P<0.05). The effect concentration of crude oil showed that more 0.4 mg/L in development and attachment rate and more 1.6 mg/L in growth. Also, chronic exposure of crude oil even effect also in low concentration.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.2
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• pp.91-95
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• 2009

To understand the effect of attachment substrate on the growth of benthic microalgae, we experimentally investigated the growth of benthic microalgae Nitzschia sp. (Jinhae Bay strain) with additions of glass beads in different sizes. The glass beads used in this study are 0.09-0.15 mm (G1), 0.25-0.50 mm (G2), 0.75-1.00 mm (G3) and 1.25-1.65 mm (G4). No addition of glass beads used as controls. Highest specific growth rate (0.37/day) and maximum cell density ($9,232{\pm}840$ cells/mL) of Nitzschia sp. showed at the smallest glass beads (G1), and the specific growth rate and maximum cell density were decreasing with increasing size of glass beads (specific growth rate and maximum cell density of G4 was 0.24/day and $6,397{\pm}524$ cells/mL, respectively). Moreover, specific growth rate of the control experiment (0.23/day) was significantly lower than their of G1 to G3 experiment. The results indicated that the attachment substrate for benthic microalgae as Nitzschia sp. is important factor which affecting the growth rate as well as cell density. Therefore, the physiological experiment of benthic microalgae seems to be necessary to preliminary experiment, which is addition or not of the attachment suitable substrate and the grain size for the target species of benthic microalgae.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.222-230
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• 1995

This study was canied out to ddermine optimum areas for various sizes of land coverage of the farm machinery utilization in 1993-1994. A kind of machinery size and work systems were classed as the power tiller of 10HP+man power, the tractor of 35~46HP (tractor of 64~86HP and attachment were leased to harvest work), 64-86HP+ attachment and 90- 105HP+ attachment, respectively. \ulcornerhe results are summarized as follows: 1. The optimum areas of tractors of 90~105HP, 64~86HP and the power tiller of lOHP were estimated as 21.9 (corn-rye cropping system)- 26.9ha (sorghum $\times$ sudangrass - rye cropping system), 14.7 - 22.8ha and 1.2 - 1.61ha, respectively. The break-even-point areas of the tractors of 90-105HP. 64-86HP and the power tiller of lOHP were 16.6 (corn-rye cropping system)- 19.9ha (sorghum $\times$ sudangrass - rye cropping system), 12.5 - 16.lha and 0.12-0.13ha, respectively. 2. The optimum areas (land sizes, annual field capacity) for 50 cows by feeding rate(%) of roughage to concentrate were 6.8ha, 13.6ha in the 4060, 8.5ha, 17.0ha in the 5050 and 10.2ha, 20.4ha in the 60:40, and in case of 30 cows, it were 4.lha, 8.2ha in the 40:60, respectively. In the former case for the form of work system was the trador of 90-105HP+attachment and 64~86HP+ attachment, and the latter was the tractor of 35~46HP (tractor of 64~86HP and attachment were leased to harvest work) and 64-86HP+ attachment. 3. Productiori cost for corn-rye cropping system reducted to 51.8% in 102.9 wonkg dry matter the tractor of 90~ 105HP+ attachment with 213.4 wonkg dry matter the power tiller of 10HP+ man power.

• Journal of Korean Society on Water Environment
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• v.37 no.1
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• pp.10-19
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• 2021

In the flotation process to remove microplastic (MP) particles, the attachment and separation efficiency is determined by the basic physicochemical characteristics of MP particles as well as bubbles. To evaluate the flotation characteristics of MP particles, we carried out a series of simulations using the population balance (PB) model. The initial attachment coefficient (αo) of MP particles was in the range of 0.2-0.275, and it was slightly lower than that of typical particles, such as clay, debris and algae particles, which exist in water bodies, αo, 0.3-0.4. The relative bubble number (RBN) attached to the surface of the typical number of bubbles was 0.30 and 0.32 for MP 30 ㎛ and MP 58 ㎛, respectively. In comparison, the RBN of larger MP particles (138 ㎛) was as high as 0.53. Furthermore, smaller microbubbles were required to separate properly or additional treatment needed to be applied to enhance collision and attachment efficiency since the flotation of MP particles was found to be difficult to treat as high-rate. As a result of comparing the removal rate (experimental value) of MP particles obtained from the batch-type flotation apparatus and the flotation removal rate (predicted value) of MP obtained through the PB model, the final particles by the particle size of MP overall except for the initial separation time area. With respect to the removal efficiency, the observed and predicted values were similar, and it was confirmed that the floating separation characteristics and evaluation of the MP particles through the PB model could be possible.

• Journal of Periodontal and Implant Science
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• v.31 no.3
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• pp.513-529
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• 2001

For reconstruction of the bony defect, various artificial substitutes were developed. Among them, there has been a study of calcium phosphate coated bone substitutes for increasing attachment of osteoblasts in vivo. The purpose of this study was to evaluate the effects of serum and platelet-rich plasma (PRP) on calcium phosphate coated culture plate for the initial attachment, proliferation and activity of osteoblasts. After sampling the blood from white rats and concentrating by centrifugation, the amount of attachment of PDGF-BB and $TGF-{\beta}$ on the calcium phosphate coated culture plate was measured. Cultured HOS and ROS 17/2.8 cell was measured on attachment level and proliferation rate of osteoblasts. Alkaline phosphatase activity of HOS and ROS 17/2.8 cell was measured for studying on the activating rate of osteoblast. 1. Counting the amount of platelets of seperated plasma and PRP, the average number of platelets was 177,003 $cell/{\mu}l$ in plasma, and 1,656,062 $cell/{\mu}l$ in PRP, which was about 9 times as high as in plasma. 2. Amount of PDGF-BB deposited at calcium phosphate coated plate had increased by the total amount of plasma and PRP on the culture plate, whereas $TGF-{\beta}$had been deposited on the plate only when treated by $50{\mu}{\ell}$ of PRP(p<0.01). 3. After plating serum and PRP for 3 hours, we attached with HOS and ROS17/2.8 cell for 1 hour and 4 hours. There were no significant difference of the attachment between serum and control group, whereas there were significantly difference of the attachment between depositioning of PRP and control group. 4. After attaching plasma and PRP for 3 hours, cell number has much increased when HOS and ROS17/2.8 cell had been cultured for 48 hours(p<0.05). 5. After attaching plasma and PRP for 3 hours, concentration of alkaline-phosphatase has increased when HOS and ROS17/2.8 cell had been cultured for 48 hours(p<0.01). These results suggested that PRP affected on initial cell attachment rather than proliferation and activation of osteoblasts at calcium phosphate coated plate.