• Title/Summary/Keyword: artificial medium

Search Result 450, Processing Time 0.071 seconds

An analytical method of soap biodegradability with fatty acid p-BPB derivatives (지방산의 p-BPB 유도체에 의한 비누 생분해도 분석 방법)

  • Oh, Se-Woong;Lee, Ja-Kyoung;Chung, Yong;Chang, Sug-Youn;Kim, Yeo-Kyung
    • Analytical Science and Technology
    • /
    • v.6 no.1
    • /
    • pp.9-19
    • /
    • 1993
  • Fatty acid salts derived from soap can be transferred into a typical derivative with p-bromophenacyl bromide using crown ether, a catalizer by the solid-liquid phase transfer reaction in nonpolar, aprotic solvents and separated by the reverse phase high performance liquid Chromatography (RP-HPLC) and determined using UV detector. The minimal limit of detection was defined at approximately 10~50ng in accordance with the chain length. The derivatization reaction in the presence of EDTA can be applied mot only to the calcium salts but also to the other various metal salts. The recoveries of fatty acid derivatizations in the absence and presence of the midium containing the yeast extract were obtained $95.4{\pm}1.2$, and $85.2{\pm}2.4%$ respectively. The analytical method would be applicable to determine the biodegradation of fatty acid salts in nature as well as in artificial condition such as shaker flask-medium method.

  • PDF

Isolation of a Phenol-degrading Bacterial Strain and Biological Treatment of Wastewater Containing Phenols (Phenol 분해균주의 분리 및 페놀함유 폐수의 생물학적 처리)

  • Lee, Hyun Don;Lee, Myoung Eun;Kim, Hyung Gab;Suh, Hyun-Hyo
    • Journal of Life Science
    • /
    • v.23 no.10
    • /
    • pp.1273-1279
    • /
    • 2013
  • Aromatic hydrocarbons, such as phenol, have been detected frequently in wastewater, soil, and groundwater because of the extensive use of oil products. Bacterial strains (56 isolates) that degraded phenol were isolated from soil and industrial wastewater contaminated with hydrocarbons. GN13, which showed the best cell growth and phenol degradation, was selected for further analysis. The GN13 isolate was identified as Neisseria sp. based on the results of morphological, physiological, and biochemical taxonomic analyses and designated as Neisseria sp. GN13. The optimum temperature and pH for phenol removal of Neisseria sp. GN13 was $32^{\circ}C$ and 7.0, respectively. The highest cell growth occurred after cultivation for 30 hours in a jar fermentor using optimized medium containing 1,000 mg/l of phenol as the sole carbon source. Phenol was not detected after 27 hours of cultivation. Based on the analysis of catechol dioxygenase, it seemed that catechol was degraded through the meta- and ortho-cleavage pathway. Analysis of the biodegradation of phenol by Neisseria sp. GN13 in artificial wastewater containing phenol showed that the removal rate of phenol was 97% during incubation of 30 hours. The removal rate of total organic carbon (TOC) by Neisseria sp. GN13 and activated sludge was 83% and 78%, respectively. The COD removal rate by Neisseria sp. GN13 from petrochemical wastewater was about 1.3 times higher than that of a control containing only activated sludge.

Mycelial characteristics for the artificial cultivation of Inonotus obliqua (Pers.) Pilot (차가버섯(Inonotus obliqua) 인공재배를 위한 균사 배양적 특성)

  • 장현유
    • Korean Journal of Plant Resources
    • /
    • v.15 no.2
    • /
    • pp.135-143
    • /
    • 2002
  • The results of examining characteristics of mycelial growth and culture condition for determining the condition of artificially culturing Inonotus obliqua (chaga) are as follows. 1) Mycelial growth and density oft, oblique were the highest in the medium of BDA (Birch Dextrose Agar; 66.3mm/10d) followed by the order of GDA, PDA, CDA, PODA, ODA, YM, MCM, MEA (pH 7.0), CHA, and MEA (pH 4.7). 2) Optimal temperature for the mycelial growth and density of 1. obliqua were shown to be 30$^{\circ}C$, but the mycelia were dead at 40$^{\circ}C$. the mycelial growth and density of KNAC3005 strains was the highest at 30$^{\circ}C$(66.3mm/10d) followed by the order of 25, 20, 15, 35, 10, and 5$^{\circ}C$. 3) Optimal pH for the mycelial growth and density were revealed to be 6.0 (88.4mm/10d). Above or below pH 6.0, the mycelial growth and density were shown to be retarded. 4) Optimal carbon, nitrogen and organic acid sources for the spawn growth of 40 were maltose (331mg/25$m\ell$/15d), peptone (347mg/25$m\ell$/15d), and glutamic acid (357mg/25$m\ell$/15d), respectively. Optimal level of biotin was 370mg/15d and optimal C/N ratio was 40.

