• Biotechnology and Bioprocess Engineering:BBE
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• 제7권4호
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• pp.206-211
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• 2002

Calli obtained from a shoot-tip of garlic, Allium sntivum L., were encapsulated using a calcium alginate gel. Some of the encapsulated calli were cultured on a 1/2 MS medium supplemented with 3% sucrose, 10$\^$-5/ kinetin, and 5 ${\times}$ 10$\^$-6/ M NAA whereas the remainder was stored for 40 days at 4$^{\circ}C$. All the naked calli regenerated on the solid medium, while 95% of the encapsulated calli regenerated, and 88% of the encapsulated calli regenerated after 40 days of storage at 4$^{\circ}C$. The capsule matrix delayed the germination time of the encapsulated calli, yet activated the shoot formation of the artificial garlic seeds. The shoot length of the encapsulated garlic calli was much longer than that of the naked garlic calli. The encapsulated garlic calli were dried in a laminar airflow cabinet and the conversion frequency of the dried artificial garlic seeds on a 1/2 MS medium remained at 93% with a water Loss of Less than 50%.

• 생물환경조절학회지
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• 제27권1호
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• pp.71-79
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• 2018

본 연구는 우량묘 생산을 위해 신개발된 인공배지와 육묘용 배지의 이화학적 특성을 비교하고 적정 관수 간격을 구명하기 위해 수행되었다. 5종의 기존 인공배지(유기배지인 coir, 혼합배지인 Tosilee와 Q plug, 그리고 무기배지인 LC와 rockwool)와 4종의 신개발 배지(혼합배지인 TP-S1, 그리고 무기배지인 PU 14-S1, PU-7B, PU 15-S1)를 이용하여 토마토(Solanum lycopersicum L.) '예광'을 육묘하였으며, 14일간 1일(14회), 2일(7회) 그리고 3일(5회) 간격으로 관수 처리하였다. pH는 PU 15-S1 배지에서 유의성 있게 가장 높았으며, 모든 배지에서 pH 5.17-6.90의 범위였다. EC는 Q plug 배지에서 가장 높게 나타났다. 초기발아율은 PU 15-S1 배지에서 가장 우수하였다. 최종발아율과 평균발아수는 PU 14-S1 배지를 제외하고는 모든 배지에서 유의적인 차이가 없었다. 파종 후 15일째 토마토 묘의 생육은 Q plug 배지에서 우수하였다. 파종 후 29일 째 묘의 생육 또한 Q plug 배지에서 유의적으로 우수하게 나타났고, 다음으로 rockwool과 PU-7B 배지에서 우수하였다. 또한 생육은 1일 간격 처리에서 가장 우수한 경향을 보였다. 결과적으로 본 실험에서 토마토 육묘 시 인공배지의 적용가능성을 확인하였으며, 묘의 생육은 Q plug 배지에서 가장 우수하였다. 신개발된 PU-7B 인공배지에 양분을 첨가하고 1일 간격으로 관수한다면 Q plug 배지의 결과와 같이 우수한 토마토 묘를 생산할 수 있을 것으로 판단된다.

• 한국통신학회논문지
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• 제33권10C호
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• pp.844-850
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• 2008

본 논문에서는 지하 암반을 타고 전달된 인공발파음 특성을 규명하기 위해 제안한 알고리즘에 대해 기술한다. 지하 암반 매질을 통과한 인공발파음은 다중전달경로 현상과 지질의 불균일 등으로 인해서 거리증가에 따라 고주파 대역에서 감쇠가 발생한다. 본 논문에서는 제안한 알고리즘 성능검증을 위해 지하터널에서 발파 실험을 하였고 수집한 데이터를 가지고 지하암반을 통과한 채널에서 특징 파라미터를 추출하여 수치적으로 정량화함으로써 인공발파음 특성을 규명하였다.

