• Asian-Australasian Journal of Animal Sciences
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• v.21 no.3
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• pp.426-433
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• 2008

Heat stress is major welfare problem in the poultry industry. Huge economic losses occur every year because of mortality and decreased production due to high environmental temperatures. This experiment was conducted to determine the effects of level of inclusion of ascorbic acid supplementation to the diet on performance, carcass composition and bone characteristics of male turkeys during high summer temperature. A total of 120 day-old turkey chicks were wing banded at hatch and randomly distributed into 3 treatment groups. Three diets were formulated to provide a similar nutrient profile with the exception of using three graded levels of ascorbic acid, namely 0, 150 and 300 mg/kg of the diet. The experimental diets were used from 0 to 18 weeks of age. Body weights, feed intake and feed conversion ratio, slaughter weight, carcass yield, portions, composition and thigh and breast pigmentation of the turkeys were not affected by ascorbic acid (p>0.05). Also, shank and tibia bone characteristics were not affected by ascorbic acid (p>0.05).

• BMB Reports
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• v.42 no.11
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• pp.743-746
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• 2009

L-ascorbic acid (Vitamin C) and peptide are both useful compounds for collagen biosynthesis in cosmeceuticals (cosmetic and pharmaceutical fields). The instability of these compounds, however, limit their application in these industries. In this report, we describe the development of a novel compound, Stabilized Ascorbyl Pentapeptide (SAP), which physically is much more stable than L-ascorbic acid in water. The inhibitory effects of this SAP compound on tyrosinase and melanin synthesis is comparable to that of L-ascorbic acid. Importantly, the SAP compound displays no cytotoxicity at a high concentration (5 mM). The ability of SAP to promote collagen biosynthesis is greater than that of L-ascorbic acid or the KTTKS peptide alone. Considering the in vitro stability and functional effects, our data strongly suggest that the SAP compound is a good candidate not only as a cosmetic ingredient, but also as a wound healing agent.

• Journal of the East Asian Society of Dietary Life
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• v.16 no.1
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• pp.107-112
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• 2006

This study analyzed the changes of chlorophyll and ascorbic acid contents according to the extraction temperatures and the number of soakings using five kinds of Korean and Japanese green tea(3 kinds of green tea and 2 kinds of tea bag) which were obtained from local markets in Korea and Japan. The findings are as follows: 1. The chlorophyll contents of Japanese green tea were $2\sim3$ times higher than those of Korean green tea. 2. The chlorophyll contents of Korean green tea in high and middle grades were increased with increasing temperature $(80>70>60^{\circ}C)$ but the contents in low grade were increased with decreasing temperature. The contents of chlorophyll in Japanese green tea were almost similar in low, middle and high grades. 3. Ascorbic acid content was higher in low grade Korean green tea but it was higher in high and middle grades of Japanese green tea. Chlorophyll contents of Japanese green tea were higher than those of Korean green tea but ascorbic acid contents of Korean green tea were higher than those of Japanese green tea.

• YAKHAK HOEJI
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• v.34 no.1
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• pp.22-33
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• 1990

Aqueous solution of bis(2,4-diaminophenyl)phosphonate(APP) was very stable, especially below pH 2.0 and the red-color compound formed by the reaction of APP and $IO_3-$ was stable at room temperature. A simple and rapid spectrophotometric method for the determination of ascorbic acid, potassium antimonyl tartrate (PAT), and isonicotinic acid hydrazide (INAH) was established by the reaction of $IO_3-$ and these reducing drugs, and the absorbance measurements were made at 500 nm. In the reaction of $IO_3-$ and each of the reducing drugs, the conditions of pH were suitable below 2.5 for ascorbic acid, below 2.0 for PAT, and below 1.5 for INAH. Beer's law did hold in the range of $17.6{\sim}1549.9\;ug$ for ascorbic acid, $33.4{\sim}2871.8{\mu}g$ for PAT,and $6.9{\sim}548.6\;{\mu}g$ for INAH. Many common ingredients present in pharmaceutical dosage forms did not interfere. The average recoveries for ascorbic acid and INAH in pharmaceutical formulations were 99.8 $-100.3\;{\pm}\;0.2{\sim}0.4%$, $99.8\;{\pm}\;0.3%$, respectively.