Isolation of Nematophagous Fungi against Root-knot Nematode and Their Growth in Vitro (뿌리혹 선충에 대한 기생 천적 진균 분리 및 이들의 생장에 미치는 환경조사)

  • 정미정;김희규
    • Korean journal of applied entomology
    • /
    • v.27 no.3
    • /
    • pp.149-158
    • /
    • 1988
  • Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

  • PDF

Studies on the Wilt of Strawberry Caused by Fusarium oxysporum f. sp. fragariae in Korea (딸기 시들음병에 관한 연구)

  • Cho Chong Taik;Moon Byung Ju
    • Korean journal of applied entomology
    • /
    • v.23 no.2 s.59
    • /
    • pp.74-81
    • /
    • 1984
  • The experiments were conducted to study the distribution of wilt of strawberry caused by Fusarium in Korea, the characters of the causal fnngus and its control. The results obtained are summarized as follows. 1. Wilt of strawberry has been found in Gimhae and Samrangjin, Gyeongnam province a few years ago. This disease has been spreading year after year, and observed on farms in most of the strawberry-growing areas in Korea. 2. The fungus was isolated frequently from the crowns and petioles of diseased straw berry plants, and the fungus belonging to Fusariun oxysporum in terms of the morphological characteristics of macroconidia, microconidia, chlamydospore and conidiophore on V-8 Agar. 3. The macroconidia formation of the fungus varied remarkably with the Isolates and kinds of medium tested. However. all isolates abundantly produced macroconidia on V-8 Agar. 4. The cross-inoculation tests with several forma specialis of F. oxysporum to cucumber, tomato, watermelon, luffa, cabbage, melon and strawberry were carried out. The isolates from strawberry viz. Kodama's F. oxysporum f. sp. fragariae and S-1 of the authors were pathogenic to only strawberry. The fungus was also similar in morphology and symptoms to Kodama's and Winks' isolate of F. oxysporum f. sp. fragariae. Therefore, the fungus is identified as Fusarium oxysporum Schl. f. sp. fragariae Winks & Williams. 5. The most effective fungicides were Benomyl and Homai for inhibiting sporulation and mycelial growth of the fungus. 6 The cultivar Kurumae 35, Himiko, Senga gigana and Daehak I were resistant, whereas Hokowase, Instiate Z4, Juspa, Puget beauty and Marshall were susceptible to the fungus with artificial inoculation.

  • PDF

'Picnic', a New Mid-season Apple Cultivar with Medium Size and Good Taste (식미가 우수한 중과형 사과 '피크닉' 육성)

  • Kwon, Soon-Il;Park, Jong-Taek;Lee, Jung-Woo;Kim, Mok-Jong;Kim, Jeong-Hee
    • Horticultural Science & Technology
    • /
    • v.33 no.5
    • /
    • pp.784-788
    • /
    • 2015
  • A new cultivar 'Picnic' originated from an artificial cross between 'Fuji' and 'Sansa' at National Institute of Horticultural & Herbal Science in 1994. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2003. After regional adaptability tests in five districts for four subsequent years as 'Wonkyo Ga-34', it was ultimately selected in 2008. Optimum harvest time is late September. Mature fruit has mean weight of 233 g and is conic with light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains a favorable total soluble solids content at $13.8^{\circ}Brix$ and titratable acidity at 0.43%, which results in gustatory harmony between sugars and acids. It is not resistant to bitter rot or Marssonina blotch. 'Picnic' exhibits a physiological cross compatibility with leading cultivars such as 'Fuji', 'Hongro' and 'Tsugaru'. Tree topology is semi-spreading with a weak growth habit.

Changes in Chlorophyll Contents and Photosynthetic Characteristics of Hardwood Species According to Artificial Shade Treatment

  • Choi, Jeong-Ho;Kwon, Ki-Won;Chung, Jin-Chul
    • Journal of Korean Society of Forest Science
    • /
    • v.95 no.5
    • /
    • pp.614-620
    • /
    • 2006
  • To study the chlorophyll contents and photosynthetic characteristics of 4 tree species of deciduous hardwoods; Betula platyphylla var. japonica, Zelkova serrata, Acer mono and Prunus sargentii were treated in 3 stages of shading; the full sun treatment, the medium shade treatment with 30% of transmittance comparing to full sun, the intense shade treatment with 8% of transmittance and their changes in chlorophyll contents and photosynthetic characteristics were examined and analyzed. Most hardwoods showed differences in the total chlorophyll contents in the order of May < September < July, however, that in Prunus sargentii increased progressively along with the lapse of time. Concerning the degree of shading, total chlorophyll contents increased in proportion to the level of shading. Betula platyphylla var. japonica and Prunus sargentii showed more than 2-3 times difference between the full sun treatment and the intense shade treatment. The changes in photosynthetic characteristics, the range of the light saturation point of the trees was $1,000{\sim}1,100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in May, before the shading was applied, and the intensity was shown in the order of Betula platyphylla var. japonica > Zelkova serrata > Acer mono > Prunus sargentii. The photosynthetic rate was $6.4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}{\sim}27.1{\mu}mol{\cdot}CO_2{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of Betula platyphylla var. japonica > Prunus sargentii > Acer mono > Zelkova serrata that there were differences between species. Concerning the changes in light saturation point in each growth period after shading treatment, the light saturation point in the full sun treatment was found in the range of $560{\sim}1,100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and the level of intensity was shown in the order of May > July > September. The light saturation point decreased as the level of shading intensified and the level of changes in light compensation point in the full sun treatment for Betula platyphylla var. japonica and Prunus sargentii was shown in the range of $2.9{\sim}27.1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of May > July > September, however, for Zelkova serrata and Acer mono was shown in the range of $3.9{\sim}11.7{\mu}mol{\cdot}CO_2{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of July > May > September that there were differences between species.