• Mycobiology
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• 제35권4호
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• pp.226-229
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• 2007

To produce fruiting bodies of Oudemansiella mucida, porcelain fungus, on the oak sawdust medium, additives suitable for the mycelial growth and fruiting body formation were screened. In general, the mycelial growth of the three strains of O. mucida used in this study have been good on oak sawdust mixed rice bran of $20{\sim}30%$. The mycelia incubated in potato dextrose broth for 7 days were inoculated on oak sawdust medium supplemented with various ratios of rice bran and incubated for 30 days at $25^{\circ}C$ in the dark condition until the mycelia of O. mucida fully colonized the media from top to bottom. Then, top surface of the media in the bottles were horizontally scratched with a spatula and filled with tap water for 3 hours. To induce the primordial formation of O. mucida, the bottles were transferred to the mushroom cultivating room under 12 hrs of light (350 lux) and dark condition with relative humidity of 95% at $17^{\circ}C$. The primordia of O. mucida were formed on the surface of oak sawdust media after 7 days of incubation. The mature fruiting bodies were observed 5 days after primordial formation. The fruiting bodies O. mucida were formed on oak sawdust medium mixed with 5 to 30% rice bran. However, abundant fruiting-bodies of O. mucida were produced in oak sawdust medium supplemented with 20% rice bran. This is the first report associated with an artificial fruiting body production of O. mucida in Korea.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.659-659
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• 2003

In this study, we have investigated the potent anti-aging effect of DA-3711, a cosmetic ingredient derived from artificial skin culture. The artificial skin was originally developed as a skin replacement for the treatment of chronic skin wounds. To produce DA-3711, neonatal human fibroblasts were seeded into biocompatible collagen/chitosan/glycosaminoglycan (GAG) scaffolds and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with fetal bovine serum and nonessential amino acids. Analysis of the culture broth (DA-3711) showed that growth factors such as VEGF, TGF-$\beta$, KGF were present at significantly higher levels that in the culture broth of fibroblasts cultured in monolayer. The biological activity of DA-3711 was assessed by measuring in vitro cell proliferation and collagen synthesis of normal human fibroblasts. Fibroblasts treated with 10％ DA-3711 showed a 2-fold higher proliferation and 2 to 4-fold higher collagen synthesis than untreated cells. DA-3711 also exhibited anti-oxidative effects, since cells under peroxide-induced oxidative stress showed a 30％ higher viability in DA-3711-containing medium than in medium without DA-3711 addition. The results suggest that DA-3711 may have anti-aging effects by stimulating skin regeneration and protecting against oxidative stress.

• 융합신호처리학회논문지
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• 제23권2호
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• pp.50-61
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• 2022

This work demonstrates the design and evaluation of Aqua-Aware, a lightweight miniaturized light emitting diode (LED) based underwater compact sensor node which is used to obtain different characteristics of the underwater environment. Two optical sensor nodes have been designed, developed, and evaluated for a short and medium link range called as Aqua-Aware short range (AASR) and Aqua-Aware medium range (AAMR), respectively. The hardware and software implementation of proposed sensor node, algorithms, and trade-offs have been discussed in this paper. The underwater environment is emulated by introducing different turbulence effects such as air bubbles, waves and turbidity in a 4-m water tank. In clear water, the Aqua-Aware achieved a data rate of 0.2 Mbps at communication link up to 2-m. The Aqua-Aware was able to achieve 0.2 Mbps in a turbid water of 64 NTU in the presence of moderate water waves and air bubbles within the communication link range of 1.7-m. We have evaluated the luminous intensity, packet success rate and bit error rate performance of the proposed system obtained by varying the various medium characteristics.

• Mycobiology
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• 제28권4호
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• pp.165-170
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• 2000

This study was carried out to obtain the basic data for artificial culture of veiled lady mushroom (Dictyophora spp). The optimal conditions for the mycelial growth were $25^{\circ}C$ and pH 5.0 for all isolates except the optimal temperature of $30^{\circ}C$ for D. echinovolvata ASI 32002 and Phallus rugulosus. The optimal medium for Dictyophora spp. was PBA (potato bamboo sawdust extract agar) medium. The strain ASI 32002, D. echinovolvata, grew faster than. D. indusiata ASI 32003 and Phallus rugulosus ASI 25007 on the medium. Carbon sources such as glucose, maltose and inuline were favorable for stimulating a mycelial growth of the two strains of ASI 32002 and ASI 32003. Asparagine and glutamine appeared to be favorable to the strain ASI 32002 and ASI 32003, where as alanine, one of nitrogen source also favorable to the strain ASI 32002. The optimum C/N ratio of the two isolates of ASI 32002 and ASI 32003 was about 25 : 1 when 2% glucose as carbon source was mixed with the basal medium. While, in the case of 4% as carbon source, the optimum C/N ratio was about 30 : 1.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.700-718
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• 2003

