• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.617-623
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• 2008

A fine-mapping method exploiting linkage disequilibrium was used to detect quantitative trait loci (QTL) on the X chromosome affecting milk production, body conformation and productivity traits. The pedigree comprised 22 paternal half-sib families of Black-and-White Holstein bulls in the Netherlands in a grand-daughter design for a total of 955 sons. Twenty-five microsatellite markers were genotyped to construct a linkage map on the chromosome X spanning 170 Haldane cM with an average inter-marker distance of 7.1 cM. A covariance matrix including elements about identical-by-descent probabilities between haplotypes regarding QTL allele effects was incorporated into the animal model, and a restricted maximum-likelihood method was applied for the presence of QTL using the LDVCM program. Significance thresholds were obtained by permuting haplotypes to phenotypes and by using a false discovery rate procedure. Seven QTL responsible for conformation types (teat length, rump width, rear leg set, angularity and fore udder attachment), behavior (temperament) and a mixture of production and health (durable prestation) were detected at the suggestive level. Some QTL affecting teat length, rump width, durable prestation and rear leg set had small numbers of haplotype clusters, which may indicate good classification of alleles for causal genes or markers that are tightly associated with the causal mutation. However, higher maker density is required to better refine the QTL position and to better characterize functionally distinct haplotypes which will provide information to find causal genes for the traits.

• Journal of the Korean Academy of Clinical Electrophysiology
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• v.7 no.1
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• pp.7-10
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• 2009

Purpose : The purpose of this study was to investigate the effect of electrical stimulation applied in dominant forearm on autonomic nervous system response of both hands. Methods : Fourteen healthy subjects (women) received low frequency-high intensity electrical stimulation to one forearm. The subjects assigned to two groups; a ipsilateral stimulation group (n=7) and a contralateral stimulation group (n=7). The electrode attachment was arranged on the forearm of the dominant arm and the electricity stimulus time was set as 15 minutes. Measuring items were the skin conduction velocity, the blood flow, and the pulse rate, which were measured total 3 times (pre, post, and post 10 min.). Results : The skin conduction velocity showed a significant difference according to the change of the time in both hands, but there was no significant difference according to time in the blood flow, and the change of the pulse frequency regardless of stimulus side. Conclusion : These results demonstrate that the low frequency-high intensity electrical stimulation applied dominant forearm can increase selectively only with the skin conduction velocity, which may be helpful for the activation of the sudomotor function of both hands by the activation of sympathetic nerve.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.141-144
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• 2000

Highly open porous polymer matrices are required for high density cell seeding, efficient nutrient, and oxygen supply to the cells cultured in the three dimensional matrices. However, there are severe problems of mass transfer limitations within the cell/scaffolds culture system. Thus we hypothesize that continuos-flow culture conditioning of cells with the scaffolds may improve the cell viability and the differentiated function. In this study, we fabricated porous PLGA scaffolds by using gas-foaming/salt-leaching method as previous described. Viscous PLGA gel paste contains ammonium bicarbonate particulates, acting as a gas-foaming agent as well as a salt-leaching porogen, were cast into Teflon mold and dried. Ammonium bicarbonate salt upon contact to an acidic aqueous solution evloves gaseous ammonia and carbon dioxide by itself. And we conjugated galactose moiety [AGA; $N-(aminobuty1)-O-{\beta}-D-galactopyranosyl-(1{\rightarrow}4)-D-glucoamide]$ to the terminal end group of a PLGA to increase the cell adhesion and matain the differentiated function of hepatocytes. Cell-seeded scaffolds were secured in a flow bioreactor chamber and exposed to continuous flow at 5 ml/min. As a result of our study, the high yield of hepatocytes attachment was accomplished by increasing the concentration of PLGA-AGA conjugate in polymer scaffolds and cells in the scaffolds under continuos flow condition maintained a high level of viability and albumin secretion rate of cultured hepatocytes showed a higher level that of control groups.

• Journal of Periodontal and Implant Science
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• v.30 no.3
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• pp.651-661
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• 2000

A mucogingival grafting procedure has been developed to cover denuded root surface. The subepithelial connective tissue graft technique is very predictable and allows for a good esthetic results and minimum patient discomfort on the palate. However, in areas where there is a lack of vestibular depth and keratinized attached tissue, the presence of frena or heavy muscle attachment, covering the connective tissue graft with a mucosal flap is very difficult. The purpose of this study is to evaluate an alternative technique of root coverage using the free connective tissue graft. The results were as follows: 1. Probing depths didn't seem to vary significantly from the preoperative to postoperative period. 2. The amount of keratinized tissue showed an increase of $5.9{\pm}0.97mm$ from the preoperative level. 3. Total clinical exposed root coverage increase 72.2% compare with preoperative level. 4. The shrinkage from gingival margin is $4.2{\pm}1.15mm$ and the mean shrinkage rate is 40.1%. 5. The depth of the vestibule increased with the average distance from cementoenamel junction to mucogingival junction being $7.4{\pm}1.65mm$.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.15 no.1
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• pp.8-16
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• 2006

The communication through optical fiber is taking an important role of the expansion of communication network with excellent transmitting rate and quality. As the optical communication is introduced to the backbone network at first and becomes a general communication method of network, the demand of kernel parts of optical communication such as PLC(Planar Light Circuit), Coupler, and WDM(Wavelength Division Multiplexing) element increases. The alignment and the attachment technology are very important in the fabrication of optical elements. In this paper, the driving mechanism of ultra precision stage is studied with the aim of optimal design of stage. The travel and the resolution of stage are investigated. The hysteresis of the stage is generated because of PZT actuator. The hysteresis and the inverse hysteresis are modeled in X, Y, and Z-axis motion. The input data of desired displacement of the stage according to input voltage is obtained from the inverse hysteresis equation. In the result of experiments with the input data, the errors due to hysteresis are well compensated.