• YAKHAK HOEJI
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• v.47 no.6
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• pp.432-437
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• 2003

It has been known that quercetin, a bioflavonoid widely distributed in fruits and vegetables as dietary-derived flavonoid and exert significant multiple biological effects such as antioxidant and anti-inflammatory, anti-tumor effects. In addition, it has been shown to have a chemoprotective role in cancer, though complex effects on signal transduction involved in cell proliferation and angiogenesis. The present study investigated whether quercetin can enhance antioxidant enzyme activity (glutathione peroxidase: GPx, superoxide dismutase: SOD, catalase: CAT) and regulate the reactive oxygen species (ROS) generation in the presence of vitamin E, L-ascorbic acid, reduced glutathione (GSH) on B16F10 murine melanoma cells. After 48h treatment of cells with quercetin in the presence of vitamin E, L-ascorbic acid, GSH, we measured the cytotoxicities by MTT assay. The cells exhibited a dose-dependent inhibition in their proliferation in the presence of vitamin E, L-ascorbic acid, GSH respectively. We also investigated the effects of antioxidant enzyme activity and ROS generation. The antioxidant enzyme activity of quercetin in the presence of vitamin E was stronger than GSH, L-ascorbic acid, the same treatments decreased ROS generation in B16F10 murine melanoma cells. Taken together, these result demonstrate that the antioxidant effect of quercetin can enhanced in the presence of vitamin E and it might plays an important role in anti-oxidative effects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.4
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• pp.563-569
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• 1995

Myrosinase from Korean cabbage(Bogdoli) was purified and its enzymatic properties were investigated. Myrosinase from the Korean cabbage was purified by DEAE Bio-Gel Sepharose, Concanavalin-A, and Mono-Q column chromatography and exhibited a 55KD molecular weight with a single band on the gel of SDS-PAGE. The enzyme was purified about 21-fold compared to its crude enzyme and a specific activity of purified enzyme was 15, 120units/mg. Optimum pH of the myrosinase was 7.0 in both phosphate and Tris-HCl buffer solutions, the enzyme was stable at pH 6.5~7.0. Optimum temperature of enzyme was 37~38$^{\circ}C$. The enzyme activity was significantly inhibited by Cu2+ and Hg2+, but enhanced by ascorbic acid, resulting in a maximum activity at 1mM ascorbic acid. Among the ascorbic acid analogues, dehydro-ascorbic acid did not affect, whereas others showed a little effect on the enzyme activity, but less than ascorbic acid itself. Reducing agents such as 2-mercaptoethanol and dithiothreitol had no effect on the enzyme activity, but the enzyme activity was enhanced when 2-mercaptoethanol was mixed with ascorbic acid.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.769-773
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• 2007

With the aim to produce ascorbic acid-2-phosphate(AsA-2-P) from L-ascorbic acid(AsA, Vitamin C), nine bacteria conferring the ability to transform AsA to AsA-2-P were isolated from soil samples alongside known strains from culture collections. Most isolates were classified to the genus Brevundimonas by 16S phylogenetic analysis. Among them, Brevundimonas diminuta KACC 10306 was selected as the experimental strain because of its the highest productivity of AsA-2-P. The optimum set of conditions for the AsA-2-P production from AsA using resting cells as the source of the enzyme was also investigated. The optimum cultivation time was 16 h and the cell concentration was 120g/l(wet weight). The optimum concentrations of AsA and pyrophosphate were 550mM and 450mM, respectively. The most effective buffer was 50mM sodium formate. The optimum pH was 4.5 and temperature was $40^{\circ}C$. Under the above conditions, 27.5g/l of AsA-2-P was produced from AsA after 36 h of incubation, which corresponded to a 19.7% conversion efficiency based on the initial concentration of AsA.

