• The Korean Journal of Pesticide Science
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• v.20 no.2
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• pp.83-92
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• 2016

New tree wound-treatment formulations (WTF) were developed in this study. Stimulating effects of plant growth regulating substance on callus formation were evaluated in vivo twig disc culture of Zelkova serrata, Ziziphus jujuba, Pinus densiflora, Ginkgo biloba. and Aesculus turbinata. Based on the evaluation, WTF were prepared with 2,4-D, NAA, and IBA using xanthan gum as an extending agent. WTF were pasted on $2{\times}10cm$ artificial wound on the stem and the effects were evaluated 12 weeks later with the thickness of new callus-tissues. The effects varied with tree species and the growth regulators. In Z. serrata and A. turbinata, 2 mg/L of 2,4-D was the best WTF. In P. koraiensis and G. biloba, 8 mg/L of NAA and 1 mg/L of IBA were the best, respectively. Callus formation was quite lower in G. biloba compared to other species. Stimulating effect of thiophanate-methyl paste was excellent only in Z. serrata and lower than certain WTF in other species. Vaseline, which was used generally as an WTF, remained longer on the wound and causing decay. From the results, it could be recommended that 2,4-D, NAA and IBA were the best WTF for Z. serrata/A. turbinata, P. koraiensis and G. biloba, respectively.

• Journal of Convergence for Information Technology
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• v.8 no.1
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• pp.235-242
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• 2018

The interest about 4th Industrial Revolution was impressively increased from newspapers, iindustry, government and academic sectors. Especially AI what could be felt by the skin of many peoples, already overpassed the ability of the human's even in creative areas. Namely, now many people start fo feel that the effect of the revolution is just infront of themselves. There were several issues in this trend, the ability of deep learning by machine, the identity of the human, the change of job environment and the concern about the social change etc. Recently many studies have been made about the 4th industrial revolution in many fields like as AI(artificial intelligence), CRISPR, big data and driverless car etc. As many positive effects and pessimistic effects are existed at the same time and many preventing actions are being suggested recently, these opinions will be compared and analyzed and better solutions will be found eventually. Several educational, political, scientific, social and ethical effects and solutions were studied and suggested in this study. Clear implication from the study is that the world we will live from now on is changing faster than ever in the social, industrial, political and educational environment. If it will reform the social systems according to those changes, a society (nation or government) will grasp the chance of its development or take-off, otherwise, it will consume the resources ineffectively and lose the competition as a whole society. But the method of that reform is not that apparent in many aspects as the revolution is progressing currently and its definition should be made whether in industrial or scientific aspect. The person or nation who will define it will have the advantage of leading the future of that business or society.

• The Korean Journal of Mycology
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• v.22 no.2
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• pp.145-152
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• 1994

In order to investigate the cultural characteristics of Lentinus lepideus, media, pH, carbon sources, nitrogen sources, vitamin and organic acids were tested in submerged culture. It grew well in GPB(broth), and the optimum temperature and pH was $25^{\circ}C$ and 4.2, respectively. The carbon sources such as galactose belong to monosaccharide and maltose belong to disaccharide were effective for mycelial growth. Peptone which is compound nitrogen source was good for mycelial yield but the other nitrogen sources were not effective. Among the various vitamins, pyridoxine is suitable for mycelial yield. Among the various organic acids, citric acid promoted the mycelial yield but acetic acid interrupted the mycelial growth of L. lepideus.

• Korean Journal of Ichthyology
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• v.12 no.1
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• pp.46-53
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• 2000

Artificial gonadal maturation and spawning inducement of striped knife-jaw Oplegnathus fasciatus were studied. Effects of water temperature and photoperiod under different regims on gonadal activity and maturation of three years old O. fasciatus were investigated histologically. In experiment I (Exp. I), water temperature was gradually increased from $14.5^{\circ}C$ to $21.0^{\circ}C$ and photoperiod was also gradually increased from 10 : 30 L to 15 : 30 L from December 1996 to February 1997 and this conditions were maintained till April. In experiment II (Exp. II), water temperature was increased in the same way from Exp. I and photoperiod was controlled as natural condition till early March and then increased to 15 : 30 L immediately. Control fish were reared in net-cage culture system in the sea from December 1996 to April 1997. Gonadal activity was initiated by increasing water temperature in both Exp. I and II from January. In Exp. I, gonadal maturation and spawning were induced from February when water temperature and photoperiod reached at $21.0^{\circ}C$ and 15 : 30 L, respectively. In Exp. II, complete gonadal maturation was not induced until early March but after treated by compensatory long photoperiod (15 : 30 L), the gonad was matured and subsequently spawning occurred.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.333-342
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• 2010

For the energy-saving production of fresh vegetables in poor environment such as the Antarctic, a container-type plant factory was designed and developed. To maximize space usage of the 20 feet container ($L5.9m{\times}W2.4m{\times}H2.4m$), a three-level hydroponic cultivation system was installed and the nutrient solution was supplied by bottom watering. Using this system, 3 lettuce cultivars were grown under different the light source (light intensity). After 2 weeks from the transplanting, fluorescent lamp ($145\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) showed the best fresh weight of top part and leaf area. However, After 4 weeks, fluorescent lamp plus metal halide lamp ($150\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) led to the optimum growth of the each lettuce cultivar. The cultivar, 'Cheongchima', showed the best fresh weight of top part and leaf area, followed by 'Jeokchukmyeon' and 'Lollo rosa'. The chlorophyll concentrations (SPAD) showed no significant difference among the sources of lights. However, 'Cheongchima' showed relatively high chlorophyll concentration. With the above results, we found that the growth of lettuce is depend on light intensity and even at same intensity, the growth is different among the cultivars. Therefore, the selection of optimum cultivar should be considered in the plant factory system that has only weak light density.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.297-300
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• 2017