Research and Application of Fault Prediction Method for High-speed EMU Based on PHM Technology (PHM 기술을 이용한 고속 EMU의 고장 예측 방법 연구 및 적용)

  • Wang, Haitao;Min, Byung-Won
    • Journal of Internet of Things and Convergence
    • /
    • v.8 no.6
    • /
    • pp.55-63
    • /
    • 2022
  • In recent years, with the rapid development of large and medium-sized urban rail transit in China, the total operating mileage of high-speed railway and the total number of EMUs(Electric Multiple Units) are rising. The system complexity of high-speed EMU is constantly increasing, which puts forward higher requirements for the safety of equipment and the efficiency of maintenance.At present, the maintenance mode of high-speed EMU in China still adopts the post maintenance method based on planned maintenance and fault maintenance, which leads to insufficient or excessive maintenance, reduces the efficiency of equipment fault handling, and increases the maintenance cost. Based on the intelligent operation and maintenance technology of PHM(prognostics and health management). This thesis builds an integrated PHM platform of "vehicle system-communication system-ground system" by integrating multi-source heterogeneous data of different scenarios of high-speed EMU, and combines the equipment fault mechanism with artificial intelligence algorithms to build a fault prediction model for traction motors of high-speed EMU.Reliable fault prediction and accurate maintenance shall be carried out in advance to ensure safe and efficient operation of high-speed EMU.

A Study on Geospatial Information Role in Digital Twin (디지털트윈에서 공간정보 역할에 관한 연구)

  • Lee, In-Su
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.22 no.3
    • /
    • pp.268-278
    • /
    • 2021
  • Technologies that are leading the fourth industrial revolution, such as the Internet of Things (IoT), big data, artificial intelligence (AI), and cyber-physical systems (CPS) are developing and generalizing. The demand to improve productivity, economy, safety, etc., is spreading in various industrial fields by applying these technologies. Digital twins are attracting attention as an important technology trend to meet demands and is one of the top 10 tasks of the Korean version of the New Deal. In this study, papers, magazines, reports, and other literature were searched using Google. In order to investigate the contribution or role of geospatial information in the digital twin application, the definition of a digital twin, we investigated technology trends of domestic and foreign companies; the components of digital twins required in manufacturing, plants, and smart cities; and the core techniques for driving a digital twin. In addition, the contributing contents of geospatial information were summarized by searching for a sentence or word linked between geospatial-related keywords (i.e., Geospatial Information, Geospatial data, Location, Map, and Geodata and Digital Twin). As a result of the survey, Geospatial information is not only providing a role as a medium connecting objects, things, people, processes, data, and products, but also providing reliable decision-making support, linkage fusion, location information provision, and frameworks. It was found that it can contribute to maximizing the value of utilization of digital twins.

A Study on Operation Control Technology Required for Introduction of Intelligent Sewage Treatment Plant (스마트 하수처리장 도입에 필요한 운전제어기술에 관한 연구)

  • Lee, Jiwon;Kim, Yuhyeon;Gil, Kyungik
    • Journal of Wetlands Research
    • /
    • v.24 no.1
    • /
    • pp.38-43
    • /
    • 2022
  • Smart sewage treatment plant means creating a safe and clean water environment by establishing an ICT-based real-time monitoring, remote control management and intelligent system for the entire sewage treatment process. The core technology of such a smart sewage treatment plant can be operation control technology using measuring instruments. This research team analyzed and suggested the operation control technologies necessary for the establishment of the intelligent business by referring to the intelligent research projects of the sewage treatment plant in progress in Korea. As a result of the analysis, a total of six removal technologies were presented, including control by scale, reflow water control, linked treated water control, chemical quantity control, winter operation control, and total organic carbon control. By size, standards that can be classified into small and medium-sized large-scale are presented, and in the case of reflow water control, the location of water quality and flow sensors capable of managing reflow water is suggested. In the case of the linked treated water control, the influence and control points of the linked treated water on the sewage treatment plant were presented, and in the case of the chemical injection volume control, a system capable of optimizing the amount of chemical injection according to the introduction of an intelligent sewage treatment plant was presented. In the case of winter operation, the sensors and pumps to be controlled are suggested when considering the decrease in nitrification due to the decrease in water temperature. In the case of total organic carbon control, an interlocking system considering the total amount of pollution in the future was proposed. These operation control scenarios are expected to be used as basic data to be used in intelligent sewage treatment algorithms and scenarios in the future.