• Journal of Aquaculture
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• v.13 no.1
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• pp.29-37
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• 2000

In previously study we isolated several fish matrix attachment regions (MARs) capable of replicating the plasmid by itself. In this study we construct a fish expression vector pBaEGFP(+) containing mud loach ${\beta}$-actin promoter EGFP as reporter gene and SV40 signal. To analyze the effects of the fish expression vector respectively. The fish ARS containing constructs pBaEGFP(+)-ARSs were transfected cells with pBaEGFP(+)-ARS101 and pBaEGFP(+)-ARS223 reduced 10 days to 25 days and then was constant to 30 days after transfection while that of the control vector without ARS element was basal level. The intensity of both constructs showed about 30fold of the intensity compared with the control vector on 30days after transfection individually .E. coli back-transformation analysis shows that pBaEGFP(+)-ARS223 and pBaEGFP(+)-ARS905 maintain in episomal state at least 30 days after transfection. The result indicates that both may be able to replicate the vector in BF-2 cell. Therefore the matrix-attached ARSs enhancing expression of the reporter gene might be useful as a component o the expression vector for transgenic studies.

• Korean Journal of Clinical Pharmacy
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• v.26 no.1
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• pp.40-45
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• 2016

Objective: The increasing use of imaging examinations such as computed tomography (CT) results in increased contrast media use, which increases contrast media-induced adverse reactions (AR). This study investigated the risk factors of ARs to nonionic iodinated contrast media. Methods: This study evaluated patients who were administered iodinated contrast media during CT scanning in Yeouido St. Mary's Hospital in Seoul, Korea in 2012. Among the subjects, those with contrast media-induced ARs were classified as the AR group. The control group included individuals without ARs who were selected through simple random sampling. The effects of sex, age, contrast media type and dose, CT region, previous contrast media administration, allergy history, and comorbidity were analyzed in the AR and control groups. Results: Multivariate logistic regression analyses were performed to evaluate the identified AR risk factors in 103 subjects in the AR group and 412 subjects in the control group. The results confirmed that the risk of developing ARs was significantly higher in females [odds ratio (OR): 2.206; 95% confidence interval (CI): 1.353-3.598], in individuals administered Iohexol (OR: 9.981; 95% CI: 2.361-42.193), in individuals with an allergy history (OR: 3.982; 95% CI: 1.742-9.101), and in individuals with comorbid asthma (OR: 6.619; 95% CI: 1.377-31.826). Most of the ARs were mild and immediate. Conclusion: In patients who were administered contrast media during CT scans, female gender, Iohexol use, allergy history, and asthma were risk factors for ARs. Therefore, special care is required for patients with such risk factors to prevent ARs.

• Journal of Microbiology and Biotechnology
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• v.3 no.1
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• pp.6-11
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• 1993

An autonomously replicating sequence (Kf-ARS1) of Kluyveromyces fragilis was cloned from the genomic library which was constructed using pHN134 as a cloning vector to make a new host-vector system for the production of heterologous protein from K. fragilis as a host. The cloning vector pHN134 was composed of $Km^r, Ap^r$ and multiple cloning site in LacZ . A clone carrying Kf-ARS1 was isolated and the recombinant plasmid was designated as pIKD102. The cloned fragment was 2.3 kb (EcoRI/EcoRI) in length. Subcloning experiment showed that the region for ARS activity was 1.5 kb (SalI/EcoRI) fragment. It was shown that the Kf-ARS1 was active in Saccharomyces cerevisiae and Kluyveromyces fragilis.

• Journal of Advanced Marine Engineering and Technology
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• v.32 no.1
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• pp.200-205
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• 2008

Stability and durability of the artificial reefs (ARs) installed for wildstock enhancement have been a key issue. We surveyed the stability of 4,044 artificial reefs installed at 5 sites (Gangeung, Donghae, Samcheok, Goseong. and Yangyang) along the Gangwon coast of the East Sea (Sea of Japan) through which 1 to 5 typhoons pass annually. The ARs surveyed were 7 types aging 1 to 4 years. The side scan sonar (SSS) and scuba diving surveys confirmed that the ARs stability was age and type-dependent. A total of 85.56% ARs remained safe. the rest 14.44% being influenced by physical parameters of the waters such as wave extremes mostly caused by typhoons. An interesting observation was that the AR were particulatly vulnerable to the wave extremes in the first year of installation, thereafter being less damageable. Representative damages of the ARs were capsized (0.20%). broken (5.79%), sunk (0.27%), and buried (8.18%). By type, dice reef was most stable. while semicircle branch reef was most vulnerable.

• Asian-Australasian Journal of Animal Sciences
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• v.1 no.1
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• pp.1-5
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• 1988

Ninety feeder steers, predominantly Herefords weighing 280 kg, were assigned at random to each of nine diets: basal (high concentrate); 25 or 50% of untreated rice straw (25- or 50 URS) or ammoniated rice straw (25- or 50 ARS); a 50:50 mixture of URS - alfalfa or ARS - alfalfa replacing a proportion of the basal at 25 and 50%. Animals were slaughtered after 154 or 161 days of feeding. The cattle fed 50% URS had the lowest (P<.05) carcass weight, dressing & external fat thickness, kidney, pelvic and heart fat, rib eye area, marbling score, quality and yield grades, followed by cattle fed 50% ARS, 50% rice straw/alfalfa, and 25% rice straw alone or 25% rice straw/alfalfa mixture. Ammoniation of rice straw improved all measured traits. A 25% substitution of basal diet with untreated or ammoniated rice straw and a 50% substitution with rice straw/alfalfa mixture did not significantly affect carcass traits compared to the basal group. Only 50% ARS and 50% URS cattle showed differences in body composition (P<.05) with lower fat and higher water and protein contents. No significant differences were found in shear value, panel tenderness, connective tissue, juiciness, flavor and overall palatability of meat from steers fed the basal, ARS or URS diets.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.11
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• pp.1541-1546
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• 2016

