• Biotechnology and Bioprocess Engineering:BBE
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• 제9권4호
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• pp.285-291
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• 2004

A simulated moving bed (SMB) chromatography system is a powerful tool for preparative scale separation, which can be applied to the separation of chiral compound. We have de-signed our own lab-scale SMB chromatography using 5 HPLC pumps, 6 stainless steel columns and 4 multi-position valves, to separate a racemic mixture of ketoprofen in to its enantiomers. Our design has the characteristics of the low cost for assembly for the SMB chromatography and easy repair of the unit, which differs from the designs suggested by other investigators. It is possible for the flow path through each column to be independently changed by computer control, using 4 multi-position rotary valves and 5 HPLC solvent delivery pumps. In order to prove the operability of our SMB system, attempts were made to separate the (S)-ketoprofen enantiomer from a ketoprofen racemic mixture. The operating parameters of the SMB chromatography were calculated for ketoprofen separation from a batch chromatography experiment as well as by the triangle theory. With a feed concentration of 1 mg/mL, (S)-ketoprofen was obtained with a purity of 96% under the calculated operating conditions.

• 한국공작기계학회논문집
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• 제14권2호
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• pp.27-32
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• 2005

This paper presents the optimization of expanding velocity for tube expanding process in the manufacturing of a heat exchanger. In specific, the expanding velocity has a great influence on the performance of a heat exchanger because it is a key variable determining the quantity of tube expending at assembly stage as well as a key Parameter determining overall production rate. The simulation showed that the genetic algorithm used in this paper resulted in the optimal tube expanding velocity by performing the following series of iteration; the generation of arbitrary population for tube expanding parameters, consequently the generation of tube expanding velocities, the evaluation of tube expanding quantity using the pre-trained data of plastic deformation by means of a neural network and finally the generation of next population using a penalty faction and a Roulette wheel method.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2002년도 춘계학술대회 논문집
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• pp.567-572
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• 2002

The equipment of industrial robot in manufacturing and assembly lines has rapidly increased. In order to achieve high productivity and flexibility, it becomes very important to develop the visual feedback control system with Off-Line Programming System(OLPS ). We can save much efforts and time in adjusting robots to newly defined workcells by using Off-Line Programming System. A proposed visual calibration scheme is based on position-based visual feedback. The visual calibration system is composed of a personal computer, an image processing board, a video monitor, and one camera. The calibration program firstly generates predicted images of objects in an assumed end-effector position. The process to generate predicted images consists of projection to screen-coordinates, visible range test, and construction of simple silhouette figures. Then, camera images acquired are compared with predicted ones for updating position and orientation data. Computation of error is very simple because the scheme is based on perspective projection, which can be also expanded to experimental results. Computation time can be extremely reduced because the proposed method does not require the precise calculation of tree-dimensional object data and image Jacobian.

• 대한기계학회논문집A
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• 제24권6호
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• pp.1616-1623
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• 2000

A diaphragm spring is an important component of a clutch assembly, characteristics of which depends largely on that of a diaphragm spring. A diaphragm spring is subject to high stress concentration in driving condition, which frequently causes cracks and fracture around finger area. In this paper, behavior of a diaphragm spring is analysed by finite element method to calculate sensitivity of design parameters, which is used to perform optimal design of diaphragm spring shape. As an object function, hoop stresses are taken and minimized to improve durability. Characteristics of the diaphragm is used as equality constraint to maintain the original design purpose and sequential linear programming(SLP) is utilized as an optimization tool. With optimized design, it is verified that concentrated stress is decreased maintaining release load characteristic.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2001년도 추계학술대회(한국공작기계학회)
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• pp.140-145
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• 2001

The equipment of industrial robot in manufacturing and assembly lines has rapidly increased. In order to achieve high productivity and flexibility, it becomes very important to develop the visual feedback control system with Off-Line Programming System(OLPS). We can save much efforts and time in adjusting robots to newly defined workcells by using Off-Line Programming System. A proposed visual calibration scheme is based on position-based visual feedback. The visual calibration system is composed of a personal computer, an image processing board, a video monitor, and one camera. The calibration program firstly generates predicted images of objects in an assumed end-effector position. The process to generate predicted images consists of projection to screen-coordinates, visible range test, and construction of simple silhouette figures. Then, camera images acquired are compared with predicted ones for updating position and orientation data. Computation of error is very simple because the scheme is based on perspective projection, which can be also expanded to experimental results. Computation time can be extremely reduced because the proposed method does not require the precise calculation of tree-dimensional object data and image Jacobian.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1995년도 추계학술대회 논문집
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• pp.214-218
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• 1995

Much labor, an exceedingly long lead time, and the skills of experienced engineers are required for press tool design. To reduce such problems, several CAD systems for blanking or piercing have been developed. This paper describes a computer-aided design for blanking or piercing of irregularly shaped sheet metal products. An approach to the system is based on knowledge base rules. The process planning & die design system is designed by considering several factors, such as complexity of blank geometry, punch profile, and availability of press equipment and standard parts. Therefore, after checking a production feasibility for irregular shaped sheet metal products, this system which is implemented strip layout module can carry out a process planning and generate the strip layout in graphic forms. Also this system implemented die layout module can carry out a die design for each process which is obtained form the result of an automated process planning and generate parts and assembly drawing of a die set.