Plant factory can control artificially the environments for crop cultivation, so they can produce high quality agricultural products all year round. This study was carried to select suitable kohlrabi cultivar for hydroponics in a closed-type plant factory system. We used three cultivars of red kohlrabi, 'Asac kohl', 'Kolibri', and 'Purple king' as plant materials. The artificial light source was LED light, light intensity and photoperiod were $249{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and 12/12 hours (day/night period), respectively. Hydroponic cultivation type was used circulating deep flow technique. At 43 days after transplanting, fresh weight of whole plant and tuber and leaf area were not significantly different among cultivars. Shoot dry weight and tuber dry weight were highest in 'Asac kohl' cultivar, and number of leaves was highest in 'Purple king' cultivar. Sugar content and yield were highest in 'Asac kohl' cultivar. Considering the growth and marketable yields, 'Asac kohl' was the optimal kohlrabi cultivar for hydroponic cultivation in a closed-type plant factory system.

• Journal of Mushroom
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• v.5 no.2
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• pp.76-80
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• 2007

After bottle culture of Pleurotus eryngii, media were taken out the bottle and normally utilized compost. However, nutritional elements were remained in the waste media. This study was carried out to investigate the reusable possibility and the optimal additive rate of waste media in an artificial cultivation of Pleurotus eryngii. The pH had tendency to decrease as the waste media was added from 6.0 to 4.8. Based on the additive rate of 10, 20, 30, 40, and 50%, each treatment waste media was added to new media for culturing Pleurotus eryngii. Among various treatments, the mycelial growth and primordia formation of Pleurotus eryngii were more favorable in the addition of 10-30% waste median than in the addition of 40 and 50%. The yield of its fruiting body was increased slightly in the treatment of 10-30% waste media.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.102-109
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• 2012

In this study, the four different filter media (sponge, volcanic stone, activated carbon and magnesium hydroxide) were tested for the removal of algae and phosphorus in the two eutrophic water samples (natural water and artificial algal culture with BG-11 medium). These filter media were used in the column device as single or combined applications. The effect of the $Mg(OH)_2$ on phosphorus removal was examined using different particle sizes (<2 mm and >2 mm) and concentrations (0, 10, 50 and 100 g $L^{-1}$) of magnesium hydroxide. The removal efficiency of phosphate by magnesium hydroxide was increased with longer experimental time and higher concentration. However, there was no significant difference in the degree of phosphorus removal between any two particle sizes (1 mg P $L^{-1}$: F=0.109, P=0.685; 10 mg P $L^{-1}$: F=1.542, P=0.355). Among the four media, activated carbon showed the most potent effect on the removal of both algae and phosphorus. The highest removal efficiency of algae and phosphorus was obtained by combining four columns of each filter medium. Interestingly, integration of four filter columns showed higher removal efficiency than activated carbon alone. The highest removal efficiency by integrated filter columns seemed to be caused by a synergistic effect of combined activated carbon and magnesium hydroxide.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.5
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• pp.749-755
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• 2014

Exopolysaccharides (EPSs) have been widely used in the food industry as viscofying, stabilizing, and emulsifying agents as well as in the pharmaceutical industry for their immunomodulatory, anti-tumor, and anti-inflammatory effects. A total of 458 lactic acid bacteria (LAB) strains isolated from several kinds of soybean pastes were screened for the production of homo-EPS (HoPS). LAB isolates were primarily screened using thin layer chromatography (TLC) and further screened polymerase chain reaction (PCR) targeting genes involved in HoPS production. Six LAB isolates producing high amounts of HoPS were identified by TLC. Among these isolates, glucansucrase gene was amplified in two strains (JSA57, JSB22), whereas the fructansucrase gene was detected in three strains (JSA57, JSB22, JSB66). After isolating the strains, their morphological characteristics and 16S rDNA sequences were determined. Six species were identified as L. alimentarius HSB15, L. plantarum JSA22, L. pentosus JSA57, L. brevis JSB22, L. alimentarius JSB66, and L. parabrevis JSB89. To evaluate the potential probiotic properties of these LAB, their survival rates against a simulated intestinal environment were determined. After 2 hr of incubation in artificial gastric juice, survival rates of JSA57, JSB90, JSB22, and JSB66 were all greater than 50%. After 2 hr of incubation in bile juice, viable cell count of JSB22 was similar with initial vial cell counts. Growth of the six LAB was screened in arabino-oligosaccharide (AOS)-containing MRS broth. Results showed that growth of the isolates selectively increased after culture in AOS-containing media. Strain JSB22 (6 hr), JSB66 (6 hr), HSB15 (20 hr), and JSA22 (29 hr) showed maximum growth rate. Especially, JSB22 showed the highest growth rate. These results suggest that EPS-producing LAB isolated from Deonjang could be applied as synbiotics.

• Journal of Mushroom
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• v.3 no.2
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• pp.45-51
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• 2005

This study was carried out to obtain the basic data on artificial culture of Sparassis crispa. The mycelial growth and density were good in the PBA, PDA and PDM media, and especially prominent in the PBA medium. The optimum condition for the mycelial growth was $25^{\circ}C$ and pH 5.0, respectively. The carbon sources such as glucose, fructose, mannose, xylose(monosaccharide) and maltose(disaccharide) were favorable to mycelial growth of S crispa. The optimal concentration of glucose is 1.0~1.5%. As nitrogen sources, threonine, peptone, glycine, glutamine and valine appeared to be favorable to the mycelial growth and density. The optimal concentration of peptone is 0.3%.