Four carcass traits, namely carcass weight (CW), eye muscle area (EMA), back fat thickness (BF), and marbling score (MS), are the main price decision parameters used for purchasing Hanwoo beef. The development of DNA markers for these carcass traits for use in a beef management system could result in substantial profit for beef producers in Korea. The objective of this study was to validate the association of highly significant single nucleotide polymorphisms (SNPs) identified in a previous genome-wide association study (GWAS) with the four carcass traits in a commercial Hanwoo population. We genotyped 83 SNPs distributed across all 29 autosomes in 867 steers from a Korean Hanwoo feedlot. Six SNPs, namely ARS-BFGL-NGS-22774 (Chr4, Pos:4889229), ARS-BFGL-NGS-100046 (Chr6, Pos:61917424), ARS-BFGL-NGS-39006 (Chr27, Pos:38059196), ARS-BFGL-NGS-18790 (Chr10, Pos:26489109), ARS-BFGL-NGS-43879 (Chr9, Pos:39964297), and BTB-00775794 (Chr20, Pos:20476265), were found to be associated with CW, EMA, BF, and MS. The ARS-BFGL-NGS-22774, BTB-00775794, and ARS-BFGL-NGS-39006 markers accounted for 1.80%, 1.72%, and 1.35% (p<0.01), respectively, of the phenotypic variance in the commercial Hanwoo population. Many genes located in close proximity to the significant SNPs identified in this study were previously reported to have roles in carcass traits. The results of this study could be useful for marker-assisted selection programs.

• Journal of Advanced Marine Engineering and Technology
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• v.34 no.1
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• pp.168-176
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• 2010

Stability and durability of the artificial reefs (ARs) for wildstock enhancement have been a key issue. We surveyed the side scan sonar (SSS), multi beam echo sounder (MBES) and scuba diving surveys confirmed that the ARs stability was age and type-dependent. A case of the stability of 8,438 ARs (60 group ARs) installed at 4 sites (Yangyang, Gangeung, Donghae and Samcheok) along the Gangwon coast of the East Sea (Sea of Japan). And a confirmation amount to 85.4% ARs remained safe, the rest 14.6% being influenced by a physical parameter of the waters. The representative influences by their circumstances were of breakage (6.3%). capsizing (8.3%). A group of a ARs to 61.7% ARs remained safe, the rest 38.3% being influenced by a physical parameter of the waters. The representative influences by their circumstances were of buried.

• Journal of The Korean Astronomical Society
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• v.47 no.3
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• pp.105-113
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• 2014

We apply differential affine velocity estimator (DAVE) to the Solar Dynamics Observatory (SDO)/Helioseismic and Magnetic Imager (HMI) 12-min line-of-sight magnetograms, and separately calculate the injected magnetic helicity for the leading and the following polarities of nine emerging bipolar active regions (ARs). Comparing magnetic helicity flux of the leading polarity with the following polarity, we find that six ARs studied in this paper have the following polarity that injected more magnetic helicity flux than that of the leading polarity. We also measure the mean area of each polarity in all the nine ARs, and find that the compact polarity tend to possess more magnetic helicity flux than the fragmented one. Our results confirm the previous studies on asymmetry of magnetic helicity that emerging bipolar ARs have a polarity preference in injecting magnetic helicity. Based on the changes of unsigned magnetic flux, we divide the emergence process into two evolutionary stages: (1) an increasing stage before the peak flux and (2) a constant or decreasing stage after the peak flux. Obvious changes on magnetic helicity flux can be seen during transition from one stage to another. Seven ARs have one or both polarity that changed the sign of magnetic helicity flux. Additionally, the prevailing polarity of the two ARs, which injects more magnetic helicity, changes form the following polarity to the leading one.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.4
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• pp.490-496
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• 2000

In vivo digestibility, nitrogen retention and microbial protein yield from diets of 100% ammonia treated rice straw (ARS) ($D_1$); 65% untreated rice straw (URS)+30% rice bran (RB)+5% SBM ($D_2$) and 85% ARS+15% RB ($D_3$) were determined using three Japanese Corriedale wethers in a $3{\times}3$ Latin Square Design. Results showed that DM consumption and organic matter digestibility were highest in $D_3$; but did not promote high protein digestibility, which RB+SBM had effected in URS based-diet. Dry matter intake and OM digestibility were the same for $D_1$ and $D_3$. Solubility of fiber bonds was increased by ammoniation, resulting in higher NDF digestibility. Nitrogen retention and microbial protein yield of rice bran supplemented groups was higher than ARS, but supplementation did not significantly increase efficiency of microbial protein synthesis from ARS which did occur when RB+SBM was added to untreated straw. The quality of ammoniated rice straw could be improved through RB supplementation because of its positive effects on DM digestibility, nitrogen retention and microbial protein yield. However, the addition of RB+SBM to URS resulted to more efficient N utilization.

• Proceedings of the Korean Magnetic Resonance Society Conference
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• 1999.06a
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• pp.36-36
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• 1999