Fructan, a polysaccharide existing in plants or produced by microorganisms, is a sugar polymer of fructose with $\beta$-2,6 linkages. In this study, we investigated some cosmeceutical properties of Fructan such as moisturizing effect, cell proliferation effect, anti-inflammation effect and cell cytotoxicity. Zymomonas mobilis, a microorganism producing Fructan, was cultured in a medium containing 10％ sucrose and 2％ yeast extract as main components for 24 hours at 37$^{\circ}C$ and pH 7. Fructan was obtained by precipitation from the cultured medium by adding alcohol (alcohol ratio of 1:3) after removing the enzyme by centrifuging. Fructan exhibited almost same moisturizing effect as hyaluronic acid and cell proliferation effect on human fibroblast and keratinocyte as well. Moreover, on cell proliferation test on bio-artificial skin constructed by 3-dimensional(3-D) culture after inducing primary skin inflammation with 0.5％ sodium lauryl sulfate (SLS), the 3-D artificial skin treated with 0.01 mg/ml, 0.05mg/ml of Fructan exhibited higher cell proliferation than the 3-D artificial skin treated with SLS only. On anti-inflammation test on 3-D artificial skin evaluated by measuring secreted quantity of interleukin-1$\alpha$ (IL-1$\alpha$) which is a pre-inflammatory mediator induced by SLS, the quantity of IL-1$\alpha$on the 3-D artificial skin treated with 0.01 mg/ml, 0.05mg/ml of Fructan was less than the one on the 3-D artificial skin treated with SLS only. As a result of these studies, Fructan has anti-inflammation effect against inflammatory reaction by a skin irritant as well as cell proliferation effect in bio-artificial skin. Fructan was also evaluated as a safe material without any toxicity in safety tests using fibroblasts and animals.

• Journal of Microbiology and Biotechnology
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• 제2권1호
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• pp.1-6
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• 1992

The aspartame, $\alpha$-L-aspartyl-L-phenylalanine methylester, is an artificial sweetener. Taking advantage of the fact that the aspartame is a derivative of dipeptide, synthesis of aspartame from the artificial polypeptide made by an artificial gene has been attempted. The artificial polypeptide (LAP32), a polymer of tripeptide (aspartyl-phenylalanyl-lysine), was purified from the E. coli cells harboring a recombinant plasmid containing the artificial gene. This polypeptide was then digested with trypsin and carboxypeptidase B to produce dipeptide (Asp-Phe). Using the esterase activity of $\alpha$-chymotrypsin, the dipeptide was directly converted into Asp-Phe methylester in a water-methanol system. When the methanol concentration in reaction mixture was 25%, 50% of dipeptide was converted to the dipeptide methylester without producing any by-products.

• 한국조경학회지
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• 제29권5호
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• pp.20-27
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• 2001

The purpose of this study is to investigate and compare the validity and the reliability of the visual simulation method using the internet. For this. the evaluation of the artificial and natural landscape through the medium of color slides are compared with the internet survey. Data is analysed through the comparison of t-test between the two media by landscape type, and spatial image is analysed by factor analysis algorithm. Principle component analysis using Varimax Method is applied for extraction and factor rotation respectively. The results of this study can be summarized as follows; There are no statistical differences between the two methods with artificial and natural landscape in the total data that included second tests. Factors covering the spatial image are found to be \`aesthetic\`, \`spatial shape\`, and \`familiarity\`. Total variance is obtained as 66.4%. There are no statistical differences between the two methods in 2/3 of the cases. In the case of far view of artificial landscape, the results of the t-test show that the two methods are exactly the same. Especially in the case of the artificial far landscape shows no difference of all factors between two methods. There are no differences between first and second tests of the same media and the same landscape type. And it shows the reliability of this method. These results suggest that the probability that the internet can be used as a medium of landscape evaluation and gathering information on anyone\`s landscape image. Simulation techniques with the internet survey method should be further developed for practical application.