• Journal of Biosystems Engineering
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• v.27 no.3
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• pp.235-240
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• 2002

Performance of the heat pump with attaching an air heat exchanger was investigated in the heating condition when the air heat exchanger was worked in the ambient air temperature of -5 to 11$\^{C}$ and air flow rate of 542 to 747 ㎡/h. Performance tests for heating condition were conducted in an experimental room equipped with heat pump. The performance tests were performed in a ambient temperature of -4 ∼ 11$\^{C}$, and room temperature of 4∼22$\^{C}$ respectively. Measured data(temperature, capacity of heat transfer and consumption of electronic power) were analyzed to the efficiency of HEEVA(Heat Exchanger fur the Evaporator), overall heat transfer coefficient and COP of heat pump. The results of inlet temperature for evaporator increased that the temperature was 2 ∼6$\^{C}$, and inlet temperature for condenser decreased that the temperature was 3 ∼ 8$\^{C}$. The results of comparing efficiency of HEEVA for the ratio of heat exchange between hot air and cold air showed that efficiency were considered to 91% because of the ratio of 83∼98%. The results of comparing of COP for the heat pump increased that improvement COP was approximately 0.3∼7.5 than HEEVA had not been operated.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2001.05b
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• pp.129-132
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• 2001

The purpose of this research was to understand possibility of foodwaste treatment by hybrid anaerobic reactor(HAR). The Possibility of methane utility and applicability of hybrid reactor system using foodwaste as substrate was investigated. The maximum loading rate and optimized operational conditions were determined. Hybrid anaerobic reactor was filled with packing material 50% of its total volume between the tube and the outer surface. The packing material used was randomly packed open-pore synthesis activated ceramic(SAC) media as support media for microbial attachment, growth, and chemical stability protected bacteria from effect of organic acid accumulation. In this research, although foodwaste has high concentrations C $l^{[-10]}$ and S $O_{4}$$^{2-}$ concentration the possibility of foodwaste anaerobic treatment was when foodwaste is treated by anaerobic digestion, this study focused on the possibility using C $H_4$ gas made under the anaerobic treatment as an alternative energy source. Other objective of this research is to study struvite formation and crystal forms in anaerobic digester. HAR is used to investigate phosphate crystallization without the addition of chemicals.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.48 no.6
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• pp.469-474
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• 1999

Methods and systems to remove static electricity are requested in the field of industry because the static electricity causes a flammable gas explosion or fire and a reduction of production rate in manufacturing semiconductor devices and so on. This paper is a basic study about a new structure of electrode system to control the quantities of generated ions and to solve the problem of dust attachment to needle electrode. In addition, a new type field controlled electrostatic charge neutralizer was proposed, and it could control the electric field in the end of the needle electrode by controlling the voltage of the third electrode around the tip of the needle electrode. As aresult, it was possible to control the quantities of generated ion by controlling the electric field in the needle electrode with the third electrode, which shows the possibilities to solve the nonequilibrium of generated ions in ac power source and the problem of the dust in the needle electrode.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.134.1-134.1
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• 2017

In this study, we propose a new scaffold fabrication method, "direct electro-hydrodynamic jet process," using the initial jet of an electrospinning process and ethanol media as a target. The fabricated threedimensional (3D) fibrous structure was configured with multilayered microsized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes. The fabrication of the structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous scaffold was cultured with preosteoblasts to observe the initial cellular activities in comparison with a solid-freeform fabricated 3D scaffold sharing a similar structural geometry. The cell-culture results showed that the newly developed scaffold provided outstanding microcellular environmental conditions to the seeded cells (about 3.5-fold better initial cell attachment and 2.1-fold better cell proliferation).

• Journal of fish pathology
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• v.21 no.3
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• pp.167-174
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• 2008

Few studies on parasitic scutica-like ciliates (SLCs) influencing larval growth and survival during the seed production of the Japanese scallop, Patinopecten yessoensis have been performed in spite of relatively high magnitude of their infectious damage to the larvae. In the mass seed production of the scallop, SLCs were never infectious during D-larval stage which lasted 6 to 7 days. Indeed, the infection first occurred in 2 to 4 days after first umbo-staged larvae. A regular selection of active larvae in swimming behavior kept larval SLC infection low throughout the culture compared to unselected control (P<0.05) in which all the larvae alive were contained. Higher infection frequencies of the unselected control resulted in lower larval survivals and wider range of larval size distribution that drove larval attachment rate lower. To the worse, the nursery spats from the higher SLC infection exhibited a retarded growth and elevated occurrence of abnormal scallop.