• 소음진동
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• 제9권4호
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• pp.841-848
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• 1999

직류 모터의 생산 공정품질 확인 작업에 있어서 전체 소음레벨이 합격점에 이른다 할지라도 관능 평가시의 이음으로 인해 불량판정을 받게되는 경우가 있다. 이런 종류의 문제는 가공 또는 조립 공정상에서 발생될수 있는데 본 눈문에서는 과도기적 스펙트럴 분석 혹은 시간-주파수 분석 기법을 소음품질 문제에 적용해 보았다.Case Study로써 윈드쉴드 와이퍼 모터의 이음 발생원이 상세 분석되었으며, 결과적으로 정상적인 주파수 분석에서 발견되지 않았던 아마추어 축의 불량이 충격소음을 일으킨것으로 밝혀졌다. 이를 통해 회전체-기어 시스템의 이음 원인규명을 위한 시간-주파수 분석 기법의 효과적 활용이 잘 나타났다.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.767-771
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• 2005

Expression in yeast may prove more amenable to generating large amounts of viral antigens for a vaccine candidate. We, therefore, cloned the gene encoding the Hepatitis C virus (HCV) structural proteins (C-El-E2, c740) fused in-frame with, and immediately 3' to, the chicken-lysozyme signal peptide (C-SIG) gene and under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter. In yeast, the HCV structural proteins were expressed in two different forms: a processed and a nonprocessed aggregated form. Biophysical characterization by sucrose linear gradient centrifugation revealed that both forms were present in the same fractions with a buoyant density of 1.127-1.176 g/$cm^3$. These findings suggest that the efficient synthesis of HCV structural proteins in yeast may be an important tool to study virus assembly and may lead to the development of an HCV vaccine.

• 해양환경안전학회지
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• 제21권2호
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• pp.194-199
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• 2015

본 연구에서는 도그 피스 설치량에 따른 용접 변형 실험을 수행하여 용접 변형 감소 효과를 정량적으로 평가하였고, 도그 피스 설치를 고려한 용접 변형 해석 방법을 제안하였다. 용접 변형 실험 결과, 도그 피스 설치량이 증가할수록 용접 변형이 감소하며 최대 74%의 변형 감소 효과가 있음을 확인하였다. 원판형 열속 모델을 사용한 열탄소성 유한요소 해석을 활용하여 도그 피스 설치를 고려한 용접 변형 해석 방법을 제안하고, 해석 결과와 실험 결과가 좋은 유사성을 보이고 있음을 확인하였다. 본 해석 방법은 현행 도그 피스 설치량에 대한 적정 여부를 평가하거나 도그 피스 사용량을 절감할 수 있는 설치 가이드를 마련하기 위한 해석적 툴로 활용될 수 있다.

• 한국미생물·생명공학회지
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• 제46권3호
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• pp.300-312
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• 2018

Whole-genome sequencing of the wood-rotting fungus, Flammulina fennae, was carried out to identify carbohydrate-active enzymes (CAZymes). De novo genome assembly (31 kmer) of short reads by next-generation sequencing revealed a total genome length of 32,423,623 base pairs (39% GC). A total of 11,591 gene models in the assembled genome sequence of F. fennae were predicted by ab initio gene prediction using the AUGUSTUS tool. In a genome-wide comparison, 6,715 orthologous groups shared at least one gene with F. fennae and 10,667 (92%) of 11,591 genes for F. fennae proteins had orthologs among the Dikarya. Additionally, F. fennae contained 23 species-specific genes, of which 16 were paralogous. CAZyme identification and annotation revealed 513 CAZymes, including 82 auxiliary activities, 220 glycoside hydrolases, 85 glycosyltransferases, 20 polysaccharide lyases, 57 carbohydrate esterases, and 45 carbohydrate binding-modules in the F. fennae genome. The genome information of F. fennae increases the understanding of this basidiomycete fungus. CAZyme gene information will be useful for detailed studies of lignocellulosic biomass degradation for biotechnological and industrial applications.