• Journal of Nutrition and Health
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• v.17 no.2
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• pp.101-107
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• 1984

Effects of protein-sources on Kimchi fermentation and on the stability of ascorbic acid were investigated at $7{\pm}1^{\circ}C.$ Kimchi samples with various protein sources showed the higher pH and total acidity through the fermentation period than kimchi without the proteins. The results revealed that the proteins have acted as good buffer and lactobacilli-growth enhancer in the fermentation, and the milk proteins showed the most significant effect among the samples. The lactobacilli were at the highest growth at 15th day in all the samples. The amounts and changes in ascorbic acid content during the fermentation did not differ significantly between the control and protein added samples. The fresh, unfermented kimchi contained 14.5-15.7mg of ascorbic acid per 100g of sample and decreased continuously by the 12th day of fermentation. After then the vitamin increased in all the samples and then again decreased slowly after 18th day. The contents of ascorbic acid at the end of the 3 weeks-fermentation(16.3-17.3mg/100g) were still higher than the contents of fresh, unfermented kimchi.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.26 no.1
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• pp.199-212
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• 2000

A series of novel ascorbic acid derivatives, polyethoxylated ascorbic acid (PEAA) were synthesized by coupling ascorbic acid with polyethylene glycol (PEG) of two molecular weights (MW: 350 and 550) at the C-2 or C-3 hydroxyl group (2PEAA350, 3PEAA350, 2PEAA550, 3PEAA550) to increase the stability and retain the activity, as a skin whitening agent. Their stability, scavenging activity against free radical, inhibitory activity against tyroxinase and inhibitory activity of melanin synthesis in Bl6 melanoma cell of PEAAs were evaluated in viかo and compared with those of ascorbic acid and 3-O-ethyl ascorbic acid (3OEAA), a Con stable vitamin C derivative. Among PEAAs, 2PEAA350 and 2PEAA550 tad high scaveniging activity against See radical, inhibitory activity against tyrosinase and inhibitory activity if melanogenesis but low stability, 3PEAA350 had high stability and moderate scavenging activity against free radical, infibitory activity against tyrosinase and inhibitory activity of melanogenesis. The stability, scavenging activity against free radical and inhibitory activity of melanogenesis of 3PEAA350 were higher than those of 30EAA. The most stable 3PEAA350 among PEAAs was nontoxic in various toxicological tests. These results suggest that PEAA would be a good whitening agent far enhancing stability and bioavailability.

• Journal of Food Hygiene and Safety
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• v.15 no.2
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• pp.101-107
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• 2000

This experiment was conducted to investigate the effect of NA formation in the human body with salted and dried yellow corvenia(Gulbi, Pseudosciaena manchurica) steamed for 30 minutes and incubated in 10 ml simulated saliva and 40 ml gastric juice at 37。C for 2 hours. And the experiment studied the NA formation after nitrite, thiocyanate and ascorbic acid treatment under simulated gastric conditions. N-nitrosodimethylamine(NDMA) was not detected when nitrite was not added to the digestate mixture. And then increasing the nitrite concentration from 0.5 mM to 8 mM gave a slight increase in the NDMA formation at all level of Gulbi utilized in the experiment. In contrast to the change of the nitrite concentration, the increase of the amount of Gulbi didn't show any marked influence on NDMA formation. At all level of thiocyanate(1∼6.4 mM) tested, concentration of NDMA was still proportional to the nitrite concentration. In the catalytic ability of thiocyanate at another high level of nitrite(100 mM) no catalytic activity was observed up to the level of 8mM thiocyanate. The same amounts of salted fish(10 g) and ascorbic acid levels in each single digestate were used to prevent NDMA formation. No prevention effect was observed at nitrite concentration of 0.5 mM and 1 mM. Most importantly, the decrease of NDMA concentration, by the increase of ascorbic acid absolutely, was remarkably shown when nitrite concentration was high. On the contrary, according to the degree of ascorbic acid, the higher the amount of nitrite the higher the prevention of NDMA formation.